- Tytuł:
- Deuterium isotope effects in oxidation of dopamine and norepinephrine catalyzed by horseradish peroxidase
- Autorzy:
-
Byszewska, W.
Pająk, M.
Kańska, M. - Powiązania:
- https://bibliotekanauki.pl/articles/146852.pdf
- Data publikacji:
- 2013
- Wydawca:
- Instytut Chemii i Techniki Jądrowej
- Tematy:
-
deuterium
dopamine (DA)
norepinephrine
isotope effects
oxidation
peroxidase - Opis:
- Deuterium kinetic isotope effects (KIE’s) in the reaction of oxidation of dopamine (DA) catalyzed by the enzyme horseradish peroxidase (HRP, EC 1.11.1.7) was determined using a non-competitive spectrophotometric method. For kinetic studies, the deuterium ring labelled isotopomer [2’,5’,6’-2H3]-dopamine was synthesized using acid catalyzed isotopic exchange between authentic dopamine and heavy water. Deuterium solvent isotope effects (SIE’s) for dopamine and norepinephrine were determined separately for the enzymatic oxidation carried out in the presence or absence of hydrogen peroxide. Some mechanistic details of enzymatic oxidation of dopamine and norepinephrine to corresponding catecholchromes catalyzed by HRP were discussed.
- Źródło:
-
Nukleonika; 2013, 58, 4; 475-479
0029-5922
1508-5791 - Pojawia się w:
- Nukleonika
- Dostawca treści:
- Biblioteka Nauki
Artykuł