Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

Wyszukujesz frazę "plant identification" wg kryterium: Temat


Tytuł:
Hybrid texture and gradient modeling for dynamic background subtraction identification systemin tobacco plant using 5G data service
Autorzy:
Gowda Thirthe, M.T.
Chandrika, J.
Powiązania:
https://bibliotekanauki.pl/articles/38699145.pdf
Data publikacji:
2023
Wydawca:
Instytut Podstawowych Problemów Techniki PAN
Tematy:
background subtraction
local binary pattern
tobacco plant
texture
Gaussian mixture model
illumination change
plant disease identification system
usuwanie tła
lokalny wzorzec binarny
tytoń
tekstura
model mieszaniny Gaussa
zmiana oświetlenia
system identyfikacji chorób roślin
Opis:
Background: Detecting the plants as objects of interest in any vision-based input sequence is highly complex due to nonlinear background objects such as rocks, shadows,etc. Therefore, it is a difficult task and an emerging one with the development of precision agriculture systems. The nonlinear variations of pixel intensity with illuminationand other causes such as blurs and poor video quality also make the object detection taskchallenging. To detect the object of interest, background subtraction (BS) is widely usedin many plant disease identification systems, and its detection rate largely depends on thenumber of features used to suppress and isolate the foreground region and its sensitivitytoward image nonlinearity. Methodology: A hybrid invariant texture and color gradient-based approach is proposed to model the background for dynamic BS, and its performance is validated byvarious real-time video captures covering different kinds of complex backgrounds and various illumination changes. Based on the experimental results, a simple multimodal featureattribute, which includes several invariant texture measures and color attributes, yieldsfinite precision accuracy compared with other state-of-art detection methods. Experimental evaluation of two datasets shows that the new model achieves superior performanceover existing results in spectral-domain disease identification model. 5G assistance: After successful identification of tobacco plant and its analysis, the finalresults are stored in a cloud-assisted server as a database that allows all kinds of 5G servicessuch as IoT and edge computing terminals for data access with valid authentication fordetailed analysis and references.
Źródło:
Computer Assisted Methods in Engineering and Science; 2023, 30, 1; 41-54
2299-3649
Pojawia się w:
Computer Assisted Methods in Engineering and Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ekwiwalencja a zjawisko „false friends w słowiańskich nazwach roślin
Equivalence and false friends in Slavic plant names
Autorzy:
Waniakowa, Jadwiga
Powiązania:
https://bibliotekanauki.pl/articles/2158331.pdf
Data publikacji:
2021-12-29
Wydawca:
Polska Akademia Nauk. Instytut Języka Polskiego PAN
Tematy:
false friends
equivalence
plant name identification
polysemy
homonymy
ekwiwalencja
identyfikacja roślin
polisemia
homonimia
Opis:
Artykuł dotyczy podobieństw międzyjęzykowych i zjawiska false friends w słowiańskich nazwach roślin. We wszystkich słowiańskich dialektach i w historii języków słowiańskich można zaobserwować, że jedna nazwa może odnosić się do kilku gatunków roślin, a dany gatunek może mieć nawet kilkadziesiąt nazw. Jak pokazują analizy, nierzadko nazwy te są zgodne formalnie i semantycznie na dużych obszarach, na których mówi się językami słowiańskimi. W podsumowaniu artykułu zasugerowano, że badania porównawcze materiałów słowiańskich zawsze wymagają dużej ostrożności, aby zapobiec ewentualnym błędom w identyfikacji. Podobieństwo formalne nie gwarantuje adekwatności treści, czyli prawdziwego znaczenia, gdyż w niektórych przypadkach mamy do czynienia z całkowitą równoważnością nazw i ich desygnatów w danych językach słowiańskich, a czasem nazwy takie stanowią false friends.
The article deals with interlingual similarities and the phenomenon of false friends in Slavic plant names. In all Slavic dialects and in the history of Slavic languages it is observed that one name may refer to several species of plants, and a given species may have even dozens of names. As analyses show, it is not uncommon for these names to be formally and semantically compatible across large areas where Slavic languages are spoken. In the conclusion of the paper, it is suggested that comparative studies of Slavic materials always require great caution to prevent possible mistakes in identification. Formal similarity does not guarantee the adequacy of the content, i.e. the true meaning, as in some cases we deal with complete equivalence of the names and their designates in the given Slavic languages, and sometimes the names constitute false friends.
Źródło:
Polonica; 2021, 41; 39-49
0137-9712
2545-045X
Pojawia się w:
Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A brief history of rose cultivation in Lithuania in the 18th–19th centuries. The legacy of old garden roses and their identification
Krótka historia uprawy róż na Litwie w XVIII i XIX w. Spuścizna dawnych róż ogrodowych i ich identyfikacja
Autorzy:
Ryliene, D.
Powiązania:
https://bibliotekanauki.pl/articles/888485.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Dendrologiczne
Tematy:
history
plant cultivation
rose
Lithuania
18th century
19th century
old garden rose
identification
Źródło:
Rocznik Polskiego Towarzystwa Dendrologicznego; 2018, 66
2080-4164
2300-8326
Pojawia się w:
Rocznik Polskiego Towarzystwa Dendrologicznego
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Enhancement of fungal DNA templates and PCR amplification yield by three types of nanoparticles
Autorzy:
Al-Dhabaan, F.A.
Yousef, H.
Shoala, T.
Shaheen, J.
El Sawi, Y.
Farag, T.
Powiązania:
https://bibliotekanauki.pl/articles/65369.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
plant pathology
detection
identification
plant pathogen
toxigenic fungi
improvement
specificity
efficiency
polymerase chain reaction
Alternaria alternata
DNA extraction
nanoparticle
Rhizoctonia solani
nanobiotechnology
Opis:
Nanodiagonastic methods in plant pathology are used for enhancing detection and identification of different plant pathogens and toxigenic fungi. Improvement of the specificity and efficiency of the polymerase chain reaction (PCR) by using some nanoparticles is emerging as a new area of research. In the current research, silver, zinc, and gold nanoparticles were used to increase the yield of DNA for two plant pathogenic fungi including soil-borne fungus Rhizoctonia solani and toxigenic fungus Alternaria alternata. Gold nanoparticles combined with zinc and silver nanoparticles enhanced both DNA yield and PCR products compared to DNA extraction methods with ALB buffer, sodium dodecyl sulfate, ALBfree from protinase K, ZnNPs and AgNPs. Also, by using ZnNPs and AgNPs the DNA yield was enhanced and the sensitivity of random amplified polymorphic DNA (RAPD) PCR products was increased. Application of nanomaterials in the PCR reaction could increase or decrease the PCR product according to the type of applied nanometal and the type of DNA template. Additions of AuNPs to PCR mix increased both sensitivity and specificity for PCR products of the tested fungi. Thus, the use of these highly stable, commercially available and inexpensive inorganic nano reagents open new opportunities for improving the specificity and sensitivity of PCR amplicon, which is the most important standard method in molecular plant pathology and mycotoxicology.
Źródło:
Journal of Plant Protection Research; 2018, 58, 1
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification and characterization of Pseudomonas syringae pv. syringae strains from various plants and geographical regions
Autorzy:
Khezri, M.
Mohammadi, M.
Powiązania:
https://bibliotekanauki.pl/articles/65669.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
identification
Pseudomonas syringae pv.syringae
plant
phenotype
polymerase chain reaction
protein profile
plasmid
syringomycin
geographic region
Opis:
Pseudomonas syringae pv. syringae (Pss) constitutes a diverse group of bacterial strains that cause canker of stone fruits, blight of cereals and red streak of sugarcane. The purpose of this study was to determine how diverse Iranian strains of Pss are when they come from different hosts. We compared a total of 32 Pss strains isolated from stone fruits, barley, wheat and sugarcane from different geographical regions of Iran based on their phenotypic and molecular properties. Strains showed some variation regarding carbon and nitrogen utilization. Pss strains were similar in their protein banding patterns. Additional bands were found in sugarcane strains. Most strains showed one indigenous plasmid DNA and a few had two and some none. The genes of syrB and syrD encoding syringomycin synthesis and secretion, respectively, were amplified using specific primers in polymerase chain reaction. Syringomycin, producing strains amplified two DNA fragments of 752 and 446 bp representing syrB and syrD genes, respectively. Primer specificity was shown for Pss using various genera. Based on the results of this study, it is suggested that Pss strains from different hosts and geographical regions show diversity in phenotypic and molecular characters. It is thought that phenotypic variation is due to adaptation to specific hosts and niches for survival and pathogenicity.
Źródło:
Journal of Plant Protection Research; 2018, 58, 4
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Isolation and identification of plant growth promoting rhizobacteria from maize (Zea mays L.) rhizosphere and their plant growth promoting effect on rice (Oryza sativa L.)
Autorzy:
Karnwal, A.
Powiązania:
https://bibliotekanauki.pl/articles/65251.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
isolation
identification
plant growth
genotyping
phytohormone
indoleacetic acid
promoting rhizobacteria
rhizobacteria
maize
Zea mays
rhizosphere
rice
Oryza sativa
Opis:
The use of plant growth promoting rhizobacteria is increasing in agriculture and gives an appealing manner to replace chemical fertilizers, pesticides, and dietary supplements. Th e objective of our research was to access the plant growth promotion traits of Pseudomonas aeruginosa, P. fl uorescens and Bacillus subtilis isolated from the maize (Zea mays L.) rhizosphere. In vitro studies showed that isolates have the potential to produce indole acetic acid (IAA), hydrogen cyanide, phosphate solubilisation, and siderophore. RNA analysis revealed that two isolates were 97% identical to P. aeruginosa strain DSM 50071 and P. aeruginosa strain NBRC 12689 (AK20 and AK31), while two others were 98% identical to P. fl uorescens strain ATCC 13525, P. fl uorescens strain IAM 12022 (AK18 and AK45) and one other was 99% identical to B. subtilis strain NCDO 1769 (AK38). Our gnotobiotic study showed signifi cant diff erences in plant growth variables under control and inoculated conditions. In the present research, it was observed that the isolated strains had good plant growth promoting eff ects on rice.
Źródło:
Journal of Plant Protection Research; 2017, 57, 2
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular identification of Candidatus Phytoplasma spp. associated with Sophora yellow stunt in Iran
Autorzy:
Allahverdi, T.
Rahimian, H.
Rastgou, M.
Powiązania:
https://bibliotekanauki.pl/articles/66588.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
molecular identification
phytoplasma symptom
sophora plant
Sophora alapecuroides
yellow stunt virus
leaf
yellowing
Iran
Opis:
In the spring of 2012, sophora (Sophora alopecuroides L.) plants showing symptoms of leaf yellowing, little leaves and stunting were observed in Firooz-kuh (Tehran province), Sari (Mazandaran province) and Urmia (West Azerbaijan province) in Iran. Symptomatic plants from the three locations were subjected to nested polymerase chain reaction (PCR) to amplify 16SrRNA using primer pair P1/P7 followed by primer pair R16F2n/R16R2. Th e amplicons were purifi ed, sequenced and the nucleotide sequences were analyzed by virtual restriction fragment length polymorphism (RFLP). Th e phytoplasmas associated with the yellows disease were identifi ed as members of the 16SrIX group (Candidatus Phytoplasma phoenicium) and the 16SrXII group (Candidatus Phytoplasma solani). Th e two phytoplasmas were placed in 16SrIX-C and 16SrXII-A subgroups, respectively, in constructed phylogenetic trees. Th is is the fi rst report on sophora yellows associated with Candidatus Phytoplasma phoenicium.
Źródło:
Journal of Plant Protection Research; 2017, 57, 2
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Skład biocenozy bakteryjnej osadu czynnego na przykładzie miejskiej oczyszczalni ścieków w Zgierzu
Composition of activated sludge biocenosis in the example of municipal wastewater treatment plant for a city of Zgierz
Autorzy:
Liwarska-Bizukojć, E.
Powiązania:
https://bibliotekanauki.pl/articles/237251.pdf
Data publikacji:
2017
Wydawca:
Polskie Zrzeszenie Inżynierów i Techników Sanitarnych
Tematy:
bacteria
identification
molecular biology
isolation of genomic DNA
DNA sequencing
wastewater treatment plant
activated sludge
oxidoreductive conditions
biocenosis
bakterie
identyfikacja
biologia molekularna
izolacja genomowego DNA
sekwencjonowanie DNA
oczyszczalnia ścieków
osad czynny
warunki tlenowe
biocenoza
Opis:
Molecular biology techniques allow for increasingly more thorough identification and understanding of functionality of microorganisms consortia involved in wastewater treatment or waste degradation processes. In this work, the bacterial community of activated sludge from the wastewater treatment plant in Zgierz (Poland) was identified. As a result, a somewhat limited database of microorganisms living in Polish wastewater treatment plants was supplemented with new data. The composition of bacterial community was identified with molecular biology techniques in two main stages: (1) isolation of genomic DNA, (2) DNA sequencing and bioinformatics analysis of the isolated sequences. The analyses revealed that the most abundant phylum was Proteobacteria with Betaproteobacteria present in highest numbers. Composition of the activated sludge was relatively low in Chloroflexi class bacteria. It is in alignment with macroscopic observations indicating good settlement properties of the activated sludge from the wastewater treatment plant in Zgierz. It was determined that aerobic (redox) conditions in the activated sludge chamber did not influence the composition of bacterial community. They might only cause increase in the contribution of aerobic bacteria in the aerated (oxygenated) part of the chamber or the anaerobic organisms in its anaerobic (deoxygenated) section.
Techniki biologii molekularnej pozwalają w coraz większym stopniu poznać skład i zrozumieć funkcjonowanie konsorcjów mikroorganizmów biorących udział w procesach oczyszczania ścieków czy biodegradacji odpadów. W pracy zidentyfikowano skład biocenozy osadu czynnego w oczyszczalni ścieków komunalnych w Zgierzu, dzięki czemu została poszerzona, jak na razie dość skromna, baza danych o mikroorganizmach w polskich oczyszczalniach ścieków. Skład biocenozy bakteryjnej zidentyfikowano za pomocą technik biologii molekularnej. Analiza składała się z dwóch zasadniczych etapów – izolacji genomowego DNA oraz sekwencjonowania wraz z analizą bioinformatyczną sekwencji. Badania wykazały, że na poziomie typu największy udział w biocenozie osadu czynnego miały Proteobacteria, a wśród nich najwięcej było bakterii należących do klasy Betaproteobacteria. Osad z oczyszczalni ścieków w Zgierzu wyróżniał się stosunkowo małą liczebnością bakterii z klasy Chloroflexi, co pokrywa się z obserwacjami makroskopowymi, wskazującymi na dobre właściwości sedymentacyjne tego osadu. Stwierdzono, że warunki tlenowe panujące w komorze osadu czynnego nie miały znaczącego wpływu na skład bakteryjny osadu czynnego. Mogły one powodować jedynie większy udział bakterii aerofilnych w części napowietrzanej komory (tlenowej) i/lub większy udział beztlenowców w jej części nienapowietrzanej (beztlenowej).
Źródło:
Ochrona Środowiska; 2017, 39, 4; 3-7
1230-6169
Pojawia się w:
Ochrona Środowiska
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Incidence of seed-borne fungi on Lupinus mutabilis depending on a plant morphotype, sowing date and plant density
Autorzy:
Pszczolkowska, A.
Okorski, A.
Kotecki, A.
Gas, M.
Kulik, T.
Reczek, A.
Powiązania:
https://bibliotekanauki.pl/articles/961386.pdf
Data publikacji:
2016
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie / Polskie Towarzystwo Magnezologiczne im. Prof. Juliana Aleksandrowicza
Tematy:
seed-borne fungi
Lupinus mutabilis
plant
seed health
seed yield
Andean lupin
macronutrient
morphotype
sowing date
plant density
Colletotrichum
identification
Opis:
Seeds of the Andean lupine are characterised by high nutritional value, and the plant could become an important crop in the production of food and forage. This legume continues to attract growing interest around the world. A field experiment was carried out in in Lower Silesia, Poland, in 2011-2012. Two Andean lupine morphotypes (indeterminate and determinate) were analysed. Andean lupine was grown in treatments characterised by different sowing dates and plant density per m2. Seed yield, macronutrient content, protein content and health were evaluated at harvest. Seed yield was determined by the interaction of all experimental factors. The indeterminate form produced a significantly higher yield than the determinate form, regardless of the sowing date. The factors had little influence on the mineral content of seeds and total protein content. Andean lupine seeds were colonised mostly by saprotrophic fungi of the genera Alternaria, Cladosporium, Epicoccum and Rhizopus and pathogenic fungi of the genera Botrytis, Colletotrichum and Fusarium. Delayed sowing contributed to seed colonisation by fungi of the genus Colletotrichum. The determinate form was more susceptible to infection than the indeterminate form. Molecular analysis showed that the Colletotrichum isolates found in the study belong to the Colletotrichum acutatum species complex. The pathogen causing lupine anthracnose, isolated from the seeds of Andean lupine in the present study, was identified as Colletotrichum lupini (within C. acutatum complex) in a molecular analysis, and its DNA sequence was compared with those of the isolates deposited in the GenBank.
Źródło:
Journal of Elementology; 2016, 21, 2
1644-2296
Pojawia się w:
Journal of Elementology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
9alpha-hydroxyparthenolide in Zoegea leptaurea subsp. mesopotamica (Czerep.) Rech. (Asteraceae)
9alfa-hydroksypartenolid w Zoegea leptaurea subsp. mesopotamica (Czerep.) Rech. (Asteraceae)
Autorzy:
Nawrot, J.
Dawid-Pac, R.
Kaczerowska-Pietrzak, K.
Urbanska, M.
Nowak, G.
Powiązania:
https://bibliotekanauki.pl/articles/72392.pdf
Data publikacji:
2015
Wydawca:
Instytut Włókien Naturalnych i Roślin Zielarskich
Tematy:
9-alpha-hydroxyparthenolide
isolation
identification
spectral analysis
plant material
Zoegea leptaurea ssp.mesopotamica
Compositae
chemotaxonomy
Opis:
From the aerial parts of Zoegea leptaurea subsp. mesopotamica (Czerep.) Rech. (syn Zoegea mesopotamica Czerep.), 9α-hydroxyparthenolide was isolated. This compound was identified by spectral methods (1H NMR and 13C NMR). This research confirmed earlier indications about the presence of 4,5-epoxygermacranolides in the Zoegea L. genus. Thus, distinctive chemistry feature of plants in this taxon has chemotaxonomic implications.
Z ziela Zoegea leptaurea subsp. mesopotamica (Czerep.) Rech. (syn Zoegea mesopotamica Czerep.) wyizolowano 9α-hydroksypartenolid. Związek ten zidentyfikowano za pomocą analiz spektralnych (1H NMR i 13C NMR). Potwierdzono tym samym wcześniejsze doniesienia o występowaniu 4,5-epoksygermakranolidów w rodzaju Zoegea L. Uzyskane dane mają walor chemotaksonomiczny.
Źródło:
Herba Polonica; 2015, 61, 2
0018-0599
Pojawia się w:
Herba Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A comparative and experimental study on gradient and genetic optimization algorithms for parameter identification of linear MIMO models of a drilling vessel
Autorzy:
Bańka, S.
Brasel, M.
Dworak, P.
Jaroszewski, K.
Powiązania:
https://bibliotekanauki.pl/articles/329756.pdf
Data publikacji:
2015
Wydawca:
Uniwersytet Zielonogórski. Oficyna Wydawnicza
Tematy:
MIMO dynamic plant
identification
nonlinear system
system MIMO
identyfikacja parametryczna
układ nieliniowy
Opis:
The paper presents algorithms for parameter identification of linear vessel models being in force for the current operating point of a ship. Advantages and disadvantages of gradient and genetic algorithms in identifying the model parameters are discussed. The study is supported by presentation of identification results for a nonlinear model of a drilling vessel.
Źródło:
International Journal of Applied Mathematics and Computer Science; 2015, 25, 4; 877-893
1641-876X
2083-8492
Pojawia się w:
International Journal of Applied Mathematics and Computer Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Proteomics of E.coli Nissle 1917 in response to Cocos nucifera sap and wine
Autorzy:
Chandrasekhar, K
Pramoda Kumari, J.
Powiązania:
https://bibliotekanauki.pl/articles/11862.pdf
Data publikacji:
2015
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
proteomics
Escherichia coli
plant response
coconut palm
Cocos nucifera
sap
wine
protein
probiotic
identification
scanning
Opis:
In the present study, we described the protein profile experimentally by 2D-PAGE and MALDI analysis to understand the stress mechanisms of cocoti sap and wine on E.coli Nissle 1917. We isolated one newly expressed protein from cocoti wine treated gel which is not present in both control and cocoti sap treated sample i.e. P21 prophage-derived head-stabilizing proteinVG03_ECOL6 (3n1) also called as Head protein gp3. This protein mainly activities related to the viral life cycle. It helps to attach the viral gene into host. The growth rate was delayed in cocoti wine treated E.coli Nissle 1917 when compared to control and cocoti sap treated samples. Stress mechanism induce many proteins they are involved in metabolic process, hydrolase activity, lyase activity, quinone binding, phosphotransferase system, carbohydrate metabolism, DNA binding, DNA repair, transferase activity, oxidoreductase, purine metabolism, transcription antitermination, transcription regulation and other related activities. We proved that the predicted protein structure quality, resolution, density and error plot values by QMEAN analysis. Based on these results, only two differentially expressed proteins under sap stress showed that the significant results, which were N-acetylgalactosamine-specific phosphotransferase enzyme IIB component 1, PTPB1_ECOLI and DinI-like protein Z3305/ECs2939 in prophage CP-933VDINI1_ECO57. In case of wine stress, the differentially expressed proteins were Transcription anti-termination protein RFAH- ECO57 NusA and PUR7- eco24- phosphoribosylamidazole-succinocarboxamide synthase showed significant results. ProtParam analysis indicating that the multiple physico-chemical characters of differentially expressed proteins were differed and compared. The phylogenetic tree represents the relationship in-between the differentially expressed proteins, were showed siblings (related) as well as monophytic clade.
Źródło:
International Letters of Natural Sciences; 2015, 41
2300-9675
Pojawia się w:
International Letters of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
First report of black-foot disease, caused by Cylindrocarpon destructans, on ornamental marigold (Tagetes minuta) in Iran
Autorzy:
Jamali, S.
Nasimi, Z.
Powiązania:
https://bibliotekanauki.pl/articles/65408.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
blackfoot disease
Cylindrocarpon destructans
ornamental plant
marigold
Tagetes minuta
herb
molecular identification
Iran
Opis:
The ornamental Tagetes minuta is a herbaceous plant of the Asteraceae family. T. minuta, a species native to southern South America, is used as a condiment, as a refreshing beverage, and for medicinal purposes. In 2011, disease symptoms of yellowing, root and foot rot, drying of leaves, and plant death were observed in an ornamental marigold (T. minuta) greenhouse in Fars province. The infected plants were collected and transferred to a laboratory. Samples were washed, cut into small pieces, surface disinfested with a 0.5% NaClO solution, and cultured on Potato Dextrose Agar (PDA) acidified to pH 4.5 with 0.5% lactic acid. Based on morphological characters, the causal agent was identified as Cylindrocarpon destructans. To confirm morphological identification, DNA was extracted from isolates using a genomic DNA purification Kit. The region of internal transcribed spacers 1, 2, and 5.8S genes of rDNA were amplified using the ITS4 and ITS1 universal primer set. Fragments of 600 bp were recovered from PCR, purified, sequenced, edited, and deposited in GenBank. The isolates had a 100% identity with all the compared C. destructans sequences. The pathogenicity tests were done with a suspension of 1 × 106 conidia per ml homogenised in sterile water. The symptoms on inoculated plants were similar to those previously observed and the fungus was reisolated from the inoculated plants. This is the first documented report of C. de-structans as a cause of root and foot rot disease on T. minuta in Iran.
Źródło:
Journal of Plant Protection Research; 2014, 54, 2
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification and binomial computerization of plant species
Autorzy:
Karthik, K.
Pugalenthi, M.
Sharavanan, P.S.
Powiązania:
https://bibliotekanauki.pl/articles/11186.pdf
Data publikacji:
2014
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
identification
binomial computerization
plant species
new technology
computerization process
Opis:
The binomial computerization method is comparison of certain similarities and differences of plants identification system. The ultimate purpose of the plants identification process is method of using digital tools of database and creating new version of plants identification for constructing the digital keys. The present study of plant identification system is based on computerization processes. The plants species characters are constructing basis on taxonomical literature and followed by suitable classification system and binomial rules.
Źródło:
International Letters of Natural Sciences; 2014, 14
2300-9675
Pojawia się w:
International Letters of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of genetic diversity among Arnica montana L. genotypes using RAPD markers
Analiza zróżnicowania genetycznego wśród genotypów Arnica montana L. za pomocą markerów RAPD
Autorzy:
Okoń, S.
Paczos-Grzęda, E.
Łoboda, M.
Sugier, D.
Powiązania:
https://bibliotekanauki.pl/articles/11542962.pdf
Data publikacji:
2014
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:
DNA polymorphism
identification
genetic diversity
Arnica montana
genotype
RAPD marker
medicinal plant
molecular analysis
Opis:
Arnica montana L. is one of the most important herbal plants used in medicine, pharmaceutical and cosmetic industry. The number of studies performed with molecular markers on arnica genotypes is very limited. Because of this fact the aims of presented examination were optimization of protocols DNA isolation from fresh leaves of A. montana and identification of genetic diversity among this plant genotypes. In presented study to obtain pure DNA Plant & Fungi DNA Purification Kit (EURx) were used. To clean obtained DNA long and slow electrophoresis and isolation DNA from gels were used. A. montana genotypes were analyzed using 40 RAPD primers (Operon Technologies), out of which 12 produced high number of polymorphic and repeatable fragments. In total, selected primers produced 120 fragments, among them 111 (92.5%) were polymorphic. The genetic similarity matrices were produced based on RAPD using the Dice’s coefficient. RAPD based genetic similarity was estimated between 0.535 and 0.945. The highest genetic similarity was estimated among GA17 and GA18 genotypes, which are closely located on the obtained dendrogramme.
Arnica montana L. jest jedną z najcenniejszych roślin zielarskich wykorzystywanych w medycynie, farmacji i przemyśle kosmetycznym. W dostępnej literaturze liczba doniesień związanych z analizą molekularną arniki jest znikoma, dlatego też celem prezentowanych badań była optymalizacja procesu izolacji DNA ze świeżych liści oraz identyfikacja zróżnicowania genetycznego oparta na markerach RAPD. W prezentowanej pracy w celu uzyskania czystego DNA do izolacji wykorzystano zestaw DNA Plant & Fungi DNA Purification Kit (Euro) oraz oczyszczanie za pomocą długiej elektroforezy w żelu agarozowym. Spośród testowanych 40 starterów RPAD do analiz wybrano 12 generujących stabilne i polimorficzne wzory prążków. Wyselekcjonowane startery amplifikowały 120 fragmentów, spośród których 111 (92,5%) było polimorficznych. Wykorzystujac markery RAPD utworzono matryce podobieństwa genetycznego. średnia wartość podobieństwa analizowanych genotypów wynosiła 0.886. Najwyższy współczynnik podobieństwa genetycznego oszacowano pomiędzy genotypami GA17 i GA18, które ulokowały się blisko siebie na uzyskanym dendrogramie.
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2014, 13, 4; 63-71
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł

Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies