- Tytuł:
- An approach based on diffusion to study ligand-macromolecule interaction.
- Autorzy:
-
Housaindokht, Mohammad
Bahrololoom, Mahmood
Tarighatpoor, Shirin
Mossavi-Movahedi, Ali - Powiązania:
- https://bibliotekanauki.pl/articles/1043738.pdf
- Data publikacji:
- 2002
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
binding isotherm
methyl orange
ligand-protein interaction
hen egg-white lysozyme - Opis:
- A new approach has been developed to study binding of a ligand to a macromolecule based on the diffusion process. In terms of the Fick's first law, the concentration of free ligand in the presence of a protein can be determined by the measurement of those ligands which are diffused out. This method is applied to the study of binding of methyl-orange to lysozyme in phosphate buffer of pH 6.2, at 30°C. The binding isotherm was determined initially, followed by application of the Hill equation to the data obtained, then binding constant and binding capacity were estimated.
- Źródło:
-
Acta Biochimica Polonica; 2002, 49, 3; 703-707
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł