Temat: ploidy determination - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Estimation of the share of components of different ploidy in anisoploid sugar-beet [Beta vulgaris L.] populations by flow cytometry
Autorzy:: Sliwinska, E
Jansen, R
Powiązania:: https://bibliotekanauki.pl/articles/2046829.pdf
Data publikacji:: 1997
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: Beta vulgaris
ploidy determination
breeding
flow cytometry
seed production
sugar-beet
Opis:: Flow cytometry is commonly used in sugar-beet breeding and seed production for ploidy estimation. By means of this method individual plants as well as plant populations can be analysed. An interpretation of histograms obtained from anisoploid populations, however, causes some problems to the breeder. Bulks of diploid, triploid and tetraploid sugar-beet plants in different proportion and ploidy combinations (2x + 3x, 2x + 4x, and 3x + 4x) were tested in order to determine the relation between the actual share of the components of different ploidy levels in tested populations and that calculated on the basis of flow cytometry. In this way agraph for estimation of the share of different ploidy components was obtained. Repeatability of the results proves the usefulness of flow cytometric analysis of anisoploid plant populations in breeding and seed production of polyploid crops.
Źródło:: Journal of Applied Genetics; 1997, 38, 2; 151-160
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Flow cytometric estimation of ploidy in anisoploid sugar-beet populations
Autorzy:: Sliwinska, E
Steen, P.
Powiązania:: https://bibliotekanauki.pl/articles/2048141.pdf
Data publikacji:: 1995
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: production
Beta vulgaris
poliploid cultivar
ploidy determination
nuclear DNA
flow cytometry
DNA
seed
sugar-beet
Opis:: Determination of ploidy level is a common practice in sugar-beet breeding and seed production. Lately many laboratories have replaced microscopic chromosome counting by flow cytometric estimation of the nuclear DNA content. The possibility of direct estimation of cytological composition in anisoploid populations instead of individual plant analyses was investigated. Diploid, triploid and tetraploid sugar-beet lines as well as their mixtures were tested. The results obtained show that the suggested method is fast, simple and, after a proper transformation of the computer values, could be applied in breeding and seed production of polyploid crops. Precise estimation of contamination by small admixture of seeds or plants of undesirable ploidy, however, requires individual plant rather than population analyses.
Źródło:: Journal of Applied Genetics; 1995, 36, 2; 111-118
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Cytologic ploidy determination in fish - an example of two salmonid species
Autorzy:: Jankun, M.
Kuzminski, H.
Furgala-Selezniow, G.
Powiązania:: https://bibliotekanauki.pl/articles/363096.pdf
Data publikacji:: 2007
Wydawca:: Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:: akwakultura
ryby łososiowate
poziom ploidii
manipulacje genomowe
aquaculture
salmonid species
ploidy level determination
genome manipulation
Opis:: The method of ploidy level determination depends on the equipment, expertise of the lab or farm staff, and on the material studied (fish species, specimens' age and size, etc.). The combination of some simple techniques can be very helpful for fast, reliable and inexpensive assessment of effects of genome manipulations. Among indirect methods the nucleoli quantification seems to be the most convenient one, except that it is limited to species possessing one locus of nucleolar organizer regions (NORs) (rainbow trout as an example). Species with multilocus NORs (like brook trout) need another method, for instance sizing of erythrocytes nuclei. These techniques can be useful for identification of products resulting from new approaches in biotechnology applied to fish culture, as polyploidisation or hybridisation, for characteristics of transformation levels of organisms found in the wild or those produced due to experimental treatments.
Źródło:: Environmental Biotechnology; 2007, 3, 2; 52-56
1734-4964
Pojawia się w:: Environmental Biotechnology
Dostawca treści:: Biblioteka Nauki

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