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    Wyświetlanie 1-2 z 2
    Tytuł:
    Kinetin affects the level of chloroplast polyamines and transglutaminase activity during senescence of barley leaves
    Autorzy:
    Sobieszczuk-Nowicka, Ewa
    Wieczorek, Przemysław
    Legocka, Jolanta
    Powiązania:
    https://bibliotekanauki.pl/articles/1040582.pdf
    Data publikacji:
    2009
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    barley leaves
    senescence
    transglutaminases
    chloroplasts
    kinetin
    polyamines
    Opis:
    We analysed the level of polyamines (PAs) bound to thylakoids and the level and activity of thylakoid transglutaminases throughout barley leaf senescence, retarded by kinetin. The level of PAs bound to thylakoids changed in senescing barley leaves: bound putrescine (PU) and spermidine (SD) increased throughout senescence, whereas bound spermine (SM) decreased. Kinetin diminished the increase in thylakoid-bound PU and SD and almost completely abolished the decrease of the bound SM. These data suggest different roles of PU/SD and SM in thylakoid degradation. Immunodetection of transglutaminases (TGase) in thylakoid fraction revealed three bands of 33, 58 and 78 kDa. During senescence the intensity of all bands increased and it was correlated with an increase in TGase activity. Kinetin down-regulated the accumulation of the 58- and 78-kDa TGases and the TGase activity. We postulate that formation of covalent bonds between PAs and proteins by TGase is involved in chloroplast senescence. The kinetin-mediated preservation of low TGase levels and activity throughout leaf senescence may represent an important component of the mechanism of kinetin action in the retardation of leaf senescence.
    Źródło:
    Acta Biochimica Polonica; 2009, 56, 2; 255-260
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Aminy biogenne w aspekcie ich roli w organizmach żywych
    Biogenic amines in their role in living systems
    Autorzy:
    Jastrząb, R.
    Tylkowski, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/171885.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Chemiczne
    Tematy:
    poliaminy
    aminy biogenne
    związki kompleksowe
    nowotwory
    polyamines
    biogenic amines
    coordination compounds
    tumors
    Opis:
    Although polyamines (PA) belong to relatively simple aliphatic substances, their role in life processes of animals and plants is of key importance [1–5]. The group of the most important amines, called biogenic ones includes: Spermine (Spm): H2N(CH2)3NH(CH2)4NH(CH2)3NH2 Spermidine (Spd): H2N(CH2)3NH(CH2)4NH2 Putrescine (Put): H2N(CH2)4NH2. Of secondary importance are homologues of biogenic amines, occurring in lower contents in living organisms [2, 6–8]: 1,3-diaminopropan: H2N(CH2)3NH2 Cadaverine: H2N(CH2)5NH2 Homospermidine: H2N(CH2)4NH(CH2)4NH2 Norspermine (3,3,3-tet): H2N(CH2)3NH(CH2)3NH(CH2)3NH2 Thermospermine: H2N(CH2)3NH(CH2)4NH(CH2)4NH2 Caldopentamine: H2N(CH2)3NH(CH2)3NH(CH2)3NH(CH2)3NH2. The first polyamine discovered in a living organism was tetramine, a spermine crystallised out of sperm in 1678 by Van Leewenkeuk [9]. Putrescine was discovered in the end of the 19th century in microbes and then triamine: spermidine was discovered in the beginning of the 20th century [2]. Later studies have shown that in animal cells spermidine and spermine occur at elevated levels, while in prokaryotes spermidine and putrescine contents are dominant. Putrescine, spermidine, 1,3-diaminopropan, homospermidine, norspermidine, and norspermine have been found in many gramnegative bacteria and algae [7, 10, 11]. Total concentration of PA in living organisms is on the order of millimols, however, the concentration of free polyamines is much lower. A low level of free amines follows from the fact that they are involved in noncovalent interactions with biomolecules occurring in living organisms such as nucleic acids, proteins, or phospholipids. High concentrations of non-bonded polyamines have been detected first of all in young molecules in the process of growth, in particular in rapidly proliferating cancer cells [6, 12]. Elevated levels of free polyamines have been observed, e.g. in breast, colon, lung, prostate, and skin tumours, accompanied by changed levels of enzymes responsible for biosynthesis and catabolism of polyamines. Because of the increased level of free polyamines and a tendency of their interaction with nucleic acids and other bioligands, these compounds have become objects of intense study [1, 13–19]. There is no doubt that the regulation of biosynthesis of polyamines and catabolism is one of the most important pathways in the search strategy for chemoprevention and chemotherapeutic drugs [14, 15, 20–36]. The present state of knowledge of these processes, their significance in biological systems, and their application in medicine are presented in subsequent sections of this chapter.
    Źródło:
    Wiadomości Chemiczne; 2016, 70, 1-2; 57-79
    0043-5104
    2300-0295
    Pojawia się w:
    Wiadomości Chemiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-2 z 2

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