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Wyświetlanie 1-15 z 87
Tytuł:
Dirofilarioza podskórna u psów
Subcutaneous dirofilariasis in dogs
Autorzy:
Sapierzynski, R.
Wojtczak, M.
Powiązania:
https://bibliotekanauki.pl/articles/861410.pdf
Data publikacji:
2014
Wydawca:
Krajowa Izba Lekarsko-Weterynaryjna
Tematy:
parazytologia weterynaryjna
psy
choroby zwierzat
choroby inwazyjne
dirofilarioza podskorna
pasozyty zwierzat
Dirofilaria repens
wystepowanie
chorobotworczosc
objawy chorobowe
diagnostyka
leczenie
profilaktyka
Źródło:
Życie Weterynaryjne; 2014, 89, 06
0137-6810
Pojawia się w:
Życie Weterynaryjne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ocena zaleznosci zarazenia pasozytami wewnetrznymi psow i kotow od przygotowania hodowlano-weterynaryjnego wlascicieli
The incidence of internal parasites in dogs and cats as dependent on the level of awareness among owners
Autorzy:
Michalczyk, M
Sokol, R.
Powiązania:
https://bibliotekanauki.pl/articles/839057.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
pasozyty wewnetrzne
psy
weterynaria
wiedza specjalistyczna
koty
czestotliwosc wystepowania
hodowla zwierzat
wlasciciele
nicienie
inwazja pasozytnicza
parazytologia weterynaryjna
Źródło:
Annals of Parasitology; 2008, 54, 3; 245-247
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ocena zależności zarażenia pasożytami wewnętrznymi psów i kotów od przygotowania hodowlano-weterynaryjnego wlaścicieli
The incidence of internal parasites in dogs and cats as dependent on the level of awareness among owners
Autorzy:
Michalczyk, M.
Sokół, R.
Powiązania:
https://bibliotekanauki.pl/articles/2143819.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
pasozyty wewnetrzne
psy
weterynaria
wiedza specjalistyczna
koty
czestotliwosc wystepowania
hodowla zwierzat
wlasciciele
nicienie
inwazja pasozytnicza
parazytologia weterynaryjna
Opis:
The objective of the present study was to determine the incidence of internal parasites in dogs and cats owned by the employees of the Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, and by students and their families, as well as in pets presented to veterinary clinics and in animals from shelters. The study, conducted from January to May 2008, involved 135 dogs and 35 cats. Feces samples were analyzed for parasites by flotation technique with the use of Darling’s solution, as described by Fülleborn. The highest percentage of parasite eggs was reported in feces samples collected from shelter dogs, including Toxocara and Toxascaris (9.1%), Uncinaria−Ancylostoma (12.1%) and Trichuris (12.1%), while in shelter cats the extensiveness of parasitic invasion was as follows: Toxocara cati – 61.5%, Toxascaris – 15.4%, Uncinaria−Ancylostoma – 7.7%, Isospora spp. – 7.7%. The lowest parasite incidence was observed among dogs and cats owned by veterinary students and members of their families. Of 20 examined animals, only one dog carried the eggs of Toxocara canis and one dog carried the eggs of Trichuris in their feces. Similar results were obtained in the group of pets owned by the employees of the Faculty of Veterinary Medicine: among 6 dogs and 13 cats examined in the study, Toxocara eggs were detected in one cat only (16.6%). In the group of pets presented to veterinary clinics, comprising 77 dogs and 8 cats, feces samples taken from 11 dogs contained the eggs of Toxocara canis, Toxascaris leonina, Uncinaria−Ancylostoma and Trichuris, while Isospora spp. oocysts were found in the stool of two cats.
Źródło:
Wiadomości Parazytologiczne; 2008, 54, 3; 245-247
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Przydatność ELISA do diagnozowania włośnicy u świń i dzików
The usefulness of ELISA for diagnosis of trichinellosis in pigs and wild boars
Autorzy:
Bień, J.
Powiązania:
https://bibliotekanauki.pl/articles/2144342.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
wlosnica
choroby pasozytnicze
diagnostyka weterynaryjna
Polska
walidacja
pasozyty
Warszawa konferencja
trzoda chlewna
dziki
wykorzystanie
parazytologia
konferencje
Trichinella
choroby zwierzat
test ELISA
wykrywanie
Opis:
ELISA can be used to measure produced antibodies or Trichinella spp. antigens in the samples. They are detected with antibodies linked to an enzyme that reacts with a substrate and generate a colour reaction. The optical density (OD) of the reaction is measured spectrophotometrically. ELISA assays can be done in several different procedures called „direct”, "indirect", "sandwich", and "competition" ELISA. Since the 1970s, the studies have been done on improving or replacing direct methods of Trichinella diagnosis with serological methods based on the ELISA. When somatic antigens of L1 T. spiralis were used, the specificity of the ELISA was poor due to a high probability of cross-reactions with other pathogens. During the 1980s the specificity of the ELISA was improved by excretory-secretory (E/S) antigens obtained during Trichinella muscle larvae incubation in vitro. Recently a synthetic glycan antigen has been developed and the increasing of ELISA specificity and sensitivity was noticed. The sensitivity of the ELISA using an E/S antigen ranging from 93.1 to 99.2% but the specificity from 90.6 to 99.4%. The ELISA method is relatively simple to apply, reliable, readily standardized and provides an acceptable balance of sensitivity and specificity. But all modified procedures should be validated. In Poland, the studies on the usefulness of ELISA for antibodies detection against T. spiralis in pigs and wild animals are limited. Own ELISA procedure was prepared in Pathophysiology Lab. in W. Stefański Institute of Parasitology of PAS. ELISA was used to examine IgG level against L1 T. spiralis in pigs and wild boars serum samples. Of 1474 pig samples, only 12 were positive. Of 1880 wild boar samples only 14 were positive. The results of this study are comparable with performance obtained using commercial sets. The results showed the usefulness of ELISA for T. spiralis diagnosis in pigs and wild boars and confirmed the possibility of use the ELISA test for application in the slaughterhouse.
Źródło:
Wiadomości Parazytologiczne; 2006, 52, 3; 205-212
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Przydatnosc ELISA do diagnozowania wlosnicy u swin i dzikow
The usefulness of ELISA for diagnosis of trichinellosis in pigs and wild boars
Autorzy:
Bien, J
Powiązania:
https://bibliotekanauki.pl/articles/840219.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
wlosnica
choroby pasozytnicze
diagnostyka weterynaryjna
Polska
walidacja
pasozyty
Warszawa konferencja
trzoda chlewna
dziki
wykorzystanie
parazytologia
konferencje
Trichinella
choroby zwierzat
test ELISA
wykrywanie
Opis:
ELISA can be used to measure produced antibodies or Trichinella spp. antigens in the samples. They are detected with antibodies linked to an enzyme that reacts with a substrate and generate a colour reaction. The optical density (OD) of the reaction is measured spectrophotometrically. ELISA assays can be done in several different procedures called „direct”, "indirect", "sandwich", and "competition" ELISA. Since the 1970s, the studies have been done on improving or replacing direct methods of Trichinella diagnosis with serological methods based on the ELISA. When somatic antigens of L1 T. spiralis were used, the specificity of the ELISA was poor due to a high probability of cross-reactions with other pathogens. During the 1980s the specificity of the ELISA was improved by excretory-secretory (E/S) antigens obtained during Trichinella muscle larvae incubation in vitro. Recently a synthetic glycan antigen has been developed and the increasing of ELISA specificity and sensitivity was noticed. The sensitivity of the ELISA using an E/S antigen ranging from 93.1 to 99.2% but the specificity from 90.6 to 99.4%. The ELISA method is relatively simple to apply, reliable, readily standardized and provides an acceptable balance of sensitivity and specificity. But all modified procedures should be validated. In Poland, the studies on the usefulness of ELISA for antibodies detection against T. spiralis in pigs and wild animals are limited. Own ELISA procedure was prepared in Pathophysiology Lab. in W. Stefański Institute of Parasitology of PAS. ELISA was used to examine IgG level against L1 T. spiralis in pigs and wild boars serum samples. Of 1474 pig samples, only 12 were positive. Of 1880 wild boar samples only 14 were positive. The results of this study are comparable with performance obtained using commercial sets. The results showed the usefulness of ELISA for T. spiralis diagnosis in pigs and wild boars and confirmed the possibility of use the ELISA test for application in the slaughterhouse.
Źródło:
Annals of Parasitology; 2006, 52, 3; 205-212
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The 20th International Conference of the World Association for the Advancement of Veterinary Parasitology, Christchurch, New Zealand
Autorzy:
Mizgajska-Wiktor, H.
Andrzejewska, I.
Powiązania:
https://bibliotekanauki.pl/articles/840345.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
zwalczanie pasozytow
Christchurch konferencja
pasozyty zwierzat
protozoologia weterynaryjna
pasozyty zewnetrzne
konferencje
inwazja pasozytnicza
parazytologia weterynaryjna
profilaktyka weterynaryjna
zwierzeta hodowlane
Źródło:
Annals of Parasitology; 2006, 52, 2; 141-142
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wpływ procedury otrzymywania antygenów ekskrecyjno-sekrecyjnych Trichinella spiralis na wynik testu ELISA u zarażonych świń
The influence of the procedure of excretory -secretory L1 Trichinella spiralis antigen preparation on the efficiency of an ELISA test in pigs
Autorzy:
Bień, J.
Powiązania:
https://bibliotekanauki.pl/articles/2144343.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
analiza statystyczna
diagnostyka weterynaryjna
pasozyty
nicienie
Warszawa konferencja
otrzymywanie
procedury
trzoda chlewna
zwierzeta chore
parazytologia
Trichinella spiralis
konferencje
antygeny
wartosc diagnostyczna
test ELISA
Opis:
Background. Trichinellosis is a parasitic zoonosis transmitted to humans by consumption of raw or undercooked meat from animals infected by worms of the Trichinella genus. Every year seropositive cases are found among the human population and thus trichinellosis still remains an epidemiologically important disease in Poland. The usefulness of ELISA for anti-T. spiralis IgG detection in pigs is still limited by the nature of antigen. The objective in the present study was to compare the usefulness of excretory-secretory antigens of L1 T. spiralis for the serological detection of IgG antibodies in pigs. Material and methods. The antigens were prepared in different laboratories: Ag ES L1 T. spiralis (N) in Germany, Ag ES L1 T. spiralis (W) in Italy and Ag ES L1 T. spiralis in Poland. Conventional, Iberian pigs were infected with 200, 1000 and 20 000 muscle larvae of T. spiralis. Serum samples were obtained at 5 and 1 dbi (day before infection), and 5, 10, 15, 20, 25, 30, 40, 50, 60 dpi (day post infection) and screened for specific IgG antibodies to excretory-secretory L1 T. spiralis antigens. Serum samples were obtained from the EU project TRICHIPORSE. The cut-off value of ELISA was determined on serum samples from 248 Trichinella-free pigs from Poznań and Boża Wola, that were examined by artificial digestion. Results. In pigs infected with 200 L1 T. spiralis larvae, specific IgG were detectable from 50 dpi, when the Ag ES L1 T. spiralis (N) was used, whereas when Ag ES L1T. spiralis (W) and Ag ES L1 T. spiralis were used, the specific IgG were detectable from 40 dpi. In pigs infected with 1000 L1 T. spiralis larvae, specific IgG was observed from 30 dpi when Ag ES L1 T. spiralis (W) and Ag ES L1 T. spiralis were used, but when Ag ES L1 T. spiralis (N) was used specific IgG were detectable from 40 dpi. In the group infected with the highest dose of T. spiralis larvae, specific IgG were detectable from 30 dpi when Ag ES L1 T. spiralis (N) and Ag ES L1 T. spiralis (W) were used, whereas when Ag ES L1 T. spiralis was used specific IgG were detectable from 20 dpi. The results strongly indicated that in the examined pigs, the specific IgG response against T. spiralis infection is dose dependent. Furthermore, it was shown that the high infectious dose induced earlier increasing of specific IgG response. Statistical analysis revealed a significant positive correlation between OD values obtained in procedures based on the three antigens. The results were statistically repeatable for procedures and for single pigs (P<0.01).
Źródło:
Wiadomości Parazytologiczne; 2006, 52, 3; 219-230
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wplyw procedury otrzymywania antygenow ekskrecyjno-sekrecyjnych Trichinella spiralis na wynik testu ELISA u zarazonych swin
The influence of the procedure of excretory -secretory L1 Trichinella spiralis antigen preparation on the efficiency of an ELISA test in pigs
Autorzy:
Bien, J
Powiązania:
https://bibliotekanauki.pl/articles/840241.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
analiza statystyczna
diagnostyka weterynaryjna
pasozyty
nicienie
Warszawa konferencja
otrzymywanie
procedury
trzoda chlewna
zwierzeta chore
parazytologia
Trichinella spiralis
konferencje
antygeny
wartosc diagnostyczna
test ELISA
Opis:
Background. Trichinellosis is a parasitic zoonosis transmitted to humans by consumption of raw or undercooked meat from animals infected by worms of the Trichinella genus. Every year seropositive cases are found among the human population and thus trichinellosis still remains an epidemiologically important disease in Poland. The usefulness of ELISA for anti-T. spiralis IgG detection in pigs is still limited by the nature of antigen. The objective in the present study was to compare the usefulness of excretory-secretory antigens of L1 T. spiralis for the serological detection of IgG antibodies in pigs. Material and methods. The antigens were prepared in different laboratories: Ag ES L1 T. spiralis (N) in Germany, Ag ES L1 T. spiralis (W) in Italy and Ag ES L1 T. spiralis in Poland. Conventional, Iberian pigs were infected with 200, 1000 and 20 000 muscle larvae of T. spiralis. Serum samples were obtained at 5 and 1 dbi (day before infection), and 5, 10, 15, 20, 25, 30, 40, 50, 60 dpi (day post infection) and screened for specific IgG antibodies to excretory-secretory L1 T. spiralis antigens. Serum samples were obtained from the EU project TRICHIPORSE. The cut-off value of ELISA was determined on serum samples from 248 Trichinella-free pigs from Poznań and Boża Wola, that were examined by artificial digestion. Results. In pigs infected with 200 L1 T. spiralis larvae, specific IgG were detectable from 50 dpi, when the Ag ES L1 T. spiralis (N) was used, whereas when Ag ES L1T. spiralis (W) and Ag ES L1 T. spiralis were used, the specific IgG were detectable from 40 dpi. In pigs infected with 1000 L1 T. spiralis larvae, specific IgG was observed from 30 dpi when Ag ES L1 T. spiralis (W) and Ag ES L1 T. spiralis were used, but when Ag ES L1 T. spiralis (N) was used specific IgG were detectable from 40 dpi. In the group infected with the highest dose of T. spiralis larvae, specific IgG were detectable from 30 dpi when Ag ES L1 T. spiralis (N) and Ag ES L1 T. spiralis (W) were used, whereas when Ag ES L1 T. spiralis was used specific IgG were detectable from 20 dpi. The results strongly indicated that in the examined pigs, the specific IgG response against T. spiralis infection is dose dependent. Furthermore, it was shown that the high infectious dose induced earlier increasing of specific IgG response. Statistical analysis revealed a significant positive correlation between OD values obtained in procedures based on the three antigens. The results were statistically repeatable for procedures and for single pigs (P<0.01).
Źródło:
Annals of Parasitology; 2006, 52, 3; 219-230
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wykorzystanie sekwencji genu rRNA w molekularnej diagnostyce parazytologicznej
Molecular diagnostic of parasites using rRNA gene sequence
Autorzy:
Dlugosz, E
Wisniewski, M.
Powiązania:
https://bibliotekanauki.pl/articles/841440.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
geny kodujace
diagnostyka parazytologiczna
diagnostyka weterynaryjna
wykorzystanie
parazytologia
konferencje
sekwencja genu
gen rRNA
Warszawa konferencja
Opis:
Ribosomal RNA (rRNA) is a component of the ribosomes. Eukaryotic ribosomes contain four different rRNA molecules: 18S, 5,8S, 28S and 5S rRNA. rRNA is the most conserved (least variable) gene in all cells. For this reason, genes that encode the rRNA (rDNA) are sequenced to identify an organism's taxonomic group, calculate related groups, and estimate rates of species divergence. Especially the internal transcribed spacers (ITS) are very useful for molecular diagnostic of parasite. They are noncoding regions of DNA sequence that separate genes coding for the 28S, 5.8S, and 18S ribosomal RNAs. These ribosomal RNA (rRNA) genes are highly conserved across taxa while the spacers between them may be species-specific. In this paper authors describe practical using of rRNA gene to parasite diagnostic.
Źródło:
Annals of Parasitology; 2006, 52, 4; 263-269
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wykorzystanie sekwencji genu rRNA w molekularnej diagnostyce parazytologicznej
Molecular diagnostic of parasites using rRNA gene sequence
Autorzy:
Długosz, E.
Wiśniewski, M.
Powiązania:
https://bibliotekanauki.pl/articles/2144424.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
geny kodujace
diagnostyka parazytologiczna
diagnostyka weterynaryjna
wykorzystanie
parazytologia
konferencje
sekwencja genu
gen rRNA
Warszawa konferencja
Opis:
Ribosomal RNA (rRNA) is a component of the ribosomes. Eukaryotic ribosomes contain four different rRNA molecules: 18S, 5,8S, 28S and 5S rRNA. rRNA is the most conserved (least variable) gene in all cells. For this reason, genes that encode the rRNA (rDNA) are sequenced to identify an organism's taxonomic group, calculate related groups, and estimate rates of species divergence. Especially the internal transcribed spacers (ITS) are very useful for molecular diagnostic of parasite. They are noncoding regions of DNA sequence that separate genes coding for the 28S, 5.8S, and 18S ribosomal RNAs. These ribosomal RNA (rRNA) genes are highly conserved across taxa while the spacers between them may be species-specific. In this paper authors describe practical using of rRNA gene to parasite diagnostic.
Źródło:
Wiadomości Parazytologiczne; 2006, 52, 4; 263-269
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Występowanie nicieni przewodu pokarmowego u lisów wolno żyjących w wojewodztwie zachodniopomorskim
Autorzy:
Pilarczyk, B.
Balicka-Ramisz, A.
Ramisz, A.
Powiązania:
https://bibliotekanauki.pl/articles/2146396.pdf
Data publikacji:
2005
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
wystepowanie
nicienie przewodu pokarmowego
woj.zachodniopomorskie
lisy
parazytologia weterynaryjna
zwierzeta dziko zyjace
Źródło:
Wiadomości Parazytologiczne; 2005, 51, 3; 249-251
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wystepowanie nicieni przewodu pokarmowego u lisow wolno zyjacych w wojewodztwie zachodniopomorskim
Autorzy:
Pilarczyk, B
Balicka-Ramisz, A.
Ramisz, A.
Powiązania:
https://bibliotekanauki.pl/articles/836570.pdf
Data publikacji:
2005
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
wystepowanie
nicienie przewodu pokarmowego
woj.zachodniopomorskie
lisy
parazytologia weterynaryjna
zwierzeta dziko zyjace
Źródło:
Annals of Parasitology; 2005, 51, 3; 249-251
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 87

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