Temat: qPCR - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: DNA vs RNA based studies of nitrogen removal bacteria genes via qPCR
DNA i RNA-zależna analiza genów bakterii przemian azotowych metodą qPCR
Autorzy:: Banach-Wiśniewska, Anna
Gamoń, Filip
Ziembińska-Buczyńska, Aleksandra
Powiązania:: https://bibliotekanauki.pl/articles/1845429.pdf
Data publikacji:: 2021
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: qPCR
RT-qPCR
nitrogen removal bacteria
relative gene abundance
bakterie usuwające azot
względna obfitość genów
Opis:: Improvements in water quality requires the removal of nitrogen compounds from wastewater. The most promising and cost-effective methods for this purpose are biological ones based on activated sludge microorganisms such as nitrifiers, denitrifiers, and anammox bacteria. Due to the most of the nitrogen removal bacteria are uncultivable in a laboratory, the application of the molecular tools is required to investigate microorganisms involved in the nitrogen removal. In case of this study for the analysis of relative genes abundance of nitrogen removal bacteria, quantitative PCR (qPCR) based on bacterial DNA and qPCR preceded by reverse transcription (RT-qPCR) based on bacterial mRNA as a template, were used with specific bacterial functional genes (amoA, nrxA, nirS, nirK, hzo). Samples from four anammox sequencing batch reactors (SBRs) were analyzed, while the nitrogen removal process and bacteria growth were supported by biomass immobilization and nanoparticles addition. There were statistically significant differences between results obtained in the case of mRNA and DNA (p<0.05). Statistically significant positive correlations were found between results obtained with those two approaches. In case of mRNA analysis, positive results were obtained only for hzo, amoA and partly for nirS genes, despite additional purification and removal of inhibitors from samples prior to reaction.
Z uwagi na to, że większości bakterii przemian związków azotowych nie można wyizolować w postaci czystych kultur, do ich zbadania konieczne jest zastosowanie metod biologii molekularnej. Jedną z najczęściej stosowanych w tym celu jest ilościowa reakcja łańcuchowa polimerazy (ang. Quantitative Polimerase Chain Reaction, qPCR). Celem eksperymentu było porównanie wyników analizy wybranych genów funkcyjnych bakterii przemian związków azotowych przy pomocy metody qPCR wykonanej na matrycy DNA i RNA (po odwrotnej transkrypcji). Względna liczebności genów funkcyjnych analizowana była z zastosowaniem metody qPCR (na matrycy DNA) oraz RT-qPCR (ang. Reverse Transcription-qPCR) (na matrycy RNA). Analizę przeprowadzono w oparciu o geny: amoA, nrxA, nirS, nirK i hzo. Próbki osadu czynnego pobrano z czterech sekwencyjnych reaktorów porcjowych, w których proces usuwania azotu i wzrostu bakterii wspomagano za pomocą immobilizacji biomasy i dodatkiem nanocząstek. Wykazano statystycznie istotne różnice między wynikami uzyskanymi w przypadku badań mRNA i badań opartych na DNA (p<0,05). Wyniki uzyskane za pomocą zastosowanych narzędzi biologii molekularnej (qPCR, RT-qPCR) były skorelowane pozytywnie. W przypadku analizy opartej na mRNA pozytywne wyniki uzyskano tylko dla genów hzo, amoA i częściowo dla genów nirS, pomimo dodatkowego oczyszczania i usuwania inhibitorów z próbek przed reakcją. Należy podkreślić, że w zależności od matrycy zastosowanej w qPCR (bakteryjne DNA lub cDNA zsyntetyzowane z bakteryjnego mRNA w procesie odwrotnej transkrypcji) uzyskane wyniki mogą wskazywać na różne informacje naukowe. Pomimo znaczących różnic pomiędzy wynikami otrzymanymi za pomocą dwóch metod, obliczone współczynniki korelacji Spearmana wskazują na wzajemne powiązanie pomiędzy otrzymanymi wynikami oraz powiązania ekologiczne pomiędzy bakteryjnymi genami przemian związków azotowych.
Źródło:: Archives of Environmental Protection; 2021, 47, 1; 19-25
2083-4772
2083-4810
Pojawia się w:: Archives of Environmental Protection
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Feeding state and age dependent changes in melanin-concentrating hormone expression in the hypothalamus of broiler chickens
Autorzy:: Simon, Ádám
Németh, József
Jávor, András
Komlósi, István
Bai, Péter
Oláh, János
Juhász, Béla
Kiss, Rita
Szilvássy, Zoltán
Czeglédi, Levente
Powiązania:: https://bibliotekanauki.pl/articles/1038398.pdf
Data publikacji:: 2018
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: chicken
feeding states
hypothalamus
MCH
qPCR
RIA
Opis:: We aimed to quantify the gene expression changes of the potent orexigenic melanin-concentrating hormone (MCH) in chicken (Gallus gallus) hypothalamus with quantitative real-time polymerase chain reaction (qPCR), and for the first time determine peptide concentrations with a novel radioimmunoassay (RIA) under different feeding status. Three different experimental conditions, namely ad libitum feeding; fasting for 24 h; fasting for 24 h and then refeeding for 2 h, were applied to study changes of the aforementioned target and its receptor (MCHR4) gene expression under different nutritional status. The relative changes of MCH and MCHR4 were also studied from 7 to 35 days of age. Expression of PMCH and MCHR4 along the gastrointestinal tract (GIT) was also investigated. We found that expression of both targets was significant in the hypothalamus, while only weak expression was detected along the GIT. Different nutritional states did not affect the PMCH and MCHR4 mRNA levels. However, fasting for 24 h had significantly increased the MCH-like immunoreactivity by 25.65%. Fasting for 24 h and then refeeding for 2 h had further significantly increased the MCH peptide concentration by 32.51%, as compared to the ad libitum state. A decreasing trend with age was observable for both, the PMCH and MCHR4 mRNA levels, and also for the MCH-like immunoreactivity. Correlation analysis did not result in a significant correlation between MCH peptide concentration and abdominal fat mass in ad libitum fed birds. In conclusion, MCH peptide concentration altered in response to 24 h fasting, which indicated that this peptide may take part in feed intake regulation of broiler chickens.
Źródło:: Acta Biochimica Polonica; 2018, 65, 2; 251-258
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Characterization of conditions and determination of practical tips for mtDNA level estimation in various human cells
Autorzy:: Jędrak, Paulina
Sowa, Natalia
Barańska, Sylwia
Węgrzyn, Grzegorz
Powiązania:: https://bibliotekanauki.pl/articles/1038562.pdf
Data publikacji:: 2017
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: mtDNA level
leukocytes
fibroblasts
qPCR methodology
Huntington disease
Opis:: Determination of mtDNA copy number in the cell is crucial to understand many cellular processes. Recently, the number of studies with the use of mitochondrial DNA (mtDNA) content as the determinant of mitochondrial abnormalities increased greatly and is still growing, therefore, optimization of technical conditions for this analysis is crucial. Despite using similar laboratory protocols, some results cannot be compared between research centers, thus causing discrepancies in the assessment of mtDNA content. The aim of this work was to test which conditions of biological sample collection and storage affect estimation of mtDNA level relative to the nuclear DNA (nDNA) in the blood samples and dermal fibroblasts. We found that the time and temperature of sample storage, as well as the type of the blood sample (whole blood or leukocytes) influence the estimate of mtDNA/nDNA ratio in the blood. In the case of dermal fibroblasts collected from healthy control and Huntington disease patients, our data indicate that the passage number of cells is essential to obtain reliable results.
Źródło:: Acta Biochimica Polonica; 2017, 64, 4; 699-704
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: The sequence diversity and expression among genes of the folic acid biosynthesis pathway in industrial Saccharomyces strains
Autorzy:: Goncerzewicz, Anna
Misiewicz, Anna
Powiązania:: https://bibliotekanauki.pl/articles/1038930.pdf
Data publikacji:: 2015
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: folic acid
Saccharomyces cerevisiae
gene polymorphisms
RT qPCR
Opis:: Folic acid is an important vitamin in human nutrition and its deficiency in pregnant women's diets results in neural tube defects and other neurological damage to the fetus. Additionally, DNA synthesis, cell division and intestinal absorption are inhibited in case of adults. Since this discovery, governments and health organizations worldwide have made recommendations concerning folic acid supplementation of food for women planning to become pregnant. In many countries this has led to the introduction of fortifications, where synthetic folic acid is added to flour. It is known that Saccharomyces strains (brewing and bakers' yeast) are one of the main producers of folic acid and they can be used as a natural source of this vitamin. Proper selection of the most efficient strains may enhance the folate content in bread, fermented vegetables, dairy products and beer by 100% and may be used in the food industry. The objective of this study was to select the optimal producing yeast strain by determining the differences in nucleotide sequences in the FOL2, FOL3 and DFR1 genes of folic acid biosynthesis pathway. The Multitemperature Single Strand Conformation Polymorphism (MSSCP) method and further nucleotide sequencing for selected strains were applied to indicate SNPs in selected gene fragments. The RT qPCR technique was also applied to examine relative expression of the FOL3 gene. Furthermore, this is the first time ever that industrial yeast strains were analysed regarding genes of the folic acid biosynthesis pathway. It was observed that a correlation exists between the folic acid amount produced by industrial yeast strains and changes in the nucleotide sequence of adequate genes. The most significant changes occur in the DFR1 gene, mostly in the first part, which causes major protein structure modifications in KKP 232, KKP 222 and KKP 277 strains. Our study shows that the large amount of SNP contributes to impairment of the selected enzymes and S. cerevisiae and S. pastorianus produce reduced amounts of the investigated metabolite. The results obtained here yield a list of genetically stable yeast strains which can be implemented as a starter culture in the food industry.
Źródło:: Acta Biochimica Polonica; 2015, 62, 4; 841-850
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: Poszukiwanie regionów DNA sprzężonych z tolerancją wegetatywnych podkładek jabłoni na niskie temperatury poprzez analizę transkryptomu i ocenę stopnia polimorfizmu genów kandydujących
Identification of the genome regions correlated with cold hardiness of apple rootstocks by transcriptomic analysis of differentially expressed candidate genes
Autorzy:: Keller-Przybyłkowicz, Sylwia
Lewandowski, Mariusz
Korbin, Małgorzata
Powiązania:: https://bibliotekanauki.pl/articles/2199558.pdf
Data publikacji:: 2019-11-30
Wydawca:: Instytut Hodowli i Aklimatyzacji Roślin
Tematy:: adnotacja
EST
jabłoń
qPCR
RNAseq
sekwencjonowanie NGS
transkryptom
Źródło:: Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin; 2019, 286; 415-418
0373-7837
2657-8913
Pojawia się w:: Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 6.

Tytuł:: Molecular cloning, expression and characterization of Bmserpin-2 gene from Bombyx mori
Autorzy:: Pan, Ye
Xia, Hengchuan
Lü, Peng
Chen, KePing
Yao, Qin
Chen, Huiqin
Gao, Lu
He, Yuanqing
Wang, Lin
Powiązania:: https://bibliotekanauki.pl/articles/1040486.pdf
Data publikacji:: 2009
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: serpin-2
Bombyx mori
bioinformatics
subcellular location
qPCR
Opis:: Serpins are a broadly distributed family of protease inhibitors. In this study, the gene encoding Bombyx mori serpin-2 (Bmserpin-2) was cloned and expressed in E. coli. The Bmserpin-2 cDNA contains a 1125 bp open reading frame (ORF). The deduced protein has 374 amino-acid residues, contains a conserved SERPIN domain and shares extensive homology with other invertebrate serpins. RT-PCR analysis showed that Bmserpin-2 was expressed in all developmental stages of B. mori larvae and various larval tissues. Subcellular localization analysis indicated that Bmserpin-2 protein was located in the cytoplasm. Interestingly, real-time quantitative PCR revealed that the expression of Bmserpin-2 in the midgut of susceptible B. mori strain 306 significantly increased at 72 hours post inoculation (hpi) when infected with BmNPV. However, there was no significant increase of the Bmserpin-2 expression in resistant strain NB infected with BmNPV. Thus, our data indicates that Bmserpin-2 may be involved in B. mori antiviral response.
Źródło:: Acta Biochimica Polonica; 2009, 56, 4; 671-677
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 7.

Tytuł:: Defense responses of rice plant to Monographella albescens attack
Autorzy:: Almas, D.E.
Kamrodi, A.R.
Powiązania:: https://bibliotekanauki.pl/articles/2084805.pdf
Data publikacji:: 2019
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: defense-signaling genes
enzyme activities
Monographella albescens
rice
RT-qPCR
Opis:: Leaf scald, caused by the necrotrophic fungus Monographella albescens, is one of the main threats to rice (Oryza sativa L.) around the world. This disease decreases yields in rice by up to 30% because of dead leaf tissue, damaged seeds, and sterile flowers. Currently, there is limited knowledge about the molecular mechanisms involved in rice plant resistance against this pathogen. For this purpose, six commercial cultivars of rice were primarily screened for M. albescens infection and development. Dasht and Salari were found to be the most resistant and susceptible to M. albescens infection, respectively. The plants were kept in a greenhouse at 29 ± 2°C during the day and 26 ± 2°C at night with a relative air humidity of 85 ± 5%. Forty-five days after sowing, the plants with three biological replications were inoculated by transferring a PDA disc (0.3 cm2) containing M. albescens mycelia to the middle third of the 7th, 8th, and 9th completely open leaves. The leaves were collected 24, 48, 72, 96 and 120 hai. Leaf samples were also collected from the non-inoculated plants (0 h) to serve as controls. Real-time quantitative PCR (RT-qPCR) showed rapid induction and significant accumulation of jasmonic acid (JA) and ethylene (ET) responsive genes such as lipoxygenase (LOX), allene oxide synthase 2 (Aos2), jasmonic acid carboxyl methyltransferase 1 (JMT1) and ACC synthase 1 (ACS1) in the resistant Dasht cultivar after infection with M. albescens. Furthermore, the transcripts of salicylic acid (SA) responsive phenyl alanine ammonia lyase 1 (PAL1) and nonexpressor of pathogenesis-related genes 1 (NPR1) genes were induced in the incompatible interaction. The activities of the defense enzymes superoxide dismutase (SOD), peroxidase (POX) and glutathione reductase (GR) increased strongly in Dasht in response to M. albescens infection. In addition, there was an increase in the H2 O2 levels in the leaves of the Dasht cultivar during the infectious period of M. albescens associated with the enhancement of catalase (CAT) activity as well as higher levels of malondialdehyde (MDA). This is the first study on the interaction between rice and M. albescens at the molecular level. It can contribute to understanding how rice responds to pathogen infection, as well as assist with future research plans of molecular breeding regarding the tolerance to leaf scald disease.
Źródło:: Journal of Plant Protection Research; 2019, 59, 4; 535-543
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 8.

Tytuł:: Fusarium DNA levels as compared to mycotoxin levels in Finnish and Estonian grain samples.
Autorzy:: Yli-Mattila, T.
Rämö, S.
Tanner, R.
Loiveke, H.
Hietaniemi, V.
Powiązania:: https://bibliotekanauki.pl/articles/2199625.pdf
Data publikacji:: 2011-12-20
Wydawca:: Instytut Hodowli i Aklimatyzacji Roślin
Tematy:: DON
Fusarium. culmorum
F. graminearum
TaqMan qPCR
T-2/HT-2
Opis:: In the present work Fusarium DNA levels of deoxynivalenol (DON)-producing F. graminearum and F. culmorum and T-2/HT-2-producing F. langsethiae and F. sporotrichioides were compared to DON and T-2/HT-2 levels in Finnish (19 oat samples) and Estonian (3 oat, 8 wheat and 9 barley) grain samples of the year 2008. The Finnish oat samples were selected based on high (<870 ppb) DON levels. Some of them also contained high levels of zearalenone (ZEN). The main aim was to find out, which Fusarium species is the main DON producer in oats in Finland. In Estonian grain sam- ples all mycotoxin levels were relatively low and below the EU limits. The highest ZEN levels were found in oat in Satakunta in Finland. This is the first study, in which F. graminearum has been found in Estonian grains. The correlation between F. graminearum DNA and DON levels in the combined Finnish and Estonian oat samples was higly significant (R2 = 0.88). In Finnish samples a significant correlation was also found between F. graminearum DNA and ZEN levels (R2 = 0.42). No correlation was found between F. culmorum DNA and DON levels in the combined Finnish and Estonian oat sam- ples. There was also a significant correlation between the combined T-2 and HT-2 and combined F. langsethiae and F. sporotrichioides levels (R2 = 0.38) in the combined Finnish and Estonian oat samples. According to our results F. graminearum is clearly the main DON producer in Finnish oat.
Źródło:: Plant Breeding and Seed Science; 2011, 64; 131-140
1429-3862
2083-599X
Pojawia się w:: Plant Breeding and Seed Science
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 9.

Tytuł:: Different MACS sorting strategies for the enrichment of Lin– (CD34+ CD45–) hematopoietic progenitor cells: preliminary study
Autorzy:: Vašíček, Jaromír
Baláži, Andrej
Parkányi, Vladimír
Bauer, Miroslav
Powiązania:: https://bibliotekanauki.pl/articles/2183569.pdf
Data publikacji:: 2018-12-31
Wydawca:: Uniwersytet Pedagogiczny im. Komisji Edukacji Narodowej w Krakowie
Tematy:: CD34
CD45
flow cytometry
hematopoietic stem cells
MACS
qPCR
rabbit
Opis:: Magnetic-activated cell sorting (MACS) has become a standard method for the isolation of hematopoietic stem/progenitor cells (HSC/HPC) in human or mouse model using CD34 antibodies. However, at the present there is no useable CD34 antibody that could be successfully used for the selection of rabbit HSC/HPC. Therefore, the aim of this preliminary study was to remove all mature cells (CD45+) from the heterogeneous mixture of rabbit peripheral blood and bone marrow mononuclear cells (PBMCs and BMMCs) in order to enrich these cell populations for the CD34+ cells. Briefly, cells were incubated with a CD45 antibody and proper magnetic microbeads. Three different MACS sorting strategies were used in the experiment that differed mainly in the sample loading rate and the number of used magnetic columns. Control (unsorted) and sorted cells were assessed for the sorting efficiency (% of double positive cells for CD45 and Labelling Check Reagent - LCR) by flow cytometry and for the relative expression of CD34 antigen by qPCR. According to flow cytometry, Depl025 mode showed the best sorting efficiency in terms of the lowest percentages of CD45+LCR+ cells for rabbit PBMCs as well as BMMCs. qPCR analysis confirmed this mode as the best in terms of the relative CD34 expression for rabbit PBMCs. However, higher relative expression of CD34 in BMMCs was obtained by other mode - Posselds. In conclusion, this study demonstrates a possible enrichment of rabbit (CD34+) HSC/HPC by the magnetic depletion of mature hematopoietic (CD45+) cells.
Źródło:: Annales Universitatis Paedagogicae Cracoviensis Studia Naturae; 2018, 3 (suppl.); 83-89
2543-8832
2545-0999
Pojawia się w:: Annales Universitatis Paedagogicae Cracoviensis Studia Naturae
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 10.

Tytuł:: Quantitative Dynamics of Chosen Bacteria Phylla in Wastewater Treatment Plants Excess Sludge After Ozone Treatment
Autorzy:: Jurczyk, Łukasz
Koc-Jurczyk, Justyna
Balawejder, Maciej
Powiązania:: https://bibliotekanauki.pl/articles/123123.pdf
Data publikacji:: 2019
Wydawca:: Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:: ozone treatment
excessive sludge
Real-Time qPCR
sludge microbial community
microbial quantitative dynamics
Opis:: Among the waste produced by municipal wastewater treatment plants, the largest technological problem is caused by the excessive sludge, the management of which consumes almost half of the costs of the entire installation. Regardless of the final disposal route, which may be, for example, reclamation of degraded areas, the the sludge derived from a wastewater treatment plant should be firstly subjected to a series of processes aimed at: reducing its volume, improving selected physical properties, recovering energy, rot preventing (stabilization) or eliminating epidemiological threats (hygenizing). These goals could be implemented using commonly known technologies, including: dewatering, liming, thermoconditioning, methanogenesis or composting. One of the alternative solutions is the use of ozonation of raw excess sludge, which, in principle, may lead to the simultaneous resolution of several above-mentioned problems, such as: drainage properties improvement, increase the bioavailability of organic matter for biogas production, as well as the removal of undesirable microorganisms. Confirmation of the hygenizing effects usually requires arduous microbiological methods; therefore, the fast and reliable tools of molecular biology could prove their usefulness in this case. In this study, the possibility of determining the dynamics of selected groups of microorganisms (including changes in total bacteria amount, Proteobacteria, Bacteroidetes, Firmicutes and Actinobacteria) after sewage sludge ozonation, by using the semi-quantitative RealTime polymerase chain reaction (qPCR), wasinvestigated.
Źródło:: Journal of Ecological Engineering; 2019, 20, 3; 204-213
2299-8993
Pojawia się w:: Journal of Ecological Engineering
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 11.

Tytuł:: Soil microbiomes of reclaimed and abandoned mines of the Yamal region
Autorzy:: Pershina, Elizaveta
Ivanova, Ekaterina
Kimeklis, Anastasia
Zverev, Alexey
Kichko, Arina
Aksenova, Tatiana
Andronov, Evgeny
Abakumov, Evgeny
Powiązania:: https://bibliotekanauki.pl/articles/2041836.pdf
Data publikacji:: 2020
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: Arctic
Yamal Peninsula
microbiome
soil
high-throughput sequencing
16S rRNA
qPCR
mining
reclamation
Źródło:: Polish Polar Research; 2020, 41, 1; 95-114
0138-0338
2081-8262
Pojawia się w:: Polish Polar Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 12.

Tytuł:: EF1α is a suitable housekeeping gene for RT-qPCR analysis during osteogenic differentiation of mouse bone marrow-derived mesenchymal stem cells
Autorzy:: Chen, Xingyun
Zhang, Bo
Zhao, Yan
Liu, Ping
Zhou, Yuanguo
Powiązania:: https://bibliotekanauki.pl/articles/1039535.pdf
Data publikacji:: 2013
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: EF1α; RGS4; 18S rRNA; RT-qPCR; RPL 13a; CCG-1986
Opis:: The expression of predominant housekeeping genes used in RT-qPCR can vary during development and differentiation. The frequently used housekeeping genes (ACTB, GAPDH, 18S rRNA, EF1α and RPL 13a) were evaluated during an early stage of the osteogenic differentiation of mouse bone marrow-derived mesenchymal stem cells (mMSCs) (under normal conditions or treated with CCG-4986) to identify housekeeping genes whose expression remained constant during osteogenic differentiation. When we used RGS4 mRNA, which was determined as copy number per μg of total RNA, to normalize gene expression, we observed that the relative EF1α expression profile was consistent with RGS4 expression after treatment with CCG-4986. All the relative expression profiles of the EF1α, 18S rRNA, and RPL13a housekeeping genes were consistent with RGS4 profiles determined by measuring mRNA copies under normal osteogenic differentiation conditions. The expression profiles calibrated by ACTB and GAPDH were not consistent with those determined using mRNA copy number in untreated cells or cells treated with CCG-4986 under osteogenic differentiation conditions. Under normal osteogenic differentiation conditions, EF1α, 18S rRNA, and RPL 13a are suitable housekeeping genes for RT-qPCR analysis. However, EF1α is the only suitable gene upon CCG-4986 treatment.
Źródło:: Acta Biochimica Polonica; 2013, 60, 3; 381-386
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 13.

Tytuł:: Methods to Study the PVY Population in the Potato
Autorzy:: Yin, Zhimin
Powiązania:: https://bibliotekanauki.pl/articles/2199692.pdf
Data publikacji:: 2017-06-20
Wydawca:: Instytut Hodowli i Aklimatyzacji Roślin
Tematy:: differential potato cultivars
multiplex RT-PCR
PVY strains classification
real-time RT-qPCR
sequencing
tobacco bait plants
Opis:: The PVY population in the potato has been studied continuously using tobacco bait plants in potato fields at Młochów since 1980 at two-year intervals and in potato tuber samples collected from different regions of Poland since 2001 yearly. The paper presents the combined biological, serological and molecular assays for PVY identification and strain classification. Biologically, PVY strains are defined with respect to their ability to elicit hypersensitive resistance (HR) mediated by N genes in differential potato cultivars (King Edward, Desiree and Pentland Ivory) and to symptoms in the tobacco (cultivar Samsun). Serologically, an ELISA assay based on polyclonal or monoclonal cocktail antibodies recognizes all PVY strain types, while the specif-ic monoclonal antibodies help to recognize PVYN or PVYO/PVYC strains. Multiplex RT-PCR, Real-time RT-qPCR and sequencing-based assays are used to define the PVY genome structure. In the Polish population of PVY, the strains PVYO, PVYNTN, PVYN-Wi, PVYZ-NTN and PVYE were identified, while the PVYC strain was not detected.
Źródło:: Plant Breeding and Seed Science; 2017, 75; 71-76
1429-3862
2083-599X
Pojawia się w:: Plant Breeding and Seed Science
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 14.

Tytuł:: Od laboratorium do zdrowego sadzeniaka - raport z realizacji projektu FITOEXPORT w oddziale IHAR-PIB w Boninie
From the laboratory to healthy seed tubers - report on the implementation of the project FITOEXPORT in the Bonin Research Center of IHAR-PIB
Autorzy:: Treder, K.
Kaczmarek, A.
Butrymowicz, J.
Powiązania:: https://bibliotekanauki.pl/articles/2135362.pdf
Data publikacji:: 2021
Wydawca:: Instytut Hodowli i Aklimatyzacji Roślin
Tematy:: ziemniaki
sadzeniaki
certyfikacja
wirusy
wykrywanie wirusow
test RT-qPCR
projekt FITOEXPERT
realizacja projektow
Instytut Hodowli i Aklimatyzacji Roslin Oddzial Bonin
Opis:: Oddział IHAR-PIB w Boninie jest członkiem konsorcjum realizującego projekt FITOEXPORT finanso- wany przez NCBiR w ramach programu GOSPOSTRATEG. Zadanie wykonywane w Boninie ma na celu opracowanie i wdrożenie w laboratoriach PIORiN multipleksowego testu RT-PCR w czasie rze- czywistym do wykrywania wirusów Y, L, M, S, A, X oraz wiroida PSTVd. Taki test opracowano w fazie badawczej projektu, a obecnie trwają prace nad jego adaptacją do wykrywania patogenów na masową skalę w celu usprawnienia urzędowej certyfikacji sadzeniaków ziemniaka przez PIORiN.
Źródło:: Ziemniak Polski; 2021, 31, 3; 3-9
1425-4263
Pojawia się w:: Ziemniak Polski
Dostawca treści:: Biblioteka Nauki