Temat: peripheral blood - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: A study on influence of different signs of air-ions on sister-chromatid exchange frequency and chromosome aberration in human peripheral blood lymphocytes
Autorzy:: Wiszniewski, A
Kretowicz, T.
Powiązania:: https://bibliotekanauki.pl/articles/2043653.pdf
Data publikacji:: 1999
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: blood lymphocyte
sister-chromatid exchange
man
peripheral blood lymphocyte
lymphocyte
chromosome aberration
Źródło:: Journal of Applied Genetics; 1999, 40, 2; 129-134
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Automatyczna klasyfikacja komórek rozmazu krwi obwodowej na przykładzie zatrucia ołowiem
Automatic classification of peripheral blood smear cells by the example of lead poisoning
Autorzy:: Michalski, Adrian
Kupcewicz, Bogumiła
Powiązania:: https://bibliotekanauki.pl/articles/762603.pdf
Data publikacji:: 2020-07-28
Wydawca:: Polskie Towarzystwo Farmaceutyczne
Tematy:: uczenie maszynowe
analiza rozmazu krwi obwodowej
klasyfikacja białych krwinek
przetwarzanie obrazu
Image Processing
machine learning
peripheral blood smear analysis
leukocyte classification
Opis:: The first symptoms caused by heavy metals poisoning are usually non-specific, therefore their diagnosis requires specialized knowledge and experience. Incorrect diagnosis can lead to various disorders and irreversible changes in patient’s health. Lead poisoning is one of the heavy metals poisoning which is associated with non-specific symptoms and may cause a broad range of biochemical, physiological and behavioral disfunctions. Lead is commonly found in industry and manufacturing and in 2017, lead poisoning caused over a million deaths worldwide. Due to non-specific symptoms, lead poisoning is often diagnosed too late or incorrectly. Early symptoms are headache and stomachache, which potentiate with metal concentration. Lead poisoning increasing risk of anemia due to inhibition the ability to produce hemoglobin by interfering the heme biosynthesis pathway and decreasing red blood cell survival. Because of regularly occurred disorders of hematopoiesis and the presence of atypical cell in smears, diagnosis of lead poisoning that gives relevant information is bone marrow and peripheral blood smear study. The manual smear test made by qualified technician involves the evaluation of the preparation by using an optical microscope. Manual microscopic examination of blood cells is time-consuming and subjective. Automation of this process would enable to reduce the risk of human failure and solve the problem with lack of professional staff. Traditional machine learning is the most popular approach to automating microscopic smear testing. This method consists of the following stages: microscopic image acquisition, preprocessing, cell segmentation, feature extraction, followed by classification into types, artifacts and atypical cells. This work presents the recent methods proposed to automate the analysis of peripheral blood and bone marrow smears using traditional machine learning methods. A review of different machine learning methods was carried out, focusing on the presentation of an algorithm for the construction of automatic blood cell classifiers.
Pierwsze objawy pacjenta po zatruciu metalami ciężkimi są najczęściej nieswoiste, dlatego ich diagnostyka jest zadaniem wymagającym specjalistycznej wiedzy i doświadczenia. Nieprawidłowa diagnoza może prowadzić do nieodwracalnych zmian w stanie zdrowia pacjenta. Jednym z metali ciężkich, którym zatrucie wiąże się z nieswoistymi objawami, a może prowadzić do poważnych zaburzeń neurologicznych i hematologicznych jest ołów. Ołów jest metalem ciężkim powszechnie występującym w przemyśle i produkcji. W 2017 roku zatrucie tym metalem było przyczyną ponad miliona zgonów na świecie. Z powodu nieswoistych objawów zatrucie ołowiem często jest diagnozowane nieprawidłowo i zbyt późno. Zatrucie metalami ciężkimi może prowadzić do zaburzeń hematopoezy i pojawienia się w rozmazach krwi i szpiku kostnego komórek atypowych, w związku z czym badaniem niosącym istotną informację w diagnostyce zatrucia jest ocena komórek w rozmazach. Analiza rozmazu manualnego polega na ocenie preparatu przy użyciu mikroskopu optycznego oraz szczegółowej interpretacji obrazu mikroskopowego wykonywanej przez wykwalifikowany personel. Automatyzacja tego procesu pozwoliłaby na ograniczenie ryzyka pomyłki spowodowanej błędem ludzkim oraz rozwiązanie problemu braku doświadczonego personelu. Najpopularniejszym podejściem wykorzystywanym w automatyzacji mikroskopowego badania rozmazu jest tradycyjne uczenie maszynowe. Metoda ta, składa się z kilku etapów: zbieranie obrazów mikroskopowych i ich wstępna obróbka, wyodrębnienie komórek oraz ich charakterystycznych cech, a następnie klasyfikacja na rodzaje, artefakty i komórki atypowe. W pracy opisano aktualnie proponowane sposoby automatyzacji badania rozmazu krwi obwodowej i szpiku kostnego, wykorzystujące tradycyjne metody uczenia maszynowego. Przeprowadzono przegląd metod uczenia maszynowego, skupiając się na przedstawieniu algorytmu postępowania w konstrukcji automatycznych klasyfikatorów komórek krwi.
Źródło:: Farmacja Polska; 2020, 76, 6; 318-323
0014-8261
2544-8552
Pojawia się w:: Farmacja Polska
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Can the leukocyte`s parameters in peripheral blood smear predict the risk of in-hospital death of patients undergoing high-risk gastrointestinal surgery?
Autorzy:: Pluta, Michał
Krzych, Łukasz
Powiązania:: https://bibliotekanauki.pl/articles/1392359.pdf
Data publikacji:: 2019
Wydawca:: Index Copernicus International
Tematy:: Peripheral blood morphology
perioperative medicine
risk
monocytes
Opis:: Introduction. White blood cell (WBC) count constitutes a part of routine peripheral blood examination (FBC, full blood count). Precise analysis of leukocytes’ parameters in blood smear is usually performed only when leukopenia or leukocytosis is found. We aimed to assess the usefulness of leukocytes’ smear test in predicting in-hospital death of patients undergoing high-risk gastrointestinal (GI) surgery. Materials & Methods. We prospectively enrolled 101 subjects undergoing high-risk GI surgery from 01.01.2017 till 31.12.2017. Blood tests were performed preoperatively, with analysis focused on the assessment of total WBC count, as well as the count of neutrophils (NEUT), lymphocytes (LYM), monocytes (MONO), eosinophils (EOS) and basophils (BASO). Indices of NEUT/LYM (NLR) and MONO/LYM (MLR) were calculated. In-hospital mortality was considered the outcome. Results. Mortality reached 5%. There was no significant difference in WBC count between survivors and the deceased (p=0.2) and WBC failed to predict the outcome (AUC=0.69; p=0.3). MONO (p=0.009) and BASO (p=0.02) counts, as well as MLR (p=0.007) were significantly higher in patients who died. MONO count and MLR index predicted in-hospital death with good accuracy, respectively: AUC[MONO]=0.85 (p<0.001) and AUC[MLR]=0.86 (p<0.001). Other investigated parameters played no significant role in outcome prediction. Conclusion. Routine peripheral blood smear evaluation should be considered in all patients undergoing high-risk GI surgery, because the number of monocytes can be a valuable predictor of in-hospital death.
Źródło:: Polish Journal of Surgery; 2019, 91, 1; 22-28
0032-373X
2299-2847
Pojawia się w:: Polish Journal of Surgery
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Cellular immune response of pigeons in the conditions of endotoxin fever and pyrogenic tolerance
Autorzy:: Dudek, K.
Bednarek, D.
Powiązania:: https://bibliotekanauki.pl/articles/30231.pdf
Data publikacji:: 2011
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: cellular immune response
endotoxin fever
fever
pigeon
pyrogenic tolerance
bird
Escherichia coli
defense mechanism
bacteria
Salmonella
Pseudomonas
peripheral blood
immunophenotyping
Opis:: The aim of this study was to investigate changes in selected parameters of cellular immune response in the conditions of endotoxin fever and pyrogenic tolerance in pigeons. On the first day of observation the experimental birds (n=18) were intravenously injected with Escherichia coli LPS at a dose of 10 μg/kg b.w., while the control animals (n=6) received apyrogenic physiological saline also in the form of injection. On the second and the third day of the experiment LPS was injected additionally at 24 h intervals. Four and a half hours after the saline and pyrogen administration blood samples were collected from the control and experimental pigeons. The following immunological assays were performed: WBC, leucogram and immunophenotyping of lymphocyte subsets in peripheral blood, i.e. CD 3+ (T lymphocytes), CD 4+ (T helper lymphocytes) and CD 8+ (T suppressor/ cytotoxic lymphocytes) cells. In the conditions of endotoxin fever (i.e. after the first LPS injection) leucopenia, monocytopenia, heterophilia and eosinophilia were observed. Additionally, the immunophenotyping of peripheral blood lymphocytes indicated an increase in percentage of CD 3+, CD 4+ and CD 8+ cells in response to the single injection of LPS. In contrast, the consecutive injections of LPS, which created a pyrogenic tolerance effect, caused a decrease in WBC value, heteropenia, eosinopenia and lymphocytosis. Moreover, during this state an increase in percentage of CD 3+ and CD 8+ cells was demonstrated in contrast to the percentage of CD 4+ lymphocytes. The general tendencies in cellular immune response of the affected pigeons in the conditions of endotoxin fever and pyrogenic tolerance aim at activation of defence mechanisms against LPS for its prompt elimination from the animal’s organism.
Źródło:: Polish Journal of Veterinary Sciences; 2011, 14, 1
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: Contact allergy to nickel: Patch test score correlates with IL-5, but not with IFN-gamma nickel-specific secretion by peripheral blood lymphocytes
Autorzy:: Czarnobilska, E
Jenner, B.
Kaszuba-Zwoinska, J.
Kapusta, M.
Obtulowicz, K.
Thor, P.
Spiewak, R.
Powiązania:: https://bibliotekanauki.pl/articles/49735.pdf
Data publikacji:: 2009
Wydawca:: Instytut Medycyny Wsi
Tematy:: allergic contact dermatitis
patch test
contact allergy
lymphocyte
interleukin 5
interferon gamma
risk estimation
nickel
peripheral blood
chronic disease
Źródło:: Annals of Agricultural and Environmental Medicine; 2009, 16, 1; 37-41
1232-1966
Pojawia się w:: Annals of Agricultural and Environmental Medicine
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 6.

Tytuł:: Dose- and time-dependent micronucleus induction in peripheral erythrocytes of catfish, Heteropneustes fossilis (Bloch) by zinc
Autorzy:: Talapatra, S.N.
Banerjee, P.
Mukhopadhyay, A.
Powiązania:: https://bibliotekanauki.pl/articles/11433.pdf
Data publikacji:: 2014
Wydawca:: Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:: micronucleus
peripheral blood
erythrocyte
catfish
Heteropneustes fossilis
zinc
genotoxicity
Opis:: As far as the detection of metal genotoxicity in fish is concerned, micronucleus (MN) test is considered an extremely suitable measure. In this study, frequencies of micronucleated erythrocytes were scored in peripheral blood of catfish, Heteropneustes fossilis (bloch) after acute in-vivo exposure of zinc at different concentrations (5, 10 and 30 ppm) in the laboratory condition. These three concentrations of zinc were tested at different durations such as 24h, 48h, 72h and 96h respectively. Highly significant (P < 0.001) increased values were obtained for MN frequencies in the peripheral erythrocytes of exposed fishes compared to control groups of fishes. These results confirm that dose- and time-dependent micronucleation in the peripheral erythrocytes of fish after short-term exposure to zinc could provide valuable information regarding zinc containing effluent quality and also help in genetic biomonitoring with this test model. In this context safe concentration of zinc vis-a-vis genotoxicity range could be evaluated for future studies.
Źródło:: International Letters of Natural Sciences; 2014, 04
2300-9675
Pojawia się w:: International Letters of Natural Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 7.

Tytuł:: Effect of Biolex Beta-HP on phagocytic activity and oxidative metabolism of peripherial blood granulocytes and monocytes in rats intoxicated by cyclophosphamide
Autorzy:: Wojcik, R
Powiązania:: https://bibliotekanauki.pl/articles/32265.pdf
Data publikacji:: 2010
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: beta-1,3-1,6-D-glucan
cyclophosphamide
rat
phagocytic activity
oxidative metabolism
granulocyte
monocyte
flow cytometry
peripheral blood
immunosuppression
blood neutrophil
Opis:: The objective of this study was to determine the effect of ß-l,3/l,6-D-glucan (Biolex-Beta HP) on the phagocytic activity and oxidative metabolism of peripheral blood granulocytes and monocytes in rats intoxicated by cyclophosphamide. The experimental material comprised 40 adult Wistar rats aged 14 weeks, divided into two equal groups, a control group and an experimental group, each of 20 adult rats, including 10 males and 10 females. In the course of 3 successive days, 20 rats from the experimental group were administered cyclophosphamide intramuscularly at a dose of 50 mg/kg body weight/day. On the 8th day of the experiment, 10 control group (K) rats and 10 experimental group (C) rats were sacrificed. Arterial blood samples were collected and diluted with heparin to determine and compare the phagocytic activity (Phatogest) and oxidative metabolism (Bursttest) of peripheral blood granulocytes and monocytes by flow cytometry. Starting on the 8lh day of the experiment, the feed of the remaining 10 rats from the experimental group (C+G) and 10 rats from the control group (G) was supplemented with Biolex-Beta HP at a dose of 50 mg/kg body weight/day for 14 consecutive days. On day 22, arterial blood samples were collected from all (C+G) and (C) group rats, diluted with heparin to determine and compare the phagocytic activity and oxidative metabolism of peripheral blood granulocytes and monocytes by flow cytometry. The results showed that Biolex-Beta HP modulated the phagocytic activity and oxidative metabolism of blood neutrophils and monocytes suppressed by cyclophosphamide in rats. The immunorestoring activity of Biolex-Beta HP was observed in this study.
Źródło:: Polish Journal of Veterinary Sciences; 2010, 13, 1; 181-188
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 8.

Tytuł:: Effect of suberoylanilide hydroxamic acid on peripheral blood mononuclear cell cytotoxicity towards tumor cells in canines
Autorzy:: Oyamada, T.
Okano, S.
Powiązania:: https://bibliotekanauki.pl/articles/2087212.pdf
Data publikacji:: 2021
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: antitumor effect
canines
cytotoxicity
NKG2D receptor
peripheral blood mononuclear cell
suberoylanilide hydroxamic acid
Źródło:: Polish Journal of Veterinary Sciences; 2021, 24, 1; 35-41
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 9.

Tytuł:: Evaluation of DNA damage in white blood cells of healthy human volunteers using the alkaline comet assay and the chromosome aberration test
Autorzy:: Kopjar, Nevenka
Želježić, Davor
Garaj-Vrhovac, Verica
Powiązania:: https://bibliotekanauki.pl/articles/1041246.pdf
Data publikacji:: 2006
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: peripheral blood
chromosome aberration test
white blood cells
DNA damage
alkaline comet assay
lymphocytes
Opis:: The present study was undertaken to contribute to the characterization of the degree of variability in baseline damage in white blood cells from control population, and to investigate how this variability is associated with external and internal factors. Altogether 170 healthy volunteers, randomly selected from the general population of the Republic of Croatia, participated in the study. Two sensitive tests: the alkaline comet assay and the chromosome aberration test were applied to study the background levels of DNA damage in their white blood cells. The results point to inter-individual differences, indicating different genome sensitivity. As revealed by both assays, the background levels of DNA damage were mostly influenced by smoking habit as well as medical exposure (especially to diagnostic X-rays). Sex and age of subjects did not significantly influence the values of DNA damage recorded in the white blood cells. Although higher levels of DNA damage were recorded in blood samples collected during winter and autumn, they were mostly influenced by medicinal exposure and smoking habit. Statistical evaluation of the data confirmed that a positive correlation exists between DNA migration and the number of long-tailed nuclei found with the comet assay and the total number of chromosome aberrations. The data obtained can serve as control values in forthcoming biomonitoring studies.
Źródło:: Acta Biochimica Polonica; 2006, 53, 2; 321-336
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 10.

Tytuł:: Evaluation of the influence of meloxicam and flunixin meglumine on the apoptosis of peripheral blood CD4plus and CD8plus T cells in calves
Autorzy:: Maslanka, T
Jaroszewski, J.J.
Markiewicz, W.
Jabubowski, P.
Powiązania:: https://bibliotekanauki.pl/articles/31556.pdf
Data publikacji:: 2010
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: peripheral blood
T cell
calf
CD4plus T cell
CD8plus T cell
apoptosis
meloxicam
flunixin meglumine
lymphocyte
antiinflammatory drug
Opis:: The aim of the study was to determine whether treatment with recommended doses of meloxicam or flunixin had an effect on the apoptosis of peripheral blood T lymphocytes in calves. The study was carried out on 4-5 months old calves (n = 24, 8 per group). Experimental animals were injected subcutaneously with a single dose of 0.5 mg . kg-1 of meloxicam or intravenously with 3 doses of 2.2 mg . kg-1 day-1 of flunixin. The non-treatment animals served as control. Blood samples were taken at day 0 and at days 1, 2, 3, 5, 7 and 14 after the first NSAIDs injection. Apoptosis was determined by flow cytometry using Annexin V-PE/7-AAD staining. The kinetic analysis of apoptosis in the total lymphocyte population, as well as in the CD4+ and CD8+ subsets did not reveal significant differences in the frequency of early apoptotic cells between control and experimental groups throughout the period studied. Although, 24 h after administration of the first dose of NSAIDs, late-stage apoptosis/necrosis was significantly increased in the total lymphocyte population (the meloxicam group), as well as in the CD4+ (the meloxicam group and the flunixin group) and CD8+ (the flunixin group) subsets of T cells. However, this disturbance was transient, relatively poorly expressed and, thus, unlikely to be of clinical significance. Our results indicate that the use of meloxicam or flunixin in accordance with the recommended dosage regimen in cattle do not have a clinically significant influence on apoptosis of peripheral blood T cells.
Źródło:: Polish Journal of Veterinary Sciences; 2010, 13, 1; 3-12
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 11.

Tytuł:: Functional studies on sciatic nerve blood flow in respect to its vascular supply and tonic neural activity
Autorzy:: Stolarczyk, A.
Papierski, K.
Adamczyk, G.
Sinski, M.
Sawionek, L.
Przybylski, J.
Powiązania:: https://bibliotekanauki.pl/articles/69564.pdf
Data publikacji:: 2000
Wydawca:: Polskie Towarzystwo Fizjologiczne
Tematy:: blood vessel
nerve fibre
blood flow
peripheral nerve
sciatic nerve
rat
Źródło:: Journal of Physiology and Pharmacology; 2000, 51, 3
0867-5910
Pojawia się w:: Journal of Physiology and Pharmacology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 12.

Tytuł:: Human cytomegalovirus infection as a lifelong health problem
Autorzy:: Figlerowicz, M.
Modlinska-Cwalinska, A.
Mania, A.
Mazur-Melewska, K.
Kemnitz, P.
Jonczyk-Potoczna, K.
Sluzewski, W.
Powiązania:: https://bibliotekanauki.pl/articles/80892.pdf
Data publikacji:: 2011
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: human cytomegalovirus
cytomegalovirus
infection
health problem
patient
immune system
peripheral blood cell
atherosclerosis
cardiovascular disease
myocardial ischaemia
myocardial infarction
hypertension
neoplastic transformation
child
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2011, 92, 4
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 13.

Tytuł:: INCREASED LEVELS OF INTERLEUKIN-10 EXPRESSION COMPARED TO INTERLEUKIN-6 IN LEUKOCYTES OF HEALTHY SUBJECTS. COULD IT BE USEFUL IN THE FUTURE OF THE DEPRESSION DIAGNOSIS?
Autorzy:: Grzybkowska, Agata
Anczykowska, Katarzyna
Pyczek, Joanna M
Żychowska, Małgorzata
Powiązania:: https://bibliotekanauki.pl/articles/2137747.pdf
Data publikacji:: 2018-09-17
Wydawca:: Fundacja Edukacji Medycznej, Promocji Zdrowia, Sztuki i Kultury Ars Medica
Tematy:: depressive disorder
gene expression
inflammation
physical activity
peripheral blood mononuclear cells
Opis:: It is a well known, that the physical activity promotes mental health. Physically active people relatively rarely suffer from psychosomatic and depressive disorders. It is possible that the differences in gene activity in peripheral blood leukocytes may be associated with depression, especially genes participating in inflammatory response. Thus, the aim of our study was to investigate the levels of IL6 and IL10 mRNA and IL10/IL6 ratio in peripheral blood leukocytes in healthy, physically active individuals. One hundred healthy young men (20-23 years old) participated in this study. All of them declared regular physical activity. Participants were non-smokers, and consumed alcohol occasionally. To access genes expression, 2 ml of venous blood was collected. RNA isolation was performed and then the relative expression of IL6 and IL10 was calculated using quantitative real-time polymerase chain reaction (Q-RT-PCR). Pro and anti-inflammatory balance was calculated as 2^relative of IL10/ 2^relative expression of IL6. Low expression of tested genes was found in healthy young men. Mean expression for IL6 was 2^0.051 (n=90) and for IL10 2^0.08 (n=98). Mean ratio IL10/IL6 was 2^1.58. Higher expression of IL10 compared to IL6 may be essential not only for the physical performance but also for the mental health. Diverse reports in the literature may be associated with choosing various control groups, i.e. sedentary or older individuals. It is possible, that measuring the expression of IL6 and IL10 (especially the ratio IL10/IL6) in peripheral blood leukocytes may be useful in the assessment of depressive disorders. Thus, molecular study of active young men can confirm the need for physical activity among people suffering from depression, but further studies are needed, particularly among people with psychosomatic disorders.
Źródło:: Acta Neuropsychologica; 2018, 16(3); 243-248
1730-7503
2084-4298
Pojawia się w:: Acta Neuropsychologica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 14.

Tytuł:: Indukcja mikrojąder w krwi obwodowej i szpiku kostnym samców myszy narażanych subchronicznie na działanie promieniowania X i bisfenolu A
Induction of micronuclei in peripheral blood and bone marrow reticulocytes of male mice after subchronic exposure to X-rays and bisphenol A
Autorzy:: Radzikowska, J.
Gajowik, A.
Dobrzynska, M.
Powiązania:: https://bibliotekanauki.pl/articles/873526.pdf
Data publikacji:: 2012
Wydawca:: Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny
Tematy:: bisfenol A
promieniowanie jonizujace
dzialanie skojarzone
mikrojadra
krew obwodowa
szpik kostny
retikulocyty
myszy
samce
zwierzeta laboratoryjne
bisphenol A
ionizing radiation
combined action
micronucleus
peripheral blood
bone marrow
reticulocyte
mouse
male
laboratory animal
Opis:: Wprowadzenie.Promieniowanie jonizujące i ksenoestrogeny występują powszechnie w środowisku człowieka. Bisfenol A (BPA) używany jest podczas produkcji poliwęglanów oraz żywic epoksydowych, stanowiących składnik m.in. soczewek do okularów, wypełnień dentystycznych, płyt CD, szyb okiennych, pokryw instrumentów, opakowań oraz pojemników na napoje, ale także wyrobów dla dzieci, butelek, talerzyków, kubeczków oraz elementów smoczków. Żywice epoksydowe wchodzą też w skład powłok wewnętrznych pojemników do przechowywania żywności. Promieniowanie jonizujące wykorzystywane jest m.in. w diagnostyce rentgenowskiej, terapii chorób nowotworowych, w przemyśle, nauce. Cel badań.Celem badań było określenie wpływu bisfenolu A, promieniowania X oraz skojarzonego działania obu czynników na indukcję mikrojąder w retikulocytach krwi obwodowej i szpiku kostnego myszy laboratoryjnych. Materiał i metoda.Doświadczenie prowadzono na samcach myszy Pzh: Sfis przez 8 tygodni. Zwierzętom podawano bisfenol A w wodzie do picia (5 mg/kg mc, 10 mg/kg mc, 20 mg/kg mc), napromieniano dawką 0,05 Gy promieniowania X albo poddawano skojarzonemu działaniu obu tych czynników (0,05 Gy + 5 mg/kg mc BPA). Krew z żyły ogonowej pobierano po upływie 1, 4 i 8 tygodni od rozpoczęcia ekspozycji, a szpik kostny tylko po zakończeniu narażania. Oceniano częstość występowania mikrojąder w retikulocytach. Wyniki. Zarówno bisfenol A jak i promieniowanie jonizujące stymulowały indukcję mikrojąder w retikulocytach krwi obwodowej i szpiku kostnego. W następstwie napromieniania zwierząt promieniowaniem X indukcja mikrojąder wzrastała, podczas gdy w rezultacie podawania bisfenolu A malała proporcjonalnie do czasu trwania ekspozycji.. Skojarzone działanie promieniowania jonizującego i BPA indukowało występowanie mikrojąder ze znacznie wyższą częstością w porównaniu do efektów działania samego BPA. Częstość występowania mikrojąder w krwi obwodowej zwiększała się w miarę upływu czasu od rozpoczęcia doświadczenia. W szpiku kostnym we wszystkich grupach obserwowano znacznie niższą liczebność retikulocytów z mikrojądrami niż w krwi obwodowej. Wnioski. Subchroniczne narażenie na bisfenol A prowadzi do zmniejszenia wrażliwości materiału genetycznego retikulocytów na indukcję uszkodzeń. Promieniowanie X jest prawdopodobnie czynnikiem decydującym o uszkodzeniu DNA w następstwie skojarzonego działania.
Background. Ionizing radiation and xenoestrogens are widely present in the human environment. Bisphenol A (BPA) is used to manufacture polycarbonate plastics, epoxy and polyester resins. BPA is present in a great variety of products including: baby bottles, compact disks, thermal paper, safety helmets, bullet resistant laminate, plastic windows, car parts, adhesives, protective coatings, powder paints, polycarbonate bottles and containers, the sheathing of electrical and electronic parts, dental fillings. Food and beverage cans are protected from rusting and corrosion by the application of epoxy resins as inner coatings. Human activities involving the use of radiation and radioactive materials in industry, agriculture and research cause radiation exposure in addition to natural exposure coming from cosmic rays and naturally occurring radioactive substances. Objective. The aim of the study was to estimate the effects of bisphenol A, X-rays and combined exposure to X-rays and bisphenol A on the induction of micronuclei in the peripheral blood and in bone marrow reticulocytes of laboratory mice. Material and method. Pzh-Sfis male mice were exposed for 8 weeks. Animals were treated with bisphenol A diluted in drinking water (5 mg/kg bw, 10 mg/kg bw, 20 mg/kg bw), irradiated 0.05 Gy of X-rays or exposed to a combination of both (0.05 Gy + 5 mg/kg bw BPA). The samples of peripheral blood were taken at 1, 4 and 8 week following the start of exposure, whereas the bone marrow after the end of experiment, only. The induction of micronuclei in reticulocytes were evaluated by using fluorescence microscope. Results. Bisphenol A as well as ionizing radiation stimulated induction of micronuclei in peripheral blood and bone marrow reticulocytes. After the irradiation the level of micronuclei increased, whereas after exposure to BPA decreased related to time expired from beginning of experiment. Combined exposure of ionizing radiation and bisphenol A induced significantly higher frequency of micronuclei compared to the effect produced by BPA alone. The frequency of micronuclei in peripheral blood reticulocytes increased during the experiment. In all groups, the significantly lower induction of micronuclei in reticulocytes of bone marrow than of peripheral blood were observed. The levels of micronuclei in mice exposed to a combination of X-rays and BPA or to irradiation alone were slightly higher compared to those administered to BPA alone. Conclusions. Bisphenol A induced micronuclei in peripheral blood and bone marrow reticulocytes. Subchronic BPA exposure leads to diminished sensitivity of genetic material of reticulocytes on the induction of damage. X-rays is probably the agent which decided about DNA damage following combined exposure.
Źródło:: Roczniki Państwowego Zakładu Higieny; 2012, 63, 1
0035-7715
Pojawia się w:: Roczniki Państwowego Zakładu Higieny
Dostawca treści:: Biblioteka Nauki