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    Wyświetlanie 1-2 z 2
    Tytuł:
    The response of L5178Y lymphoma sublines to oxidative stress: Antioxidant defence, iron content and nuclear translocation of the p65 subunit of NF-κB.
    Autorzy:
    Boużyk, Elżbieta
    Grądzka, Iwona
    Iwaneńko, Teresa
    Kruszewski, Marcin
    Sochanowicz, Barbara
    Szumiel, Irena
    Powiązania:
    https://bibliotekanauki.pl/articles/1044205.pdf
    Data publikacji:
    2000
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    iron
    L5178Y murine lymphoma sublines
    NF-κB
    lovastatin
    sensitivity to hydrogen peroxide
    antioxidant defence
    oxidative stress
    Opis:
    We examined the response to hydrogen peroxide of two L5178Y (LY) sublines which are inversely cross-sensitive to hydrogen peroxide and X-rays: LY-R cells are radioresistant and hydrogen peroxide-sensitive, whereas LY-S cells are radiosensitive and hydrogen peroxide-resistant. Higher initial DNA breaks and higher iron content (potentially active in the Fenton reaction) were found in the hydrogen peroxide sensitive LY-R cells than in the hydrogen peroxide resistant LY-S cells, whereas the antioxidant defence of LY-R cells was weaker. In particular, catalase activity is twofold higher in LY-S than in LY-R cells. The content of monobromobimane-reactive thiols is 54% higher in LY-S than in LY-R cells. In contrast, the activity of glutathione peroxidase (GPx) is about two times higher in LY-R than in LY-S cells; however, upon induction with selenium the activity increases 15.6-fold in LY-R cells and 50.3-fold in LY-S cells. Altogether, the sensitivity difference is related to the iron content, the amount of the initial DNA damage, as well as to the efficiency of the antioxidant defence system. Differential nuclear translocation of p65-NF-κB in LY sublines is due to the more efficient antioxidant defence in LY-S than in LY-R cells.
    Źródło:
    Acta Biochimica Polonica; 2000, 47, 4; 881-888
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Ozone effects on trees, where uptake and detoxification meet
    Autorzy:
    De, Temmerman L
    Vandermeiren, K.
    D'Haese, D.
    Bortier, K.
    Asard, H.
    Ceulemans, R.
    Powiązania:
    https://bibliotekanauki.pl/articles/41371.pdf
    Data publikacji:
    2002
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    air pollutant
    ozone effect
    oxidative defence
    detoxification
    ozone
    critical level
    tree
    ozone concentration
    effective ozone flux
    Opis:
    Ozone is the most important air pollutant and its concentration in ambient air is still rising. Ozone concentrations measured at reference height (50 m is EMEP ozone modelling height), do not reflect the real concentration at the top of the vegetative canopy and do not provide sufficient information about the ozone fluxentering the leaves. Modelling stomatal conductance is leading to estimations of cumulative ozone uptake and enables much better to evaluate the impact of ozone on trees. The negative impact of ozone exposure has a measurable effect on physiological processes such as stomatal conductance, photosynthesis and respiration. Disturbance of the basic physiological processes is leading to growth and wood production losses. There have been several attempts to establish critical levels (CL) for ozone effects on forest trees. Average concentrations and cumulative exposure indices are satisfactory to some extent, but do not fully describe the potential impact of ozone exposure. Much more promising is an evaluation based on the effective ozone flux, which is a function of the absorbed ozone flux and the defensive response. Ozone uptake takes place primarily through the stomata and reactions of ozone with hydrocarbons released by the plant cells and transformations of dissolved ozone in the apoplastic fluid create many reactive oxygen species of which free radicals are able to initiate membrane lipid peroxidation and destruction of cell membranes. The defence of a plant against absorbed ozone starts in the apoplastic fluid. Ascorbate is believed to be a very important radical scavenger avoiding detrimental effects of reactive oxygen species to the membranes. Other important antioxidants are phenolics. The defensive response can be linked to the abundance of ascorbate or the ability of the plants to regenerate (reduce) ascorbate from monodehydroascorbate and dehydroascorbate. The reduction of dehydroascorbate takes place in the symplast where ascorbate can be transported back through the plasma membrane into the apoplast. Ozone exposure also causes oxidative stress of the plant cell interior by the formation of reactive oxygen species. Plants can cope with those toxic substances in the symplast by using antioxidants such as ascorbate, -tocopherol, glutathione and carotenoids and enzymes such as superoxide dismutases, catalases and several peroxidases. The complexity of the apoplastic and symplastic antioxidative capacity with different turnover rates and transport of antioxidants makes it difficult to determine the total antioxidative power.
    Źródło:
    Dendrobiology; 2002, 47
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-2 z 2

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