Temat: peripheral blood - Katalog OPAC zbiorów

Tytuł:: Ocena czestosci wystepowania mikrojader w erytrocytach myszy eksponowanych subchronicznie na promieniowanie jonizujace i nonylofenol
Frequency of micronuclei in erythrocytes of mice subchronic exposed to ionising radiation and nonylphenol
Autorzy:: Dobrzynska, M M
Powiązania:: https://bibliotekanauki.pl/articles/871848.pdf
Data publikacji:: 2008
Wydawca:: Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny
Tematy:: zwierzeta laboratoryjne
myszy
promieniowanie jonizujace
nonylofenol
krew obwodowa
szpik kostny
mikrojadra
retikulocyty
erytrocyty polichromatyczne
laboratory animal
mouse
ionizing radiation
nonylphenol
peripheral blood
bone marrow
micronucleus
reticulocyte
polychromatic erythrocyte
Opis:: Celem pracy było określenie indukcji mikrojąder w retikulocytach i erytrocytach polichromatycznych myszy pod wpływem subchronicznej ekspozycji na promieniowanie X, nonylofenol (NF) lub na skojarzone działanie obu czynników. Myszy Pzh: SFIS narażane były przez 8 tygodni, 5 razy w tygodniu. Dawki wynosiły 0.05 Gy lub 0,10 Gy promieniowania X, 25 mg/kg mc lub 50 mg/kg mc nonylofenolu, a w przypadku skojarzonego działania 0,05 Gy + 25 mg/kg mc NF lub 0,10 Gy + 50 mg/kg mc NF. Zarówno promieniowania X, jak i NF działając oddzielnie indukowały powstawanie mikrojąder w retikulocytach i erytrocytach polichromatycznych myszy. Skojarzone działanie obu czynników w retikulocytach krwi obwodowej oraz w erytrocytach polichromatycznych szpiku kostnego indukowało zwiększoną częstość występowania mikrojąder w porównaniu do efektów działania każdego z czynników oddzielnie. W retikulocytach szpiku kostnego skojarzone działanie obu czynników w małych dawkach (0,05 Gy + 50 mg/kg mc NF) powodowało zwiększenie efektu mutagennego. Natomiast, w przypadku skojarzenia obu czynników w większych dawkach (0,10 Gy + 50 mg/kg mc NF), nonylofenol może wywierać efekt ochronny w stosunku do DNA retikulocytów powodując zmniejszenie uszkodzeń indukowanych przez promieniowanie X.
The aim of the research was to investigate the level of micronuclei induction in reticulocytes and polychromatic erythrocytes of mice following subchronic exposure to X-rays, nonylphenol (NP) or to a combination of both. Male mice Pzh:SFIS were exposed during 8 weeks, 5 day per week to doses 0.05 Gy and 0.10 Gy of X-rays, 25 mg/kg mc and 50 mg/kg bw of nonylphenol as well as to 0.05 Gy + 25 mg/kg bw NP and 0.10 Gy + 50 mg/kg bw NP for combined exposure. Both X-rays and NP, acting alone induced micronuclei in reticulocytes and in polychromatic erythrocytes of mice. Combined X-rays-NP exposure of peripheral blood reticulocytes and bone marrow polychromatic erythrocytes caused enhanced frequency of micronuclei compared to the effect of each agent alone. In bone marrow reticulocytes, combined exposure in lower doses (0.05 Gy + 25 mg/kg bw NP) induced enhanced mutagenic effect. Contrary, after combined exposure to both agents in higher doses (0.10 Gy + 50 mg/kg bw NP), nonylphenol may protected DNA of reticulocytes against damage induced by X-rays.
Źródło:: Roczniki Państwowego Zakładu Higieny; 2008, 59, 3; 309-318
0035-7715
Pojawia się w:: Roczniki Państwowego Zakładu Higieny
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 6.

Tytuł:: Cellular immune response of pigeons in the conditions of endotoxin fever and pyrogenic tolerance
Autorzy:: Dudek, K.
Bednarek, D.
Powiązania:: https://bibliotekanauki.pl/articles/30231.pdf
Data publikacji:: 2011
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: cellular immune response
endotoxin fever
fever
pigeon
pyrogenic tolerance
bird
Escherichia coli
defense mechanism
bacteria
Salmonella
Pseudomonas
peripheral blood
immunophenotyping
Opis:: The aim of this study was to investigate changes in selected parameters of cellular immune response in the conditions of endotoxin fever and pyrogenic tolerance in pigeons. On the first day of observation the experimental birds (n=18) were intravenously injected with Escherichia coli LPS at a dose of 10 μg/kg b.w., while the control animals (n=6) received apyrogenic physiological saline also in the form of injection. On the second and the third day of the experiment LPS was injected additionally at 24 h intervals. Four and a half hours after the saline and pyrogen administration blood samples were collected from the control and experimental pigeons. The following immunological assays were performed: WBC, leucogram and immunophenotyping of lymphocyte subsets in peripheral blood, i.e. CD 3+ (T lymphocytes), CD 4+ (T helper lymphocytes) and CD 8+ (T suppressor/ cytotoxic lymphocytes) cells. In the conditions of endotoxin fever (i.e. after the first LPS injection) leucopenia, monocytopenia, heterophilia and eosinophilia were observed. Additionally, the immunophenotyping of peripheral blood lymphocytes indicated an increase in percentage of CD 3+, CD 4+ and CD 8+ cells in response to the single injection of LPS. In contrast, the consecutive injections of LPS, which created a pyrogenic tolerance effect, caused a decrease in WBC value, heteropenia, eosinopenia and lymphocytosis. Moreover, during this state an increase in percentage of CD 3+ and CD 8+ cells was demonstrated in contrast to the percentage of CD 4+ lymphocytes. The general tendencies in cellular immune response of the affected pigeons in the conditions of endotoxin fever and pyrogenic tolerance aim at activation of defence mechanisms against LPS for its prompt elimination from the animal’s organism.
Źródło:: Polish Journal of Veterinary Sciences; 2011, 14, 1
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 7.

Tytuł:: Very High Concentration of D-Dimers in Portal Blood in Patients with Pancreatic Cancer
Autorzy:: Durczyński, Adam
Szymański, Dariusz
Nowicki, Michał
Hogendorf, Piotr
Poznańska, Grażyna
Strzelczyk, Janusz
Powiązania:: https://bibliotekanauki.pl/articles/1396873.pdf
Data publikacji:: 2012-12-01
Wydawca:: Index Copernicus International
Tematy:: D-dimers
pancreatic cancer
portal blood
peripheral blood
Opis:: Nowadays, increasing attention has been focused on relation between increased D-dimer levels and cancer among patients without detectable thrombosis. The aim of the study was to measure plasma D-dimer levels in portal and peripheral blood in pancreatic cancer patients with absence of venous thromboembolism. Material and methods. Fifteen consecutive patients hospitalized in the Department of General and Transplant Surgery of Medical University in Łódź, from January to March 2012 who underwent surgery due to a pancreatic cancer were enrolled. At laparotomy, portal and peripheral blood were sampled concurrently. D-dimer and fibrinogen levels were measured. Moreover, to investigate overall coagulation function prothrombin time (PT), prothrombin index (PI), international normalized ratio (INR), thrombin time (TT), activated partial thromboplastin time (APTT), TT and APTT index were evaluated. Results. Peripheral plasma D-dimmer levels above normal range were found in 10/15 patients (66,67%), whereas D-dimer above normal values were confirmed in all portal blood samples. Mean D-dimer values were higher in portal than in peripheral blood (3279.37 vs 824.64, by 297%, p=0,025). These discrepancies were accompanied by normal limits of portal and peripheral levels of fibrinogen and comparable coagulation function indexes. Conclusion. Our preliminary study showed the close relation between activation of hemostasis, reflected by elevated D-dimers in portal blood and presence of pancreatic cancer. These data suggest that measurement of portal blood D-dimer levels may be a potentially useful technique for screening the pancreatic cancer.
Źródło:: Polish Journal of Surgery; 2012, 84, 10; 521-525
0032-373X
2299-2847
Pojawia się w:: Polish Journal of Surgery
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 8.

Tytuł:: Human cytomegalovirus infection as a lifelong health problem
Autorzy:: Figlerowicz, M.
Modlinska-Cwalinska, A.
Mania, A.
Mazur-Melewska, K.
Kemnitz, P.
Jonczyk-Potoczna, K.
Sluzewski, W.
Powiązania:: https://bibliotekanauki.pl/articles/80892.pdf
Data publikacji:: 2011
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: human cytomegalovirus
cytomegalovirus
infection
health problem
patient
immune system
peripheral blood cell
atherosclerosis
cardiovascular disease
myocardial ischaemia
myocardial infarction
hypertension
neoplastic transformation
child
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2011, 92, 4
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 9.

Tytuł:: Reduced expression of AURKA in peripheral blood of breast cancer patients
Autorzy:: Goh, L.P.W.
See, E.U.H.
Chua, K.H.
Lee, P.-C.
Powiązania:: https://bibliotekanauki.pl/articles/81279.pdf
Data publikacji:: 2018
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: breast cancer
patient
Aurora kinase
gene expression
peripheral blood
cell cycle
quantitative real-time polymerase chain reaction
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2018, 99, 1
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 10.

Tytuł:: Model of binary biological mixture fractionation in gravity field and in centrifugal-force field
Model procesu frakcjonowania binarnych mieszanin biologicznych w polu grawitacji i w polu sił odśrodkowych
Autorzy:: Górka, Andrzej
Powiązania:: https://bibliotekanauki.pl/articles/1857125.pdf
Data publikacji:: 2020
Wydawca:: Wojskowa Akademia Techniczna im. Jarosława Dąbrowskiego
Tematy:: biological mixture fractionation
blood component sedimentation
mixture fractionation process model
peripheral blood ESR testing
fractionation in gravity field
fractionation in centrifugal-force field
frakcjonowanie mieszanin biologicznych
sedymentacja składników krwi
model procesu frakcjonowania mieszanin
badanie OB krwi obwodowej
frakcjonowanie w polu grawitacji
frakcjonowanie w polu sił odśrodkowych
Opis:: The publication describes a simplified model of the process of fractionation of binary biological mixtures in a closed reference system. The description of the model uses a balance of masses and forces forcing microparticle movement in a base fluid matrix. The movement, consistent with the direction of the excitation forces, was limited to independent migration channels with diameters comparable to the diameters of the migrating microparticles. In this model, the parameters of the densification process of the separated fraction in a single migration channel were applied to the entire volume of the fractioned mixture. The developed model applies to theoretical bases of the erythrocyte sedimentation rate (ESR).
W publikacji opisano uproszczony model procesu frakcjonowania binarnych mieszanin biologicznych w zamkniętym układzie odniesienia. W opisie modelu wykorzystano bilans mas i sił wymuszających ruch mikrocząstek w osnowie płynu bazowego. Ruch ten, zgodny z kierunkiem działania sił wymuszających ograniczono do niezależnych kanałów migracji o średnicy porównywalnej do średnicy migrujących mikrocząstek. W modelu tym, parametry procesu zagęszczania wyodrębnianej frakcji w pojedynczym kanale migracji odniesiono do całej objętości frakcjonowanej mieszaniny. Opracowany model dotyczy teoretycznych podstaw pomiaru opadu Biernackiego (OB).
Źródło:: Biuletyn Wojskowej Akademii Technicznej; 2020, 69, 2; 15-34
1234-5865
Pojawia się w:: Biuletyn Wojskowej Akademii Technicznej
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 11.

Tytuł:: Model of the Fractionation Process of Three- and Four-Component Biological Mixtures in the Gravity Field
Model procesu frakcjonowania trój- i czteroskładnikowych mieszanin biologicznych w polu grawitacyjnym
Autorzy:: Górka, Andrzej
Powiązania:: https://bibliotekanauki.pl/articles/1857113.pdf
Data publikacji:: 2020
Wydawca:: Wojskowa Akademia Techniczna im. Jarosława Dąbrowskiego
Tematy:: frakcjonowanie mieszanin biologicznych
sedymentacja składników krwi
model procesu frakcjonowania mieszanin
badanie OB krwi obwodowej
frakcjonowanie w polu grawitacyjnym
fractionation of biological mixtures
sedimentation of blood components
mixture fractionation process model
peripheral blood OB test
fractionation in gravity field
Opis:: The publication describes a simplified method of evaluation of basic parameters of the fractionation process in the gravity field of three- and four-component biological mixtures having physical parameters similar to peripheral blood. The description of the method contains the balance of masses and forces imposing independent movement of microparticles of different shape in the base fluid matrix. It was also assumed that the biological mixture as an uncompressible quasi-homogeneous liquid can be described using the basic laws of hydrodynamics. The presented method of evaluation of basic parameters of the fractionation process, in relation to complex biological mixtures, together with calculation examples, is a prelude to the extended peripheral blood test within the sedimentation rate of erythrocytes OB (ESR).
W publikacji opisano uproszczoną metodę oceny podstawowych parametrów procesu frakcjonowania w polu grawitacyjnym trój- i czteroskładnikowych mieszanin biologicznych o parametrach fizycznych podobnych do krwi obwodowej. W opisie metody wykorzystano bilans mas i sił wymuszających niezależny ruch mikrocząstek o różnym kształcie w osnowie płynu bazowego. Założono ponadto, że mieszaninę biologiczną jako nieściśliwy quasi-jednorodny płyn można opisać, wykorzystując podstawowe prawa hydrodynamiki. Prezentowana metoda oceny podstawowych parametrów procesu frakcjonowania w odniesieniu do złożonych mieszanin biologicznych wraz z przykładami obliczeń stanowi wstęp do rozszerzonego badania krwi obwodowej w zakresie oceny szybkości sedymentacji erytrocytów OB (ESR).
Źródło:: Biuletyn Wojskowej Akademii Technicznej; 2020, 69, 3; 35-57
1234-5865
Pojawia się w:: Biuletyn Wojskowej Akademii Technicznej
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 12.

Tytuł:: INCREASED LEVELS OF INTERLEUKIN-10 EXPRESSION COMPARED TO INTERLEUKIN-6 IN LEUKOCYTES OF HEALTHY SUBJECTS. COULD IT BE USEFUL IN THE FUTURE OF THE DEPRESSION DIAGNOSIS?
Autorzy:: Grzybkowska, Agata
Anczykowska, Katarzyna
Pyczek, Joanna M
Żychowska, Małgorzata
Powiązania:: https://bibliotekanauki.pl/articles/2137747.pdf
Data publikacji:: 2018-09-17
Wydawca:: Fundacja Edukacji Medycznej, Promocji Zdrowia, Sztuki i Kultury Ars Medica
Tematy:: depressive disorder
gene expression
inflammation
physical activity
peripheral blood mononuclear cells
Opis:: It is a well known, that the physical activity promotes mental health. Physically active people relatively rarely suffer from psychosomatic and depressive disorders. It is possible that the differences in gene activity in peripheral blood leukocytes may be associated with depression, especially genes participating in inflammatory response. Thus, the aim of our study was to investigate the levels of IL6 and IL10 mRNA and IL10/IL6 ratio in peripheral blood leukocytes in healthy, physically active individuals. One hundred healthy young men (20-23 years old) participated in this study. All of them declared regular physical activity. Participants were non-smokers, and consumed alcohol occasionally. To access genes expression, 2 ml of venous blood was collected. RNA isolation was performed and then the relative expression of IL6 and IL10 was calculated using quantitative real-time polymerase chain reaction (Q-RT-PCR). Pro and anti-inflammatory balance was calculated as 2^relative of IL10/ 2^relative expression of IL6. Low expression of tested genes was found in healthy young men. Mean expression for IL6 was 2^0.051 (n=90) and for IL10 2^0.08 (n=98). Mean ratio IL10/IL6 was 2^1.58. Higher expression of IL10 compared to IL6 may be essential not only for the physical performance but also for the mental health. Diverse reports in the literature may be associated with choosing various control groups, i.e. sedentary or older individuals. It is possible, that measuring the expression of IL6 and IL10 (especially the ratio IL10/IL6) in peripheral blood leukocytes may be useful in the assessment of depressive disorders. Thus, molecular study of active young men can confirm the need for physical activity among people suffering from depression, but further studies are needed, particularly among people with psychosomatic disorders.
Źródło:: Acta Neuropsychologica; 2018, 16(3); 243-248
1730-7503
2084-4298
Pojawia się w:: Acta Neuropsychologica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 13.

Tytuł:: SILDENAFIL UPREGULATES TUMOR NECROSIS FACTOR α PRODUCTION IN PERIPHERAL BLOOD MONONUCLEAR CELLS OF HEALTHY MEN - PRELIMINARY REPORT
Autorzy:: Kaleta, Beata
Boguska, Agnieszka
Borysowski, Jan
Górski, Andrzej
Powiązania:: https://bibliotekanauki.pl/articles/895585.pdf
Data publikacji:: 2019-02-28
Wydawca:: Polskie Towarzystwo Farmaceutyczne
Tematy:: cytokines
tumor necrosis factor alpha
Sildenafil
peripheral blood mononuclear cells
Opis:: Sildenafil is a selective type 5 phosphodiesterase (PDE5) inhibitor, commonly used in the treatment of erectile dysfunction (ED) and pulmonary arterial hypertension (PAH). The results of recent studies suggest that this drug modulates function of the immune system. However, only few studies were performed in humans. Therefore, the aim of this study was to investigate the ability of sildenafil to regulate the production of pro-inflammatory cytokines including tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β and IL-6 in peripheral blood mononuclear cells (PBMCs) from healthy men. Phorbol myristate acetate (PMA) - stimulated and unstimulated PBMCs from 16 participants were cultured in the absence or presence of sildenafil (400 ng/ml and 4 µg/ml). TNF-α, IL-1β and IL-6 concentrations in culture supernatants were measured by enzyme-linked immunosorbent assay (ELISA). Cell viability was assessed by trypan blue staining. Sildenafil at a concentration of 400 ng/ml significantly increased TNF-α production in stimulated PBMCs (p<0.05) but had no effects on IL-1β and IL-6. The drug did not affect PBMCs viability. This is the first report describing such effects of sildenafil in humans.
Źródło:: Acta Poloniae Pharmaceutica - Drug Research; 2019, 76, 1; 123-128
0001-6837
2353-5288
Pojawia się w:: Acta Poloniae Pharmaceutica - Drug Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 14.

Tytuł:: Evaluation of DNA damage in white blood cells of healthy human volunteers using the alkaline comet assay and the chromosome aberration test
Autorzy:: Kopjar, Nevenka
Želježić, Davor
Garaj-Vrhovac, Verica
Powiązania:: https://bibliotekanauki.pl/articles/1041246.pdf
Data publikacji:: 2006
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: peripheral blood
chromosome aberration test
white blood cells
DNA damage
alkaline comet assay
lymphocytes
Opis:: The present study was undertaken to contribute to the characterization of the degree of variability in baseline damage in white blood cells from control population, and to investigate how this variability is associated with external and internal factors. Altogether 170 healthy volunteers, randomly selected from the general population of the Republic of Croatia, participated in the study. Two sensitive tests: the alkaline comet assay and the chromosome aberration test were applied to study the background levels of DNA damage in their white blood cells. The results point to inter-individual differences, indicating different genome sensitivity. As revealed by both assays, the background levels of DNA damage were mostly influenced by smoking habit as well as medical exposure (especially to diagnostic X-rays). Sex and age of subjects did not significantly influence the values of DNA damage recorded in the white blood cells. Although higher levels of DNA damage were recorded in blood samples collected during winter and autumn, they were mostly influenced by medicinal exposure and smoking habit. Statistical evaluation of the data confirmed that a positive correlation exists between DNA migration and the number of long-tailed nuclei found with the comet assay and the total number of chromosome aberrations. The data obtained can serve as control values in forthcoming biomonitoring studies.
Źródło:: Acta Biochimica Polonica; 2006, 53, 2; 321-336
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 15.

Tytuł:: Evaluation of the influence of meloxicam and flunixin meglumine on the apoptosis of peripheral blood CD4plus and CD8plus T cells in calves
Autorzy:: Maslanka, T
Jaroszewski, J.J.
Markiewicz, W.
Jabubowski, P.
Powiązania:: https://bibliotekanauki.pl/articles/31556.pdf
Data publikacji:: 2010
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: peripheral blood
T cell
calf
CD4plus T cell
CD8plus T cell
apoptosis
meloxicam
flunixin meglumine
lymphocyte
antiinflammatory drug
Opis:: The aim of the study was to determine whether treatment with recommended doses of meloxicam or flunixin had an effect on the apoptosis of peripheral blood T lymphocytes in calves. The study was carried out on 4-5 months old calves (n = 24, 8 per group). Experimental animals were injected subcutaneously with a single dose of 0.5 mg . kg-1 of meloxicam or intravenously with 3 doses of 2.2 mg . kg-1 day-1 of flunixin. The non-treatment animals served as control. Blood samples were taken at day 0 and at days 1, 2, 3, 5, 7 and 14 after the first NSAIDs injection. Apoptosis was determined by flow cytometry using Annexin V-PE/7-AAD staining. The kinetic analysis of apoptosis in the total lymphocyte population, as well as in the CD4+ and CD8+ subsets did not reveal significant differences in the frequency of early apoptotic cells between control and experimental groups throughout the period studied. Although, 24 h after administration of the first dose of NSAIDs, late-stage apoptosis/necrosis was significantly increased in the total lymphocyte population (the meloxicam group), as well as in the CD4+ (the meloxicam group and the flunixin group) and CD8+ (the flunixin group) subsets of T cells. However, this disturbance was transient, relatively poorly expressed and, thus, unlikely to be of clinical significance. Our results indicate that the use of meloxicam or flunixin in accordance with the recommended dosage regimen in cattle do not have a clinically significant influence on apoptosis of peripheral blood T cells.
Źródło:: Polish Journal of Veterinary Sciences; 2010, 13, 1; 3-12
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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