- Tytuł:
- Phenylethanoid glycosides in Plantago media L. organs obtained in in vitro cultures
- Autorzy:
-
Budzianowska, A.
Kikowska, M.
Malkiewicz, M.
Karolak, I.
Budzianowski, J. - Powiązania:
- https://bibliotekanauki.pl/articles/2117896.pdf
- Data publikacji:
- 2019
- Wydawca:
- Polska Akademia Nauk. Czasopisma i Monografie PAN
- Tematy:
-
acteoside
direct and indirect organogenesis
hoary plantain
micropropagation
phenyle-
thanoids
plantamajoside
TLC densitometry - Opis:
- Micropropagation of Plantago media L. and the presence of phenolic compounds in organs of multiplied plants were investigated for the first time. Multiplication of plant material was achieved in shoot-tip cultures and via direct organogenesis on Murashige and Skoog (MS) medium with four variants of plant growth regulators (M1–M4). The best multiplication coefficient – 9.2 was obtained in seedling shoot-tip cultures on MS medium M3 with BA 0.2 mg/L and IAA 1.0 mg/L. Methanol extracts prepared separately from shoots and roots of in vitro- derived plantlets were found to contain typical of the genus Plantago L. phenylethanoid glycosides as the only phenolics. Acteoside and plantamajoside were the major compounds – both known to possess a wide range of promising biological activities applicable for medicinal (therapeutic) and cosmetic uses. Martynoside, as a trace constituent, was also found for the first time in the studied species. The quantitative screening of the extracts by TLC video densitometric method showed a higher content of acteoside in shoots (range 62.43–93.03 mg/g, dry weight) and plantamajoside in roots (range 22.45–44.08 mg/g); the highest recorded values – 93.03 mg/g and 44.08 mg/g, respectively, were found in the organs obtained on MS medium M4 with BA 2.0 mg/L.
- Źródło:
-
Acta Biologica Cracoviensia. Series Botanica; 2019, 61, 1; 75-86
0001-5296 - Pojawia się w:
- Acta Biologica Cracoviensia. Series Botanica
- Dostawca treści:
- Biblioteka Nauki
Artykuł