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    Tytuł:
    Influence of light conditions on the morphogenetic and biochemical response of selected ornamental plant species under in vitro conditions: a mini-review
    Autorzy:
    Kulus, D.
    Woźny, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/2096967.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    horticulture
    LED panels
    plant tissue culture
    regeneration
    secondary metabolites
    somatic
    embryogenesis
    Opis:
    In vitro tissue culture technique, especially micropropagation, is attracting increasing attention in the production of ornamental plants. This technique will probably dominate the horticultural market in the near future. Light is one of the several factors affecting the success of in vitro plant tissue cultures. It directly affects the widely understood morphogenetic response of the explant, i.e., the ability of the explant to grow or regenerate, produce roots, etc. Lighting conditions provided during the in vitro stage may also greatly affect the plant vigor after transferring to nonsterile conditions. Moreover, the necessity of providing artificial light significantly contributes to the total cost of maintaining tissue cultures (related to energy consumption and the need to cool down the heat generated by lamps). Light quantity (intensity) and quality (spectral composition) are the two main parameters that determine its influence on in vitro cultures. This impact depends on the species and other accompanying factors. The aim of this mini-review is to summarize information on the influence of light on the morphogenetic and biochemical response of explants of some selected ornamental plant species grown under in vitro conditions.
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2020, 101, 1; 75-83
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vitro culture as a powerful method for conserving Iranian ornamental geophytes
    Autorzy:
    Hesami, M.
    Naderi, R.
    Yoosefzadeh-Najafabadi, M.
    Maleki, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/80634.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    in vitro culture
    plant tissue culture
    ornamental plant
    geophyte
    cryopreservation
    genetic resource
    biodiversity
    germplasm
    Iran
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2018, 99, 1
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Protocol for acclimatization of in vitro cultured Potamogeton praelongus - aspect of plantlet size and type of substrate
    Autorzy:
    Prausova, R.
    Kozelkova, Z.
    Safarova, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/57604.pdf
    Data publikacji:
    2015
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    acclimatization
    in vitro culture
    Potamogeton praelongus
    plant tissue culture
    growth experiment
    plantlet size
    substrate type
    Opis:
    The aim of the experiment was to determine suitable substrate type and optimal plant size for transfer of plantlets from in vitro to ex vitro under experimental outdoor conditions. Tests focused on the effect of substrate type (muddy and sandy) and starting size of plantlets gained through in vitro seed germination (0-3, 3.1-5,5.1-6, 6.1-10 cm) on plant growth. Three parameters (fresh weight, length, and the number of leaves) were compared to evaluate growth. Basic water parameters in experimental water tanks were regularly measured (pH, temperature, electrical conductivity, shadow intensity) and controlled to reach similar conditions to those in the natural habitat of this species. Overwintering was studied in a cellar with newly defined size categories (<6, 6.1-8, 8.1-10, 10.1-12, 12.1-15 cm). Both substrate type and starting size of plantlets significantly impacted growth. Plantlets grew better in the muddy substrate while a 100% success rate of rooting was gained with a starting size of 6.1-10 cm in both substrates. The biggest increase in fresh weight was observed with a starting size of 3.1-5 cm and 5.1-6 cm in both substrates. The greatest increase in fresh weight was observed in plants with a starting size of 3.1-5 cm in the muddy substrate (more than 95% increase). The best overwintering results were gained in the 6.1-8 cm size category.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2015, 84, 1
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Adjustments to in vitro culture conditions and associated anomalies in plants
    Autorzy:
    Isah, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/19921.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    plant physiology
    plant tissue culture
    plant regeneration
    in vitro culture
    somaclonal variation
    hyperhydricity
    fasciation
    cresting zob.fasciation
    acclimatization
    plant
    anomaly
    Źródło:
    Acta Biologica Cracoviensia. Series Botanica; 2015, 57, 2
    0001-5296
    Pojawia się w:
    Acta Biologica Cracoviensia. Series Botanica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vitro flowering and micropropagation of Lisianthus (Eustoma grandiflorum) in response to plant growth regulators (NAA and BA)
    Kwitnienie i mikropropagacja in vitro lisianthusa (Eustoma grandiflorum) w reakcji na regulatory wzrostu roslin (NAA i BA)
    Autorzy:
    Kaviani, B.
    Zamiraee, F.
    Zanjani, S.B.
    Tarang, A.
    Torkashvand, A.M.
    Powiązania:
    https://bibliotekanauki.pl/articles/11542950.pdf
    Data publikacji:
    2014
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    in vitro culture
    ornamental plant
    flowering
    micropropagation
    Lisianthus
    Eustoma grandiflorum
    plant response
    plant growth regulator
    NAA potassium salt
    axillary bud
    callus
    plant tissue culture
    Opis:
    In vitro flowering and micropropagation are useful for plant breeding programs and commercial production of important ornamental plants. In vitro conditions including media components, kind, concentration and ratio of plant growth regulators and culture conditions significantly affect in vitro flowering and micropropagation. There is no any report dealing with the in vitro flowering of Lisianthus (Eustoma grandiflorum). Here, a protocol was developed for flowering and high frequency in vitro micropropagation of E. grandiflorum, an ornamental plant. Micropropagation is an effective tools for propagation of ornamental plants in large scale. The aim of the present study was to evaluate the effect of different concentrations of NAA and BA on micropropagation and flowering of Lisianthus, in vitro. Used culture medium was MS enriched with 0, 0.1, 0.2 and 2 mg L-1 of NAA and BA. In establishment process of explants, the most shoot length (2.07 cm per plant) was obtained on medium supplemented with 0.1 mg L-1 BA (without NAA). Maximum shoot number (5.80 per plant) was produced in medium containing 0.1 mg L-1 BA along with 0.2 mg L-1 NAA. Bud explants in culture media containing 0.2 mg L-1 NAA (without BA) and 0.1 mg L-1 NAA along with 2 mg L-1 BA produced maximum node number (3.20 per plant). The largest number of root (14.53 per plant) and root length (3.87 cm per plant) were produced on 0.2 mg L-1 NAA without BA, also 0.2 mg L-1 BA plus 0.2 mg L-1 NAA and 0.2 mg L-1 BA without NAA. Explants produced flower on medium containing 0.1 mg L-1 BA along with 0.1 mg L-1 NAA without transition of callus formation. Flower was produced from callus in medium containing 0.1 mg L-1 BA along with 2 mg L-1 NAA. Regenerated plants showed 98% survival in greenhouse during acclimatization. Acclimatized plants were morphologically similar to the mother plants.
    Kwitnienie i mikropropagacja in vitro są użyteczne w programach hodowli roślin oraz produkcji komercyjnej ważnych roślin ozdobnych. Warunki in vitro, łącznie ze składnikami pożywek, rodzajem, stężeniem oraz proporcją regulatorów wzrostu roślin, a także warunkami hodowli, w sposób istotny wpływają na kwitnienie i mikropropagację in vitro. Nie istnieje żadne badanie dotyczące kwitnienia in vitro lisanthiusa (Eustoma grandiflorum). W niniejszym badaniu opracowano kwitnienie i wysoką częstotliwość mikropropagacji in vitro dla E. grandiflorum, który jest rośliną ozdobną. Mikropropagacja jest skutecznym narzędziem rozmnażania roślin ozdobnych na dużą skalę. Celem niniejszego badania była ocena wpływu różnych stężeĔ NAA i BA na mikropropagację i kwitnienie lisianthiusa in vitro. Używana pożywka hodowlana została wzbogacona za pomocą 0; 0,1; 0,2 i 2 mg L-1 NAA i BA. Przy powstawaniu eksplantów największa długość łodygi (2,07 cm na roślinę) była uzyskana na pożywce uzupełnionej o 0,1 mg L-1 BA (bez NAA). Maksymalna liczba łodyg (5,80 na roślinę) została wytworzona na pożywce zawierającej 0,1 mg L-1 BA wraz z 0,2 mg L-1 NAA. Eksplanty pączków na pożywce hodowlanej zawierającej 0,2 mg L-1 NAA (bez BA) oraz 0,1 mg L-1 NAA wraz z 2 mg L-1 BA wytworzyły maksymalną liczbę węzłów (3,20 na roślinę). Największą liczbę korzeni (14,53 na roślinę) oraz największą długość korzenia (3,87 na roślinę) zaobserwowano na 0,2 mg L-1 NAA bez BA jak również 0,2 mg L-1 BA plus 0,2 mg L-1 NAA oraz 0,2 mg L-1 BA bez NAA. Eksplanty tworzyły kwiat na pożywce zawierającej 0,1 mg L-1 BA wraz z 0,1 mg L-1 NAA bez przeniesienia kalusa. Kwiat był tworzony z kalusa na pożywce zawierającej 0,1 mg L-1 BA wraz z 2 mg L-1 NAA. Zregenerowane rośliny wykazały 98% przeżycie w szklarni podczas aklimatyzacji. Zaaklimatyzowane rośliny były morfologicznie podobne to swych roślin macierzystych.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2014, 13, 4; 145-155
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Methods of eliminating contaminations in tissue cultures on the example of selected ornamental plant species
    Autorzy:
    Parzymies, M.
    Dabski, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/951204.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conference
    tissue culture
    contamination
    ornamental plant
    Clematis
    biological contamination
    micropropagation
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vitro responses of various explants of Fagus sylvatica
    Autorzy:
    Hazubska-Przybyl, T.
    Chmielarz, P.
    Bojarczuk, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/41768.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    European beech
    plant response
    explant
    Fagus sylvatica
    micropropagation
    culture condition
    tissue culture
    in vitro culture
    Opis:
    There are limited published data on in vitro reproduction of Fagus sylvatica L. (European beech). This study was aimed to determine the efficiency of induction of somatic embryogenesis or organogenesis of beech from different types of explants in various culture conditions. Explants derived from immature, fresh seeds (collected in 2011 and 2013) and from mature seeds, stored at –10ºC and some stratified at 3ºC, were placed on induction media with various combinations of plant growth regulators: zeatin, 2,4-dichlorophenoxyacetic acid (2,4-D) and/or benzyladenine (BA). Initial cultures were kept in darkness or weak light (white fluorescent or blue-red LED). Limited success has been achieved in initiation of somatic embryogenesis. We obtained friable, yellow-white callus with characteristic PEM-like structures (cPEM-ls, from embryonic axes or fragments of immature embryos with embryonic axes), which may be an early developmental stage of embryogenic callus of Fagus sylvatica. This type of callus regenerated from explants incubated in darkness, mainly on WPM medium with addition of 6.8 μM zeatin or WPM and MSG media with 9.1 μM 2,4-D and 2.2 μM BA. The highest frequency of regeneration of callus with cPEM-ls was 5%. Instead, we succeeded to induce organogenesis from both immature and mature zygotic embryos and from embryonic axes. The best results were obtained for mature zygotic embryos incubated on ½WPM medium (half-strength Woody Plant Medium) with 9.1 μM 2,4-D and 2.2 μM BA. Adventitious buds were regenerated on up to 15% of the explants. The induced buds developed into shoots, enabling us to establish tissue cultures of beech. Induction of organogenesis from the tested explants was more efficient than induction of somatic embryogenesis.
    Źródło:
    Dendrobiology; 2015, 73
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    65 years of in vitro culture in Poland
    Autorzy:
    Zenkteler, M.
    Zenkteler, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/58090.pdf
    Data publikacji:
    2013
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    in vitro culture
    history
    plant tissue
    in vitro technology
    Polska
    Opis:
    This paper is a short review of historical development of the tissue culture method in Poland. Similarly to the rest of the world, in vitro technology in Poland has progressed in many directions simultaneously. Its main fields are closely interconnected by natural sequences of biological processes and integrade one into another. The best results, driven by the prospects of practical applications, have been achieved within the areas of meristem culture and micropropagation of valuable genotypes, somaclonal variation, production of haploids and DH plants, somatic embryogenesis, in vitro culture of embryos and ovules, improvement of plant disease resistance, somatic hybridization and transformation of plants. Due to the fact that in vitro domain is a very broad science the authors are aware that this review might not fully cover all the scientists working with tissue culture and their achievements.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2013, 82, 3
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Rozmnazanie ziemniaka 'in vitro' w Instytucie Ziemniaka w Boninie
    Autorzy:
    Zaklukiewicz, K
    Sekrecka, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/835057.pdf
    Data publikacji:
    1992
    Wydawca:
    Instytut Hodowli i Aklimatyzacji Roślin
    Tematy:
    ziemniaki
    kultury tkankowe
    rozmnazanie roslin
    hodowla in vitro
    potato
    tissue culture
    plant propagation
    in vitro culture
    Źródło:
    Ziemniak Polski; 1992, 3; 41-44
    1425-4263
    Pojawia się w:
    Ziemniak Polski
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The influence of stress conditions on acclimation of magnolia Magnolia x soulangiana Soul.-Bod.
    Wpływ warunków stresu na aklimatyzację magnolii Magnolia x soulangiana Soul.-Bod.
    Autorzy:
    Kamenicka, A.
    Lanakova, M.
    Cerna, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/808264.pdf
    Data publikacji:
    2002
    Wydawca:
    Szkoła Główna Gospodarstwa Wiejskiego w Warszawie. Wydawnictwo Szkoły Głównej Gospodarstwa Wiejskiego w Warszawie
    Tematy:
    stress condition
    acclimation
    magnolia
    Magnolia x soulangiana
    tissue culture
    leaf cell
    donor plant
    Opis:
    Under a scanning microscope, leaves from tissue cultures (after 8 weeks in vitro culturing), from 1 year old regenerants and from the 100 years old donor tree were studied. More abnormalities were observed in the tissue culture compared to regenerant and donor. During tissue culture transfer rapid, leaf dehydration occurs due to defective regulation mechanisms making stomata of tissue culture stay open. Epidermis as well as stomata cells were observed already on the leaves in in vitro conditions, but they become physiologically functioning only after transfer into conditions of natural environment.
    Przy zastosowaniu mikroskopu scaningowego porównywano cechy budowy komórek liści pochodzących z 100 letniej rośliny matecznej, 8 tygodniowych kultur tkankowych oraz 1 rocznych regeneratów. W regenerantach z kultur tkankowych obserwowano więcej nienormalności w budowie komórek epidermy, komórek szparkowych i komórek przyszparkowych w porównaniu z komórkami pochodzącymi z rośliny matecznej. Podczas przenoszenia tkanek w kulturze in vitro obserwowano odwodnienie tkanek liści a mimo to komórki szparkowe pozostawały otwarte. Komórki epidermy oraz komórki szparkowe były obecne na liściach hodowli in vitro jednak stawały się one fizjologicznie funkcjonalne tylko wtedy gdy zostały przeniesione do warunków naturalnych.
    Źródło:
    Zeszyty Problemowe Postępów Nauk Rolniczych; 2002, 481, 1
    0084-5477
    Pojawia się w:
    Zeszyty Problemowe Postępów Nauk Rolniczych
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The influence of explants type and orientation on growth and development of Mandevilla sanderi (Hemsl.) Woodson in vitro
    Autorzy:
    Kozak, D.
    Parzymies, M.
    Świstowska, A.
    Marcinek, B.
    Ismael, B.S.
    Powiązania:
    https://bibliotekanauki.pl/articles/12663898.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    plant cultivation
    Brazilian jasmine
    Mandevilla sanderi
    ornamental plant
    flowering plant
    pot plant
    commercial plant
    plant growth
    plant development
    plant regeneration
    explant type
    multiplication
    decapitation
    defoliation
    in vitro culture
    tissue culture
    cultivation experiment
    Opis:
    Mandevilla sanderi is an important commercial ornamental pot plant. Traditional vegetative propagation is limited due to the low rate, therefore there is a need to develop an alternative, more efficient method. There is an interest in development of micropropagation technology for the species, as it allows to obtain a lot of offsprings in a relatively short time. The aim of the present work was to estimate an influence of explants type and position on regeneration of Mandevilla sanderi in tissue culture. Four different types of explants (leafy shoot tips, decapitated leafy shoot tips, defoliated shoot tips, decapitated and defoliated shoot tips) were used in the experiment, which were placed on the media vertically, while defoliated shoot tips were placed horizontally or vertically upside down. The explants were cultivated on a Murashige and Skoog (MS) medium supplemented with 1 mg·dm–3 benzyladenine (BA) and 0.5 mg·dm–3 indole-3-butyric acid (IBA). It was noted that both explants orientation and positioning, influenced the multiplication rate. Defoliated shoot tips placed horizontally were characterized by higher multiplication rate (6.8) in comparison to upside down vertical positioning (3.2). It was also observed that removal of shoot apex improved axillary branching, while defoliation of shoots placed in a normal position reduced multiplication rate.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 4; 111-119
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Bank genow 'in vitro' - stan obecny
    Autorzy:
    Zaklukiewicz, K
    Sekrecka, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/836124.pdf
    Data publikacji:
    1993
    Wydawca:
    Instytut Hodowli i Aklimatyzacji Roślin
    Tematy:
    hodowla roslin
    ziemniaki
    kultury tkankowe
    hodowla in vitro
    banki genow
    odmiany roslin
    plant breeding
    potato
    tissue culture
    in vitro culture
    gene bank
    plant cultivar
    Źródło:
    Ziemniak Polski; 1993, 2; 28-36
    1425-4263
    Pojawia się w:
    Ziemniak Polski
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Comparative study on tissue culture induced variation identified with metAFLP and RP – HPLC in barley and triticale regenerants
    Autorzy:
    Machczynska, J.
    Orlowska, R.
    Ogorek, K.A.
    Bednarek, P.T.
    Powiązania:
    https://bibliotekanauki.pl/articles/951298.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    tissue culture
    in vitro culture
    plant regeneration
    genetic change
    DNA methylation
    histone modification
    high performance liquid chromatography
    barley
    triticale
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The influence of PPM upon the sterility of the in vitro cultures in European beech [Fagus sylvatica L.]
    Autorzy:
    Kraj, W
    Dolnicki, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/56550.pdf
    Data publikacji:
    2003
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    Plant Preservative Mixture
    plant physiology
    tissue culture
    tree bud
    Fagus sylvatica
    sterilization
    beech
    sterility
    in vitro
    embryo
    European beech
    bud
    Opis:
    Investigations were conducted on the strongly infested by microbes embryos isolated from seed stratified in non-sterile conditions or from tree buds. The results showed, that PPM (Plant Preservative Mixture, produced by Plant Cell Technology, Inc.) was very useful for increasing the percentage of sterile cultures disinfected in a 5% solution of NaOCl. The best way of applying PPM turned out to be adding the substance directly to the growing medium. At the concentration of 2 or 4 cm3 x dm-3 of PPM there was an 30% increase in percentage of sterile of in vitro cultures developed from embryos and 70-80% of cultures developed from buds, as compared with the control without PPM. At the range of concentrations employed in this study, no negative effects of PPM upon the development and growth of callus were observed.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2003, 72, 4
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Neural modeling of plant tissue cultures: a review
    Autorzy:
    Zielinska, S.
    Kepczynska, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/81293.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    artificial neural network
    biomass
    plant tissue
    neural model
    tissue culture
    in vitro condition
    micropropagation
    radial neural network
    neural network
    somatic embryo
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The use of nano-silver for disinfection of Pennisetum alopecuroides plant material for tissue culture
    Autorzy:
    Parzymies, M.
    Pudelska, K.
    Poniewozik, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/12683367.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    Pennisetum alopecuroides
    grass species
    ornamental grass
    shoot
    explant
    plant material
    tissue culture
    plant growth regulator
    micropropagation
    contamination
    plant regeneration
    silver
    nanoparticle
    disinfection
    Opis:
    Initiation of tissue culture of many plant species is a very difficult stage due to appearance of many contaminations. The other problem might be a choice of media for regeneration. Initiation of grass species tissue cultures are thought to be very difficult. Therefore, a research was undertaken to evaluate the use of nano-silver particles for plant material disinfection and to estimate a medium Pennisetum alopecuroides. The plant material were buds and nodal explants that were disinfected in 2% NaOCl for 30 min or 0.1% HgCl2 for 1 min. Half of the explants disinfected with NaOCl were soaked in 50, 100 or 250 mg·dm–3 Ag NPs for 1 hour. Explants not soaked in nano-silver were placed on media with Ag NPs at concentrations of 4, 8 or 16 mg·dm–3. An influence of growth regulators on Pennisetum alopecuroides was evaluated in vitro. Regenerated shoots were placed on MS media with: 3 mg·dm–3 BA + 0.3 mg·dm–3 IBA, 3 mg·dm–3 KIN + + 0.3 mg·dm–3 IAA, 1 mg·dm–3 BA + 0.1 mg·dm–3 IBA. It was observed that the use of nano-silver particles lowered the level of contamination. The best results were obtained when Ag NPs was used at concentration of 100–250 mg·dm–3 alone or as a supplementation of the media, at concentration of 4 mg·dm–3 for nodes and 16 mg·dm–3 for adventitious buds. The use of nodal explants allowed to obtain less contamination. Regeneration depended on a media content. The most regenerated shoots were obtained on the MS media supplemented with 1 mg·dm–3 BA and 0.1 mg·dm–3 IBA.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 3; 127-135
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A non caloric sweetener Stevia rebaudiana Bertoni - tissue cultured plantlets for organic farming and home gardening
    Autorzy:
    Tomaszewska-Sowa, M.
    Figas, A.
    Sawilska, A.K.
    Powiązania:
    https://bibliotekanauki.pl/articles/59692.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Stowarzyszenie Infrastruktura i Ekologia Terenów Wiejskich PAN
    Tematy:
    non-caloric sweetener
    sweetener
    Stevia rebaudiana
    micropropagation
    plant growth regulator
    tissue culture
    plantlet
    organic farming
    home garden
    Źródło:
    Infrastruktura i Ekologia Terenów Wiejskich; 2015, IV/3
    1732-5587
    Pojawia się w:
    Infrastruktura i Ekologia Terenów Wiejskich
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Somatic embryogenesis and plant regeneration from hypocotyl and leaf explants of Brassica oleracea var. botrytis (cauliflower)
    Autorzy:
    Siong, P.K.
    Taha, R.M.
    Rahiman, F.A.
    Powiązania:
    https://bibliotekanauki.pl/articles/19314.pdf
    Data publikacji:
    2011
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    somatic embryogenesis
    plant regeneration
    hypocotyl
    leaf explant
    Brassica oleracea var.botrytis
    cauliflower
    embryogenic callus
    callus
    tissue culture
    Opis:
    We investigated direct and indirect formation of somatic embryogenesis in Brassica oleracea var. botrytis (cauliflower), a very important vegetable crop worldwide. Direct somatic embryogenesis, which is rather rare, was achieved in culture of 2-week-old hypocotyl explants of Brassica oleracea var. botrytis on MS medium supplemented with 1.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5; 1.0; and 1.5 mg/l kinetin. Initial induction of embryogenic callus was achieved on MS supplemented with very low concentrations of 2,4-D (0.05 mg/l and 0.1 mg/l). Indirect somatic embryogenesis from leaf sections was obtained on MS supplemented with 0.05 or 0.1 mg/l 2,4-D. We examined various stages of somatic embryos (globular, heart, torpedo, cotyledonary). More embryos per explant were produced through the indirect pathway (23–25) than through the direct pathway (14–19). The number of embryos produced was high. There is a potential for recurrent, repeated or secondary somatic embryogenesis, possibly an unlimited source for mass propagation and ideal for synthetic seed production in this species. Plant regeneration was achieved on half-strength MS medium without any hormones.
    Źródło:
    Acta Biologica Cracoviensia. Series Botanica; 2011, 53, 1
    0001-5296
    Pojawia się w:
    Acta Biologica Cracoviensia. Series Botanica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Withania coagulans (Stocks) Dunal: biotechnological achievements and perspectives
    Autorzy:
    Teixera da Silva, J.A.
    Kher, M.M.
    Soner, D.
    Nataraj, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/1928.pdf
    Data publikacji:
    2015
    Wydawca:
    Instytut Ogrodnictwa
    Tematy:
    Withania coagulans
    medicinal plant
    Solanaceae
    shoot regeneration
    rooting
    in vitro
    micropropagation
    tissue culture
    random amplified polymorphic DNA
    Źródło:
    Journal of Horticultural Research; 2015, 23, 1
    2300-5009
    Pojawia się w:
    Journal of Horticultural Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The influence of growth regulators on dahlia propagation in tissue culture and acclimatization of plants in ex vitro conditions
    Autorzy:
    Marcinek, B.
    Parzymies, M.
    Poniewozik, M.
    Kozak, D.
    Durlak, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/12307379.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    plant cultivation
    Dahlia
    Dahlia x cultorum
    dahlia
    ornamental plant
    flowering plant
    tuberous plant
    tuberous root
    plant acclimatization
    growth regulator
    cytokinin
    gibberellic acid
    micropropagation
    tissue culture
    ex vitro method
    microcutting
    Opis:
    The aim of the work was to evaluate the influence of cytokinins on in vitro propagated dahlia and their consequent effect on acclimatization. Plant material consisted of shoot tips and nodes. Among the three cytokinins, benzyladenine, kinetin and 2-isopentenyl-adenine, only BA effectively stimulated the shoot multiplication from axiliary buds. The highest multiplication rate was obtained from nodes in the presence of 0.25– 0.5 mg·dm–3 BA. Higher concentrations shortened the internodes and decreased the leaf blades and growth of callus. 1 mg·dm–3 of KIN and 2iP positively influenced the shoot growth and size of leaves. Gibberellic acid (GA3) used with BA increased the number of auxillary shoots. The best quality shoots and the highest multiplication rate were obtained when 2 mg·dm–3 BA was used with 5 mg·dm–3 GA3. Cytokinins affected the rooting and acclimatization ex vitro. Dahlia shoots multiplicated in the presence of 1 mg·dm–3 KIN or 2iP rooted faster in the soil and 100% survived in field, while those from 1 mg·dm–3 BA media rooted slowly, had shorter shoots and only 60% of them survived. Plants bloomed after 11–12 weeks in the field. Dahlia plants that had been multiplicated in the presence of KIN had larger diameter and fresh weight in the field. BA and 2iP positively influenced the flower diameter, length of flower stalk and a number of the first-order shoots.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 5; 49-61
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    An improved procedure for in vivo and in vitro propagation of Buxus hyrcana, an ornamental shrub critically at risk of extinction
    Autorzy:
    Negahdar, N.
    Hashemabadi, D.
    Kaviani, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/80583.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    ornamental shrub
    Buxus hyrcana
    endangered plant
    plant growth regulator
    rooting
    stem cutting
    tissue culture
    indole-3-butyric acid
    kinetin
    in vitro propagation
    in vivo
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2019, 100, 4
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Comparison of Allium aflatunense B. Fedtsch. ‘Purple Sensation’ and Allium karataviense Regel. ‘Ivory Queen’ regenerative capabilities in tissue culture
    Porównanie zdolnosci regeneracyjnych Allium aflatunense B. Fedtsch. 'Purple Sensation' i Allium karataviense Regel. 'Ivory Queen' w kulturze in vitro
    Autorzy:
    Kozak, D.
    Stelmaszczuk, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/11542509.pdf
    Data publikacji:
    2013
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    comparison
    Allium aflatunense
    ornamental plant
    Allium karataviense
    regenerative capability
    tissue culture
    inflorescence stem
    leaf base
    polarity explant
    shoot multiplication
    Opis:
    Allium aflatunense and Allium karataviense are valuable bulbous plants grown in a garden. Moreover, Allium aflatunense is one of the most decorative species for cut flowers industry. These plants characterize with a low multiplication rate. Immature inflorescence stem sections and leaf bases isolated from ‘Allium aflatunense ‘Purple Sensation’ and Allium karataviense ‘Ivory Queen’ bulbs in October were used as source of explants for shoot regeneration. The use of a two – step sterilization procedure (2% Cl- – 30 min and 1% Cl- –15 min) gave much better results than a one – step (2% Cl- – 30 min). Inflorescence explants in inverted orientation were cultured on a Murashige and Skoog [1962] medium containing BA in concentration of 2 mg·dm⁻³ and NAA in concentration of 1 mg·dm⁻³. Leaf bases placed in normal and inverted orientation were incubated on MS medium supplemented with: BA 2 mg·dm⁻³ plus NAA 1 mg·dm⁻³, BA 5 mg·dm⁻³ plus NAA 1 mg·dm⁻³ and TDZ 1 mg·dm⁻³ plus NAA 1 mg·dm⁻³. In subsequent experiments clumps consisting of 2 shoots were cultured on MS medium containing BA 0.5, 1, 2 mg·dm⁻³ with NAA 0.1 mg·dm⁻³ and BA 2 mg·dm⁻³ with IAA 0.1 mg·dm⁻³ for 6 weeks. A multiplication rate during four subcultures on medium with addition of BA 0.5 mg·dm⁻³ and NAA 0.1 mg·dm⁻³ was also examined. Explants from young inflorescences and from immature leaf bases were found effective to form shoots. The orientation of leaf explants did not have a significant effect on the number of regenerating shoots. 5.7 to 7.5 shoots were achieved for Allium aflatunense ‘Purple Sensation’ on studied media during 6 weeks of multiplication and 3.5 to 5.1 shoots for Allium karataviense ‘Ivory Queen’. Multiplication rate of 31.3 shoots for Allium aflatunense ‘Purple Sensation’ and 26.6 shoots for Allium karataviense ‘Ivory Queen’ was possible to obtain on medium supplemented with BA 0.5 mg·dm⁻³ and NAA 0.1 mg·dm⁻³ after 32 weeks of culture.
    Allium aflatunense i Allium karataviense to cenne rośliny cebulowe do uprawy w ogrodach. Ponadto Allium aflatunense jest jednym z najbardziej dekoracyjnych gatunków uprawianym na kwiat cięty. Rośliny te charakteryzują się niskim współczynnikiem rozmnażania. Źródłem materiału do zapoczątkowania kultur były fragmenty młodych pędów kwiatostanowych i nasady liści izolowane w paĨdzierniku z Allium aflatunense ‘Purple Sensation’ i Allium karataviense ‘Ivory Queen’. Zastosowanie sterylizacji dwukrotnej (2% Cl- – 30 min i 1% Cl- –15 min) dało lepsze rezultaty niż sterylizacja pojedyncza. Eksplantaty z pędów kwiatostanowych wykładano w pozycji odwrotnej do naturalnej i kultywowano na pożywce Murashige i Skooga [1962] zawierającej BA 2 mg·dm⁻³ i NAA 1 mg·dm⁻³. Podstawy liści układano w pozycji naturalnej i odwróconej i inkubowano na pożywce MS z dodatkiem: BA 2 mg·dm⁻³ + NAA 1 mg·dm⁻³, BA 5 mg·dm⁻³ + NAA 1 mg·dm⁻³ i TDZ 1 mg·dm⁻³ + NAA 1 mg·dm⁻³. W kolejnym doświadczeniu zespoły pędów zawierające 2 pędy namnażano na pożywkach MS uzupełnionych: BA 0,5; 1; 2 mg·dm⁻³ oraz NAA 0,1 mg·dm⁻³ i BA 2 mg·dm⁻³ oraz IAA 0,1 mg·dm⁻³ podczas 6 tygodni. Oceniono również współczynnik namnażania pędów podczas 4 pasaży na pożywce z dodatkiem BA 0,5 mg·dm⁻³ i NAA 0,1 mg·dm⁻³. Fragmenty młodych pędów kwiatostanowych i podstawa liści wykazywały duże zdolności regeneracyjne pędów. Sposób ułożenia eksplantatów nie wywierał istotnego wpływu na liczbę regenerujących pędów. Na badanych pożywkach uzyskano od 5,7 do 7,5 pędów Allium aflatunense ‘Purple Sensation’ i od 3,5 do 5,1 pędów Allium karataviense ‘Ivory Queen’ podczas 6 tygodni namnażania. Podczas 32 tygodni namnażania pędów na pożywce zawierającej BA 0,5 mg·dm⁻³ i NAA 0,1 mg·dm⁻³ uzyskano 31,3 pędów Allium aflatunense ‘Purple Sensation’ i 26,6 pędów of Allium karataviense ‘Ivory Queen’.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2013, 12, 6; 197-213
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Somaclonal variation in winter wheat [Triticum aestivum L.]: frequency, occurrence and inheritance
    Autorzy:
    Cheng, X Y
    Gao, M.W.
    Liang, Z.Q.
    Liu, G.Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/2044461.pdf
    Data publikacji:
    1998
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    inheritance
    tissue culture
    Triticum aestivum
    occurrence
    in vitro
    winter wheat
    gene mutation
    plant breeding
    callus induction
    frequency
    somaclonal variation
    wheat
    embryo
    Opis:
    Plants were regenerated from immature embryo cultures of 35 winter wheat genotypes. General responses of regenerated plants were investigated and a total of 7142 R₂ spike lines from 1593 R₁ plants were assessed in the field for somaclonal variants in 1985/86, 1986/87 and 1987/88. Selected variants were studied for their possible genetic inheritance. From regenerated plantlets, 81% survived and 63% produced fertile plants. Forms with reduced plant height, length of spike and other morphological abnormalities were found in this progeny. Populations of R₁ plants were highly variable due mainly to the physiological disturbances resulting from the in vitro process. Overall somaclonal variation frequencies were 14.2% per plant basis and 5.3% per R₂ spike basis. The variants were similar in the three different R₂ generations with predominant variants being negative in plant height, maturity, awns, spike type and plant type. Both uniform R₂ variant families and spike lines were found in addition to the segregating variants which constituted the majority. On average, in a variant family or line, 18% and 14% of their component lines and plants were variants, respectively. Inheritability was demonstrated for the uniform variant families and spike lines as well as segregated variants. Of those 134 selections, about 70% were classified as inheritable. Both recessive and dominant gene mutations at one, two or three loci were evident in some variants as suggested by the segregating data.
    Źródło:
    Journal of Applied Genetics; 1998, 39, 1; 59-72
    1234-1983
    Pojawia się w:
    Journal of Applied Genetics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Apoplastic markers of cell differentiation status during somatic embryogenesis in Arabidopsis thaliana
    Autorzy:
    Potocka, I.
    Sala, K.
    Kurczynska, E.U.
    Powiązania:
    https://bibliotekanauki.pl/articles/81256.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conference
    somatic embryogenesis
    Arabidopsis thaliana
    plant tissue
    embryogenic culture
    immature zygotic embryo
    cotyledonary stage
    meristematic cell
    arabinogalactan protein
    lipid transfer protein
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Applications of recent advances at the Institute of Grassland and Environmental Research in cytogenetics of the Lolium-Festuca complex
    Autorzy:
    Humphreys, M W
    Thomas, H M
    King, I P
    Morgan, W G
    Meredith, M R
    Harper, J A
    Humphreys, M O
    Bettany, A J E
    Dalton, S J
    James, A R
    Ougham, H J
    Thomas, H
    Powiązania:
    https://bibliotekanauki.pl/articles/2046675.pdf
    Data publikacji:
    1997
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    RNA
    in situ
    tissue culture
    gene transfer
    chromosome segment
    anther culture
    androgenesis
    plant breeding
    introgression mapping
    fluorescence
    hybridization
    Lolium-Festuca complex
    drought resistance
    gene isolation
    tetraploid hybrid
    DNA
    meiosis
    grass
    chromosome behaviour
    Opis:
    Recent advances at Institute of Grassland and Environmental Research (Aberystwyth, U.K.) in cytogenetics of the Lolium/Festuca complex places us in the advantageous position of being able to map genes of agronomic importance onto chromosome arms using fluorescence in situ hybridization (FISH). The ability to physically map genes leads to the capability for "dissecting" quantitative traits into their different components and will lead to better understanding of the complex physiological processes involved and the identification of their genetic control. By tagging genes of interest, using molecular and morphological markers, it will be possible to select and combine suites of desirable genes in a single genotype and thus produce novel cultivars by conventional breeding procedures. Programmes for introgression depend on the relationships between species and on levels of chromosome pairing. Phylogenetic relationships within the Lolium/Festuca complex are being determined using both genomic in situ hybridization (GISH) and FISH. With recent advances in genetic manipulation within the Lolium/Festuca complex, opportunities now arise for gene transfer from Lolium and Festuca species into other important agricultural crops.
    Źródło:
    Journal of Applied Genetics; 1997, 38, 3; 273-284
    1234-1983
    Pojawia się w:
    Journal of Applied Genetics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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