- Tytuł:
- Kinetics of inactivation of glutamate decarboxylase by cysteine-specific reagents.
- Autorzy:
-
McCormick, Sara
Tunnicliff, Godfrey - Powiązania:
- https://bibliotekanauki.pl/articles/1044156.pdf
- Data publikacji:
- 2001
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
mercuric chloride
Escherichia coli
DTNB
cysteinyl residues
p-chloromercuribenzoate
glutamate decarboxylase
inactivation
sulfhydryl groups - Opis:
- Mercuric chloride, p-chloromercuribenzoate and 5,5'-dithiobis(2-nitrobenzoic acid) irreversibly inhibited the activity of Escherichia coli glutamate decarboxylase. Their second order rate constants for inactivation are 0.463 μM-1min-1, 0.034 μM-1min-1, 0.018 μM-1min-1, respectively. The characteristics of the inhibition by the three thiol-group reagents supports the idea that cysteinyl residues at the binding sites for the cofactor and/or the substrate are important for enzyme activity in E. coli.
- Źródło:
-
Acta Biochimica Polonica; 2001, 48, 2; 573-578
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł