Temat: peripheral blood - Katalog OPAC zbiorów

Tytuł:: Spectral properties of phthalocyanines incorporated into resting and stimulated human peripheral blood cells.
Autorzy:: Waszkowiak, Aneta
Frąckowiak, Danuta
Wiktorowicz, Krzysztof
Miyake, Jun
Powiązania:: https://bibliotekanauki.pl/articles/1043724.pdf
Data publikacji:: 2002
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: phthalocyanines
fluorescence
photodynamic therapy
human peripheral blood cells
Opis:: Human peripheral blood cells stimulated by phytohemagglutinin (which serve as a model of cancerous cells) and resting cells were incubated in dimethyl sulfoxide solutions of various phthalocyanines. In order to diminish the influence of atmospheric oxygen the cells were embedded in a polymer (polyvinyl alcohol) film. Fluorescence spectra of the samples were measured over two regions of excitation wavelengths: at 405 nm (predominant absorption of the cell material) and in the regions of strong absorption of phthalocyanines (at about 605 nm and 337 nm). The intrinsic emission of cell material became changed as a result both of cells' stimulation and of incubation of cells in dye solution. In most cases the stimulated cells when stained by dye exhibited higher long wavelength fluorescence intensity than resting cells. This suggests higher efficiency of dye incorporation into cancerous cells than into healthy cells. The absorption spectra of samples were also measured. The spectra of various phthalocyanines in incubation solvent, in polymer and in the cells embedded in polymer, were compared. The comparison of properties of the cells stimulated for different time periods enabled to establish the conditions of stimulation creating a population of cells incorporating a large number of sensitizing molecules.
Źródło:: Acta Biochimica Polonica; 2002, 49, 3; 633-641
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 9.

Tytuł:: Evaluation of DNA damage in white blood cells of healthy human volunteers using the alkaline comet assay and the chromosome aberration test
Autorzy:: Kopjar, Nevenka
Želježić, Davor
Garaj-Vrhovac, Verica
Powiązania:: https://bibliotekanauki.pl/articles/1041246.pdf
Data publikacji:: 2006
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: peripheral blood
chromosome aberration test
white blood cells
DNA damage
alkaline comet assay
lymphocytes
Opis:: The present study was undertaken to contribute to the characterization of the degree of variability in baseline damage in white blood cells from control population, and to investigate how this variability is associated with external and internal factors. Altogether 170 healthy volunteers, randomly selected from the general population of the Republic of Croatia, participated in the study. Two sensitive tests: the alkaline comet assay and the chromosome aberration test were applied to study the background levels of DNA damage in their white blood cells. The results point to inter-individual differences, indicating different genome sensitivity. As revealed by both assays, the background levels of DNA damage were mostly influenced by smoking habit as well as medical exposure (especially to diagnostic X-rays). Sex and age of subjects did not significantly influence the values of DNA damage recorded in the white blood cells. Although higher levels of DNA damage were recorded in blood samples collected during winter and autumn, they were mostly influenced by medicinal exposure and smoking habit. Statistical evaluation of the data confirmed that a positive correlation exists between DNA migration and the number of long-tailed nuclei found with the comet assay and the total number of chromosome aberrations. The data obtained can serve as control values in forthcoming biomonitoring studies.
Źródło:: Acta Biochimica Polonica; 2006, 53, 2; 321-336
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 10.

Tytuł:: Ocena czestosci wystepowania mikrojader w erytrocytach myszy eksponowanych subchronicznie na promieniowanie jonizujace i nonylofenol
Frequency of micronuclei in erythrocytes of mice subchronic exposed to ionising radiation and nonylphenol
Autorzy:: Dobrzynska, M M
Powiązania:: https://bibliotekanauki.pl/articles/871848.pdf
Data publikacji:: 2008
Wydawca:: Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny
Tematy:: zwierzeta laboratoryjne
myszy
promieniowanie jonizujace
nonylofenol
krew obwodowa
szpik kostny
mikrojadra
retikulocyty
erytrocyty polichromatyczne
laboratory animal
mouse
ionizing radiation
nonylphenol
peripheral blood
bone marrow
micronucleus
reticulocyte
polychromatic erythrocyte
Opis:: Celem pracy było określenie indukcji mikrojąder w retikulocytach i erytrocytach polichromatycznych myszy pod wpływem subchronicznej ekspozycji na promieniowanie X, nonylofenol (NF) lub na skojarzone działanie obu czynników. Myszy Pzh: SFIS narażane były przez 8 tygodni, 5 razy w tygodniu. Dawki wynosiły 0.05 Gy lub 0,10 Gy promieniowania X, 25 mg/kg mc lub 50 mg/kg mc nonylofenolu, a w przypadku skojarzonego działania 0,05 Gy + 25 mg/kg mc NF lub 0,10 Gy + 50 mg/kg mc NF. Zarówno promieniowania X, jak i NF działając oddzielnie indukowały powstawanie mikrojąder w retikulocytach i erytrocytach polichromatycznych myszy. Skojarzone działanie obu czynników w retikulocytach krwi obwodowej oraz w erytrocytach polichromatycznych szpiku kostnego indukowało zwiększoną częstość występowania mikrojąder w porównaniu do efektów działania każdego z czynników oddzielnie. W retikulocytach szpiku kostnego skojarzone działanie obu czynników w małych dawkach (0,05 Gy + 50 mg/kg mc NF) powodowało zwiększenie efektu mutagennego. Natomiast, w przypadku skojarzenia obu czynników w większych dawkach (0,10 Gy + 50 mg/kg mc NF), nonylofenol może wywierać efekt ochronny w stosunku do DNA retikulocytów powodując zmniejszenie uszkodzeń indukowanych przez promieniowanie X.
The aim of the research was to investigate the level of micronuclei induction in reticulocytes and polychromatic erythrocytes of mice following subchronic exposure to X-rays, nonylphenol (NP) or to a combination of both. Male mice Pzh:SFIS were exposed during 8 weeks, 5 day per week to doses 0.05 Gy and 0.10 Gy of X-rays, 25 mg/kg mc and 50 mg/kg bw of nonylphenol as well as to 0.05 Gy + 25 mg/kg bw NP and 0.10 Gy + 50 mg/kg bw NP for combined exposure. Both X-rays and NP, acting alone induced micronuclei in reticulocytes and in polychromatic erythrocytes of mice. Combined X-rays-NP exposure of peripheral blood reticulocytes and bone marrow polychromatic erythrocytes caused enhanced frequency of micronuclei compared to the effect of each agent alone. In bone marrow reticulocytes, combined exposure in lower doses (0.05 Gy + 25 mg/kg bw NP) induced enhanced mutagenic effect. Contrary, after combined exposure to both agents in higher doses (0.10 Gy + 50 mg/kg bw NP), nonylphenol may protected DNA of reticulocytes against damage induced by X-rays.
Źródło:: Roczniki Państwowego Zakładu Higieny; 2008, 59, 3; 309-318
0035-7715
Pojawia się w:: Roczniki Państwowego Zakładu Higieny
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 11.

Tytuł:: Contact allergy to nickel: Patch test score correlates with IL-5, but not with IFN-gamma nickel-specific secretion by peripheral blood lymphocytes
Autorzy:: Czarnobilska, E
Jenner, B.
Kaszuba-Zwoinska, J.
Kapusta, M.
Obtulowicz, K.
Thor, P.
Spiewak, R.
Powiązania:: https://bibliotekanauki.pl/articles/49735.pdf
Data publikacji:: 2009
Wydawca:: Instytut Medycyny Wsi
Tematy:: allergic contact dermatitis
patch test
contact allergy
lymphocyte
interleukin 5
interferon gamma
risk estimation
nickel
peripheral blood
chronic disease
Źródło:: Annals of Agricultural and Environmental Medicine; 2009, 16, 1; 37-41
1232-1966
Pojawia się w:: Annals of Agricultural and Environmental Medicine
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 12.

Tytuł:: Effect of Biolex Beta-HP on phagocytic activity and oxidative metabolism of peripherial blood granulocytes and monocytes in rats intoxicated by cyclophosphamide
Autorzy:: Wojcik, R
Powiązania:: https://bibliotekanauki.pl/articles/32265.pdf
Data publikacji:: 2010
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: beta-1,3-1,6-D-glucan
cyclophosphamide
rat
phagocytic activity
oxidative metabolism
granulocyte
monocyte
flow cytometry
peripheral blood
immunosuppression
blood neutrophil
Opis:: The objective of this study was to determine the effect of ß-l,3/l,6-D-glucan (Biolex-Beta HP) on the phagocytic activity and oxidative metabolism of peripheral blood granulocytes and monocytes in rats intoxicated by cyclophosphamide. The experimental material comprised 40 adult Wistar rats aged 14 weeks, divided into two equal groups, a control group and an experimental group, each of 20 adult rats, including 10 males and 10 females. In the course of 3 successive days, 20 rats from the experimental group were administered cyclophosphamide intramuscularly at a dose of 50 mg/kg body weight/day. On the 8th day of the experiment, 10 control group (K) rats and 10 experimental group (C) rats were sacrificed. Arterial blood samples were collected and diluted with heparin to determine and compare the phagocytic activity (Phatogest) and oxidative metabolism (Bursttest) of peripheral blood granulocytes and monocytes by flow cytometry. Starting on the 8lh day of the experiment, the feed of the remaining 10 rats from the experimental group (C+G) and 10 rats from the control group (G) was supplemented with Biolex-Beta HP at a dose of 50 mg/kg body weight/day for 14 consecutive days. On day 22, arterial blood samples were collected from all (C+G) and (C) group rats, diluted with heparin to determine and compare the phagocytic activity and oxidative metabolism of peripheral blood granulocytes and monocytes by flow cytometry. The results showed that Biolex-Beta HP modulated the phagocytic activity and oxidative metabolism of blood neutrophils and monocytes suppressed by cyclophosphamide in rats. The immunorestoring activity of Biolex-Beta HP was observed in this study.
Źródło:: Polish Journal of Veterinary Sciences; 2010, 13, 1; 181-188
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 13.

Tytuł:: Evaluation of the influence of meloxicam and flunixin meglumine on the apoptosis of peripheral blood CD4plus and CD8plus T cells in calves
Autorzy:: Maslanka, T
Jaroszewski, J.J.
Markiewicz, W.
Jabubowski, P.
Powiązania:: https://bibliotekanauki.pl/articles/31556.pdf
Data publikacji:: 2010
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: peripheral blood
T cell
calf
CD4plus T cell
CD8plus T cell
apoptosis
meloxicam
flunixin meglumine
lymphocyte
antiinflammatory drug
Opis:: The aim of the study was to determine whether treatment with recommended doses of meloxicam or flunixin had an effect on the apoptosis of peripheral blood T lymphocytes in calves. The study was carried out on 4-5 months old calves (n = 24, 8 per group). Experimental animals were injected subcutaneously with a single dose of 0.5 mg . kg-1 of meloxicam or intravenously with 3 doses of 2.2 mg . kg-1 day-1 of flunixin. The non-treatment animals served as control. Blood samples were taken at day 0 and at days 1, 2, 3, 5, 7 and 14 after the first NSAIDs injection. Apoptosis was determined by flow cytometry using Annexin V-PE/7-AAD staining. The kinetic analysis of apoptosis in the total lymphocyte population, as well as in the CD4+ and CD8+ subsets did not reveal significant differences in the frequency of early apoptotic cells between control and experimental groups throughout the period studied. Although, 24 h after administration of the first dose of NSAIDs, late-stage apoptosis/necrosis was significantly increased in the total lymphocyte population (the meloxicam group), as well as in the CD4+ (the meloxicam group and the flunixin group) and CD8+ (the flunixin group) subsets of T cells. However, this disturbance was transient, relatively poorly expressed and, thus, unlikely to be of clinical significance. Our results indicate that the use of meloxicam or flunixin in accordance with the recommended dosage regimen in cattle do not have a clinically significant influence on apoptosis of peripheral blood T cells.
Źródło:: Polish Journal of Veterinary Sciences; 2010, 13, 1; 3-12
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 14.

Tytuł:: Response of olfactory receptors and blood cells of Lake Baikal fish to phenol exposure
Autorzy:: Yakhnenko, V.
Klimenkov, I.
Pastukhova, N.
Powiązania:: https://bibliotekanauki.pl/articles/363284.pdf
Data publikacji:: 2010
Wydawca:: Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:: komórka
ryby endemiczne
system immunologiczny
ryby nieendemiczne
nabłonek węchowy
krew obwodowa
cells
endemic fish
immune system
nonendemic fish
olfactory epithelium
peripheral blood
Opis:: Exposure to phenol causes ultrastructural disorders in supporting and receptor cells in the peripheral segment of the olfactory organ of yellowfin (Cottocomephorus grewingkii) and in cells in peripheral blood of stone sculpins (Paracottus knerii), yellowfin and perch (Perca fluviatilis). Identical responses to phenol exposure in the blood cells were observed in endemic Lake Baikal fish and in nonendemic fish. Differences were found only in the rate and intensity of the recovery response. Fast activation of immune processes were found in the coastal nonendemic perch, but they were slow in the stone sculpin, a coastal endemic species.
Źródło:: Environmental Biotechnology; 2010, 6, 2; 66-73
1734-4964
Pojawia się w:: Environmental Biotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 15.

Tytuł:: Cellular immune response of pigeons in the conditions of endotoxin fever and pyrogenic tolerance
Autorzy:: Dudek, K.
Bednarek, D.
Powiązania:: https://bibliotekanauki.pl/articles/30231.pdf
Data publikacji:: 2011
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: cellular immune response
endotoxin fever
fever
pigeon
pyrogenic tolerance
bird
Escherichia coli
defense mechanism
bacteria
Salmonella
Pseudomonas
peripheral blood
immunophenotyping
Opis:: The aim of this study was to investigate changes in selected parameters of cellular immune response in the conditions of endotoxin fever and pyrogenic tolerance in pigeons. On the first day of observation the experimental birds (n=18) were intravenously injected with Escherichia coli LPS at a dose of 10 μg/kg b.w., while the control animals (n=6) received apyrogenic physiological saline also in the form of injection. On the second and the third day of the experiment LPS was injected additionally at 24 h intervals. Four and a half hours after the saline and pyrogen administration blood samples were collected from the control and experimental pigeons. The following immunological assays were performed: WBC, leucogram and immunophenotyping of lymphocyte subsets in peripheral blood, i.e. CD 3+ (T lymphocytes), CD 4+ (T helper lymphocytes) and CD 8+ (T suppressor/ cytotoxic lymphocytes) cells. In the conditions of endotoxin fever (i.e. after the first LPS injection) leucopenia, monocytopenia, heterophilia and eosinophilia were observed. Additionally, the immunophenotyping of peripheral blood lymphocytes indicated an increase in percentage of CD 3+, CD 4+ and CD 8+ cells in response to the single injection of LPS. In contrast, the consecutive injections of LPS, which created a pyrogenic tolerance effect, caused a decrease in WBC value, heteropenia, eosinopenia and lymphocytosis. Moreover, during this state an increase in percentage of CD 3+ and CD 8+ cells was demonstrated in contrast to the percentage of CD 4+ lymphocytes. The general tendencies in cellular immune response of the affected pigeons in the conditions of endotoxin fever and pyrogenic tolerance aim at activation of defence mechanisms against LPS for its prompt elimination from the animal’s organism.
Źródło:: Polish Journal of Veterinary Sciences; 2011, 14, 1
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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