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Tytuł:
Some corrosive bacteria isolated from the technogenic soil ecosystem in Chernihiv city (Ukraine)
Autorzy:
Tkachuk, Nataliia
Zelena, Liubov
Powiązania:
https://bibliotekanauki.pl/articles/2026000.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
16S rRNA gene
microbial induced corrosion
phenotypic characteristics
Opis:
The soil microbiome is exposed to technogenic influence during the operation of metal structures. There are quantitative and qualitative changes in the microbiota of the technogenic ecosystem. During the study of the technogenic soil ecosystem (ferrosphere), samples of which were taken in the field (Chernihiv, Ukraine: 51°29’58”N, 31°16’09”E), the presence of corrosively active microbial cenosis was established: sulfate-reducing, denitrifying, iron-reducing (using acetate as the only electron donor, and Fe (III) as the only electron acceptor) and ammonifying bacteria. The predominant representatives of corrosively active groups of bacteria were isolated. They were identified as Bacillus simplex, Streptomyces gardneri, Streptomyces canus (ammonifying bacteria), Fictibacillus sp. (ammonifying bacteria with iron-reducing ability), Anaerotignum (Clostridium) propionicum (organic acid-producing bacteria), Desulfovibrio oryzae (sulfate-reducing bacteria) based on some microbiological, physiological and biochemical, genetic features. Strains of heterotrophic and hemolitotrophic bacteria (individual representatives and their associations) isolated from the technogenic ecosystem can be used in both industrial and technological spheres. The interaction of isolated bacteria in the process of microbial induced corrosion is a prospect for further research.
Źródło:
Studia Quaternaria; 2021, 38, 2; 101-108
1641-5558
2300-0384
Pojawia się w:
Studia Quaternaria
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Morphology, Ciliary Pattern and Molecular Phylogeny of Trachelophyllum brachypharynx Levander, 1894 (Litostomatea, Haptoria, Spathidiida)
Autorzy:
JANG, Seok Won
VĎAČNÝ, Peter
SHAZIB, Shahed Uddin Ahmed
SHIN, Mann Kyoon
Powiązania:
https://bibliotekanauki.pl/articles/763656.pdf
Data publikacji:
2015
Wydawca:
Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
Tematy:
18S rRNA gene, dorsal brush, Korea, lepidosomes, Trachelophyllidae
Opis:
We isolated a relatively unknown haptorian ciliate, Trachelophyllum brachypharynx, in brackish water from the mouth of the Taehwa River, South Korea. The morphology of this isolate was studied using in vivo observation and protargol impregnation, and its evolutionary history was revealed by phylogenetic analysis of the 18S rRNA gene. The main features of T. brachypharynx include (i) a very narrowly fusiform and slightly contractile body about 380 × 40 μm in size; (ii) two ellipsoidal macronuclear nodules typically connected by a fine strand; (iii) a single terminal contractile vacuole; (iv) filiform extrusomes that are typically 30 µm long; (v) an average of 24 ciliary rows, with two of them anteriorly differentiated into an isostichad dikinetidal dorsal brush; and (vi) hat-shaped lepidosomes. Based on the 18S rRNA gene phylogeny, T. brachypharynx clustered together with Trachelophyllum sp. within the order Spathidiida. Furthermore, phylogenetic trees and networks indicate some members from the genera Enchelyodon and Spathidium as the nearest relatives of trachelophyllids. Therefore, based on the present molecular and comparative-morphological analyses, we suggested a hypothesis explaining how trachelophyllids may have evolved from a spathidiid-like ancestor via an enchelyodonid-like stage.
Źródło:
Acta Protozoologica; 2015, 54, 2
1689-0027
Pojawia się w:
Acta Protozoologica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Reconstruction of Evolutionary History of Pleurostomatid Ciliates (Ciliophora, Litostomatea, Haptoria): Interplay of Morphology and Molecules
Autorzy:
VĎAČNÝ, Peter
SHIN, Mann Kyoon
KIM, Ji Hye
JANG, Seok Won
SHAZIB, Shahed Uddin Ahmed
RAJTER, Ľubomír
Powiązania:
https://bibliotekanauki.pl/articles/763636.pdf
Data publikacji:
2015
Wydawca:
Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
Tematy:
18S rRNA gene, Acineria, Epiphyllidae fam. nov., Kentrophyllum, Korea
Opis:
Pleurostomatids are raptorial ciliates that form a very distinct group within the Haptoria. Traditionally, the order Pleurostomatida was divided into two families: the Amphileptidae with two perioral kineties and a suture formed by the right side ciliary rows, and the Litonotidae with three perioral kineties and without suture. However, molecular phylogenies depicted the “traditional” Amphileptidae as a paraphyletic assemblage nesting also the Litonotidae. To overcome this problem we have analyzed genealogy of pleurostomatids using morphological data and 18S rRNA gene sequences, including newly sequenced genera Acineria and Kentrophyllum. Specifically, we have combined a morphological and molecular approach and have used also some other phylogenetic tools such as phylogenetic networks, split spectrum analysis, quartet mapping as well as the likelihood method of tracing history of morphological characters. These analyses show that: (1) there are not two but three distinct pleurostomatid lineages – Epiphyllidae fam. nov., Amphileptidae and Litonotidae; (2) epiphyllids (Epiphyllum + Kentrophyllum) represent a basal pleurostomatid group which is defined by two perioral kineties, by the presence of a suture on both the right and the left side of the body, by the loss of the oral bulge extrusomes, and by the extrusome fringe extending all around the body except for the oral region; (3) the families Amphileptidae and Litonotidae are monophyletic each, and represent sister groups; (4) Acineria belongs to the Litonotidae, as already indicated by morphological data; (5) Loxophyllum is a monophyletic and crown genus of the Litonotidae; and (6) Litonotus is paraphyletic, which could be very likely caused by a rapid radiation event that did not allow primary nucleotide homologies to be fixed.
Źródło:
Acta Protozoologica; 2015, 54, 1
1689-0027
Pojawia się w:
Acta Protozoologica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparison of the nuclear organiser region activity in four taxa of the family Canidae
Autorzy:
Pienkowska, A
Zagalska, M.
Powiązania:
https://bibliotekanauki.pl/articles/2041199.pdf
Data publikacji:
2001
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
nuclear organiser region activity
gene cluster
rRNA
sex chromosome
genetics
Canidae
racoon dog
karyotype
rRNA gene
Źródło:
Journal of Applied Genetics; 2001, 42, 4; 493-501
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Microbial biodiversity in arable soils is affected by agricultural practices
Autorzy:
Wolińska, Agnieszka
Górniak, Dorota
Zielenkiewicz, Urszula
Goryluk-Salmonowicz, Agata
Kuźniar, Agnieszka
Stępniewska, Zofia
Błaszczyk, Mieczysław
Powiązania:
https://bibliotekanauki.pl/articles/972735.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Instytut Agrofizyki PAN
Tematy:
dgge
16s rrna gene
simpson diversity
bacterial communities
arable soils
Opis:
The aim of the study was to examine the differences in microbial community structure as a result of agricultural practices. Sixteen samples of cultivated and the same number of non-cultivated soils were selected. Gel bands were identified using the GelCompar software to create the presence-absence matrix, where each band represented a bacterial operational taxonomic unit. The data were used for principal-component analysis and additionally, the Shannon-Weaver index of general diversity, Simpson index of dominance and Simpson index of diversity were calculated. Denaturing gradient gel electrophoresis profiles clearly indicated differentiation of tested samples into two clusters: cultivated and non-cultivated soils. Greater numbers of dominant operational taxonomic units (65) in non-cultivated soils were noted compared to cultivated soils (47 operational taxonomic units). This implies that there was a reduction of dominant bacterial operational taxonomic units by nearly 30% in cultivated soils. Simpson dominance index expressing the number of species weighted by their abundance amounted to 1.22 in cultivated soils, whereas a 3-fold higher value (3.38) was observed in non-cultivated soils. Land-use practices seemed to be a important factors affected on biodiversity, because more than soil type determined the clustering into groups.
Źródło:
International Agrophysics; 2017, 31, 2; 259-271
0236-8722
Pojawia się w:
International Agrophysics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
First insight into microbial community composition in a phosphogypsum waste heap soil
Autorzy:
Zielińska, Sylwia
Radkowski, Piotr
Ossowski, Tadeusz
Ludwig-Gałęzowska, Agnieszka
Łoś, Joanna
Łoś, Marcin
Powiązania:
https://bibliotekanauki.pl/articles/1038561.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
microbial community
16S rRNA gene
soil sample
postproduction waste
phosphogypsum
reclamation
Opis:
The aim of this study was to investigate the soil microbial communities of a phosphogypsum waste heap. The soil microbial community structures can differ over time, as they are affected by the changing environmental conditions caused by a long-term exposure to different kinds of pollutions, like is the case of soil in the post-production waste area in Wiślinka (in the northern part of Poland) currently undergoing restoration. Our analyses indicated that the most abundant phyla were Proteobacteria, Acidobacteria, and Actinobacteria, and generally such an abundance is common for most of the studied soils. The most dominant class were Alphaproteobacteria, with their participation in 33.46% of the total reads. Among this class, the most numbered order was Sphingomonadales, whereas among this order the Sphingomonadaceae family was the most abundant one. The Sphingomonadaceae family is currently in the center of interest of many researchers, due to the ability of some of its members to utilize a wide range of naturally occurring organic compounds and many types of environmental contaminants. This kind of knowledge about microbial populations can support efforts in bioremediation and can improve monitoring changes in the contaminated environments.
Źródło:
Acta Biochimica Polonica; 2017, 64, 4; 693-698
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Electron Microscopical Investigations of a New Species of the Genus Sappinia (Thecamoebidae, Amoebozoa), Sappinia platani sp. nov., Reveal a Dictyosome in this Genus
Autorzy:
WYLEZICH, Claudia
Kudryavtsev, Alexander
Michel, Rolf
Corsaro, Daniele
Walochnik, Julia
Powiązania:
https://bibliotekanauki.pl/articles/763632.pdf
Data publikacji:
2015
Wydawca:
Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
Tematy:
Sappinia platani sp. nov., Amoebozoa, Thecamoebidae, SSU rRNA gene, diversity, dictyosome, glycocalyx, endocytobionts
Opis:
The genus Sappinia belongs to the family Thecamoebidae within the Discosea (Amoebozoa). For long time the genus comprised only two species, S. pedata and S. diploidea, based on morphological investigations. However, recent molecular studies on gene sequences of the small subunit ribosomal RNA (SSU rRNA) gene revealed a high genetic diversity within the genus Sappinia. This indicated a larger species richness than previously assumed and the establishment of new species was predicted. Here, Sappinia platani sp. nov. (strain PL-247) is described and ultrastructurally investigated. This strain was isolated from the bark of a sycamore tree (Koblenz, Germany) like the re-described neotype of S. diploidea. The new species shows the typical characteristics of the genus such as flattened and binucleate trophozoites with a differentiation of anterior hyaloplasm and without discrete pseudopodia as well as bicellular cysts. Additionally, the new species possesses numerous endocytobionts and dictyosomes. The latter could not be found in previous EM studies of the genus Sappinia. Standing forms, a character of the species S. pedata, could be formed on older cultures of the new species but appeared extremely seldom. A loose layer of irregular, bent hair-like structures cover the plasma membrane dissimilar to the glycocalyx types as formerly detected in other Sappinia strains.
Źródło:
Acta Protozoologica; 2015, 54, 1
1689-0027
Pojawia się w:
Acta Protozoologica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The Application of PCR Reaction for Identification of MHB Bacteria Species
Zastosowanie reakcji PCR do identyfikacji gatunkowej bakterii MHB
Autorzy:
Ząbkiewicz, A.
Myga-Nowak, M.
Bandurska, K.
Paczyńska, J.
Szybecka, A.
Krupa, P.
Powiązania:
https://bibliotekanauki.pl/articles/205412.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
mycorrhiza
helper bacteria
MHB
16S rRNA gene
mikoryza
bakterie wspomagające
gen 16sRNA
Opis:
This study characterizes mycorrhiza helper bacteria (MHB) from selected unpolluted locations as well as subjected to industrial emissions. To determine the species of bacteria isolated from the roots of ectomycorrhizal pine and birch, a method based on the sequence analysis of a 16S rRNA gene was used. The isolated bacteria were initially characterized by available biochemical methods and phenotypic observation. On the selected bacteria representatives isolation of DNA was performed, on which the PCR reaction was carried out. In this way amplified samples were automatically sequenced and the obtained results were compared to public databases. Among the isolated bacteria Pseudomonas fluorescens SBW25 and Burkholderia xenovorans LB400 species were dominant.
W pracy scharakteryzowano bakterie wspomagające mikoryzę pochodzące z wybranych terenów ekologicznie czystych oraz z terenów poddanych emisji przemysłowej. Przedstawiono wykorzystanie metody opartej na analizie sekwencji genu 16S rRNA do określenia przynależności gatunkowej bakterii wyizolowanych z korzeni ektomikoryzowych sosny i brzozy. Wyizolowane bakterie zostały wstępnie scharakteryzowane przy pomocy dostępnych metod biochemicznych i obserwacji fenotypowej. Dla wybranych przedstawicieli dokonano izolacji DNA, względem którego przeprowadzono reakcję PCR. Powielone w ten sposób próbki automatycznie zsekwencjonowano, a uzyskane sekwencje porównywano w ogólnodostępnych bazach danych. Wśród wyizolowanych bakterii dominowały gatunki Pseudomonas fluorescens SBW25 oraz Burkholderia xenovorans LB400.
Źródło:
Archives of Environmental Protection; 2014, 40, 2; 115-122
2083-4772
2083-4810
Pojawia się w:
Archives of Environmental Protection
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Diagnosis of Pneumocystis carinii infection by a serum polymerase chain reaction
Autorzy:
Golab, E.
Sobolewska, A.
Dzbenski, T.
Bitkowska, E.
Powiązania:
https://bibliotekanauki.pl/articles/836353.pdf
Data publikacji:
1998
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
Pneumocystis carinii
infection
serum
diagnosis
laboratory diagnosis
pneumonia
polymerase chain reaction
rRNA gene
T cell
Źródło:
Annals of Parasitology; 1998, 44, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biodiversity and antibiotic resistance of bacteria isolated from tap water in Wrocław, Poland
Autorzy:
Leginowicz, M.
Siedlecka, A.
Piekarska, K.
Powiązania:
https://bibliotekanauki.pl/articles/207741.pdf
Data publikacji:
2018
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
water supply
drinking water
biological diversity
16S rRNA gene
systemy zasilania
woda pitna
różnorodność biologiczna
Opis:
Microbial contamination and biodiversity were determined for the drinking water samples collected from selected points of the water supply system in Wroclaw, Poland. All tested samples met the requirements of Polish law, i.e., the Regulation of the Minister of Health. However, the antibiotic resistant bacteria were found. The correlation between the distance of sampling points from water treatment plants and their microbial contamination was not established. Nevertheless, the Na Grobli treatment plant seemed to produce water of higher microbial quality than the Mokry Dwor treatment plant at the moment of sampling. The identification of representative isolates was performed with 16S rRNA gene sequencing and MALDI-TOF mass spectrometry and the results of these two methods were compared, indicating some discrepancies. Nevertheless, bacteria dwelling in drinking water in Wroclaw belonged to the phyla Actinobacteria, Proteobacteria (alpha-, beta-, gamma-Proteobacteria) and Firmicutes . The determination of antibiotic resistance profiles showed that 12 from 17 tested isolates revealed resistance to at least one antibiotic and two strains were multi-drug-resistant.
Źródło:
Environment Protection Engineering; 2018, 44, 4; 85-98
0324-8828
Pojawia się w:
Environment Protection Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular characterisation of Bulinus snails - intermediate hosts of schistosomes in Ogun State, south-western Nigeria
Autorzy:
Akinwale, O.
Oso, O.
Salawu, O.
Odaibo, A.
Tang, P.
Chen, T.-W.
Gyang, P.
Powiązania:
https://bibliotekanauki.pl/articles/84247.pdf
Data publikacji:
2015
Wydawca:
Uniwersytet Mikołaja Kopernika. Wydział Biologii i Ochrony Środowiska. Stowarzyszenie Malakologów Polskich
Tematy:
molecular characteristics
Bulinus
species identification
snail
host
intermediate host
schistosome
rRNA gene
schistosomiasis
Ogun State
Nigeria
Źródło:
Folia Malacologica; 2015, 23, 2
1506-7629
Pojawia się w:
Folia Malacologica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Isolation and characterization of biosurfactants-producing bacteria isolated from palm oil industry and evaluation for biosurfactants production using low-cost substrates
Autorzy:
Saisa-Ard, K.
Manerrat, S.
Saimmai, A.
Powiązania:
https://bibliotekanauki.pl/articles/81283.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
isolation
biosurfactant
phylogenetic analysis
palm oil
contaminated soil
surface tension
phylogenetic position
16S rRNA gene
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Anaplasma phagocytophilum infection of red foxes [Vulpes vulpes]
Autorzy:
Karbowiak, G
Vichova, B.
Majlathova, V.
Hapunik, J.
Pet'ko, B.
Powiązania:
https://bibliotekanauki.pl/articles/50073.pdf
Data publikacji:
2009
Wydawca:
Instytut Medycyny Wsi
Tematy:
Polska
Mazovia region
parasite
Anaplasma phagocytophilum
infection
animal disease
red fox
Vulpes vulpes
16S rRNA gene
msp4 gene
Źródło:
Annals of Agricultural and Environmental Medicine; 2009, 16, 2; 299-300
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Bacterial species identification
Autorzy:
Kshikhundo, Ronald
Itumhelo, Shayalethu
Powiązania:
https://bibliotekanauki.pl/articles/1153736.pdf
Data publikacji:
2016
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
16S rRNA gene
Bacteria
Biolog
Gram staining
MALDI-TOF MS
RiboPrinter
computational tools
fatty acids
identification
metagenomics
morphology
Opis:
The traditional methods of bacterial identification are based on observation of either the morphology of single cells or colony characteristics. However, the adoption of newer and automated methods offers advantage in terms of rapid and reliable identification of bacterial species. The review provides a comprehensive appreciation of new and improved technologies such fatty acid profiling, sequence analysis of the 16S rRNA gene, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF), metabolic finger profiling using BIOLOG, ribotyping, together with the computational tools employed for querying the databases that are associated with these identification tools and high throughput genomic sequencing in bacterial identification. It is evident that with the increase in the adoption of new technologies, bacterial identification is becoming easier.
Źródło:
World News of Natural Sciences; 2016, 3; 26-38
2543-5426
Pojawia się w:
World News of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Application of molecular techniques to taxonomic studies
Autorzy:
Lesicki, A.
Szweykowska-Kulinska, Z.
Powiązania:
https://bibliotekanauki.pl/articles/84088.pdf
Data publikacji:
1999
Wydawca:
Uniwersytet Mikołaja Kopernika. Wydział Biologii i Ochrony Środowiska. Stowarzyszenie Malakologów Polskich
Tematy:
application
molecular technique
taxonomy
genome organization
phylogenetic analysis
rRNA gene
animal genome
mitochondrial genome
nuclear genome
DNA hybridization
mollusc
phylogenesis
Źródło:
Folia Malacologica; 1999, 07, 4
1506-7629
Pojawia się w:
Folia Malacologica
Dostawca treści:
Biblioteka Nauki
Artykuł

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