- Tytuł:
-
Nienaturalne aminokwasy jako strategia do otrzymywania substratów, inhibitorów i niskocząsteczkowych sond aktywności dla enzymów proteolitycznych
Unnatural amino acids as achemical tool for the development of protease substrates, inhibitors and activity - baseproblems - Autorzy:
-
Poręba, Marcin
Kasperkiewicz-Wasilewska, Paulina
Rut, Wioletta
Drąg, Marcin - Powiązania:
- https://bibliotekanauki.pl/articles/2200587.pdf
- Data publikacji:
- 2022
- Wydawca:
- Polskie Towarzystwo Chemiczne
- Tematy:
-
proteolytic enzymes
substrate specificity
unnatural amino acids
substrates
inhibitors
activity-based probes
caspases
cathepsins
neuthrophil serine proteases
proteasome
SARS-CoV-2 proteases
enzymy proteolityczne
specyficzność substratowa
nienaturalne aminokwasy
niskocząsteczkowe sondy aktywności
kaspazy
katepsyny
proteasom
proteazy wirusa SARS-CoV-2 - Opis:
- Proteolytic enzymes are molecular scissors that are responsible for the amide bond breakdown in peptide and protein substrates. Over the years, the view on proteases has been considerably changed from non-specific digestive enzymes to sophisticated biocatalysts, which by performing limited proteolysis control virtually all biological processes. In order to better understand how proteases work and what are their biologically relevant target substrates, it is indispensable to determine their catalytic preferences. This knowledge can be further utilized to develop selective substrates, inhibitors and activity-based probes (ABPs) enabling the monitoring of proteases activity in various settings, from in vitro analysis on recombinant enzymes or cell lysates to ex vivo and in vivo imaging at the single cell level. Among many chemical-based approaches that have been developed and applied over the years, the Hybrid Combinatorial Substrate Library (HyCoSuL) technology has emerged as one of the most powerful one. HyCoSuL is a combinatorial peptide-based library of fluorogenic substrates, that comprise natural and unnatural amino acids, that can deeply explore the chemical space in proteases active site, providing a structural framework for the development of highly-selective chemical tools. In this review we present the most prominent examples of proteolytic enzymes that have been profiled with HyCoSuL approach yielding selective substrates, potent inhibitors, and very sensitive activity-based probes.
- Źródło:
-
Wiadomości Chemiczne; 2022, 76, 5-6; 433--454
0043-5104
2300-0295 - Pojawia się w:
- Wiadomości Chemiczne
- Dostawca treści:
- Biblioteka Nauki
Artykuł