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Wyszukujesz frazę "phenylalanine-ammonia lyase" wg kryterium: Temat


Wyświetlanie 1-9 z 9
Tytuł:
High performance liquid chromatography and photodiode array detection of ferulic acid in Rubus protoplasts elicited by O-glycans from Fusarium sp. M7-1.
Autorzy:
Nita-Lazar, Mihai
Chevolot, Lionel
Iwahara, Shojiro
Takegawa, Kaoru
Furmanek, Aleksandra
Lienart, Yvette
Powiązania:
https://bibliotekanauki.pl/articles/1043710.pdf
Data publikacji:
2002
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
phenylalanine-ammonia lyase
Rubus protoplasts
Fusarium glycoproteins
O-glycan signal
Opis:
So far only little data have been available concerning the eliciting capacity of well defined glycan molecules isolated from plant pathogens. This study brings new information about changes in plant cells caused by fungal pathogens. Sugar fractions derived from glycoproteins isolated from the fungus Fusarium sp. M7-1 have been tested here as signaling molecules. The ability of three O-glycan fractions (named in this work inducer I, II, III) to trigger responses in Rubus protoplasts has been examined. It was found that inducer III was the most efficient as it elicited changes in the levels of phenylpropanoid pathway intermediates in relation to phenylalanine-ammonia lyase (PAL) activation.
Źródło:
Acta Biochimica Polonica; 2002, 49, 4; 1019-1027
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Novel oligosaccharides isolated from Fusarium oxysporum L. rapidly induce PAL activity in Rubus cells.
Autorzy:
Nita-Lazar, Mihai
Heyraud, Alain
Gey, Claude
Braccini, Isabelle
Lienart, Yvette
Powiązania:
https://bibliotekanauki.pl/articles/1041538.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
oligosaccharide elicitors
Rubus fructicosus
phenylalanine ammonia lyase
Fusarium oxysporum
Opis:
Activation of the phenolic pathway is known to be part of a defense response against cell wall-derived elicitors from pathogens. Many examples of a defense response by increasing the synthesis of phenolic compound against the elicitor were demonstrated in the past, but the elicitor structure has so far been poorly characterized. Our results indicate that a disaccharide fraction containing the following structure: α-D-mannopyranosyl (1→2)α/β-D-glucopyranosyl and α-D-mannopyranosyl (1→x) inositol, isolated from Fusarium oxysporum L., promotes rapid and transient phenylalanine ammonia lyase activity in Rubus fructicosus cells at nanomolar concentration. The disaccharides were isolated by size-exclusion chromatography directly from extracts obtained by alkaline treatment of F. oxysporum mycelium. Their structure was determined by 500-MHz-1H-NMR spectroscopy combined with methylation analysis and fast atom bombardment mass spectrometry.
Źródło:
Acta Biochimica Polonica; 2004, 51, 3; 625-634
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Activation of phenylpropanoid pathway in legume plants exposed to heavy metals. Part I. Effects of cadmium and lead on phenylalanine ammonia-lyase gene expression, enzyme activity and lignin content
Autorzy:
Pawlak-Sprada, Sylwia
Arasimowicz-Jelonek, Magdalena
Podgórska, Magdalena
Deckert, Joanna
Powiązania:
https://bibliotekanauki.pl/articles/1039918.pdf
Data publikacji:
2011
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
cadmium
gene expression
lead
lignin
lupine
phenylalanine ammonia-lyase (PAL)
soybean
Opis:
Species-specific changes in expression of phenylalanine ammonia-lyase (PAL) and lignin content were detected in roots of soybean (Glycine max L.) and lupine (Lupinus luteus L.) seedlings treated with different concentrations of cadmium (Cd2+, 0-25 mg/l) or lead (Pb2+, 0-350 mg/l). The stimulatory effect of both metals was observed in mRNA coding for PAL in soybean. In the case of lupine, changes of PAL mRNA level were dependent on the metal used: Cd2+ caused a decrease, whereas Pb2+ an increase of PAL transcript level. The activity of PAL was enhanced in both plant species at higher metal concentrations (15-25 mg/l of Cd2+ or 150-350 mg/l of Pb2+); however it was not directly correlated with PAL mRNA. This suggests a transcriptional and posttranscriptional control of PAL expression under heavy metals stress. In soybean, Cd2+ or Pb2+ treatment increased lignin content, while in lupine the effect was opposite. The decreased lignin accumulation in lupine roots in response to heavy metals, despite an increased PAL activity, suggests that the activated phenylpropanoid pathway was involved in the synthesis of secondary metabolites other than lignin.
Źródło:
Acta Biochimica Polonica; 2011, 58, 2; 211-216
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Polish strains of Trichoderma in cucumber plant growth promotion and induction of defence reaction against Rhizoctonia solani
Autorzy:
Nawrocka, J.
Malolepsza, U.
Szczech, M.
Powiązania:
https://bibliotekanauki.pl/articles/81297.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
Polish strain
Trichoderma
cucumber
Cucumis sativus
plant growth
biomass production
infection
Rhizoctonia solani
phenylalanine ammonia lyase
total phenol
defense reaction
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The influence of BTH and arbutin on PAL activity and phenolic compounds content in cucumber leaves infected with Pseudomonas syringae pv. lachrymans
Autorzy:
Sklodowska, M.
Wielanek, M.
Gajewska, E.
Kuzniak, E.
Powiązania:
https://bibliotekanauki.pl/articles/81241.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
benzo[1,2,3]thiadiazole-7-carbothioic acid S-methyl ester
phenylalanine ammonia lyase
phenolic compound content
flavonoids content
arbutin
leaf spot
Pseudomonas syringae pv.lachrymans
cucumber leaf
infection
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Characterization of the phenylalanine ammonia lyase gene from the rubber tree (Hevea brasiliensis Müll. Arg.) and differential response during Rigidoporus microporus infection
Autorzy:
Sangsil, P.
Nualsri, C.
Woraathasin, N.
Nakkanong, K.
Powiązania:
https://bibliotekanauki.pl/articles/66099.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
phenylalanine ammonia lyase
gene expression
rubber
tree
Hevea brasiliensis
plant response
Rigidoporus microporus
plant infection
root rot disease
Opis:
Phenylalanine ammonia lyase (PAL) is a specific branch point enzyme of primary and secondary metabolism. It plays a key role in plant development and defense mechanisms. Phenylalanine ammonia lyase from Hevea brasiliensis (HbPAL) presented a complete open reading frame (ORF) of 2,145 bp with 721 encoded amino acids. The sequence alignment indicated that the amino acid sequence of HbPAL shared a high identity with PAL genes found in other plants. Phylogenetic tree analysis indicated that HbPAL was more closely related to PALs in Manihot esculenta and Jatropha curcas than to those from other plants. Transcription pattern analysis indicated that HbPAL was constitutively expressed in all tissues examined, most highly in young leaves. The HbPAL gene was evaluated by quantitative real-time PCR (qRT-PCR) after infection with Rigidoporus microporus at 0, 12, 24, 48, 72 and 96 hours post inoculation. The expression patterns of the PAL gene differed among the three rubber clones used in the study. The transcription level of the white root rot disease tolerant clone, PB5/51 increased sharply during the latter stages of infection, while it was relatively subdued in the white root rot disease susceptible clones, RRIM600 and BPM24. These results suggest that the HbPAL gene may play a role in the molecular defense response of H. brasiliensis to pathogen attack and could be used as a selection criterion for disease tolerance.
Źródło:
Journal of Plant Protection Research; 2016, 56, 4
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of Drechslera graminea on total soluble proteins and defense enzymes of barley
Autorzy:
Goel, N.
Jaiswal, G.
Srivastava, A.K.
Paul, P.K.
Goel, A.
Powiązania:
https://bibliotekanauki.pl/articles/66670.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
barley
Hordeum vulgare
defense enzyme
Drechslera graminea
peroxidase
phenylalanine lyase
polyphenol oxidase
protein
soluble protein
tyrosine ammonia lyase
Opis:
Barley phylloplane is seriously colonized by Drechslera graminea, the causal agent of leaf stripe disease in the hos. The present study involved the elucidation of alterations induced in the protein content of the host due to Drechslera infection. Naturally growing barley plants were obtained from fields and Drechslera graminea was isolated and identified from diseased plants’ leaves. After identification and preparation of the pure culture, the pathogen was inoculated on plants grown under aseptic and controlled laboratory conditions. Changes in the total soluble cytoplasmic proteins and defense enzymes of the host such as polyphenol oxidase (PPO), peroxidase (POX), phenylalanine lyase (PAL) and tyrosine ammonia lyase (TAL) were observed up to 5 h after inoculation. The results demonstrated a significant effect of the pathogen on the cytoplasmic protein expression of the host as well as in its defense system.
Źródło:
Journal of Plant Protection Research; 2018, 58, 3
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Aspergillus niger, a dominant phylloplane coloniser, influences the activity of defense enzymes in Solanum lycopersicum
Autorzy:
Goswami, S.
Paul, P.K.
Sharma, P.D.
Powiązania:
https://bibliotekanauki.pl/articles/2084798.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Aspergillus niger
phylloplane
peroxidase
phenylalanine ammonia lyase
polyphenol oxidase
Solanum lycopersicum
tyrosine ammonia lyase
Opis:
Phylloplane microbes have been studied as strategic tools in management against plant pathogens. Non-pathogenic bacteria and fungi have been applied as crop protectants against various plant diseases. The present study aimed at evaluating the potentiality of Aspergillus niger spores in altering the activity of four key enzymes related to defense in tomato. The experiment was designed such that two groups of 50 tomato plants were considered: group 1 – sprayed with autoclaved distilled water (control) and group 2 – sprayed with A. niger spores. Spraying was carried out under aseptic conditions. The experimental parameters included analysis of the activity of peroxidase (POX), polyphenol oxidase (PPO), phenylalanine ammonia lyase (PAL) and tyrosine ammonia lyase (TAL) as well as expression of POX and PPO isoforms. The results demonstrated an inductive effect of A. niger on the activity of POX, PPO, PAL and TAL. Enhanced expression of POX and PPO isoforms was also observed. The results indicated that A. niger can be considered probiotic for the management of tomato against its phytopathogens.
Źródło:
Journal of Plant Protection Research; 2019, 59, 4; 512-518
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Higher induction of defense enzymes and cell wall reinforcement in maize by root associated bacteria for better protection against Aspergillus niger
Autorzy:
Jha, Y.
Powiązania:
https://bibliotekanauki.pl/articles/2084643.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Aspergillus niger
β-1
3-glucanase
biotic factor
phenylalanine ammonia lyase
RAPD analysis
rhizosphere
root associated bacteria
Opis:
Root associated bacteria were isolated from Suaeda nudiflora and two isolates were selected for this study: rhizospheric Bacillus megaterium and endophytic Pseudomonas aeruginosa. These isolates were inoculated into maize variety Narmada Moti during its germination. TTC (2, 3, 5-triphenyl tetrazolium chloride) staining was used to confirm the association of the isolates with the maize root. The effects of these root associated bacteria were tested alone and in combinations for cell wall reinforcement and the induction of defense enzymes such as phenylalanine ammonia lyase (PAL) and β-1,3-glucanase in the presence of fungal pathogen Aspergillus niger in maize. The results indicated that the rhizospheric bacteria had a greater fight response to fungal infection than the endophhytic bacteria due to cell wall lignification as well as the rapid induction of higher concentrations of defense related enzymes.
Źródło:
Journal of Plant Protection Research; 2019, 59, 3; 341-349
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-9 z 9

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