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Wyświetlanie 1-15 z 48
Tytuł:
Zastosowanie techniki PCR w badaniach bakteriologicznych
Applications of PCR in microbiology
Autorzy:
Adaszek, L.
Dziegiel, B.
Mazurek, L.
Winiarczyk, S.
Powiązania:
https://bibliotekanauki.pl/articles/859637.pdf
Data publikacji:
2018
Wydawca:
Krajowa Izba Lekarsko-Weterynaryjna
Tematy:
bakteriologia
diagnostyka bakteriologiczna
metody badan
lancuchowa reakcja polimerazy
16S rRNA
diagnostyka molekularna
Źródło:
Życie Weterynaryjne; 2018, 93, 04
0137-6810
Pojawia się w:
Życie Weterynaryjne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular characterisation of Bulinus snails - intermediate hosts of schistosomes in Ogun State, south-western Nigeria
Autorzy:
Akinwale, O.
Oso, O.
Salawu, O.
Odaibo, A.
Tang, P.
Chen, T.-W.
Gyang, P.
Powiązania:
https://bibliotekanauki.pl/articles/84247.pdf
Data publikacji:
2015
Wydawca:
Uniwersytet Mikołaja Kopernika. Wydział Biologii i Ochrony Środowiska. Stowarzyszenie Malakologów Polskich
Tematy:
molecular characteristics
Bulinus
species identification
snail
host
intermediate host
schistosome
rRNA gene
schistosomiasis
Ogun State
Nigeria
Źródło:
Folia Malacologica; 2015, 23, 2
1506-7629
Pojawia się w:
Folia Malacologica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Bacterial community structure influenced by Coscinodiscus sp. in the Vistula river plume
Autorzy:
Ameryk, A.
Hahnke, R.L.
Gromisz, S.
Kownacka, J.
Zalewski, M.
Szymanek, L.
Calkiewicz, J.
Dunalska, J.
Harder, J.
Powiązania:
https://bibliotekanauki.pl/articles/47978.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Instytut Oceanologii PAN
Tematy:
planktonic bacteria
Coscinodiscus
phytoplankton community
primary production
Vistula River
river plume
Baltic Sea
terminal restriction fragment length polymorphism
DNA extraction
16S rRNA gene
dissolved organic matter
Źródło:
Oceanologia; 2014, 56, 4
0078-3234
Pojawia się w:
Oceanologia
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Structure and functions of 5S rRNA.
Autorzy:
Barciszewska, Mirosława
Szymański, Maciej
Erdmann, Volker
Barciszewski, Jan
Powiązania:
https://bibliotekanauki.pl/articles/1044186.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
50S subunit structure
ribosomal 5S RNA
5S rRNA
crystal structure
Opis:
The ribosome is a macromolecular assembly that is responsible for protein biosynthesis in all organisms. It is composed of two-subunit, ribonucleoprotein particles that translate the genetic material into an encoded polypeptides. The small subunit is the site of codon-anticodon interaction between the messenger RNA (mRNA) and transfer RNA (tRNA) substrates, and the large subunit catalyses peptide bond formation. The peptidyltransferase activity is fulfilled by 23S rRNA, which means that ribosome is a ribozyme. 5S rRNA is a conserved component of the large ribosomal subunit that is thought to enhance protein synthesis by stabilizing ribosome structure. This paper shortly summarises new results obtained on the structure and function of 5S rRNA.
Źródło:
Acta Biochimica Polonica; 2001, 48, 1; 191-198
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Description of Epistylis camprubii n. sp., a Species Highly Tolerant to Ammonium and Nitrite
Autorzy:
Canals, Oriol
Salvadó, Humbert
Powiązania:
https://bibliotekanauki.pl/articles/763511.pdf
Data publikacji:
2016
Wydawca:
Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
Tematy:
Peritrichia, wastewater treatment, ciliates, 18s rRNA, ammonium, nitrite
Opis:
A new peritrich species highly tolerant to ammonium and nitrite, Epistylis camprubii n. sp., was found adhered to the biofilm of two advanced wastewater treatment plants treating high ammonium-loaded wastewater in Rubí, Spain. Its morphology, oral infraciliature and phylogenetic position in the peritrich clade were studied. The new species is a vase-shaped peritrich, constricted below the peristomial lip, with an in vivo average length of 58.7 ± 10.1 µm, average width of 32.0 ± 5.4 µm, and a longitudinally striated, compact stalk that occasionally exhibits uneven thickness and rarely shows transverse segments. The peristomial disc is commonly rounded or pointed, and rarely umbilicated. The C-shaped macronucleus is located in the adoral half of the body, and the only contractile vacuole lies in the adoral third of the zooid. The molecular analysis of the 18s gene sequence clustered E. camprubii n. sp. together with the other Epistylis, with the exception of Epistylis galea.
Źródło:
Acta Protozoologica; 2016, 55, 1
1689-0027
Pojawia się w:
Acta Protozoologica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
EF1α is a suitable housekeeping gene for RT-qPCR analysis during osteogenic differentiation of mouse bone marrow-derived mesenchymal stem cells
Autorzy:
Chen, Xingyun
Zhang, Bo
Zhao, Yan
Liu, Ping
Zhou, Yuanguo
Powiązania:
https://bibliotekanauki.pl/articles/1039535.pdf
Data publikacji:
2013
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
EF1α; RGS4; 18S rRNA; RT-qPCR; RPL 13a; CCG-1986
Opis:
The expression of predominant housekeeping genes used in RT-qPCR can vary during development and differentiation. The frequently used housekeeping genes (ACTB, GAPDH, 18S rRNA, EF1α and RPL 13a) were evaluated during an early stage of the osteogenic differentiation of mouse bone marrow-derived mesenchymal stem cells (mMSCs) (under normal conditions or treated with CCG-4986) to identify housekeeping genes whose expression remained constant during osteogenic differentiation. When we used RGS4 mRNA, which was determined as copy number per μg of total RNA, to normalize gene expression, we observed that the relative EF1α expression profile was consistent with RGS4 expression after treatment with CCG-4986. All the relative expression profiles of the EF1α, 18S rRNA, and RPL13a housekeeping genes were consistent with RGS4 profiles determined by measuring mRNA copies under normal osteogenic differentiation conditions. The expression profiles calibrated by ACTB and GAPDH were not consistent with those determined using mRNA copy number in untreated cells or cells treated with CCG-4986 under osteogenic differentiation conditions. Under normal osteogenic differentiation conditions, EF1α, 18S rRNA, and RPL 13a are suitable housekeeping genes for RT-qPCR analysis. However, EF1α is the only suitable gene upon CCG-4986 treatment.
Źródło:
Acta Biochimica Polonica; 2013, 60, 3; 381-386
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Taxonomic Descriptions of Two Marine Ciliates, Euplotes dammamensis n. sp. and Euplotes balteatus (Dujardin, 1841) Kahl, 1932 (Ciliophora, Spirotrichea, Euplotida), Collected from the Arabian Gulf, Saudi Arabia
Autorzy:
Chen, Xumiao
Zhao, Yan
Al-Farraj, Saleh A.
AL-QURAISHY, Saleh
El-Serehy, Hamed A.
Shao, Chen
Al-Rasheid, Khaled A. S.
Powiązania:
https://bibliotekanauki.pl/articles/763475.pdf
Data publikacji:
2013
Wydawca:
Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
Tematy:
Marine ciliate, morphogenesis, morphology, new species, SSU-rRNA, taxonomy
Opis:
The morphology, morphogenesis and infraciliature of two marine euplotid ciliates, Euplotes dammamensis n. sp. and Euplotes balteatus (Dujardin, 1841) Kahl, 1932, isolated from a sandy beach of the Arabian Gulf, Saudi Arabia, were investigated using observations in vivo and protargol-impregnation methods. Euplotes dammamensis n. sp. is characterized by a combination of features including its huge body size (100–170 × 80–120 μm), 10 conspicuous dorsal ridges, 10 normal-sized frontoventral and two marginal cirri, and 11 dorsal kineties. Euplotes balteatus is mainly characterized by 10 frontoventral, two caudal, and two left marginal cirri, 7–10 dorsal kineties and 5–7 prominent dorsal ridges as well as double-eurystomus silverline system. The small subunit rRNA (SSU-rRNA) gene sequences were determined for both species and phylogenetic analyses based on these data indicated that E. dammamensis is most closely related to E. parabalteatus Jiang et al., 2010, and E. balteatus clusters with E. plicatum Valbonesi et al., 1997, E. orientalis Jiang et al., 2010, and E. bisulcatus Kahl, 1932.
Źródło:
Acta Protozoologica; 2013, 52, 2
1689-0027
Pojawia się w:
Acta Protozoologica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prevalence of Anaplasma phagocytophilum in Ixodes ricinus ticks determined by polymerase chain reaction with two pairs of primers detecting 16S rRNA and ankA genes
Autorzy:
Chmielewska-Badora, J
Zwolinski, J.
Cisak, E.
Wojcik-Fatla, A.
Buczek, A.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/51023.pdf
Data publikacji:
2007
Wydawca:
Instytut Medycyny Wsi
Tematy:
Lublin region
Polska
pathogen
Ixodes ricinus
Eastern Poland
tick
determination
animal pathogen
human pathogen
ankA gene
16S rRNA gene
granulocytic anaplasmosis
bacteria
Anaplasma phagocytophilum
polymerase chain reaction
Opis:
A total of 684 Ixodes ricinus ticks (321 nymphs, 184 males, and 179 females) were collected by fl agging lower vegetation in 6 forest districts located on the territory of Lublin province (eastern Poland). Ticks were examined by polymerase chain reaction (PCR) method for the presence of Anaplasma phagocytophilum DNA with two pairs of primers: EHR521/EHR747 for detecting 16S rRNA gene, and LA6/LA1 for detecting ankA gene. To study the relationship between infection in ticks and people occupationally exposed to tick bite, blood serum samples of 261 forestry workers employed in the same forest districts were examined by immunofl uorescence method for the presence of specifi c antibodies against A. phagocytophilum. A total of 70 ticks out of 684 examined (10.2%) showed the presence of A. phagocytophilum 16S rRNA gene. The prevalence of infection was signifi cantly dependent on tick’s stage (χ2=49.2, p<0.00001) and geographical locality (χ2=34.4, p<0.00001). The percentage of I. ricinus females infected with A. phagocytophilum (24.6%) was signifi cantly greater compared to males (6.5%) and nymphs (4.4%) (p<0.00001). Only 19 ticks out of 684 examined (2.8%) showed the presence of A. phagocytophilum ankA gene, signifi cantly less compared to 16S rRNA gene (p<0.00001). The prevalence of infection demonstrated by the presence of ankA gene was also signifi cantly dependent on tick’s stage (χ2=23.6, p<0.00001) but not on locality (χ2=9.8, p=0.082). A signifi cant correlation was found between the presence of A. phagocytophilum 16S rRNA gene in I. ricinus female ticks from the particular forest districts and the serologic response to A. phagocytophilum of forestry workers employed in the same districts (p<0.05). No signifi cant correlation was found between the presence of A. phagocytophilum ankA gene in I. ricinus ticks and serologic response of exposed workers. In conclusion, detection of A. phagocytophilum infection in ticks by PCR with the use of EHR521/EHR747 primers detecting 16S rRNA gene is signifi cantly more sensitive compared to LA6/LA1 primers detecting ankA gene.
Źródło:
Annals of Agricultural and Environmental Medicine; 2007, 14, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wykorzystanie sekwencji genu rRNA w molekularnej diagnostyce parazytologicznej
Molecular diagnostic of parasites using rRNA gene sequence
Autorzy:
Dlugosz, E
Wisniewski, M.
Powiązania:
https://bibliotekanauki.pl/articles/841440.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
geny kodujace
diagnostyka parazytologiczna
diagnostyka weterynaryjna
wykorzystanie
parazytologia
konferencje
sekwencja genu
gen rRNA
Warszawa konferencja
Opis:
Ribosomal RNA (rRNA) is a component of the ribosomes. Eukaryotic ribosomes contain four different rRNA molecules: 18S, 5,8S, 28S and 5S rRNA. rRNA is the most conserved (least variable) gene in all cells. For this reason, genes that encode the rRNA (rDNA) are sequenced to identify an organism's taxonomic group, calculate related groups, and estimate rates of species divergence. Especially the internal transcribed spacers (ITS) are very useful for molecular diagnostic of parasite. They are noncoding regions of DNA sequence that separate genes coding for the 28S, 5.8S, and 18S ribosomal RNAs. These ribosomal RNA (rRNA) genes are highly conserved across taxa while the spacers between them may be species-specific. In this paper authors describe practical using of rRNA gene to parasite diagnostic.
Źródło:
Annals of Parasitology; 2006, 52, 4; 263-269
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wykorzystanie sekwencji genu rRNA w molekularnej diagnostyce parazytologicznej
Molecular diagnostic of parasites using rRNA gene sequence
Autorzy:
Długosz, E.
Wiśniewski, M.
Powiązania:
https://bibliotekanauki.pl/articles/2144424.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
geny kodujace
diagnostyka parazytologiczna
diagnostyka weterynaryjna
wykorzystanie
parazytologia
konferencje
sekwencja genu
gen rRNA
Warszawa konferencja
Opis:
Ribosomal RNA (rRNA) is a component of the ribosomes. Eukaryotic ribosomes contain four different rRNA molecules: 18S, 5,8S, 28S and 5S rRNA. rRNA is the most conserved (least variable) gene in all cells. For this reason, genes that encode the rRNA (rDNA) are sequenced to identify an organism's taxonomic group, calculate related groups, and estimate rates of species divergence. Especially the internal transcribed spacers (ITS) are very useful for molecular diagnostic of parasite. They are noncoding regions of DNA sequence that separate genes coding for the 28S, 5.8S, and 18S ribosomal RNAs. These ribosomal RNA (rRNA) genes are highly conserved across taxa while the spacers between them may be species-specific. In this paper authors describe practical using of rRNA gene to parasite diagnostic.
Źródło:
Wiadomości Parazytologiczne; 2006, 52, 4; 263-269
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Monitoring the occurrence of bacteria in stored cabbage heads
Autorzy:
Eichmeier, Aleš
Peňázová, Eliška
Pečenka, Jakub
Čechová, Jana
Pokluda, Robert
Tekielska, Dorota
Baránek, Miroslav
Powiązania:
https://bibliotekanauki.pl/articles/961651.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bacteria
cabbage
identifi cation
isolation
mpa medium
pseudomonas
sequencing
16s rrna
Bacillus
Opis:
Twenty-six cabbage heads stored under typical conditions in a storage hall in Moravia, Czech Republic, were tested for the presence of bacteria by the method of isolation from three different parts of the cabbage heads. Isolations were carried out from stalks, inner and superficial leaves. Two samplings were done; in November 2015 and February 2016. Bacterial cultures were sequenced in the part of 16S rRNA region; bacteria were identified according to the sequences obtained. The most prevalent bacteria were of the genus Pseudomonas. Genera: Klebsiella, Erwinia, Pantoea, Bacillus were also identified. The results provided an interesting insight into the bacterial spectrum in stored cabbage heads and their dynamics during storage. Th e nucleotide sequences which were found were saved in GenBank/NCBI under accession numbers KX160104-KX160145.
Źródło:
Journal of Plant Protection Research; 2017, 57, 1
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genetic characterization of some rhizobial isolates from various legumes
Autorzy:
El-Ghany, E.M.A.
Eissa, R.A.
Fahmi, A.I.
Nagaty, H.H.
El-Zanaty, A.M.
Powiązania:
https://bibliotekanauki.pl/articles/2097126.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
rhizobia
morphological
API 20E and API 20NE
16S rRNA
phylogenetic
Opis:
In the last few decades, there has been a growing interest in environmentally friendly sustainable agricultural practices, thus increasing the role of biofertilizers such as rhizobia, which can decrease the need for chemical fertilizers, reduce adverse environmental effects, and help to save money. Therefore, information on the distribution and genetic variation of native rhizobial isolates would aid in selecting novel rhizobial strains that could be developed and used as biofertilizers in legume production. This research was conducted to characterize 24 rhizobial isolates from five legumes on morphological, biochemical, and molecular aspects and determine the phylogenetic relationships among them. Rhizobial isolates were obtained from five Egyptian legumes: faba bean, lentil, pea, clover, and soybean. Morphological characterization classified the isolates into fast and slow growers. Biochemical characterization using API 20E and API 20NE systems showed a large diversity, which may reflect their adaptation in different environments. Moreover, molecular detection of the 16S rRNA gene enabled to characterize 19 of them to the species level. Rhizobial isolates from pea, faba bean, clover, and lentil were identified as Rhizobium leguminosarum and those from soybean were identified as Bradyrhizobium japonicum. These data reflected a narrow diversity of rhizobial species in Egypt. A phylogenetic analysis of the 19 isolates confirmed that B. japonicum isolates were divergent from all other isolates. Furthermore, the phylogram revealed that each group of isolates that originated from the root nodule of a certain legume formed a separate subcluster. The obtained data suggested a narrow range of interspecies variations, which is consistent with the idea of the presence of biovars among the species.
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2020, 101, 3; 179-191
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The ribosome filter hypothesis and specialized ribosomes
Autorzy:
Filipek, Kamil
Deryło, Kamil
Michalec-Wawiórka, Barbara
Powiązania:
https://bibliotekanauki.pl/articles/1178213.pdf
Data publikacji:
2018
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
filter hypothesis
rRNA
ribosomal proteins
ribosome
specialized ribosomes
Opis:
The ribosome is a macromolecular complex of proteins and RNA, which plays a key role in every living organism, being a heart of the process of translation. Recent findings have shown that it can be also regarded as a regulatory element that adjusts cellular proteome to highly variable environmental conditions. The ribosome is believed to possess the ability to “filter” populations of mRNAs for choosing their appropriate set to meet current demands of the cell. The filter mechanism is based on a specific interaction between mRNA and rRNA or mRNA and ribosomal proteins. The ribosome “filtering activity” is reflected by the ribosomal particles heterogeneity, which originates mainly from variations or modifications within particular components of translational apparatus. Alternations of ribosomal proteins or/and rRNA generate a specific class of ribosomes called specialized ribosomes, which having unique composition can display selectivity toward particular mRNAs representing an additional step of gene expression regulation at the translational level. This work describes a basis of ribosome filter hypothesis illustrated by interesting examples from different domains of life.
Źródło:
World Scientific News; 2018, 93; 19-29
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Detection and identification of potentially toxic cyanobacteria in Polish water bodies
Autorzy:
Głowacka, Joanna
Szefel-Markowska, Magdalena
Waleron, Małgorzata
Łojkowska, Ewa
Waleron, Krzysztof
Powiązania:
https://bibliotekanauki.pl/articles/1039881.pdf
Data publikacji:
2011
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
mcyB
microcystins
toxins
16S rRNA
Cyanobacteria
mcyE
rpoC1
Opis:
The main goal of this study was to determine the distribution of potentially toxic cyanobacteria in 39 selected Polish water bodies. From the water bodies with blooms and also from those in which blooms were not visible 87 samples were investigated. For the first time samples from ponds localized in villages with high agricultural activities were included. Lakes for which microcystin concentrations had been determined before were included as a reference for the research. The detection of cyanobacteria was conducted by microscopic observation as well as by PCR amplification of the rpoC1 gene fragment. Cyanobacteria were present in 75 out of 87 samples. The presence of potentially toxic cyanobacteria was detected by amplification of the mcyB and mcyE genes, which are involved in the biosynthesis of microcystins. Both genes were detected in 7 out of 9 blooms investigated. In the case of samples collected from water bodies in which blooms were not observed, the mcyB and mcyE genes were detected in 20 out of 36. In order to identify the cyanobacteria occurring in selected reservoirs, 16S plus ITS clone libraries were constructed. The method allowed distinguishing 18 different genotypes. After sequence analysis, cyanobacteria belonging to genera Microcystis, Planktothrix, Anabaena, Pseudanabaena, Synechocystis, Synechococcus and Woronichinia were identified. Results confirmed the usefulness of the rpoC1 and mcy genes for monitoring water bodies and detection of potentially toxic cyanobacteria. Application of molecular markers allowed detecting potentially toxic cyanobacteria before the bloom was visible. This is the first comprehensive study concerning cyanobacteria present in different types of Polish water bodies performed using molecular markers.
Źródło:
Acta Biochimica Polonica; 2011, 58, 3; 321-333
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Diagnosis of Pneumocystis carinii infection by a serum polymerase chain reaction
Autorzy:
Golab, E.
Sobolewska, A.
Dzbenski, T.
Bitkowska, E.
Powiązania:
https://bibliotekanauki.pl/articles/836353.pdf
Data publikacji:
1998
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
Pneumocystis carinii
infection
serum
diagnosis
laboratory diagnosis
pneumonia
polymerase chain reaction
rRNA gene
T cell
Źródło:
Annals of Parasitology; 1998, 44, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 48

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