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Wyświetlanie 1-10 z 17
Tytuł:
Multidrug resistance-associated protein - reduction of expression in human leukaemia cells by antisense phosphorothioate olignucleotides.
Autorzy:
Niewiarowski, Wojciech
Gendaszewska, Edyta
Rębowski, Grzegorz
Wójcik, Marzena
>Mikołajczyk, Barbara
Soszyński, Mirosław
Bartosz, Grzegorz
Powiązania:
https://bibliotekanauki.pl/articles/1044273.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
antisense oligonucleotides
MRP
multidrug resistance
Opis:
Multidrug resistance-associated protein (MRP1) causes cellular drug resistance in several cancer cell lines. In this paper we show that antisense oligonucleotides decrease MRP1 expression in human leukaemia cells. We investigated biological activity of a series of 12 linear phosphorothioate oligonucleotides, complementary to several regions of MRP1 mRNA. The oligonucleotides were administered to leukaemia HL60/ADR cells overexpressing MRP1 protein. Then, the level of MRP1 mRNA was determined by means of semiquantitative RT-PCR and the protein level by reaction with specific monoclonal antibodies. Some of the investigated antisense oligonucleotides decrease the expression level of the MRP1 protein by 46% and its mRNA level by 76%.
Źródło:
Acta Biochimica Polonica; 2000, 47, 4; 1183-1188
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
RNA modulation, repair and remodeling by splice switching oligonucleotides.
Autorzy:
Kole, Ryszard
Williams, Tiffany
Cohen, Lisa
Powiązania:
https://bibliotekanauki.pl/articles/1043268.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
RNA splicing; antisense oligonucleotides; genetic disease
Opis:
Targeting splicing by antisense oligonucleotides allows RNA modifications that are not possible with RNA interference or other antisense techniques that destine the RNA for destruction. By changing the ratio of naturally occurring splice variants the expression of mRNA is modulated. By preventing the use of an aberrant splice site created by a mutation and enforcing re-selection of correct splice sites the RNA is repaired. Antisense induced skipping of the exon that carries a nonsense mutation remodels the mRNA and restores the reading frame of the defective protein. All of the above approaches have clinical applications. Modulation of splice variants is particularly important since close to 60% of all genes code for alternatively spliced pre-mRNA.
Źródło:
Acta Biochimica Polonica; 2004, 51, 2; 373-378
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Civilization diseases – therapeutic application of antisense strategy
Autorzy:
Bąkowska-Żywicka, Kamila
Tyczewska, Agata
Twardowski, Tomasz
Powiązania:
https://bibliotekanauki.pl/articles/703440.pdf
Data publikacji:
2008
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
civilization diseases
antisense oligonucleotides
novel therapies
Opis:
Modulation and regulation of biosynthesis process by means of antisense oligonucleotides lay the foundation for new therapeutic strategies, among others against cancer, viral or angiogenesis diseases. The aim of this account is the presentation of molecular basis of antisense strategy and mechanism of action of antisense oligonucleotides. Furthermore we present the state of art in clinical studies of application of antisense oligonucleotides in some of civilization diseases.
Źródło:
Nauka; 2008, 1
1231-8515
Pojawia się w:
Nauka
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Tissue distribution of a menthyl-conjugated oligodeoxyribonucleotide antisense to PAI-1 mRNA
Autorzy:
Szemraj, Janusz
Al-Nedawi, Khalid
Chabielska, Ewa
Buczko, Wlodzimierz
Pawlowska, Zofia
Powiązania:
https://bibliotekanauki.pl/articles/1041329.pdf
Data publikacji:
2005
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
PAI-1
antisense oligonucleotides
tissue distribution
Opis:
The inhibitory effect of numerous analogues of PO-16, an hexadecadeoxyribonucleotide antisense to sequences -22 to -17 of PAI-1 mRNA coding for a fragment of the signal peptide, on the expression of PAI-1 in endothelial cells, and physiological consequences of the subsequently reduced PAI-1 activity tested in vitro and in vivo, were described in our previous studies. Of particular interest was PO-16 5'-O-conjugated with menthyl phosphorothioate (MPO-16R). In this work, tissue localisation of MPO-16R labelled with [35S] phosphorothioate at the 3'-end, was determined. [35S]MPO-16R and control [35S]MPO-16R-SENSE oligonucleotides were administered intravenously into 22 rats and organ distribution of the labelled bioconjugates was assessed after 24 and 48 h. For this purpose, tissue sections were subjected to autoradiography, and quantitated by liquid scintillation after solubilisation. Overall clearance of radioactivity was already seen after 24 h, with the radioactivity recovered mainly in the kidney and liver. A smaller fraction of radioactivity was also retained in the spleen and heart. The kidney concentration of the labelled probe was higher than that of liver by 50%. The distribution of PAI-1 mRNA in untreated rat kidney, liver, spleen and heart established by two independent techniques: Ribonuclease Protection Assay and Real-Time PCR, shows the same pattern as that observed for [35S]MPO-16R antisense.
Źródło:
Acta Biochimica Polonica; 2005, 52, 4; 849-855
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Od syntezy oligonukleotydów do Mycoplasma laboratorium
Autorzy:
Laskowski, M.
Powiązania:
https://bibliotekanauki.pl/articles/273706.pdf
Data publikacji:
2011
Wydawca:
Roble
Tematy:
biologia syntetyczna
oligonukleotydy
Mycoplasma laboratorium
synthetic biology
oligonucleotides
Źródło:
LAB Laboratoria, Aparatura, Badania; 2011, 16, 3; 22-25
1427-5619
Pojawia się w:
LAB Laboratoria, Aparatura, Badania
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Oligodeoxyadenylate stimulates the protein kinase activity of anti-DNA sIgA from human milk.
Autorzy:
Kit, Yuri
Kuligana, Elena
Semenov, Dimitry
Richter, Vladimir
Powiązania:
https://bibliotekanauki.pl/articles/1043844.pdf
Data publikacji:
2002
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
anti-DNA antibodies
abzymes
protein kinase activity
oligonucleotides
human milk
Opis:
Preparations of anti-DNA sIgA were obtained from human milk by sequential chromatography on protein A-sepharose, DEAE-fractogel and DNA-cellulose. The influence of oligonucleotides on protein kinase activity was investigated. It was discovered that incubation of anti-DNA sIgA with oligodeoxyriboadenylate d(A)12 stimulates the phosphorylation of polypeptides of sIgA in the presence of [γ-32P]ATP. The greatest was the incorporation of 32P into the sIgA H-chains. We also demonstrated stimulation of the casein kinase activity of anti-DNA sIgA by d(A)12. The stimulation of the protein kinase activity of anti-DNA sIgA by oligoriboadenylate r(A)12 was not detected.
Źródło:
Acta Biochimica Polonica; 2002, 49, 1; 291-294
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Adsorbenty ze związanymi cieczami jonowymi i ich wykorzystanie w przygotowaniu próbek oligonukleotydów
Adsorbents with bonded ionic liquids and their use in the preparation of oligonucleotides samples
Autorzy:
Nuckowski, Łukasz
Zalesińska, Ewa
Studzińska, Sylwia
Powiązania:
https://bibliotekanauki.pl/articles/172150.pdf
Data publikacji:
2020
Wydawca:
Polskie Towarzystwo Chemiczne
Tematy:
przygotowanie próbek
oligonukleotydy
ciecze jonowe
adsorbenty
sample preparation
oligonucleotides
ionic liquids
adsorbents
Opis:
Oligonucleotides are short fragments of nucleic acids. They have a growing potential in medicine, especially as diagnostic and therapeutic agents. In most cases, these compounds are determined in the complex biological matrix. Thus, the sample preparation step is very important in their bioanalysis. Solid-phase extraction is a predominant technique in this field. However, presently used for this purpose adsorbents have disadvantages. They ensure low extraction effectiveness and procedures using them are labor-intensive or time-consuming. Ionic liquids, since their discovery, are objects of intensive interest of scientists. Their scientific attractiveness is connected with their unique properties. They are used in separation and sample preparation techniques, such as liquid-liquid extraction using water-immiscible ionic liquids. This approach was also used in the extraction of oligonucleotides. Adsorbents modified with ionic-liquids have growing potential in extraction techniques. Few types of materials are used, namely carbon, polymers, and silica. A common feature of these materials modified with ionic liquids is the ion exchange character. Nonetheless, carbon nanomaterials are coated or covalently modified with ionic liquids, and they are used mainly for nonpolar compounds. Polymer and silica-based adsorbents are used mainly for acidic compounds. Polymers are characterized by the highest stability of the presented materials. Due to their ion-exchange properties crosslinked poly(ionic liquids) were used also for extraction of unmodified and modified oligonucleotides. The optimized procedure applying the material with bonded zwitterion ionic liquid gives high recoveries. It is concurrent for presently used adsorbents, thus solves problems connected with their usage. Moreover, it can be used for biological samples without any pre-purification.
Źródło:
Wiadomości Chemiczne; 2020, 74, 7-8; 545-565
0043-5104
2300-0295
Pojawia się w:
Wiadomości Chemiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Topical treatment of LdMNPV-infected gypsy moth caterpillars with 18 nucleotides long antisense fragment from LdMNPV IAP3 gene triggers higher levels of apoptosis in infected cells and mortality of the pest
Autorzy:
Oberemok, Volodymyr V.
Laikova, Kateryna V.
Zaitsev, Alexey S.
Nyadar, Palmah M.
Gninenko, Yuri I.
Gushchin, Vladimir A.
Makarov, Valentin V.
Agranovsky, Alexey A.
Powiązania:
https://bibliotekanauki.pl/articles/961638.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
baculoviral infection
dna insecticides
dna oligonucleotides
forest and
crop protection
gypsy moth
iap genes
Opis:
The high efficiency of baculovirus infection is partially explained by the ability of the virus to suppress host defense machinery connected with the apoptosis pathway. Members of the baculovirus gene family, inhibitors of apoptosis (vIAPs), have been shown to inhibit apoptosis in baculovirus-infected cells. Here we showed that treatment of the LdMNPV- -infected 1st instar gypsy moth (Lymantria dispar) caterpillars with sense (oligoBIR) and antisense (oligoRING) DNA oligonucleotides from the LdMNPV IAP3 gene induced elevated mortality of the insects. Apoptotic DNA ladder assay showed that the leading role in this phenomenon is played by the antisense oligoRING fragment of the vIAP3 gene. These results imply that the application of both antisense DNA oligonucleotides from vIAP genes and baculovirus preparations (one following the other) may be a potential method for plant protection against insect pests.
Źródło:
Journal of Plant Protection Research; 2017, 57, 1
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Chromatograficzna analiza związków budujących kwasy nukleinowe
Chromatographic analysis of nucleic acids constituents
Autorzy:
Studzińska, S.
Rola, R.
Łobodziński, F.
Krzemińska, K.
Powiązania:
https://bibliotekanauki.pl/articles/172180.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Chemiczne
Tematy:
nukleozydy
nukleotydy
oligonukleotydy
chromatografia cieczowa
czułość
selektywność
nucleosides
nucleotides
oligonucleotides
liquid chromatography
sensitivity
selectivity
Opis:
Understanding the characteristics, role and structure of nucleic acids allowed to answer questions about the disease processes. Today, nucleic acids and their constituents are tools, which are used by molecular biology in medicine and biotechnology. Antisense and gene therapy are intensively developing methods for possible treating or preventing disease. They use short fragments of DNA or RNA - oligonucleotides to silence the genes expression. They are not the only ones that allow analytical chemists to obtain information about the state of our body. Determination of modified nucleoside allows detection of cancer, while analysis of nucleotides allows the estimation of strengthening the immune system. There is a great need of sensitive, selective and precise methods of separation of nucleosides, nucleotides and oligonucleotides and their qualitative and quantitative analysis. Consequently liquid chromatography (LC) is the most commonly used for analysis of nucleic acid constituents. The most widely used modes of LC include Ion Exchange Chromatography (IEC) and Reversed Phase High Performance Liquid Chromatography (RP HPLC). Both techniques have their advantages and disadvantages in the analysis of nucleosides, nucleotides and oligonucleotides. In the case of IEC it is necessary to use high concentrations of the salt in the mobile phase or concentration gradients, which considerably limits the possibility of using MS detection. RP HPLC can be coupled with MS detection but only when volatile salts are mobile phase components. On the other hand there is a significant problem is the lack of sufficient selectivity for the most polar nucleosides and nucleotides. RP HPLC MS is still most often used in the determination of nucleosides and nucleotides, due to its high sensitivity and a comprehensive qualitative analysis. Another system used for the HPLC analysis of oligonucleotides is Ion Pair Reversed Phase High Performance Liquid Chromatography (IP RP HPLC). These compounds can not be analyzed by RP HPLC due to their high polarity. The advantage of IP RP HPLC is selectivity, achieved by a suitable choice of mobile phase composition and the possibility of using MS. A disadvantage of IP RP HPLC in the analysis of oligonucleotides is however lower sensitivity compared to RP HPLC. During the last few years Hydrophilic Interaction Liquid Chromatography (HILIC) was applied for the separation of mixtures of nucleosides, nucleotides, oligonucleotides extracted from a biological or food samples. The presented results demonstrate the usefulness of this method, however, the resolving power is limited due to the asymmetric peak shape. On the other hand proper selection of the mobile and stationary phase can lead to a high selectivity in the analysis of the most polar nucleosides, nucleotides and oligonucleotides, which can not be separated by RP HPLC.
Źródło:
Wiadomości Chemiczne; 2016, 70, 9-10; 633-656
0043-5104
2300-0295
Pojawia się w:
Wiadomości Chemiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A breakthrough in the efficiency of contact DNA insecticides: rapid high mortality rates in the sap-sucking insects Dynaspidiotus britannicus Comstock and Unaspis euonymi Newstead
Autorzy:
Gal`chinsky, N.
Useinov, R.
Yatskova, E.
Laikova, K.
Novikov, I.
Gorlov, M.
Trikoz, N.
Sharmagiy, A.
Plugatar, Y.
Oberemok, V.
Powiązania:
https://bibliotekanauki.pl/articles/2082776.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
antisense oligonucleotides
DNA insecticides
insect pest control
sap-sucking
insects
28S ribosomal RNA
Opis:
In this short communication describing experiments carried out on the larvae of two insects, Unaspis euonymi Comstock (feeding on Euonymus japonicus Thunb.) and Dynaspidiotus britannicus Newstead (feeding on Laurus nobilis L.), we evaluate for the first time the efficiency of using DNA insecticides in the control of sap-sucking insects, including armored scale insects. Over a period of 10 days, high insect mortality was detected in both U. euonymi and D. britannicus, accompanied by a significant decrease in the concentration of target RNAs. At the same time, no visible changes were observed when the leaves of the host plants were subjected to treatment with DNA insecticides for one month. The results show the high efficiency of DNA insecticides used against hemipteran insect pests. It is noteworthy that the high efficiency of DNA insecticides and their low cost in comparison with RNA preparations provides a safe and extremely promising potential vehicle for the control of sap-sucking insects.
Źródło:
Journal of Plant Protection Research; 2020, 60, 2; 220-223
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
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