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Wyświetlanie 1-15 z 53
Tytuł:
Implication of early nitric oxide and reactive oxygen species generation in potato resistance to Phytophthora infestans
Autorzy:
Abramowski, D.
Arasimowicz-Jelonek, M.
Floryszak-Wieczorek, J.
Powiązania:
https://bibliotekanauki.pl/articles/80727.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
nitric oxide
reactive oxygen species
potato resistance
Phytophthora infestans
reactive nitrogen species
redox signalling
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Increasing the efficiency of potato breeding through marker assisted selection - general thoughts. Molecular markers for late blight resistance - when applied for breeders?
Autorzy:
Trognitz, Bodo R.
Trognitz, Friederike Ch.
Powiązania:
https://bibliotekanauki.pl/articles/2198962.pdf
Data publikacji:
2004-12-20
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
marker assisted selection
potato breeding
potato late blight resistance
Opis:
Despite many breathtaking breakthroughs in the area of crop genetics and genomics, plant breeding still widely depends on the methods that had been worked out almost a century ago. This is not because commercial plant breeders are overly conservative but because the new knowledge lacks efficient and economical tools that would permit their application in practice. Breeders desire supporting technologies that would facilitate laborious and time-consuming screening in the field and laboratory. In particular, resistance screening often cannot be performed satisfactorily as the necessary disease pressure and appropriate pathogen populations may be unavailable. In potato breeding, specific and often complex resistances need to be developed, at the same time maintaining high levels of quality and culinary characteristics. Therefore, it is worthwhile to revisit the facts that comprise the progress in genetics of disease resistance and to analyze current technologies of genotyping and marker assisted selection, with the objective to detect those parameters that limit the efficiency of methods for commercial application. Selection in potato for resistance to late blight will be highlighted as an example. Maps, genes and markers for resistance have been identified – how universal are they? Single genes and quantitative trait loci for race-specific and race non-specific resistance are known – how efficient is their use? Marker technologies based on polymerase chain reaction and DNA hybridization have been developed that are far more efficient than first-generation technologies – is their use in commercial breeding economical? By discussing these issues concepts will emerge that help to pave the way for marker assisted selection (MAS) in potato breeding. The most important parameters required for economical MAS include to have a clear idea of the traits to be selected for, to use proven, reliable markers, to have in place a robust system for the collection and management of DNA samples, and to use technologies whose total cost is below or equal to the cost of the conventional methods. The most striking advantages of MAS are that a breeder will obtain more information than by conventional methodology, the information will be more precise, field labour can be saved and in that way the breeding process will be intensified. The implementation of the new technology could lead to even closer collaboration of breeders and scientists. Possible disadvantages include the relative increase of laboratory and computer work within the breeding program, and possibly higher costs during the implementation phase of the new technology.
Źródło:
Plant Breeding and Seed Science; 2004, 50; 95-105
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Resistance to Phytophthora infestans in diploid and tetraploid potato families. 2. Resistance in tubers
Autorzy:
Swiezynski, K M
Flis, B
Osiecka, M
Sieczka, M T
Zarzycka, H
Powiązania:
https://bibliotekanauki.pl/articles/2046604.pdf
Data publikacji:
1997
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Phytophthora infestans
tetraploid potato
genotype
resistance
potato
tuber
diploid potato
Opis:
The tuber reaction to Phytophthora infestans was evaluated in individual genotypes of four diploid and four tetraploid potato families, their parents and standard cultivars. The genotypes were tested several times. Two testing methods were used, based on evaluation of tuber slices or whole tubers, respectively. All inoculations were done with MP 245, a fungus isolate with a broad virulence spectrum. If the reaction of tuber slices was evaluated, the distribution of genotypes in several families deviated considerably from a normal one. In some distributions more than one peak was detectable, indicating segregation of major genes. Genotypes transgressive in resistance were found in four families. If the reaction of whole tubers was evaluated, the distribution of genotypes did not deviate significantly from a normal one. Genotypes transgressive in resistance were found in one family. The tuber reaction to P. infestans depended to a large extent on testing conditions.
Źródło:
Journal of Applied Genetics; 1997, 38, 1; 33-44
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Bottle necks in breeding late blight resistant potato
Autorzy:
Zimnoch-Guzowska, Ewa
Tatarowska, Beata
Powiązania:
https://bibliotekanauki.pl/articles/2198951.pdf
Data publikacji:
2004-12-20
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
limitations
Phytophthora infestans
potato
resistance breeding
Opis:
The resistance to late blight (LB) is considered as a factor of major importance among resistances to potato pathogens. For four last decades more work has been done on potato resistant to Phytophthora infestans than on breeding for resistance to any other potato disease. Many sources of resistance have been known to breeders for many decades, but the results of their utilization are still disappointing. The difficulties in breeding for LB resistance were assessed by 39 participants of the survey organized for the Global Initiative on Late Blight (Zimnoch-Guzowska and Flis 2002), who indicated several major factors hampering progress in this area. The following factors found to be the most important bottle necks are discussed in the paper: (i) identification and utilization of new sources of resistance, not sufficient agronomic value of the used resistance sources; (ii) combination of earliness with LB resistance; (iii) complexity of genetic determination of LB resistance; (iv) combination of foliage and tuber resistance; (v) screening methods applied for resistance evaluation; (vi) cost of selection for resistance; (vii) lack of molecular markers (MAS) applicable to selection for LB resistance.   limitations, Phytophthora infestans, potato, resistance breeding
Źródło:
Plant Breeding and Seed Science; 2004, 50; 71-79
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Resistance to Phytophthora infestans in diploid and tetraploid potato families. 1. Resistance in detached leaflets
Autorzy:
Swiezynski, K M
Domanski, L
Osiecka, M
Sieczka, M T
Zarzycka, H
Powiązania:
https://bibliotekanauki.pl/articles/2046603.pdf
Data publikacji:
1997
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
tetraploid potato
Phytophthora infestans
inoculation
genotype
resistance
potato
detached leaflet
diploid potato
Opis:
Potato families, segregating for resistance to Phytophthora infestans, were tested repeatedly to evaluate the distribution of resistance to P. infestans, the repeatability of testing results and correlation between resistance and some other characters. Four diploid and four tetraploid families were evaluated together with their parents and with potato cultivars used as standard. For all inoculations a virulent fungus isolate MP 245 was used. Leaflets were collected from plants growing in the field (summer tests) or in a glasshouse (autumn and spring tests). Segregation of major genes determining resistance was detectable in most families. In families originating from mating a resistant parent with a susceptible partner some progeny genotypes with resistance level of the resistant parent could be identified. In families originating from two parents showing only some resistance, a transgression of resistance could be found in the progeny. The expression of resistance depended on testing conditions. Some genotypes were consistently superior or inferior in resistance under all testing conditions, but often repeated evaluations of genotypes did not provide consistent results and significant interactions genotypes x tests were detectable.
Źródło:
Journal of Applied Genetics; 1997, 38, 1; 19-32
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The distribution and changes of Phytophthora infestans population in Latvia
Autorzy:
Bebre, Gunta
Skrabule, Ilze
Osa, Marija
Powiązania:
https://bibliotekanauki.pl/articles/2198975.pdf
Data publikacji:
2004-12-20
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
control
late blight
potato
race
resistance
Opis:
Potato breeders and phytopathologists have been conducting the studies on Phytophthorainfestans (Mont.) de Bary at the Priekuli Plant Breeding Station since 1962. The varietal resistanceis very important in potato breeding programme. The trials in collaboration with the Institute ofPhytopathology of Soviet Union (Russia) were done in 1974-1990. The investigations were focusedon races of P. infestans. The changes of races were assessed using a trap method, according to theinvestigation programme, like in each of the republics of the Soviet Union.At the beginning of investigations (1960s and 1970s) races 1, 4 and 1.4 of P. infestans were thepredominant ones in Latvia, but in the 1980s complex races 4.10.11, 1.4.7.8.10.11, 1.3.4.7.8.10.11and 1.2.3.4.7.8.10.11 were more widely distributed.Different foreign varieties, mostly from the Netherlands, were introduced in Latvia in the 1970s.This resulted in a very rapid differentiation of late blight races. The enhanced aggressiveness of P.infestans isolates was due to an increasing number of races in population. The earlier appearance ofinfection in potato fields was recorded. The presence of a mating type A2 was stated in Latviansamples in 1987. Because all tested races had been recognized in a population, and the conception ofresistance breeding had been modified, the trials were stopped in 1990. The resistance based onspecific resistance genes was the main direction in the 1960s and 1970s. From the 1980s, impor-tance of the field resistance has greatly increased and this type of resistance is the most significantin the current breeding programme. The trials in organic field proved the acceptable level of fieldresistance to P. infestans of three medium late varieties: Sigunda (previous name Undine), Beteand Zile. These varieties have been bred at the Priekuli Plant Breeding Station and included intothe Latvian Plant Varieties Catalogue.
Źródło:
Plant Breeding and Seed Science; 2004, 50; 43-49
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Assessment of Potato Resistance to Synchytrium Endobioticum
Autorzy:
Przetakiewicz, Jarosław
Plich, Jarosław
Powiązania:
https://bibliotekanauki.pl/articles/2199702.pdf
Data publikacji:
2017-12-20
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
potato wart disease
resistance
molecular markers
Opis:
In Poland the Plant Breeding and Acclimatization Institute - National Research Institute (IHAR-PIB) is responsible for officially assessing the resistance of potato breeding lines and cultivars to Synchytrium endobi-oticum. In the official assessment of wart resistance the modified Glynne-Lemmerzahl method is used. A full cycle of assessment of the wart disease resistance requires 42 - 45 tubers per cultivar/breeding line. Forty two tubers are used in laboratory tests. To complete the laboratory tests, another 10 tubers are inoculated with winter sporangia of the fungus, using ring test. The final results are available after 3 years of investigation. If necessary, the full cycle of resistance tests to S. endobioticum can be performed during one year on 15 tubers in each of the 3 replications (45 tubers in total).Molecular verification of potato genotypes resistance to pathotype 1(D1) is possible with the use of SCAR marker Nl25-1400. Nevertheless, an official phenotypical assessment of advanced breeding lines, as the final verification of their resistance, is required.
Źródło:
Plant Breeding and Seed Science; 2017, 76; 37-43
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Reliability of different parameters to estimate relative foliage blight resistance and its relation to maturity in potato
Autorzy:
Darsow, Ulrich
Hansen, Jens G.
Powiązania:
https://bibliotekanauki.pl/articles/2198953.pdf
Data publikacji:
2004-12-20
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
breeding
foliage blight
methods
potato
Phytophthora infestans
resistance
Opis:
A comparative study of different parameters calculated for foliage resistance of potato to Phytophthora infestans (Mont.) de Bary was conducted on the data for 27 cultivars and one prebreeding clone in field assessment in 2003 at BAZ Groß Lüsewitz. Relationships between the parameters and maturity were determined by linear regression. The strongest associations with maturity were found for the relative area under the disease progress curve (RAUDPC), delay of attack, and attack on a determined date (r2 = 0.47-0.52). The least association with maturity was found for foliage blight resistance at Groß Lüsewitz, FBRGL (r2 = 0.03), calculated by using a maturity-dependent section of the disease progress curve. The apparent infection rate (AIR), RAUDPC and delay of attack could only explain below 50% of variability of FBRGL. The method for calculation of foliage resistance in breeding developed at BAZ and named FBRGL is described in detail, and the usefulness of the different methods for evaluation of resistance, management of plant protection and breeding is discussed.
Źródło:
Plant Breeding and Seed Science; 2004, 50; 81-93
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
In vitro evaluation of potato genotypes for resistance against bacterial soft rot (Pectobacterium carotovorum) - a new tool for studying disease resistance
Autorzy:
Azadmanesh, Sima
Mozafari, Javad
Hasanzadeh, Nader
Moslemkhani, Cobra
Powiązania:
https://bibliotekanauki.pl/articles/961645.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
in vitro
potato soft rot
resistance
screening method
Opis:
In vitro screening techniques were used to evaluate 46 genotypes of Iranian potato collection for resistance to bacterial soft rot caused by Pectobacterium carotovorum subsp. carotovorum (Pcc). One month old in vitro rooted potato plantlets were inoculated by two inoculation techniques under in vitro conditions: 1) sterile toothpicks dipped into bacterial suspension and pressed into the crown of plantlets and 2) the freshly cut crown of plantlets were dipped into bacterial suspension of 108 cfu ∙ ml–1 for 10 min. Typical soft rot disease symptoms, including the percentage of wilted leaves were recorded on inoculated plantlets 3, 6, 9, 12 and 15 days post-inoculation. The potato genotypes which were examined responded differently to Pcc and varying levels of resistance were observed. Potato genotype AG showed the highest level of resistance. Results obtained from in vitro screening were then verified by classical tuber slice assay. The verifications were conducted using five representative cultivars: Milva, Ramus, Picaso, Marfona and Agria which responded similarly to both in vitro and classical evaluation systems. Similar results obtained from these tests indicated that the in vitro screening technique developed in this study could provide a simple and rapid whole plant assay in selecting resistant potato genotypes against bacterial soft rot.
Źródło:
Journal of Plant Protection Research; 2017, 57, 1
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Resistance to Phytophthora infestans in diploid and tetraploid potato families. 3. Correlations between characters
Autorzy:
Swiezynski, K M
Domanski, L
Flis, B
Osiecka, M
Sieczka, M T
Powiązania:
https://bibliotekanauki.pl/articles/2047151.pdf
Data publikacji:
1997
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
tetraploid
Phytophthora infestans
resistance
tuber shape
potato
diploid
Opis:
In four diploid and four tetraploid families correlations were evaluated between resistance to Phytophthora infestans of leaflets, tuber slices and whole tubers as well as correlations between three aspects of resistance and maturity or tuber shape regularity. A virulent P. infestans isolate was used for inoculations. Only in the family D4 a significant positive correlation was found between all the three aspects of resistance. Genotypes with susceptible leaflets and resistant tuber slices were not identified in any family. In some families genotypes with resistant whole tubers and susceptible tuber slices were found; the family D5 was found to have genotypes in which resistance of leaflets was associated with susceptibility of tuber slices. The resistance to P. infestans of leaflets or tuber slices tended to be negatively correlated with both early maturity and regularity of tuber shape.
Źródło:
Journal of Applied Genetics; 1997, 38, 2; 161-171
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Assessing resistance to late blight of potato: methods used at the Scottish Crop Research Institute
Autorzy:
Stewart, Helen E.
Solomon-Blackburn, Ruth
Powiązania:
https://bibliotekanauki.pl/articles/2198944.pdf
Data publikacji:
2004-12-20
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
late blight
Phytophthora infestans
potato
resistance breeding
resistance test
screening methods
Solanum tuberosum
Opis:
At SCRI, wild species and clones of Solanum tuberosum are assessed for resistance to late blight in order to study the genetics and breed for resistance. Glasshouse progeny tests for foliage and tuber blight resistance, using true seedlings, are described. These enable the rapid screening of accessions of wild species, and the selection of the most resistant progenies in a breeding programme within one year of crossing. Assessment of the foliage resistance of clones is carried out in glasshouse tests of whole plants in flower-bud, and field trials using infector plants inoculated in the glasshouse with a complex race of Phytophthora infestans. The glasshouse test provides a reliable method of identifying R-genes and the virulence characteristics of blight isolates. The field trial gives the best estimate of field resistance and is being used to develop marker-assisted selection. Both tests are used to study the inheritance of resistance, to locate quantitative trait loci (QTL) and R-genes, and to select the most resistant clones. The tuber resistance of clones is assessed by spray-inoculating whole, immature, field-grown tubers on the day of harvest. However when large numbers of clones are involved, e.g. in the location of QTL, this is impractical, so glasshouse-grown tubers are dip-inoculated.
Źródło:
Plant Breeding and Seed Science; 2004, 50; 107-112
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Selection of frost-tolerant cell lines from cell cultures of Solanum tuberosum L.
Autorzy:
Anjum, Muhammad Akbar
Powiązania:
https://bibliotekanauki.pl/articles/2198823.pdf
Data publikacji:
2001-06-21
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
potato
Solanum
cell culture
proline
hydoxyproline resistance
frost tolerance
Opis:
Fourteen hydroxyproline-resistant cell lines were selected by plating 7 days old cell suspensions of Solanum tuberosum L. cvs. Desiree and Maris Piper on a cell plating medium containing 5 or 10 mM hydroxyproline (hyp). Cell suspensions were either plated directly on selective media or after mutagenic treatment with gamma rays at a dose of 20 Gy or after freezing to –6°C. The frequency of resistant colonies varied from 0.15 to 0.35 x 10-6. Almost all the selected lines possessed increased levels of frost tolerance as compared to their non-selected controls except one indicating that hyp resistance and frost tolerance are not necessarily linked.
Źródło:
Plant Breeding and Seed Science; 2001, 45, 1; 3-10
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Evaluation of Potato Cultivars and Breeding Lines for Resistance to Globodera Rostochiensis and Globodera Pallida
Autorzy:
Przetakiewicz, Anna
Milczarek, Dorota
Powiązania:
https://bibliotekanauki.pl/articles/2199697.pdf
Data publikacji:
2017-12-20
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
golden nematodes
potato cyst nematodes (PCN)
methods
resistance sources
Opis:
Nematodes are among the most important agents affecting potato crops. Heavy infestations by Globodera rosto-chiensis and G. pallida can cause significant yield losses and limit the choice of potato cultivars that can be grown effectively (Oerke, 2006). Breeding of new potato cultivar resistant to G. rostochiensis and G. pallida is a long-term process. It is preceded by screening of potato breeding lines for resistance in repeated biotests, which seems to be the most effective and safest method of protection. Evaluation of nematode resistance is an important element of charac-terization of breeding lines and new cultivars. Resistance to Globodera spp. is evaluated in biological tests. The use of DNA markers for detecting nematode resistance genes may be an alternative approach to phenotypic evaluation of resistance degree of potato plants (Jena and Mackill, 2008). The goal of this report is focused on a description of resistance assessment procedure of breeding lines and varieties of potato to PCN and on comparison of biological and molecular methods of resistance evaluation. Presented information is addressed to both breeders and Polish inspec-tion services.
Źródło:
Plant Breeding and Seed Science; 2017, 76; 3-8
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Resistance to PVM in potato parental lines bred in Młochów Research Center, IHAR.
Autorzy:
Chrzanowska, Mirosława
Sieczka, Maria Teresa
Zagórska, Helena
Powiązania:
https://bibliotekanauki.pl/articles/2199953.pdf
Data publikacji:
2002-12-22
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
potato
PVM
resistance genes Rm
Gm
reaction to infection
Opis:
During last 22 years in the Potato Genetics and Parental Lines Department at Młochów Research Center more than 210 parental lines resistant to Potato virus M (PVM) have been bred. Genes controlling resistance to PVM originated from two sources Solanum megistacrolobum (the gene Rm) or/and S. gourlayi (the gene Gm) were introduced into tetraploid breeding material. From these resistant lines 59 progenitors were chosen and offered to the potato breeders. The first resistant to PVM cultivar Triada and second one cv. Korona were registered in Poland in 1996 and 2002, respectively. At present 13 advanced breeding clones from different breeding stations derived from progenitors offered to breeders in 1986-1990 are evaluated in Preliminary Trials. One candidate cultivar tested in Registration Trials appeared to be resistant to PVM. Moreover 15 advanced breeding clones derived from progenitors offered during 1991-1995 were tested in Preliminary Trials. The mechanical and graft inoculations were used to identify the type of resistance to PVM in potatoes. The parental lines, which resistance to PVM is controlled by the gene Rm or/and Gm, express field resistance even under the high natural infection pressure. Increasing number of resistant to PVM new potato cultivars is expected.
Źródło:
Plant Breeding and Seed Science; 2002, 46, 2; 57-65
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Potencjał elektryczny bulwy ziemniaka
Potato tuber electric potential
Autorzy:
Marks, N.
Jakubowski, T.
Nawara, P.
Powiązania:
https://bibliotekanauki.pl/articles/288898.pdf
Data publikacji:
2010
Wydawca:
Polskie Towarzystwo Inżynierii Rolniczej
Tematy:
potencjał elektryczny
ziemniak
rezystancja
napięcie
electric potential
potato
resistance
voltage
Opis:
Potencjał elektryczny jest jednym z podstawowych parametrem opisującym pole elektryczne. Wyniki badań wskazują, że bulwa ziemniaka wytwarza napięcie w przedziale 0,7-8,2 mV a wartość napięcia elektrycznego wzrasta wraz ze wzrostem masy bulwy. Zależność między wartością napięcia i czasem pomiaru ma charakter nieliniowy. Podkreślić należy, że właściwości elektryczne dowolnej substancji uwarunkowane są przewodnością właściwą (konduktywnością), przenikalnością elektryczną, rezystancją i tangensem kąta stratności.
Electric potential is one of primary parameters describing electric field. Test results indicate that potato tuber generates voltage within range of 0.7-8.2 mV, and voltage value increases with growing tuber mass. The relation between voltage value and measurement duration has non-linear character. It should be emphasised that electrical properties of any substance are determined by specific conductance (electrical conductivity), permittivity, resistance and lossiness angle tangent.
Źródło:
Inżynieria Rolnicza; 2010, R. 14, nr 3, 3; 127-133
1429-7264
Pojawia się w:
Inżynieria Rolnicza
Dostawca treści:
Biblioteka Nauki
Artykuł
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