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Wyszukujesz frazę "real-time detection" wg kryterium: Temat


Tytuł:
Requirements of a hard formations early detection system in opencast lignite mines
Wymagania systemu wczesnego wykrywania twardych formacji skalnych w odkrywkowych kopalniach węgla brunatnego
Autorzy:
Michalakopoulos, T.
Apostolopoulos, G.
Kofakis, P.
Voulgarakis, A.
Menegaki, M.
Amolochitis, G.
Galetakis, M.
Roumpos, C.
Powiązania:
https://bibliotekanauki.pl/articles/170790.pdf
Data publikacji:
2018
Wydawca:
Poltegor-Instytut Instytut Górnictwa Odkrywkowego
Tematy:
continuous surface mining
opencast lignite mining
bucket wheel excavators
hard inclusions
real-time detection
geophysical methods
BEWEXMIN
ciągły system wydobywczy
odkrywkowe wydobycie węgla brunatnego
wielonaczyniowe koparki kołowe
twarde inkluzje
wykrywanie w czasie rzeczywistym
metody geofizyczne
Opis:
One of the frequent problems that needs to be addressed when mining coal deposits is the occurence of cohesive materials of high mechanical strength in relation to the other materials of the series. This problem is of particular importance in Europe where lignite deposits are exploited in large opencast mines utilizing Bucket Wheel Excavators (BWEs) as the main means of excavation. Often it is difficult or impossible to excavate these hard inclusions with BWEs. If their location has been determined by exploration in advance, they are usually blasted. But it is not uncommon to discover them when it is too late, that is when the BWE actually digs into them. To proactively address this problem a clear forward warning for the presence of hard inclusions is required. However, it is impossible to detect all hard inclusions present in a coal deposit by conventional exploration methods, like drilling and geological modeling. Even the results of an exceptionally extensive exploration project would have a high level of uncertainty. A different approach is to focus on the area where the actual problem may take place, namely the excavation face where the BWE is digging. If one could “see” a few cuts ahead of the face and detect hard inclusions, digging into them would be avoided. This can be achieved by developing a system mounted on the BWE, continuously surveying the excavation face and detecting in advance hard inclusions by using appropriate geophysical methods, thus warning for potential problems. In this paper the requirements and components of such a system are presented, based on the typical geologic setting, the specifications of the excavating equipment, the employed working methods, and the limitations of the available geophysical methods.
Jednym z częstych problemów, które należy rozwiązać przy wydobywaniu złóż węgla brunatnego, jest występowanie spoistych materiałów o wysokiej wytrzymałości mechanicznej w stosunku do innych utworów tworzących nadkład. Problem ten ma szczególne znaczenie w Europie, gdzie złoża węgla brunatnego są eksploatowane w dużych odkrywkowych kopalniach wykorzystujących wielonaczyniowe koparki kołowe (BWE) jako maszyny podstawowe. Często trudne lub nawet niemożliwe jest urobienie tych twardych inkluzji przy pomocy tych koparek. Jeśli lokalizacja wtrąceń została wcześniej określona poprzez rozpoznanie, są one zwykle urabiane z użyciem materiałów wybuchowych. Często jednak zdarzają się przypadki nie wykrycia takiej struktury do momentu, gdy koło urabiające koparki już w nie uderzy. Aby umożliwić zareagowanie na ten problem, konieczne jest otrzymanie wyraźnego ostrzeżenia o obecności twardych inkluzji. Jednak niemożliwe jest wykrycie wszystkich twardych inkluzji obecnych w złożu węgla konwencjonalnymi metodami poszukiwawczymi, takimi jak wiercenie i modelowanie geologiczne. Nawet wyniki wyjątkowo rozległego projektu poszukiwawczego będą miały wysoki poziom niepewności. Innym podejściem jest skupienie się na obszarze, na którym może wystąpić faktyczny problem, a mianowicie w zabierce, w której koparka wielonaczyniowa pracuje. Możliwość rozpoznania kilku pasm przed czołem zabierki i wykrycia twardych wtrąceń, pozwoliłaby na uniknięcia uderzenia w nie organu urabiającego koparki. Można to osiągnąć, opracowując system zainstalowany bezpośrednio na koparce, który będzie prowadził ciągły monitoring w przodku zabierki i pozwoli na wyprzedzające wykrycie twardych wtrąceń za pomocą odpowiednich metod geofizycznych, ostrzegając w ten sposób przed potencjalnymi problemami. W niniejszym artykule przedstawiono wymagania i elementy takiego Systemu, oparte na typowej budowie geologicznej, specyfikacjach maszyn podstawowych, zastosowanych metodach pracy oraz ograniczeniach dostępnych metod geofizycznych.
Źródło:
Górnictwo Odkrywkowe; 2018, 59, 4; 51-60
0043-2075
Pojawia się w:
Górnictwo Odkrywkowe
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Development of TaqMan-based real-time PCR assay based on the E1 genefor the quantitative detection of the Getah virus
Autorzy:
Lin, A.
Hu, X.
Cui, S.
Yang, T.
Zhang, Z.
Li, P.
Guo, M.
Lu, Y.
Powiązania:
https://bibliotekanauki.pl/articles/16647453.pdf
Data publikacji:
2023
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
Getah virus
real-time PCR
TaqMan
detection
Opis:
To develop a sensitive, specific, and rapid approach for the detection Getah virus (GETV), a set of primers targeting the conserved region of the E1 gene was created. The TaqMan-based real-time PCR method for GETV detection was developed by optimizing the reaction conditions. The method demonstrated excellent specificity, and amplification did not occur with the causative agents of all prevalent swine viral infections (CSFV, PRRSV, PRV, PEDV, PTV, and JEV), except GETV. Additionally, upon assessing the sensitivity of the method, the minimum detection limit for GETV was found to be 5.94 copies/μL, which is 10 times higher than that of the traditional PCR approach. Further, the intra- and inter-assay variation coefficients were less than 1%, demonstrating good repeatability. Moreover, GETV was found in 10 of the 20 field serum samples using real-time PCR but only in three of the samples using traditional PCR. Consequently, the first GETV TaqMan-based real-time PCR approach based on the E1 gene was developed for GETV pathogenic diagnoses, and this exhibited high specificity, sensitivity, and repeatability. This assay is practical for the pathogenic diagnosis and epidemiology of GETV.
Źródło:
Polish Journal of Veterinary Sciences; 2023, 26, 1; 21-28
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
PCR and real-time PCR assays to detect fungi of Alternaria alternata species
Autorzy:
Kordalewska, Milena
Brillowska-Dąbrowska, Anna
Jagielski, Tomasz
Dworecka-Kaszak, Bożena
Powiązania:
https://bibliotekanauki.pl/articles/1038891.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Alternaria alternata
detection
identification
PCR
real-time PCR
Opis:
Fungi of the Alternaria genus are mostly associated with allergic diseases. However, with a growing number of immunocompromised patients, these fungi, with A. alternata being the most prevalent one, are increasingly recognized as etiological agents of infections (phaeohyphomycoses) in humans. Nowadays, identification of Alternaria spp. requires their pure culture and is solely based on morphological criteria. Clinically, Alternaria infections may be indistinguishable from other fungal diseases. Therefore, a diagnostic result is often delayed or even not achieved at all. In this paper we present easy to perform and interpret PCR and real-time PCR assays enabling detection of A. alternata species. On the basis of alignment of β-tubulin gene sequences, A. alternata-specific primers were designed. DNA from fungal isolates, extracted in a two-step procedure, were used in PCR and real-time PCR assays followed by electrophoresis or melting temperature analysis, respectively. The assays specificity was confirmed, since positive results were obtained for all A. alternata isolates, and no positive results were obtained neither for other molds, dermatophytes, yeast-like fungi, nor human DNA. The assays developed here enable fast and unambiguous identification of A. alternata pathogens.
Źródło:
Acta Biochimica Polonica; 2015, 62, 4; 707-712
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Real-time Video Vectorization Method for the Purpose of Surroundings Recognition and Mobile Robot Navigation
Autorzy:
Zachara, M.
Tadeusiewicz, R.
Powiązania:
https://bibliotekanauki.pl/articles/384314.pdf
Data publikacji:
2008
Wydawca:
Sieć Badawcza Łukasiewicz - Przemysłowy Instytut Automatyki i Pomiarów
Tematy:
robot vision
surroundings recognition
real time navigation
edge detection
Opis:
In thise paper a very simple method of the visual information interpretation for recognition and navigation purposes is presented and discussed. The proposed method consists of six steps: image acquisition, edge detection, fast edge vectorization using a high number of short preliminary vectors, aggregation of the preliminary vectors into the form of final vectors. The next stages of the visualthe visual information interpretation for recognition and navigation purposes will be description of the objects’ shapes by means of the final vectors, object recognition and/or robot navigation on the base of comparison between actual shape description and templates memorized during the programming/training process, but they are not discussed in this paper. The main advantage of the proposed method is a simple and time-effective algorithm, which can be performed in real time also by a simple and cheap processor, working as a “brain” of the considered robot.
Źródło:
Journal of Automation Mobile Robotics and Intelligent Systems; 2008, 2, 2; 10-17
1897-8649
2080-2145
Pojawia się w:
Journal of Automation Mobile Robotics and Intelligent Systems
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Detection and differentiation of Newcastle disease virus and influenza virus by using duplex real-time PCR
Autorzy:
Nidzworski, Dawid
Wasilewska, Edyta
Smietanka, Krzysztof
Szewczyk, Bogusław
Minta, Zenon
Powiązania:
https://bibliotekanauki.pl/articles/1039554.pdf
Data publikacji:
2013
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Newcastle disease virus
influenza virus
detection
differentiation
real-time PCR
duplex
Opis:
Newcastle disease virus (NDV), member of the Paramyxoviridae family and avian influenza virus (AIV), member of the Orthomyxoviridae family, are two main avian pathogens causing serious economic problems in poultry health. Both are enveloped, single-stranded, negative-sense RNA viruses and cause similar symptoms, ranging from sub-clinical infections to severe diseases, including decrease in egg production, acute respiratory syndrome, and high mortality. Similar symptoms hinder the differentiation of infection with the two viruses by standard veterinary procedures like clinical examination or necropsy. To overcome this problem, we have developed a new duplex real-time PCR assay for the detection and differentiation of these two viruses. Eighteen NDV strains, fourteen AIV strains, and twelve other (negative control) strains viruses were isolated from allantoic fluids of specific pathogen-free (SPF), embryonated eggs. Four-weeks-old SPF chickens were co-infected with both viruses (NDV - LaSota and AIV - H7N1). Swabs from cloaca and trachea were collected and examined. The results obtained in this study show that by using duplex real-time PCR, it was possible to detect and distinguish both viruses within less than three hours and with high sensitivity, even in case a bird was co-infected. Additionally, the results show the applicability of the real-time PCR assay in laboratory practice for the identification and differentiation of Newcastle disease and influenza A viruses in birds.
Źródło:
Acta Biochimica Polonica; 2013, 60, 3; 475-480
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Optimization of flotation, DNA extraction and PCR methods for detection of Toxoplasma gondii oocysts in cat faeces
Autorzy:
Sroka, J.
Karamon, J.
Dutkiewicz, J.
Wójcik-Fatla, A.
Cencek, T.
Powiązania:
https://bibliotekanauki.pl/articles/2081971.pdf
Data publikacji:
2018
Wydawca:
Instytut Medycyny Wsi
Tematy:
flotation
Toxoplasma gondii
faeces
Real time PCR
nested PCR
cats
oocysts detection
Opis:
Introduction and objective. The aim of the study was to compare the effectiveness of selected oocysts concentration methods, DNA extraction protocols and PCR assays targeting the B1 gene, for the development of procedures which would be effective and useful in laboratory practice for the detection of T. gondii in faecal samples from cats. Materials and method. In order to compare the influence of the flotation fluids on microscopy and PCR detection of T. gondii, saturated solutions of saccharose, MgSO4, ZnSO4 and NaNO3 were used. To determine the sensitivity of PCR tests used: Real time PCR (RT) and nested PCR, water samples spiked with T. gondii tachyzoites and oocysts were tested. DNA was extracted using DNeasy Blood and Tissue Kit (Qiagen) (K1). The same PCR tests were used to assess the efficacy of T. gondii DNA detection in samples of cat faeces spiked with oocysts, using DNA extraction by a K1 set and a K2 set (QIamp DNA Stool Mini Kit, (Qiagen). Results. The initial results showed that NaNO3 was most useful as a flotation fluid due to the lack negative effect on the oocysts and amplification efficacy in PCR. The level of detection for water samples (100 μl) was determined as 100 tachyzoites and 1–50 oocysts in RT, and 2–20 oocysts in nested PCR. The limit of detection (LD) for stool samples (250 mg) spiked with oocysts, where the K1 set was used, determined as 250 and 5 oocysts in RT and nested PCR, respectively. For samples extracted with the K2 set, LD in RT was determined as 1–50 oocysts (depending on the variant) and 50 oocysts in nested PCR. Conclusions. The most effective methods for detection of T. gondii in cat faeces seem to be centrifugal flotation with NaNO3, followed by DNA extraction with removing of inhibitors (K2 set) and Real Time PCR targeting B1 gene.
Źródło:
Annals of Agricultural and Environmental Medicine; 2018, 25, 4; 680-685
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
HybProbes-based real-time PCR assay for rapid detection of equine herpesvirus type 2 DNA
Autorzy:
Osinska, E.
Golke, A.
Slonska, A.
Cymerys, J.
Banbura, M.W.
Dzieciatkowski, T.
Powiązania:
https://bibliotekanauki.pl/articles/30252.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
rapid detection
equine herpesvirus
real-time polymerase chain reaction
horse
herpesvirus
Gammaherpesvirinae
Rhadinovirus
veterinary virology
Opis:
Equid herpesvirus type 2 (EHV-2) together with equid herpesvirus type 5 are members of Gammaherpesvirinae subfamily, genus Rhadinovirus. EHV-2 is one of major agents causing diseases of horses common worldwide. A possible role of EHV-2 in reactivating latent equid herpesvirus type-1 has been suggested, because reactivation of latent EHV-1 was always accompanied by EHV-2 replication. Variety techniques, including cell culture, PCR and its modifications, have been used to diagnose EHV-2 infections. The aim of this study was to develop, optimize and determine specificity of real-time PCR (qPCR) for EHV-2 DNA detection using HybProbesR chemistry and to evaluate clinical samples with this method. The analytical sensitivity of assay was tested using serial dilutions of viral DNA in range between 70 and 7x105 copies/ml. The limit of detection (LOD) was calculated using probit analysis and was determined as 56 copies/ml. In further studies 20 different clinical samples were tested for the presence of EHV-2. Described in-house qPCR method detected viral DNA in 5 of 20 specimens used. The results of this work show that developed HybProbes-based real-time PCR assay is very reliable and valuable for detection and quantification of equid herpesvirus type 2 DNA in different clinical samples. The high level of sensitivity, accuracy and rapidity provided by the LightCycler 2.0 instrument are favorable for the use of this system in the detection of EHV-2 DNA in veterinary virology.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Real-Time Market Abuse Detection with a Stochastic Parameter Model
Autorzy:
Cholewiński, Radosław
Powiązania:
https://bibliotekanauki.pl/articles/483319.pdf
Data publikacji:
2009
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Market abuse detection
insider trading
real-time analysis
timevarying parameters
uni- and bivariate GARCH processes
Opis:
This paper develops a new model of market abuse detection in real time. Market abuse is detected, as Minenna (2003) proposed, on the basis of prediction intervals. The model structure is based on the discrete-time, extended market model introduced by Monteiro, Zaman, Leitterstorf (2007) to analyze the market cleanliness. Parameters of the expected return equation are assumed, however, to be time-varying and estimated under the state-space framework using the extended Kalman filter postulated by Chou, Engle, Kane (1992) to capture the GARCH effect in returns. QML estimation is performed on intraday data; its utilization is proposed as an alternative to the continuous time modeling by Minenna (2003). This framework is generalized to the bivariate case which enables the analysis of daily open/close data. The paper also extends procedures of the statistical verification of the estimated state-space model to include the uncertainty arising from time-invariant parameters.
Źródło:
Central European Journal of Economic Modelling and Econometrics; 2009, 1, 3; 261-284
2080-0886
2080-119X
Pojawia się w:
Central European Journal of Economic Modelling and Econometrics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Canny edge detection based real-time intelligent parking management system
Autorzy:
Trivedi, Janak
Devi, Mandalapu Sarada
Dhara, Dave
Powiązania:
https://bibliotekanauki.pl/articles/197804.pdf
Data publikacji:
2020
Wydawca:
Politechnika Śląska. Wydawnictwo Politechniki Śląskiej
Tematy:
Raspberry Pi
parking
edge detection
Python
real-time
sensors
parkowanie
wykrywanie krawędzi
Pyton
czas rzeczywisty
czujniki
Opis:
Real-time traffic monitoring and parking are very important aspects for a better social and economic system. Python-based Intelligent Parking Management System (IPMS) module using a USB camera and a canny edge detection method was developed. The current situation of real-time parking slot was simultaneously checked, both online and via a mobile application, with a message of Parking “Available” or “Not available” for 10 parking slots. In addition, at the time entering in parking module, gate open and at the time of exit parking module, the gate closes automatically using servomotor and sensors. Results are displayed in figures with the proposed method flow chart.
Źródło:
Zeszyty Naukowe. Transport / Politechnika Śląska; 2020, 106; 197-208
0209-3324
2450-1549
Pojawia się w:
Zeszyty Naukowe. Transport / Politechnika Śląska
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A simple method for extracting DNA from rhododendron plants infected with Phytophthora spp. for use in PCR
Autorzy:
Trzewik, A.
Nowak, K.J.
Orlikowska, T.
Powiązania:
https://bibliotekanauki.pl/articles/66480.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
simple method
DNA extraction
rhododendron
leaf
plant infection
Phytophthora
polymerase chain reaction
detection
real-time PCR method
Opis:
Among the numerous protocols that describe the extraction of DNA, those relating to the isolation of DNA from infected plants, are rare. This study describes a rapid and reliable method of extracting a high quality and quantity of DNA from rhododendron leaves artificially infected with Phytophthora cactorum, P. cambivora, P. cinnamomi, P. citrophthora, and P. plurivora. The use of the modified Doyle and Doyle protocol (1987) allowed us to obtain high quantity and quality DNA (18.26 μg from 100 mg of the fresh weight of infected leaves at the ratios of A260/280 and A260/230 – 1.83 and 1.72, respectively), suitable for conventional polymerase chain reaction (PCR) and real-time PCR amplifications.
Źródło:
Journal of Plant Protection Research; 2016, 56, 1
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł

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