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Wyświetlanie 1-15 z 21
Tytuł:
Anaplasma phagocytophilum infection of red foxes [Vulpes vulpes]
Autorzy:
Karbowiak, G
Vichova, B.
Majlathova, V.
Hapunik, J.
Pet'ko, B.
Powiązania:
https://bibliotekanauki.pl/articles/50073.pdf
Data publikacji:
2009
Wydawca:
Instytut Medycyny Wsi
Tematy:
Polska
Mazovia region
parasite
Anaplasma phagocytophilum
infection
animal disease
red fox
Vulpes vulpes
16S rRNA gene
msp4 gene
Źródło:
Annals of Agricultural and Environmental Medicine; 2009, 16, 2; 299-300
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Application of molecular techniques to taxonomic studies
Autorzy:
Lesicki, A.
Szweykowska-Kulinska, Z.
Powiązania:
https://bibliotekanauki.pl/articles/84088.pdf
Data publikacji:
1999
Wydawca:
Uniwersytet Mikołaja Kopernika. Wydział Biologii i Ochrony Środowiska. Stowarzyszenie Malakologów Polskich
Tematy:
application
molecular technique
taxonomy
genome organization
phylogenetic analysis
rRNA gene
animal genome
mitochondrial genome
nuclear genome
DNA hybridization
mollusc
phylogenesis
Źródło:
Folia Malacologica; 1999, 07, 4
1506-7629
Pojawia się w:
Folia Malacologica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Bacterial community structure influenced by Coscinodiscus sp. in the Vistula river plume
Autorzy:
Ameryk, A.
Hahnke, R.L.
Gromisz, S.
Kownacka, J.
Zalewski, M.
Szymanek, L.
Calkiewicz, J.
Dunalska, J.
Harder, J.
Powiązania:
https://bibliotekanauki.pl/articles/47978.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Instytut Oceanologii PAN
Tematy:
planktonic bacteria
Coscinodiscus
phytoplankton community
primary production
Vistula River
river plume
Baltic Sea
terminal restriction fragment length polymorphism
DNA extraction
16S rRNA gene
dissolved organic matter
Źródło:
Oceanologia; 2014, 56, 4
0078-3234
Pojawia się w:
Oceanologia
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Bacterial species identification
Autorzy:
Kshikhundo, Ronald
Itumhelo, Shayalethu
Powiązania:
https://bibliotekanauki.pl/articles/1153736.pdf
Data publikacji:
2016
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
16S rRNA gene
Bacteria
Biolog
Gram staining
MALDI-TOF MS
RiboPrinter
computational tools
fatty acids
identification
metagenomics
morphology
Opis:
The traditional methods of bacterial identification are based on observation of either the morphology of single cells or colony characteristics. However, the adoption of newer and automated methods offers advantage in terms of rapid and reliable identification of bacterial species. The review provides a comprehensive appreciation of new and improved technologies such fatty acid profiling, sequence analysis of the 16S rRNA gene, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF), metabolic finger profiling using BIOLOG, ribotyping, together with the computational tools employed for querying the databases that are associated with these identification tools and high throughput genomic sequencing in bacterial identification. It is evident that with the increase in the adoption of new technologies, bacterial identification is becoming easier.
Źródło:
World News of Natural Sciences; 2016, 3; 26-38
2543-5426
Pojawia się w:
World News of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biodiversity and antibiotic resistance of bacteria isolated from tap water in Wrocław, Poland
Autorzy:
Leginowicz, M.
Siedlecka, A.
Piekarska, K.
Powiązania:
https://bibliotekanauki.pl/articles/207741.pdf
Data publikacji:
2018
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
water supply
drinking water
biological diversity
16S rRNA gene
systemy zasilania
woda pitna
różnorodność biologiczna
Opis:
Microbial contamination and biodiversity were determined for the drinking water samples collected from selected points of the water supply system in Wroclaw, Poland. All tested samples met the requirements of Polish law, i.e., the Regulation of the Minister of Health. However, the antibiotic resistant bacteria were found. The correlation between the distance of sampling points from water treatment plants and their microbial contamination was not established. Nevertheless, the Na Grobli treatment plant seemed to produce water of higher microbial quality than the Mokry Dwor treatment plant at the moment of sampling. The identification of representative isolates was performed with 16S rRNA gene sequencing and MALDI-TOF mass spectrometry and the results of these two methods were compared, indicating some discrepancies. Nevertheless, bacteria dwelling in drinking water in Wroclaw belonged to the phyla Actinobacteria, Proteobacteria (alpha-, beta-, gamma-Proteobacteria) and Firmicutes . The determination of antibiotic resistance profiles showed that 12 from 17 tested isolates revealed resistance to at least one antibiotic and two strains were multi-drug-resistant.
Źródło:
Environment Protection Engineering; 2018, 44, 4; 85-98
0324-8828
Pojawia się w:
Environment Protection Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparison of PCR-DGGE and Nested-PCR-DGGE Approach for Ammonia Oxidizers Monitoring in Membrane Bioreactors’ Activated Sludge
zalety i wady wykorzystywania techniki nested-PCR w monitoringu bakterii utleniających amoniak w osadzie czynnym bioreaktora membranowego
Autorzy:
Ziembińska-Buczyńska, A.
Wiszniowski, J.
Ciesielski, S.
Powiązania:
https://bibliotekanauki.pl/articles/204755.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
ammonia oxidizing bacteria
AOB
16S rRNA gene
Polymerase Chain Reaction – Denaturing Gradient Gel Electrophoresis
PCR-DGGE
nested-PCR
utlenianie amoniaku
bakteria utleniająca amoniak
gen kodujący 16S rRNA
Opis:
Nitritation, the first stage of ammonia removal process is known to be limiting for total process performance. Ammonia oxidizing bacteria (AOB) which perform this process are obligatory activated sludge habitants, a mixture consisting of Bacteria, Protozoa and Metazoa used for biological wastewater treatment. Due to this fact they are an interesting bacterial group, from both the technological and ecological point of view. AOB changeability and biodiversity analyses both in wastewater treatment plants and lab-scale reactors are performed on the basis of 16S rRNA gene sequences using PCR-DGGE (Polymerase Chain Reaction – Denaturing Gradient Gel Electrophoresis) as a molecular biology tool. AOB researches are usually led with nested PCR. Because the application of nested PCR is laborious and time consuming, we have attempted to check the possibility of using only first PCR round to obtain DGGE fingerprinting of microbial communities. In this work we are comparing the nested and non-nested PCR-DGGE monitoring of an AOB community and presenting advantages and disadvantages of both methods used. The experiment revealed that PCR technique is a very sensitive tool for the amplification of even a minute amount of DNA sample. But in the case of nested-PCR, the sensitivity is higher and the template amount could be even smaller. The nested PCR-DGGE seems to be a better tool for AOB community monitoring and complexity research in activated sludge, despite shorter fragments of DNA amplification which seems to be a disadvantage in the case of bacteria identification. It is recommended that the sort of analysis approach should be chosen according to the aim of the study: nested-PCR-DGGE for community complexity analysis, while PCR-DGGE for identification of the dominant bacteria.
Nitiritacja – pierwszy etap nitryfikacji, jest uznawany za krok limitujący przebieg całości procesu utleniania amoniaku. Bakterie utleniające amoniak (ang. ammonia oxidizing bacteria, AOB), które prowadzą ten proces są stałymi mieszkańcami osadu czynnego – mieszaniny bakterii, Protozoa i Metazoa, wykorzystywanych do biologicznego oczyszczania ścieków. Z tego powodu są one interesujące zarówno z punktu widzenia technologii, jak i ekologii mikroorganizmów. Analizy zmienności i bioróżnorodności bakterii utleniających amoniak, zarówno w oczyszczalni ścieków, jak i w reaktorach w skali laboratoryjnej, są prowadzone w oparciu o sekwencje genu kodującego 16S rRNA z użyciem metody biologii molekularnej, jaką jest PCR-DGGE (Polymerase Chain Reaction – Denaturing Gradient Gel Electrophoresis). Analizy te są zazwyczaj prowadzone techniką tzw. nested-PCR. Ze względu na fakt, że metoda ta wymaga większego nakładu pracy i czasu, niż tradycyjny jednoetapowy PCR (ang. non-nested PCR) podjęto próbę sprawdzenia możliwości zastosowania techniki jednoetapowego PCR do uzyskania wzorów prążkowych DGGE bakterii utleniających amoniak. W tej pracy zaprezentowano wyniki analizy PCR-DGGE z użyciem technik nested i non-nested PCR oraz podjęto próbę wykazania ich wad i zalet.
Źródło:
Archives of Environmental Protection; 2014, 40, 4; 31-38
2083-4772
2083-4810
Pojawia się w:
Archives of Environmental Protection
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparison of the nuclear organiser region activity in four taxa of the family Canidae
Autorzy:
Pienkowska, A
Zagalska, M.
Powiązania:
https://bibliotekanauki.pl/articles/2041199.pdf
Data publikacji:
2001
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
nuclear organiser region activity
gene cluster
rRNA
sex chromosome
genetics
Canidae
racoon dog
karyotype
rRNA gene
Źródło:
Journal of Applied Genetics; 2001, 42, 4; 493-501
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Diagnosis of Pneumocystis carinii infection by a serum polymerase chain reaction
Autorzy:
Golab, E.
Sobolewska, A.
Dzbenski, T.
Bitkowska, E.
Powiązania:
https://bibliotekanauki.pl/articles/836353.pdf
Data publikacji:
1998
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
Pneumocystis carinii
infection
serum
diagnosis
laboratory diagnosis
pneumonia
polymerase chain reaction
rRNA gene
T cell
Źródło:
Annals of Parasitology; 1998, 44, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Electron Microscopical Investigations of a New Species of the Genus Sappinia (Thecamoebidae, Amoebozoa), Sappinia platani sp. nov., Reveal a Dictyosome in this Genus
Autorzy:
WYLEZICH, Claudia
Kudryavtsev, Alexander
Michel, Rolf
Corsaro, Daniele
Walochnik, Julia
Powiązania:
https://bibliotekanauki.pl/articles/763632.pdf
Data publikacji:
2015
Wydawca:
Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
Tematy:
Sappinia platani sp. nov., Amoebozoa, Thecamoebidae, SSU rRNA gene, diversity, dictyosome, glycocalyx, endocytobionts
Opis:
The genus Sappinia belongs to the family Thecamoebidae within the Discosea (Amoebozoa). For long time the genus comprised only two species, S. pedata and S. diploidea, based on morphological investigations. However, recent molecular studies on gene sequences of the small subunit ribosomal RNA (SSU rRNA) gene revealed a high genetic diversity within the genus Sappinia. This indicated a larger species richness than previously assumed and the establishment of new species was predicted. Here, Sappinia platani sp. nov. (strain PL-247) is described and ultrastructurally investigated. This strain was isolated from the bark of a sycamore tree (Koblenz, Germany) like the re-described neotype of S. diploidea. The new species shows the typical characteristics of the genus such as flattened and binucleate trophozoites with a differentiation of anterior hyaloplasm and without discrete pseudopodia as well as bicellular cysts. Additionally, the new species possesses numerous endocytobionts and dictyosomes. The latter could not be found in previous EM studies of the genus Sappinia. Standing forms, a character of the species S. pedata, could be formed on older cultures of the new species but appeared extremely seldom. A loose layer of irregular, bent hair-like structures cover the plasma membrane dissimilar to the glycocalyx types as formerly detected in other Sappinia strains.
Źródło:
Acta Protozoologica; 2015, 54, 1
1689-0027
Pojawia się w:
Acta Protozoologica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
First insight into microbial community composition in a phosphogypsum waste heap soil
Autorzy:
Zielińska, Sylwia
Radkowski, Piotr
Ossowski, Tadeusz
Ludwig-Gałęzowska, Agnieszka
Łoś, Joanna
Łoś, Marcin
Powiązania:
https://bibliotekanauki.pl/articles/1038561.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
microbial community
16S rRNA gene
soil sample
postproduction waste
phosphogypsum
reclamation
Opis:
The aim of this study was to investigate the soil microbial communities of a phosphogypsum waste heap. The soil microbial community structures can differ over time, as they are affected by the changing environmental conditions caused by a long-term exposure to different kinds of pollutions, like is the case of soil in the post-production waste area in Wiślinka (in the northern part of Poland) currently undergoing restoration. Our analyses indicated that the most abundant phyla were Proteobacteria, Acidobacteria, and Actinobacteria, and generally such an abundance is common for most of the studied soils. The most dominant class were Alphaproteobacteria, with their participation in 33.46% of the total reads. Among this class, the most numbered order was Sphingomonadales, whereas among this order the Sphingomonadaceae family was the most abundant one. The Sphingomonadaceae family is currently in the center of interest of many researchers, due to the ability of some of its members to utilize a wide range of naturally occurring organic compounds and many types of environmental contaminants. This kind of knowledge about microbial populations can support efforts in bioremediation and can improve monitoring changes in the contaminated environments.
Źródło:
Acta Biochimica Polonica; 2017, 64, 4; 693-698
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
FISH mapping of 18S-5.8S-26S rRNA genes and fluorochrome banding in the triploid viviparous onion Allium x cornutum Clementi ex Visiani, 1842
Autorzy:
Lepen, I.
Puizina, J.
Powiązania:
https://bibliotekanauki.pl/articles/19898.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
rRNA gene
fluorochrome banding
triploid
Allium cepa var.viviparum
fluorescence in situ hybridization
FISH zob.fluorescence in situ hybridisation
mapping
onion
Allium
viviparous onion
Allium cepa var.proliferum
Opis:
Triploid viviparous onions [Allium × cornutum Clementi ex Visiani 1842, syn Allium cepa L. var. viviparum Metzg. (ALEF.), auct.] (2n = 3x = 24), are known in some countries only as rare relict crops. In other parts of the world they are still traditionally or even commercially cultivated. In previous cytogenetic studies of the Croatian triploid viviparous onion Ljutika, Giemsa C-banding, chromosome pairing analysis during meiosis, and genomic hybridization in situ indicated a complex hybrid with highly heterozygous karyotype structure, with possible triparental genome organization. This study continues an analysis of the karyotype structure of Ljutika. Staining with fluorochromes CMA3 (Chromomycin A3) and DAPI (4,6-diamidino-2-phenylindole) confirmed previous results from Giemsa C-banding and revealed GC-rich heterochromatic regions associated mainly with chromosome ends and nucleolus organizing regions (NORs), and only a few interstitial bands. FISH mapping of the ribosomal 18S-5.8S-26S genes revealed two major rDNA signals on the short arms of two subtelocentric satellite chromosomes in almost all metaphase plates of Ljutika. The largest subtelocentric chromosome lacked rDNA signals. A significantly smaller rDNA signal was occasionally located on one small submetacentric chromosome. These results are in agreement with previously published results from identification of NORs by silverstaining technique, which confirmed a maximum three nucleoli in interphase nuclei. We discuss the molecular mechanisms underlying rearrangements and activity of ribosomal genes in the triploid karyotype.
Źródło:
Acta Biologica Cracoviensia. Series Botanica; 2011, 53, 1
0001-5296
Pojawia się w:
Acta Biologica Cracoviensia. Series Botanica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Isolation and characterization of biosurfactants-producing bacteria isolated from palm oil industry and evaluation for biosurfactants production using low-cost substrates
Autorzy:
Saisa-Ard, K.
Manerrat, S.
Saimmai, A.
Powiązania:
https://bibliotekanauki.pl/articles/81283.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
isolation
biosurfactant
phylogenetic analysis
palm oil
contaminated soil
surface tension
phylogenetic position
16S rRNA gene
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Microbial biodiversity in arable soils is affected by agricultural practices
Autorzy:
Wolińska, Agnieszka
Górniak, Dorota
Zielenkiewicz, Urszula
Goryluk-Salmonowicz, Agata
Kuźniar, Agnieszka
Stępniewska, Zofia
Błaszczyk, Mieczysław
Powiązania:
https://bibliotekanauki.pl/articles/972735.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Instytut Agrofizyki PAN
Tematy:
dgge
16s rrna gene
simpson diversity
bacterial communities
arable soils
Opis:
The aim of the study was to examine the differences in microbial community structure as a result of agricultural practices. Sixteen samples of cultivated and the same number of non-cultivated soils were selected. Gel bands were identified using the GelCompar software to create the presence-absence matrix, where each band represented a bacterial operational taxonomic unit. The data were used for principal-component analysis and additionally, the Shannon-Weaver index of general diversity, Simpson index of dominance and Simpson index of diversity were calculated. Denaturing gradient gel electrophoresis profiles clearly indicated differentiation of tested samples into two clusters: cultivated and non-cultivated soils. Greater numbers of dominant operational taxonomic units (65) in non-cultivated soils were noted compared to cultivated soils (47 operational taxonomic units). This implies that there was a reduction of dominant bacterial operational taxonomic units by nearly 30% in cultivated soils. Simpson dominance index expressing the number of species weighted by their abundance amounted to 1.22 in cultivated soils, whereas a 3-fold higher value (3.38) was observed in non-cultivated soils. Land-use practices seemed to be a important factors affected on biodiversity, because more than soil type determined the clustering into groups.
Źródło:
International Agrophysics; 2017, 31, 2; 259-271
0236-8722
Pojawia się w:
International Agrophysics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular characterisation of Bulinus snails - intermediate hosts of schistosomes in Ogun State, south-western Nigeria
Autorzy:
Akinwale, O.
Oso, O.
Salawu, O.
Odaibo, A.
Tang, P.
Chen, T.-W.
Gyang, P.
Powiązania:
https://bibliotekanauki.pl/articles/84247.pdf
Data publikacji:
2015
Wydawca:
Uniwersytet Mikołaja Kopernika. Wydział Biologii i Ochrony Środowiska. Stowarzyszenie Malakologów Polskich
Tematy:
molecular characteristics
Bulinus
species identification
snail
host
intermediate host
schistosome
rRNA gene
schistosomiasis
Ogun State
Nigeria
Źródło:
Folia Malacologica; 2015, 23, 2
1506-7629
Pojawia się w:
Folia Malacologica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Morphological, genetic, chemical and ecophysiological characterisation of two Microcystis aeruginosa isolates from the Vistula Lagoon, Southern Baltic
Autorzy:
Mazur-Marzec, H.
Browarczyk-Matusiak, G.
Forycka, K.
Kobos, J.
Plinski, M.
Powiązania:
https://bibliotekanauki.pl/articles/48489.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Instytut Oceanologii PAN
Tematy:
Microcystis aeruginosa
Cyanoprokaryota
microcystin
mcy gene
morphology
Vistula Lagoon
Baltic Sea
eutrophic water
bloom
16S rRNA sequence
internal transcribed spacer
Opis:
The Vistula Lagoon (southern Baltic Sea) is a shallow and highly eutrophic water body, with frequent blooms of cyanobacteria dominated by Microcystis and Anabaena species. Two Microcystis strains, MK10.10 and MAKR0205, isolated from the lagoon were characterised in this work. The morphology of the isolates differed significantly with respect to cell size and their ability to form aggregates. Based on the 16S rRNA sequence and 16S-23S internal transcribed spacer (ITS) sequence, both isolates were classified as Microcystis aeruginosa. However, only one isolate, MK10.10, possessed the mcy genes responsible for microcystin biosynthesis and only this strain produced microcystins. The effects of environmental factors, such as light, temperature and salinity, on toxin production turned out to be minor. Under the culture conditions used in the experiments, the biomass of the toxic MK10.10 was always lower. Hybrid quadrupole-time-of-flight liquid chromatography/tandem mass spectrometry (QTOF-LC/MS/MS) was used to elucidate the structure of the microcystin (MC) variants produced by MK10.10. Based on molecular ion and fragmentation spectra, the toxins were identified as MC-LR, MC-VR and MC- HIlR. Our study confirmed that some morphological criteria could be useful in preliminarily assessing the potential toxicity of a Microcystis bloom.
Źródło:
Oceanologia; 2010, 52, 1; 127-146
0078-3234
Pojawia się w:
Oceanologia
Dostawca treści:
Biblioteka Nauki
Artykuł
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