Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

English (EN)·Polski (PL)·Yкраїнський (UK)
Przejdź do strony domowej biblioteki Centralna Biblioteka Wojskowa Link otwiera się w nowym oknie Logo biblioteki Prolib Integro - strona głównaCentralna Biblioteka Wojskowa

Wyszukujesz frazę "phylogenetic analysis" wg kryterium: Temat

  Lista filtrów
Wyrównaj
Wyświetlanie 1-15 z 40
Tytuł:
Antimicrobial activities and phylogenetic study of Erythrina senegalensis DC (Fabaceae) seed lectin
Autorzy:
Enoma, Samuel
Adewole, Taiwo S.
Agunbiade, Titilayo O.
Kuku, Adenike
Powiązania:
https://bibliotekanauki.pl/articles/16672367.pdf
Data publikacji:
2023
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
antimicrobial
Erythrina senegalensis
lectin
phylogenetic analysis
polymerase chain reaction
Opis:
Erythrina senegalensis (Fabaceae) have been traditionally used in the treatment of microbial ailments, and the specific agent mediating its efficacy has been investigated in several studies. In this study, the antimicrobial activity of purified E. senegalensis lectin (ESL) was analyzed. The phylogenetic relationship of the gene encoding lectin with other legume lectins was also established to investigate their evolutionary relationship via comparative genomics. Antimicrobial activity of ESL against selected pathogenic bacteria and fungi isolates was evaluated by the agar well diffusion method, using fluconazole (1 mg/ml) and streptomycin (1 mg/ml) as positive controls for fungi and bacteria sensitivity, respectively. Potent antimicrobial activity of ESL against Erwinia carotovora, Pseudomonas aeruginosa, Klebsiella pneumonia, Staphylococcus aureus, Aspergillus niger, Penicillium camemberti, and Scopulariopsis brevicaulis was observed, with inhibition zones ranging from 18 to 24 mm. Minimum inhibitory concentrations of ESL ranged between 50 and 400 μg/ml. Primer-directed polymerase chain reaction of E. senegalensis genomic DNA detected a 465-bp lectin gene with an open reading frame encoding a 134-amino acid polypeptide. The obtained nucleotide sequence of the ESL gene shared high sequence homology: 100, 100, and 98.18% with Erythrina crista-galli, Erythrina corallodendron, and Erythrina variegata lectin genes, respectively, suggesting that the divergence of Erythrina lectins might follow species evolution. This study concluded that ESL could be used to develop lectin-based antimicrobials, which could find applications in the agricultural and health sectors.
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2023, 104, 1; 21-32
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
First Report on Fusarium brachygibbosum Isolate FIR 16_ITS Isolated from Iraqi Wheat Plant
Autorzy:
Ali, Hamdia Z.
Hameed, Mahdi S.
Abdulrahman, Abdulrahman A.
Saood, Hutham M.
Powiązania:
https://bibliotekanauki.pl/articles/123408.pdf
Data publikacji:
2020
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
phylogenetic analysis
rDNA
Iraq
Opis:
In this study, the Fusarium Brachygibbosum fungus isolate FIR 16_ITS was isolated for the first time from the infected wheat plants in Iraq, during the winter of 2018, in the AL-Tewatha region – 20 km south east of Baghdad. In order to verify the identity of the species of the F. brachygibbosum isolate FIR 16_ITS with the growth symptoms on the wheat plants, the wheat seeds and tissue plants were obtained and used as a source for the re-isolation of the infection agent. F. brachygibbosum isolate FIR 16_ITS was hardly identified using visual approach. However, a molecular technique is important in verifying the species of F. brachygibbosum isolate FIR 16_ITS. The isolate obtained was used for the extraction of DNA and later used for the molecular identification and phylogenetic analyses based on rDNA-internal transcribed region (ITS) primer sets. The authors successfully obtained the amplified products of the ITS-rDNA region, and a sequence analysis indicated that the Fusarium sp. isolate FIR 16_ITS species screened in this study belonged to the F. brachygibbosum species. It was recorded in the NCBI under the following information NCBI; BioSample; Accession no.; SAMN11408139; ID: 11408139 and NCBI; BioProject; Accession no.; PRJNA532637; ID: 679434 (Locus Tag Prefix; E7750).
Źródło:
Journal of Ecological Engineering; 2020, 21, 3; 81-86
2299-8993
Pojawia się w:
Journal of Ecological Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Problems, limitations, and challenges in species identifcation of Ascomycota members on the basis of ITS regions
Autorzy:
Baturo-Cieśniewska, Anna
Pusz, Wojciech
Patejuk, Katarzyna
Powiązania:
https://bibliotekanauki.pl/articles/11543330.pdf
Data publikacji:
2020
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
Ascomycota
fungi
internal transcribed spacer
molecular identification
polymerase chain reaction
barcode
rDNA
phylogenetic analysis
Opis:
The internal transcribed spacer (ITS) region is regarded as a formal fungal primary barcode with a high probability of the correct identification for a broad group of fungi. ITS sequences have been widely used to determine many fungal species and analysis of rDNA ITS is still one of the most popular tools used in mycology. However, this region is not equally variable in all groups of fungi; therefore, identification may be problematic and result in ambiguous data, especially in some species-rich genera of Ascomycota. For these reasons, identification based on rDNA ITS is usually complemented by morphological observations and analysis of additional genes. Reliable species identification of Ascomycota members is essential in diagnosing plant diseases, verifying air quality and the effectiveness of agronomic practices, or analyzing relationships between microorganisms. Therefore, the present study aimed to verify, using specific examples, the extent to which ITS sequence analysis is useful in species identification of pathogens and saprobionts from Ascomycota and demonstrate problems related to such identification in practice. We analyzed 105 ITS sequences of isolates originating from air and plant material. Basic local alignment search tool (BLASTn) significantly contributed to the reliable species identification of nearly 80% of isolates such as Arthrinium arundinis, Beauveria bassiana, Boeremia exigua, Cladosporium cladosporioides, Epicoccum nigrum, Nigrospora oryzae, Sclerotinia sclerotiorum, or Sordaria fimicola and members of the genera Alternaria and Trichoderma. However, for most isolates, additional morphological observations, information regarding the isolate origin and, where possible, a PCR with species-specific primers were helpful and complementary. Using our practical approach, we determined that ITS-based species identification and comparative analysis with GenBank sequences significantly helps identifying Ascomycota members. However, in many cases, this should be regarded as suggestive of a taxon because the data usually require the use of additional tools to verify the results of such analysis.
Źródło:
Acta Mycologica; 2020, 55, 1; 5512
0001-625X
2353-074X
Pojawia się w:
Acta Mycologica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Emergence and complete genome of Senecavirus A in pigs of Henan Province in China, 2017
Autorzy:
Wang, H.B.
Tian, B.
Lv, H.L.
Wang, F.
Zhang, T.
Wang, C.Y.
Zhang, Y.D.
Dong, J.J.
Powiązania:
https://bibliotekanauki.pl/articles/2087575.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Senecavirus A
vesicular disease
phylogenetic analysis
Źródło:
Polish Journal of Veterinary Sciences; 2019, 1; 187-190
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Isolation and sequence analysis of the complete VP2 gene of canine parvovirus from Chinese domestic pets and determination of the pathogenesis of these circulating strains in beagles
Autorzy:
Chen, M.R.
Guo, X.Y.
Wang, Z.Y.
Jiang, Y.T.
Yuan, W.F.
Xin, T.
Hou, S.H.
Song, T.Q.
Lin, W.D.
Zhu, H.F.
Jia, H.
Powiązania:
https://bibliotekanauki.pl/articles/2087542.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
canine parvovirus
molecular epidemiology
phylogenetic analysis
pathogenesis
Źródło:
Polish Journal of Veterinary Sciences; 2019, 2; 287-296
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The first detection of the sequence of bacteria from the Simkaniaceae family in surface waters in Poland
Autorzy:
Pawlikowska-Warych, M.
Deptuła, W.
Powiązania:
https://bibliotekanauki.pl/articles/2087599.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Simkaniaceae
surface water
phylogenetic analysis
OdraWCh30 sequence
Polska
Źródło:
Polish Journal of Veterinary Sciences; 2019, 1; 61-65
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
First molecular characterization of Dobrava-Belgrade virus found in Apodemus flavicollis in Poland
Autorzy:
Kolodziej, M.
Melgies, A.
Joniec-Wiechetek, J.
Michalski, A.
Nowakowska, A.
Pitucha, G.
Niemcewicz, M.N.
Powiązania:
https://bibliotekanauki.pl/articles/2081717.pdf
Data publikacji:
2018
Wydawca:
Instytut Medycyny Wsi
Tematy:
Polska
phylogenetic analysis
Apodemus flavicollis
Dobrava-Belgrade virus
orthohantaviruses
Opis:
Introduction. Dobrava-Belgrade virus (DOBV) is one of the emerging pathogens which have been reported during the last decades in Europe and have attracted the attention of researchers. The course of infection among humans may have a varied course – from the completely asymptomatic to the more severe forms, such as haemorrhagic fever with renal syndrome (HFRS). DOBV is hosted and carried by rodents like Apodemus flavicollis or A. agrarius, which occur commonly in Europe. Objective. To-date, orthohantaviruses have been reported in Poland, both in humans and animals, but detailed countryscale studies have not yet been carried out. The aim of the study was molecular characterization of a strain which was found in A. flavicollis in south-eastern Poland. Materials and method. The phylogenetic analysis of the first Dobrava-Belgrade virus found in A. flavicollis in the subcarpathian region of south-eastern Poland, presented in this study, was performed after virus proliferation in cell culture and sequencing of specific PCR products. Results. Based on genetic sequences of fragments of three segments (S, M and L), the isolated virus was assigned to the Dobrava genotype, taking into consideration the most current classification of the DOBV species. Conclusions. The Dobrava-Belgrade virus strain isolated from A. flavicollis in the subcarpathian region of south-eastern Poland, has been molecularly characterized and assigned to Dobrava genotype, thereby the occurrence of that genotype in Poland has been confirmed by molecular techniques.
Źródło:
Annals of Agricultural and Environmental Medicine; 2018, 25, 2; 368-373
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genotyping and pathogenic characterization of canine distemper virus based on mutations in the hemagglutinin gene in Chinese domestic dogs
Autorzy:
Chen, M.
Xin, T.
Hou, S.
Lin, W.
Song, W.
Zhu, H.
Huang, K.
Jia, H.
Powiązania:
https://bibliotekanauki.pl/articles/2087680.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
canine distemper virus
hemagglutinin
phylogenetic analysis
animal infection
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 3; 623-629
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Phylogenetic analysis and genetic structure of new isolates of Tomato mosaic virus in Iran
Autorzy:
Rakhshandehroo, F.
Hashemi, S.S.
Shahraeen, N.
Powiązania:
https://bibliotekanauki.pl/articles/961661.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
phylogenetic analysis
genetic structure
new isolate
tomato mosaic virus
genetic differentiation
genetic variation
phylogenetic tree
Tobamovirus
Iran
Opis:
The present report describes the new occurrence of Tomato mosaic virus (ToMV) in cabbage, bean and Malva neglecta plants in Iran. In this study, sequence analyses of a partial RNA dependent RNA polymerases (RdRp) and complete movement protein (MP) and the coat protein (CP) nucleotide sequences of three new ToMV isolates collected from major crop fields in Iran revealed low genetic variation of RdRp gene compared to the CP and MP genes. The different topologies of the phylogenetic trees constructed, using available open reading frame (ORF1), ORF2 and ORF3 sequences from ToMV isolates, indicated different evolutionary constraints in these genomic regions. Statistical analysis also revealed that with the exception of CP other tested ToMV genes were under negative selection and the RdRp gene was under the strongest constraints. According to the phylogenetic tree it can be inferred from the nucleotide sequences of the complete CP and MP genes, that isolates from Iran and Egypt formed separate groups, irrespective of host origin. However, isolates clustered into groups with correlation to geographic origin but not the host. Analysis of the Ks*, Z* and Snn values also indicated genetic differentiation between ToMV populations. The Tajima’s D, Fu and Li’s statistical values were significantly negative for the RdRp gene of the Asian population which suggests the sudden expansion of ToMV in Asia. Taken together, the results indicate that negative selection and genetic drift were important evolutionary factors driving the genetic diversification of ToMV.
Źródło:
Journal of Plant Protection Research; 2018, 58, 1
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Sequence variants of Yersinia enterocolitica ystB gene detected in wild animals in Poland
Autorzy:
Pieczywek, M.
Bancerz-Kisiel, A.
Szczerba-Turek, A.
Szweda, W.
Powiązania:
https://bibliotekanauki.pl/articles/2087747.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Yersinia enterocolitica
ystB gene
phylogenetic analysis
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 2; 397-399
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular cloning, sequence analysis and developmental stage expression of a putative septin gene fragment from Aedes albopictus (Diptera: Culicidae)
Autorzy:
Avicor, Silas
Wajidi, Mustafa
Jaal, Zairi
Yahaya, Zary
Powiązania:
https://bibliotekanauki.pl/articles/1038803.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Aedes albopictus
cytokinesis
developmental stages
phylogenetic analysis
septin
Opis:
Septins belong to GTPases that are involved in vital cellular activities, including cytokinesis. Although present in many organisms, they are yet to be isolated from Aedes albopictus. This study reports for the first time on a serendipitous isolation of a partial septin sequence from Ae. albopictus and its developmental expression profile. The Ae. albopictus partial septin sequence contains 591 nucleotides encoding 197 amino acids. It shares homology with several insect septin genes and has a close phylogenetic relationship with Aedes aegypti and Culex quinquefasciatus septins. The Ae. albopictus septin fragment was differentially expressed in the mosquito's developmental stages, with an increased expression in the adults.
Źródło:
Acta Biochimica Polonica; 2016, 63, 2; 243-246
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular identification of pathogenic Fusarium species, the causal agents of tomato wilt in western Iran
Autorzy:
Chehri, K.
Powiązania:
https://bibliotekanauki.pl/articles/66185.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
molecular identification
pathogen
Fusarium
causal agent
tomato
Lycopersicon esculentum
wilt pathogen
pathogenicity
phylogenetic analysis
Iran
Opis:
Fusarium species are causal agents of fungal diseases occurring frequently in numerous agriculturally important plants, including potato, garlic and are one of the common pathogens of tomato, causing root rot in the west part of Iran. Therefore, the objectives of this study were to isolate and identify disease-causing Fusarium species from infected tomatoes based on the morphological and molecular characteristics. Twenty-five isolates of Fusarium were obtained from infected root of tomato plants collected from the fields in different regions of western Iran. Based on morphological features, the strains were classified into four following Fusarium species: F. oxysporum, F. redolens, F. proliferatum and F. verticillioides. The phylogenetic trees based on tef1 and tub2 dataset clearly distinguished closely related species. All of the isolates were evaluated for their pathogenicity on healthy tomato seedlings in the greenhouse. This is the first report on molecular identification of Fusarium species isolated from tomato plants cultivated in Iran.
Źródło:
Journal of Plant Protection Research; 2016, 56, 2
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Phylogenetic analyses of Bartonella sp. (Bacteria) of rodents from four separated valleys in Sinai Mountains (Egypt)
Autorzy:
Alsarraf, M.
Mohallal, E.M.
Kwiatowski, J.
Mierzejewska, E.J.
Welc-Faleciak, R.
Behnke, J.M.
Bajer, A.
Powiązania:
https://bibliotekanauki.pl/articles/6477.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
phylogenetic analysis
Bartonella
bacteria
rodent
valley
Sinai Mount
Egypt
Źródło:
Annals of Parasitology; 2016, 62, Suppl.
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prevalence and phylogenetic analysis of Fig mosaic virus and Fig badnavirus-1 in Iran
Autorzy:
Alimoradian, M.
Rakhshandehroo, F.
Shams-bakhsh, M.
Powiązania:
https://bibliotekanauki.pl/articles/66369.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
prevalence
phylogenetic analysis
Fig mosaic virus
Fig badnavirus-1
Iran
fig tree
plant disease
Opis:
Fig mosaic virus (FMV) and Fig badnavirus-1 (FBV-1) are two of the most important fig infecting viruses. The incidence and distribution of FBV-1 and FMV were determined by testing in PCR 138 asymptomatic and symptomatic samples. These samples were collected from 60 fig gardens and agricultural fields in three provinces of Iran. The fig infecting viruses FBV-1 and FMV, respectively, were detected in 92 (66.6%) and 34 (24.6%) samples collected from all the surveyed fields. Overall, 24 out of 138 (17.3%) samples showed mixed infections. The sequence analysis of a genomic fragment of 922 nt, comprising the entire ORF-2 and part of the 5’ termini of the ORF-3 of 10 selected FBV-1 Iranian isolates from different provinces, and of the type member from GenBank (Acc. No: JF411989), showed a variation ranging from 1 to 3% at nucleotide level and 1% at the amino acid level. The phylogenetic analysis grouped the FBV-1 isolates into two groups, with the Iranian isolates clustered in two distinct subgroups of group I, according to their geographical origin. In our research, the prevalence and sequence analysis of FBV-1 as the only identified DNA virus infecting fig trees, was studied for the first time in Iran.
Źródło:
Journal of Plant Protection Research; 2016, 56, 2
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Morphological and Morphogenetic Redescriptions and SSU rRNA Gene-based Phylogeny of the Poorly-known Species Euplotes amieti Dragesco, 1970 (Ciliophora, Euplotida)
Autorzy:
LIU, Mingjian
FAN, Yangbo
MIAO, Miao
Hu, Xiaozhong
Al-Rasheid, Khaled A. S.
Al-Farraj, Saleh A.
Ma, Honggang
Powiązania:
https://bibliotekanauki.pl/articles/763760.pdf
Data publikacji:
2015
Wydawca:
Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
Tematy:
Euplotidae, fresh water ciliate, ontogenesis, phylogenetic analysis, taxonomy
Opis:
This paper investigates the morphology and morphogenesis during binary fission of a Chinese population of Euplotes amieti Dragesco, 1970, a fresh water form which has previously not been well defined. This organism is morphologically very similar to the well-known Euplotes eurystomus but differs from the latter both in the number of dorsal kineties and the molecular data. According to the information obtained, it is characterized by a combination of features including nine frontoventral cirri, ca. 60 membranelles, 12–15 dorsal kineties, a macronucleus in the shape of the number 3, and a ‘double-eurystomus’ type of silverline system. Its morphogenesis proceeds broadly in the same pattern as in its congeners. In this study, the SSU rRNA gene was sequenced for the first time, and phylogenetic analyses indicated that it is closely related to the eurystomus-aediculatus-woodruffi- complex. Considering the extreme similarities in morphology between E. amieti and E.eurystomus, we believe that the four sequences (four isolates) under the name of Euplotes eurystomus (No. FR873716; FR873717; EF193250; AJ310491 deposited in GenBank) are very likely from misidentified material; that is, they represent different populations of Euplotes amieti.
Źródło:
Acta Protozoologica; 2015, 54, 3
1689-0027
Pojawia się w:
Acta Protozoologica
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 40

Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies