Temat: peripheral blood - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Very High Concentration of D-Dimers in Portal Blood in Patients with Pancreatic Cancer
Autorzy:: Durczyński, Adam
Szymański, Dariusz
Nowicki, Michał
Hogendorf, Piotr
Poznańska, Grażyna
Strzelczyk, Janusz
Powiązania:: https://bibliotekanauki.pl/articles/1396873.pdf
Data publikacji:: 2012-12-01
Wydawca:: Index Copernicus International
Tematy:: D-dimers
pancreatic cancer
portal blood
peripheral blood
Opis:: Nowadays, increasing attention has been focused on relation between increased D-dimer levels and cancer among patients without detectable thrombosis. The aim of the study was to measure plasma D-dimer levels in portal and peripheral blood in pancreatic cancer patients with absence of venous thromboembolism. Material and methods. Fifteen consecutive patients hospitalized in the Department of General and Transplant Surgery of Medical University in Łódź, from January to March 2012 who underwent surgery due to a pancreatic cancer were enrolled. At laparotomy, portal and peripheral blood were sampled concurrently. D-dimer and fibrinogen levels were measured. Moreover, to investigate overall coagulation function prothrombin time (PT), prothrombin index (PI), international normalized ratio (INR), thrombin time (TT), activated partial thromboplastin time (APTT), TT and APTT index were evaluated. Results. Peripheral plasma D-dimmer levels above normal range were found in 10/15 patients (66,67%), whereas D-dimer above normal values were confirmed in all portal blood samples. Mean D-dimer values were higher in portal than in peripheral blood (3279.37 vs 824.64, by 297%, p=0,025). These discrepancies were accompanied by normal limits of portal and peripheral levels of fibrinogen and comparable coagulation function indexes. Conclusion. Our preliminary study showed the close relation between activation of hemostasis, reflected by elevated D-dimers in portal blood and presence of pancreatic cancer. These data suggest that measurement of portal blood D-dimer levels may be a potentially useful technique for screening the pancreatic cancer.
Źródło:: Polish Journal of Surgery; 2012, 84, 10; 521-525
0032-373X
2299-2847
Pojawia się w:: Polish Journal of Surgery
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Can the leukocyte`s parameters in peripheral blood smear predict the risk of in-hospital death of patients undergoing high-risk gastrointestinal surgery?
Autorzy:: Pluta, Michał
Krzych, Łukasz
Powiązania:: https://bibliotekanauki.pl/articles/1392359.pdf
Data publikacji:: 2019
Wydawca:: Index Copernicus International
Tematy:: Peripheral blood morphology
perioperative medicine
risk
monocytes
Opis:: Introduction. White blood cell (WBC) count constitutes a part of routine peripheral blood examination (FBC, full blood count). Precise analysis of leukocytes’ parameters in blood smear is usually performed only when leukopenia or leukocytosis is found. We aimed to assess the usefulness of leukocytes’ smear test in predicting in-hospital death of patients undergoing high-risk gastrointestinal (GI) surgery. Materials & Methods. We prospectively enrolled 101 subjects undergoing high-risk GI surgery from 01.01.2017 till 31.12.2017. Blood tests were performed preoperatively, with analysis focused on the assessment of total WBC count, as well as the count of neutrophils (NEUT), lymphocytes (LYM), monocytes (MONO), eosinophils (EOS) and basophils (BASO). Indices of NEUT/LYM (NLR) and MONO/LYM (MLR) were calculated. In-hospital mortality was considered the outcome. Results. Mortality reached 5%. There was no significant difference in WBC count between survivors and the deceased (p=0.2) and WBC failed to predict the outcome (AUC=0.69; p=0.3). MONO (p=0.009) and BASO (p=0.02) counts, as well as MLR (p=0.007) were significantly higher in patients who died. MONO count and MLR index predicted in-hospital death with good accuracy, respectively: AUC[MONO]=0.85 (p<0.001) and AUC[MLR]=0.86 (p<0.001). Other investigated parameters played no significant role in outcome prediction. Conclusion. Routine peripheral blood smear evaluation should be considered in all patients undergoing high-risk GI surgery, because the number of monocytes can be a valuable predictor of in-hospital death.
Źródło:: Polish Journal of Surgery; 2019, 91, 1; 22-28
0032-373X
2299-2847
Pojawia się w:: Polish Journal of Surgery
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Spectral properties of phthalocyanines incorporated into resting and stimulated human peripheral blood cells.
Autorzy:: Waszkowiak, Aneta
Frąckowiak, Danuta
Wiktorowicz, Krzysztof
Miyake, Jun
Powiązania:: https://bibliotekanauki.pl/articles/1043724.pdf
Data publikacji:: 2002
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: phthalocyanines
fluorescence
photodynamic therapy
human peripheral blood cells
Opis:: Human peripheral blood cells stimulated by phytohemagglutinin (which serve as a model of cancerous cells) and resting cells were incubated in dimethyl sulfoxide solutions of various phthalocyanines. In order to diminish the influence of atmospheric oxygen the cells were embedded in a polymer (polyvinyl alcohol) film. Fluorescence spectra of the samples were measured over two regions of excitation wavelengths: at 405 nm (predominant absorption of the cell material) and in the regions of strong absorption of phthalocyanines (at about 605 nm and 337 nm). The intrinsic emission of cell material became changed as a result both of cells' stimulation and of incubation of cells in dye solution. In most cases the stimulated cells when stained by dye exhibited higher long wavelength fluorescence intensity than resting cells. This suggests higher efficiency of dye incorporation into cancerous cells than into healthy cells. The absorption spectra of samples were also measured. The spectra of various phthalocyanines in incubation solvent, in polymer and in the cells embedded in polymer, were compared. The comparison of properties of the cells stimulated for different time periods enabled to establish the conditions of stimulation creating a population of cells incorporating a large number of sensitizing molecules.
Źródło:: Acta Biochimica Polonica; 2002, 49, 3; 633-641
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Dose- and time-dependent micronucleus induction in peripheral erythrocytes of catfish, Heteropneustes fossilis (Bloch) by zinc
Autorzy:: Talapatra, S.N.
Banerjee, P.
Mukhopadhyay, A.
Powiązania:: https://bibliotekanauki.pl/articles/11433.pdf
Data publikacji:: 2014
Wydawca:: Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:: micronucleus
peripheral blood
erythrocyte
catfish
Heteropneustes fossilis
zinc
genotoxicity
Opis:: As far as the detection of metal genotoxicity in fish is concerned, micronucleus (MN) test is considered an extremely suitable measure. In this study, frequencies of micronucleated erythrocytes were scored in peripheral blood of catfish, Heteropneustes fossilis (bloch) after acute in-vivo exposure of zinc at different concentrations (5, 10 and 30 ppm) in the laboratory condition. These three concentrations of zinc were tested at different durations such as 24h, 48h, 72h and 96h respectively. Highly significant (P < 0.001) increased values were obtained for MN frequencies in the peripheral erythrocytes of exposed fishes compared to control groups of fishes. These results confirm that dose- and time-dependent micronucleation in the peripheral erythrocytes of fish after short-term exposure to zinc could provide valuable information regarding zinc containing effluent quality and also help in genetic biomonitoring with this test model. In this context safe concentration of zinc vis-a-vis genotoxicity range could be evaluated for future studies.
Źródło:: International Letters of Natural Sciences; 2014, 04
2300-9675
Pojawia się w:: International Letters of Natural Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: A study on influence of different signs of air-ions on sister-chromatid exchange frequency and chromosome aberration in human peripheral blood lymphocytes
Autorzy:: Wiszniewski, A
Kretowicz, T.
Powiązania:: https://bibliotekanauki.pl/articles/2043653.pdf
Data publikacji:: 1999
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: blood lymphocyte
sister-chromatid exchange
man
peripheral blood lymphocyte
lymphocyte
chromosome aberration
Źródło:: Journal of Applied Genetics; 1999, 40, 2; 129-134
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 6.

Tytuł:: Ocena ekspresji Foxp3 i RORgamma t w jednojądrzastych komórkach krwi obwodowej u chorych z rakiem krtani jako wskaźników zaawansowania zmian nowotworowych — badania wstępne
Expression of Foxp3 and RORgamma t in peripheral blood mononuclear cells in patients with laryngeal carcinoma as indicators of tumor stage – preliminary study
Autorzy:: Papież, Paweł
Bryś, Magdalena
Lewy-Trenda, Iwona
Stasikowska, Olga
Woś, Jan
Papierz, Wielisław
Starska, Katarzyna
Powiązania:: https://bibliotekanauki.pl/articles/23352191.pdf
Data publikacji:: 2011
Wydawca:: Index Copernicus International
Tematy:: laryngeal carcinoma
Foxp3
RORgamma t
peripheral blood mononuclear cells
Opis:: The degree of activation of cells involved in cellular immune re-sponse against tumor antigens (cytotoxic lymphocytes Te) as well as efficiency of the mechanisms which promote immunosuppression (Treg — regulatory cells CD4±CD25±Foxp3±) may determine the course of the neoplastic disease. The aim of this study was to assess the function of autologous peripheral blood mononuclear cells (PBMCs) involved in the immunological processes on the basis of expression of Foxp3 and RORgamma t molecules as well as analysis of the relationships with clinical and morphological features of the tumor (pT and pN stage, G feature, degree of invasiveness according to the TFG classification) in laryngeal carcinoma. Materia! and methods: The analysis included a group of 59 patients with verified squamous celt carcinoma of the larynx. In the pathologic evaluation pTNM classification criteria, depth of invasion and degree of histological dif-ferentiation were used. Expression levels of mRNA for Foxp3 and RORgamma t in peripheral blood mononuclear cells by quantitative analysis of the amplified product in real time (real-time RT2-PCR) were evaluated. The level of Foxp3 and RORgamma t protein expression by Western blot analysis was determined. Results: In squamous celt carcinomas of the larynx, with the highest tumor aggressiveness the significantly highest level of mRNA and protein expres-sion for Foxp3 molecule were observed. The severity of Foxp3 expression at both gene and protein level were positively linearly correlated with the degree of local extent of the tumor (pT3-4), depth of invasion (invasion of cartilage) and the degree of histological differentiation (low-differentiated tumors G3). In the study group of laryngeal cancers significantly lower level of RORgamma t expression in carcinomas with less invasive changes (pT1-2, high-differentiated tumors G1, carcinomas with microinvasion without evi-dence of invasion beyond the lamina propria) was also noted. Conclusions: The study results indicate the important role of immune celt activity as indicators of advancement of clinical and morphological changes in squamous celt carcinoma of the larynx.
Źródło:: Polish Journal of Otolaryngology; 2011, 65, 5; 109-116
0030-6657
2300-8423
Pojawia się w:: Polish Journal of Otolaryngology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 7.

Tytuł:: SILDENAFIL UPREGULATES TUMOR NECROSIS FACTOR α PRODUCTION IN PERIPHERAL BLOOD MONONUCLEAR CELLS OF HEALTHY MEN - PRELIMINARY REPORT
Autorzy:: Kaleta, Beata
Boguska, Agnieszka
Borysowski, Jan
Górski, Andrzej
Powiązania:: https://bibliotekanauki.pl/articles/895585.pdf
Data publikacji:: 2019-02-28
Wydawca:: Polskie Towarzystwo Farmaceutyczne
Tematy:: cytokines
tumor necrosis factor alpha
Sildenafil
peripheral blood mononuclear cells
Opis:: Sildenafil is a selective type 5 phosphodiesterase (PDE5) inhibitor, commonly used in the treatment of erectile dysfunction (ED) and pulmonary arterial hypertension (PAH). The results of recent studies suggest that this drug modulates function of the immune system. However, only few studies were performed in humans. Therefore, the aim of this study was to investigate the ability of sildenafil to regulate the production of pro-inflammatory cytokines including tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β and IL-6 in peripheral blood mononuclear cells (PBMCs) from healthy men. Phorbol myristate acetate (PMA) - stimulated and unstimulated PBMCs from 16 participants were cultured in the absence or presence of sildenafil (400 ng/ml and 4 µg/ml). TNF-α, IL-1β and IL-6 concentrations in culture supernatants were measured by enzyme-linked immunosorbent assay (ELISA). Cell viability was assessed by trypan blue staining. Sildenafil at a concentration of 400 ng/ml significantly increased TNF-α production in stimulated PBMCs (p<0.05) but had no effects on IL-1β and IL-6. The drug did not affect PBMCs viability. This is the first report describing such effects of sildenafil in humans.
Źródło:: Acta Poloniae Pharmaceutica - Drug Research; 2019, 76, 1; 123-128
0001-6837
2353-5288
Pojawia się w:: Acta Poloniae Pharmaceutica - Drug Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 8.

Tytuł:: Investigations of the changes of peripheral blood T lymphocytes subpopulations using the flow cytofluorimetry technique in serum of T.spiralis infected mice
Autorzy:: Boczon, K.
Wargin, B.
Kostrzewa, A.
Derda, M.
Wiktorowicz, K.
Powiązania:: https://bibliotekanauki.pl/articles/836212.pdf
Data publikacji:: 1998
Wydawca:: Polskie Towarzystwo Parazytologiczne
Tematy:: peripheral blood
mouse
mice
serum
T lymphocyte
trichinellosis
blood
larva
cytofluorimetry technique
Trichinella spiralis
Źródło:: Annals of Parasitology; 1998, 44, 3
0043-5163
Pojawia się w:: Annals of Parasitology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 9.

Tytuł:: INCREASED LEVELS OF INTERLEUKIN-10 EXPRESSION COMPARED TO INTERLEUKIN-6 IN LEUKOCYTES OF HEALTHY SUBJECTS. COULD IT BE USEFUL IN THE FUTURE OF THE DEPRESSION DIAGNOSIS?
Autorzy:: Grzybkowska, Agata
Anczykowska, Katarzyna
Pyczek, Joanna M
Żychowska, Małgorzata
Powiązania:: https://bibliotekanauki.pl/articles/2137747.pdf
Data publikacji:: 2018-09-17
Wydawca:: Fundacja Edukacji Medycznej, Promocji Zdrowia, Sztuki i Kultury Ars Medica
Tematy:: depressive disorder
gene expression
inflammation
physical activity
peripheral blood mononuclear cells
Opis:: It is a well known, that the physical activity promotes mental health. Physically active people relatively rarely suffer from psychosomatic and depressive disorders. It is possible that the differences in gene activity in peripheral blood leukocytes may be associated with depression, especially genes participating in inflammatory response. Thus, the aim of our study was to investigate the levels of IL6 and IL10 mRNA and IL10/IL6 ratio in peripheral blood leukocytes in healthy, physically active individuals. One hundred healthy young men (20-23 years old) participated in this study. All of them declared regular physical activity. Participants were non-smokers, and consumed alcohol occasionally. To access genes expression, 2 ml of venous blood was collected. RNA isolation was performed and then the relative expression of IL6 and IL10 was calculated using quantitative real-time polymerase chain reaction (Q-RT-PCR). Pro and anti-inflammatory balance was calculated as 2^relative of IL10/ 2^relative expression of IL6. Low expression of tested genes was found in healthy young men. Mean expression for IL6 was 2^0.051 (n=90) and for IL10 2^0.08 (n=98). Mean ratio IL10/IL6 was 2^1.58. Higher expression of IL10 compared to IL6 may be essential not only for the physical performance but also for the mental health. Diverse reports in the literature may be associated with choosing various control groups, i.e. sedentary or older individuals. It is possible, that measuring the expression of IL6 and IL10 (especially the ratio IL10/IL6) in peripheral blood leukocytes may be useful in the assessment of depressive disorders. Thus, molecular study of active young men can confirm the need for physical activity among people suffering from depression, but further studies are needed, particularly among people with psychosomatic disorders.
Źródło:: Acta Neuropsychologica; 2018, 16(3); 243-248
1730-7503
2084-4298
Pojawia się w:: Acta Neuropsychologica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 10.

Tytuł:: Evaluation of DNA damage in white blood cells of healthy human volunteers using the alkaline comet assay and the chromosome aberration test
Autorzy:: Kopjar, Nevenka
Želježić, Davor
Garaj-Vrhovac, Verica
Powiązania:: https://bibliotekanauki.pl/articles/1041246.pdf
Data publikacji:: 2006
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: peripheral blood
chromosome aberration test
white blood cells
DNA damage
alkaline comet assay
lymphocytes
Opis:: The present study was undertaken to contribute to the characterization of the degree of variability in baseline damage in white blood cells from control population, and to investigate how this variability is associated with external and internal factors. Altogether 170 healthy volunteers, randomly selected from the general population of the Republic of Croatia, participated in the study. Two sensitive tests: the alkaline comet assay and the chromosome aberration test were applied to study the background levels of DNA damage in their white blood cells. The results point to inter-individual differences, indicating different genome sensitivity. As revealed by both assays, the background levels of DNA damage were mostly influenced by smoking habit as well as medical exposure (especially to diagnostic X-rays). Sex and age of subjects did not significantly influence the values of DNA damage recorded in the white blood cells. Although higher levels of DNA damage were recorded in blood samples collected during winter and autumn, they were mostly influenced by medicinal exposure and smoking habit. Statistical evaluation of the data confirmed that a positive correlation exists between DNA migration and the number of long-tailed nuclei found with the comet assay and the total number of chromosome aberrations. The data obtained can serve as control values in forthcoming biomonitoring studies.
Źródło:: Acta Biochimica Polonica; 2006, 53, 2; 321-336
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 11.

Tytuł:: The influence of glucocorticoid [depemedrol] treatment on the changes of peripheral blood T lymphocytes in serum of T.spiralis infected mice
Autorzy:: Boczon, K.
Kostrzewa, A.
Wargin, B.
Derda, M.
Wiktorowicz, K.
Powiązania:: https://bibliotekanauki.pl/articles/840872.pdf
Data publikacji:: 1998
Wydawca:: Polskie Towarzystwo Parazytologiczne
Tematy:: glucocorticoid
parasite
peripheral blood
infection
mouse
mice
serum
T lymphocyte
trichinellosis
depomedrol
blood
Trichinella spiralis
Źródło:: Annals of Parasitology; 1998, 44, 3
0043-5163
Pojawia się w:: Annals of Parasitology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 12.

Tytuł:: The applicability of the Migratest® kit for evaluating the chemotactic activity of peripheral blood neutrophils in goats on the example of animals supplemented with β-hydroxy-β-methylbutyrate (HMB)
Autorzy:: Wójcik, R.
Małaczewska, J.
Kaczorek-Łukowska, E.
Powiązania:: https://bibliotekanauki.pl/articles/2087425.pdf
Data publikacji:: 2019
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: goats
Migratest® kit
chemotactic activity of peripheral blood neutrophils
β-hydroxy-β-methylbutyrate
Źródło:: Polish Journal of Veterinary Sciences; 2019, 4; 725-733
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 13.

Tytuł:: Effect of suberoylanilide hydroxamic acid on peripheral blood mononuclear cell cytotoxicity towards tumor cells in canines
Autorzy:: Oyamada, T.
Okano, S.
Powiązania:: https://bibliotekanauki.pl/articles/2087212.pdf
Data publikacji:: 2021
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: antitumor effect
canines
cytotoxicity
NKG2D receptor
peripheral blood mononuclear cell
suberoylanilide hydroxamic acid
Źródło:: Polish Journal of Veterinary Sciences; 2021, 24, 1; 35-41
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 14.

Tytuł:: Reduced expression of AURKA in peripheral blood of breast cancer patients
Autorzy:: Goh, L.P.W.
See, E.U.H.
Chua, K.H.
Lee, P.-C.
Powiązania:: https://bibliotekanauki.pl/articles/81279.pdf
Data publikacji:: 2018
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: breast cancer
patient
Aurora kinase
gene expression
peripheral blood
cell cycle
quantitative real-time polymerase chain reaction
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2018, 99, 1
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki