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Wyszukujesz frazę "rDNA sequence analysis" wg kryterium: Temat


Wyświetlanie 1-3 z 3
Tytuł:
Heterotrophic bacteria from brackish water of the Southern Baltic Sea: biochemical and molecular identification and characterisation
Autorzy:
Cabaj, A.
Palinska, K.
Kosakowska, A.
Kurlenda, J.
Powiązania:
https://bibliotekanauki.pl/articles/48857.pdf
Data publikacji:
2006
Wydawca:
Polska Akademia Nauk. Instytut Oceanologii PAN
Tematy:
molecular identification
surface water
biochemical identification
rDNA sequence analysis
Baltic Sea
Bacillaceae
brackish water
heterotrophic bacteria
Opis:
Six bacterial strains isolated from the surface water of the southern Baltic Sea were described on the basis of their morphological, physiological and biochemical features, and were classified on the basis of 16S rDNA sequence analysis. Comparative analyses of the 16S rDNA sequences of five of the six bacterial strains examined displayed a ≥98% similarity to the sequences available in the NCBI GenBank. The 16S rDNA sequence of strain 2 shared only a 96% similarity with other published sequences, which suggests that this is a new, hitherto unknown species. The isolated heterotrophic bacteria belong to the families Bacillaceae (strain 1), Flexibacteriaceae (strain 2), Sphingomonadaceae (strains 3, 5), Micrococcaceae (strain 4) and Aurantimonadaceae (strain 6). This is the first study in which the polyphasic approach has been applied to the identification of heterotrophic bacteria from the brackish waters of the Gulf of Gdańsk and Gdańsk Deep.
Źródło:
Oceanologia; 2006, 48, 4
0078-3234
Pojawia się w:
Oceanologia
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparative cytogenetic analysis of diploid and hexaploid Chenopodium album Agg.
Autorzy:
Kolano, B
Siwinska, D.
Maluszynska, J.
Powiązania:
https://bibliotekanauki.pl/articles/56512.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
cytogenetic analysis
diploid
hexaploid
Chenopodium album
genome size
rDNA sequence
flow cytometry
polysomaty
Opis:
Two cytotypes of Chenopodium album, diploid (2n=2x=18) and hexaploid (2n=6x=54), were analysed using flow cytometry and a FISH experiment. The genome size was indicated as 1.795 pg for the diploid and 3.845 pg for the hexaploid plants which suggested genome downsizing in the evolution of hexaploid cytotype. Double FISH with 25S rDNA and 5S rDNA allowed three to five homologue chromosome pairs to be distinguished depending on the cytotype. The Variation in size and number of rDNA sites between the polyploid C. album and its putative diploid ancestor indicated that rDNA loci underwent rearrangements after polyploidization. Flow cytometry measurements of the relative nuclear DNA content in the somatic tissue of C. album revealed extensive endopolyploidization resulting in tissues comprising a mixture of cells with a different DNA content (from 2C to 32C) in varying proportions. The pattern of endopolyploidy was characteristic for the developmental stage of the plant and for the individual organ. Polysomaty was not observed in the embryo tissues however endopolyploidization had taken place in most tested organs of seedlings. The endopolyploidy in diploid and hexaploid C. album was compared to find any relationship between the pattern of polysomaty and polyploidy level in this species. This revealed that polyploid plants showed a decline in the number of endocycles as well as in the frequency of endopolyploidy cells compared to diploid plants.
Źródło:
Acta Societatis Botanicorum Poloniae; 2008, 77, 4; 293-298
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Characterization of cereal cyst nematodes (Heterodera spp.) in Morocco based on morphology, morphometrics and rDNA-ITS sequence analysis
Autorzy:
Mokrini, F.
Viaene, N.
Waeyenberge, L.
Dababat, A.A.
Moens, M.
Powiązania:
https://bibliotekanauki.pl/articles/65431.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
cereal cyst nematode
nematode
Heterodera avenae
Heterodera latipons
Heterodera filipjevi
morphology
morphometry
Internal transcribed spacer
rDNA
sequence analysis
Morocco
Opis:
Morphological and molecular diversity among 11 populations of cereal cyst nematodes from different wheat production areas in Morocco was investigated using light microscopy, species-specific primers, complemented by the ITS-rDNA sequences. Morphometrics of cysts and second-stage juveniles (J2s) were generally within the expected ranges for Heterodera avenae; only the isolate from Aïn Jmaa showed morphometrics conforming to those of H. latipons. When using species-specific primers for H. avenae and H. latipons, the specific bands of 109 bp and 204 bp, respectively, confirmed the morphological identification. In addition, the internal transcribed spacer (ITS) regions were sequenced to study the diversity of the 11 populations. These sequences were compared with those of Heterodera species available in the GenBank database (www.ncbi.nlm.nih.gov) and confirmed again the identity of the species. Ten sequences of the ITS-rDNA were similar (99–100%) to the sequences of H. avenae published in GenBank and three sequences, corresponding with one population, were similar (97–99%) to H. latipons.
Źródło:
Journal of Plant Protection Research; 2017, 57, 3
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-3 z 3

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