Temat: nested-PCR - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Detection and Prevalence of white spot syndrome disease (WSSV) in shrimp farms
Autorzy:: Amarakoon, A. A. D. Gayathri U.
Wijegoonawardane, P. K. M.
Wicraamasinghe, W. A. L.
Powiązania:: https://bibliotekanauki.pl/articles/1188101.pdf
Data publikacji:: 2016
Wydawca:: Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:: WSSV
DNA genome
OIE
Penaeid shrimps
Nested PCR
Opis:: White spot syndrome is a viral infection of Penaeus monodon shrimp. It is highly lethal, contagious and killing shrimps and doesn’t have a specific anti-viral treatment for this infection. This important infection affects on shrimp farms in Sri Lanka and leads to the loss of economy. Most of shrimp products are export oriented. Therefore this study aimed to determine the factors, detection and prevalence of WSSV in shrimp farms, Sri Lanka. The prevalence rate of WSSV among the collected samples (n = 100) is 58.05% (2011), 59.06% (2012) in Sri Lanka respectively. Therefore the infection and resistance of virus has increased among the shrimp population annually. Further studies need to be identified the WSSV aggressive viral strains and prevention strategies to evade from this sever infection in shrimp farming.
Źródło:: World Scientific News; 2016, 56; 239-251
2392-2192
Pojawia się w:: World Scientific News
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Molecular identification of Giardia duodenalis isolates from domestic dogs and cats in Wroclaw, Poland
Autorzy:: Piekarska, Jolanta
Bajzert, Joanna
Gorczykowski, Michał
Kantyka, Magdalena
Podkowik, Magdalena
Powiązania:: https://bibliotekanauki.pl/articles/990993.pdf
Data publikacji:: 2016
Wydawca:: Instytut Medycyny Wsi
Tematy:: giardia duodenalis
assemblage
dogs
cats
nested-pcr
pcr-rflp
zoonosis
Opis:: Introduction. Giardia duodenalis (G. intestinalis) is a common protozoan causing gastrointestinal disorders in many species of mammals. The genus of Giardia has high molecular diversity. Dogs and cats, in addition to their typical infection with assemblages C, D and F, may be a reservoir of zoonotic assemblages (A and B). Objective. The aim of this study was a genetic characteristic of Giardia isolates of dogs and cats from the area of Wroclaw (Poland). Materials and method. A total of 128 and 33 faecal samples from dogs and cats, respectively, were analyzed by routine coprological methods. The animals were diagnosed on the presence of G. duodenalis antigens in faeces soluble with the use of SNAP Giardia (IDEXX Laboratories) immunosorbent assay. 27 DNA isolates of Giardia were subjected to molecular identification (PCR-RFLP). Results and conclusions. The prevalence of G. duodenalis was 21.1% (27/128) in dogs and 15.1% (5/33) in cats. In dogs, C assemblage was present in 18 (81%) positive stool samples, D assemblage in 2 (9%) samples, B assemblage present in one (4.5%), and mixed assemblages (C and D) occurred in one (4.5%) sample. F assemblage was found in 4 (80%) cats’ positive stool samples and A assemblage occurred in one case (20%). Confirmation of the presence of A and B zoonotic assemblages suggests that infected pets can be a threat to human health. This study describes for the first time the presence of mixed infections within host-specific C and D assemblages in dogs in Poland.
Źródło:: Annals of Agricultural and Environmental Medicine; 2016, 23, 3
1232-1966
Pojawia się w:: Annals of Agricultural and Environmental Medicine
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Toxoplasma gondii infection in selected species of free-living animals in Poland
Autorzy:: Sroka, J.
Karamon, J.
Wójcik-Fatla, A.
Dutkiewicz, J.
Bilska-Zając, E.
Zając, V.
Piotrowska, W.
Cencek, T.
Powiązania:: https://bibliotekanauki.pl/articles/2085130.pdf
Data publikacji:: 2019
Wydawca:: Instytut Medycyny Wsi
Tematy:: Polska
Toxoplasma gondii
nested PCR
genotyping
free-living animals
Opis:: Introduction and objective. Free-living animals can play an important role as a reservoir of Toxoplasma gondi;, however, data concerning this issue in Poland are still limited.The aim of study was to assess the occurrence of T. gondii infection by using molecular methods in free-living animals in selected regions of Poland. Materials and method. Tissues samples of 396 animals (foxes, muskrats, birds, martens, badgers, polecats, raccoons, minks, raccoon dogs, otters, small rodents and insectivores, and grass snakes were collected from various regions of Poland. After samples digestion, DNA was isolated using QIAmp DNA Mini Kit (Qiagen). DNA extraction from small rodents and insectivores samples was performed without digestion. Next, nested PCR (B1 gene) and, for a part of nested PCR positive amplicons, RFLP PCR, were performed according to the method by Grigg and Boothroyd (2001). The other part of nested PCR positive DNA isolates were genotyped using 5 genetic markers: SAG1, SAG2 (5’- and 3’), SAG3, BTUB and GRA6, based on the method by Dubey et al. (2006). These PCR products were sequenced and compared with the NCBI database using Blast. Results. In total, in 50 of the 396 examined animals DNA of T. gondii was detected (12.6%). The highest percentages of positive results in PCR was obtained in martens (40.9%) and badgers (38.5%), lower in birds (27.3%) and the lowest in foxes (7.4%). The RFLP and multilocus PCR analysis showed the dominance of T. gondii clonal type II (or II/III). Conclusions. The results of this study indicate the frequent T. gondii infection among free-living animals in Poland, especially martens and badgers, which may indirectly indicate that these animals contribute to the spread of the parasite in the sylvatic environment in Poland. The genotyping analysis showed the dominance of T. gondii clonal type II (or II/III).
Źródło:: Annals of Agricultural and Environmental Medicine; 2019, 26, 4; 656-660
1232-1966
Pojawia się w:: Annals of Agricultural and Environmental Medicine
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Cryptosporidium parvum – zoonotic subtype IIdA15G1 in a Slovakian patient
Autorzy:: Mravcova, K.
Strkolcova, G.
Mucha, R.
Barbusinova, E.
Goldova, M.
Kacirova, J.
Madar, M.
Powiązania:: https://bibliotekanauki.pl/articles/2085903.pdf
Data publikacji:: 2020
Wydawca:: Instytut Medycyny Wsi
Tematy:: Slovakia
nested PCR
Cryptosporidium spp
zoonotic subtype
veterinary student
Opis:: Introduction and objectives. The parasite Cryptosporidium spp. is an intracellular protozoa which has a broad range of hosts and zoonotic potential. It presents a serious health risk for agricultural workers and veterinarians. The aim of the study was to identify the species and subtypes of Cryptosporidium occurring in a veterinary student who came into contact with calves on a farm. Materials and method. The Ziehl-Neelsen staining technique was employed to confirm the presence of Cryptosporidium oocysts. ELISA test was applied to detect coproantigen in faecal specimens. Nested PCR was used to amplify a small ribosomal subunit (SSU rRNA) and sequencing of the GP60 gene served to identify the zoonotic subtypes. Results. The nested PCR allowed to confirm the C. parvum species; subsequently, the IIdA15G1 zoonotic subtype was identified. Conclusion. This is the first confirmed case in Slovakia of human cryptosporidiosis caused by the unique subtype IIdA15G1.
Źródło:: Annals of Agricultural and Environmental Medicine; 2020, 27, 3; 485-488
1232-1966
Pojawia się w:: Annals of Agricultural and Environmental Medicine
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: Optimization of flotation, DNA extraction and PCR methods for detection of Toxoplasma gondii oocysts in cat faeces
Autorzy:: Sroka, J.
Karamon, J.
Dutkiewicz, J.
Wójcik-Fatla, A.
Cencek, T.
Powiązania:: https://bibliotekanauki.pl/articles/2081971.pdf
Data publikacji:: 2018
Wydawca:: Instytut Medycyny Wsi
Tematy:: flotation
Toxoplasma gondii
faeces
Real time PCR
nested PCR
cats
oocysts detection
Opis:: Introduction and objective. The aim of the study was to compare the effectiveness of selected oocysts concentration methods, DNA extraction protocols and PCR assays targeting the B1 gene, for the development of procedures which would be effective and useful in laboratory practice for the detection of T. gondii in faecal samples from cats. Materials and method. In order to compare the influence of the flotation fluids on microscopy and PCR detection of T. gondii, saturated solutions of saccharose, MgSO4, ZnSO4 and NaNO3 were used. To determine the sensitivity of PCR tests used: Real time PCR (RT) and nested PCR, water samples spiked with T. gondii tachyzoites and oocysts were tested. DNA was extracted using DNeasy Blood and Tissue Kit (Qiagen) (K1). The same PCR tests were used to assess the efficacy of T. gondii DNA detection in samples of cat faeces spiked with oocysts, using DNA extraction by a K1 set and a K2 set (QIamp DNA Stool Mini Kit, (Qiagen). Results. The initial results showed that NaNO3 was most useful as a flotation fluid due to the lack negative effect on the oocysts and amplification efficacy in PCR. The level of detection for water samples (100 μl) was determined as 100 tachyzoites and 1–50 oocysts in RT, and 2–20 oocysts in nested PCR. The limit of detection (LD) for stool samples (250 mg) spiked with oocysts, where the K1 set was used, determined as 250 and 5 oocysts in RT and nested PCR, respectively. For samples extracted with the K2 set, LD in RT was determined as 1–50 oocysts (depending on the variant) and 50 oocysts in nested PCR. Conclusions. The most effective methods for detection of T. gondii in cat faeces seem to be centrifugal flotation with NaNO3, followed by DNA extraction with removing of inhibitors (K2 set) and Real Time PCR targeting B1 gene.
Źródło:: Annals of Agricultural and Environmental Medicine; 2018, 25, 4; 680-685
1232-1966
Pojawia się w:: Annals of Agricultural and Environmental Medicine
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 6.

Tytuł:: Detekcja fitopatogenów z rodzaju Phytophthora w glebach leśnych za pomocą analiz DNA
Detection of Phytophthora in forest soils using DNA analysis
Autorzy:: Kubiak, K.A.
Oszako, T.
Jabłoński, T.
Powiązania:: https://bibliotekanauki.pl/articles/996874.pdf
Data publikacji:: 2012
Wydawca:: Polskie Towarzystwo Leśne
Tematy:: lesnictwo
gleby lesne
czynniki chorobotworcze
mikroorganizmy glebowe
Phytophthora
identyfikacja
analiza DNA
metoda nested-PCR
phytophthora
identyfication
dna isolation
forest soil
pcr
Opis:: Pathogenic oomycetes from the genus Phytophthora attack the base trunks and root systems of trees causing their illness and dying. Under favorable conditions, they may cause damage to even 90% of fine roots. For this reason, they are a particular threat to seedlings in nurseries of forest trees and ornamental plants. Early detection and identification of Phytophthora species is a key issue in forest protection. The aim of this study was to compare the two methods of identification of pathogenic Phytophthora in soil samples collected around 50 selected oaks in Krotoszyn and Karczma Borowa forest districts. Each of the soil samples were analyzed in parallel: 1) direct isolation of genomic DNA from soil and 2) isolation of genomic DNA from soil, preceded by its three day pre−incubation in a selective medium for Phytophthora. Species identification was performed by PCR amplification with primers specific for the species of Phytophthora. The results indicate that the use of pre−incubation phase of soil in a medium−PARP PeaBroth before isolation of DNA, increases the sensitivity of detection of these phytopathogens using PCR. With pre−incubation, the method revealed 54% of positive findings, while simultaneously conducted the same analysis of soil samples by using only direct DNA isolation from soil and PCR amplification provided only 30% of positive findings.
Źródło:: Sylwan; 2012, 156, 06; 437-443
0039-7660
Pojawia się w:: Sylwan
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 7.

Tytuł:: NESTED PCR w diagnostyce przypadkow pneumocystozowego zapalenia pluc [PCP] u pacjentow z Aids
Autorzy:: Gołąb, E.
Sobolewska, A.
Bitkowska, E.
Bednarska, A.
Berak, H.
Dzbeński, T.H.
Horban, A.
Powiązania:: https://bibliotekanauki.pl/articles/2148066.pdf
Data publikacji:: 2001
Wydawca:: Polskie Towarzystwo Parazytologiczne
Tematy:: zapalenie pluc
metody diagnostyczne
czynniki chorobotworcze
choroby czlowieka
parazytologia lekarska
lancuchowa reakcja polimerazy
pneumocystoza
diagnostyka
AIDS
ludzie chorzy
Pneumocystis carinii
metoda nested-PCR
Opis:: The studies were undertaken to evaluate the usefulness of a nested PCR assay in the diagnosis of Pneumocystis carinii pneumonia (PCP) in AIDS patients. To achieve the end, 51 excretions samples from the respiratory traci were collected from HIV-infected patients with respiratory symptoms, and examined for the presence of specific DNA. A portion of the mitochondrial large-subunit rRNA gene of Pneumocystis carinii was amplified with outer primers pair pAZ 102E, pAZ 102H and internal primers pAZ 102X, pAZ 102Y. Positive nested PCR results were obtained with 36 out of 51 examined samples. Some 52.8% of the positive results were obtained with samples collected from patients without clinical diagnosis of PCP. It was concluded, that the nested PCR method, being too sensitive, is not suitable for the routine diagnosis of PCP in AIDS patients.
Źródło:: Wiadomości Parazytologiczne; 2001, 47, 3; 521-525
0043-5163
Pojawia się w:: Wiadomości Parazytologiczne
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 8.

Tytuł:: Comparison of PCR-DGGE and Nested-PCR-DGGE Approach for Ammonia Oxidizers Monitoring in Membrane Bioreactors’ Activated Sludge
zalety i wady wykorzystywania techniki nested-PCR w monitoringu bakterii utleniających amoniak w osadzie czynnym bioreaktora membranowego
Autorzy:: Ziembińska-Buczyńska, A.
Wiszniowski, J.
Ciesielski, S.
Powiązania:: https://bibliotekanauki.pl/articles/204755.pdf
Data publikacji:: 2014
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: ammonia oxidizing bacteria
AOB
16S rRNA gene
Polymerase Chain Reaction – Denaturing Gradient Gel Electrophoresis
PCR-DGGE
nested-PCR
utlenianie amoniaku
bakteria utleniająca amoniak
gen kodujący 16S rRNA
Opis:: Nitritation, the first stage of ammonia removal process is known to be limiting for total process performance. Ammonia oxidizing bacteria (AOB) which perform this process are obligatory activated sludge habitants, a mixture consisting of Bacteria, Protozoa and Metazoa used for biological wastewater treatment. Due to this fact they are an interesting bacterial group, from both the technological and ecological point of view. AOB changeability and biodiversity analyses both in wastewater treatment plants and lab-scale reactors are performed on the basis of 16S rRNA gene sequences using PCR-DGGE (Polymerase Chain Reaction – Denaturing Gradient Gel Electrophoresis) as a molecular biology tool. AOB researches are usually led with nested PCR. Because the application of nested PCR is laborious and time consuming, we have attempted to check the possibility of using only first PCR round to obtain DGGE fingerprinting of microbial communities. In this work we are comparing the nested and non-nested PCR-DGGE monitoring of an AOB community and presenting advantages and disadvantages of both methods used. The experiment revealed that PCR technique is a very sensitive tool for the amplification of even a minute amount of DNA sample. But in the case of nested-PCR, the sensitivity is higher and the template amount could be even smaller. The nested PCR-DGGE seems to be a better tool for AOB community monitoring and complexity research in activated sludge, despite shorter fragments of DNA amplification which seems to be a disadvantage in the case of bacteria identification. It is recommended that the sort of analysis approach should be chosen according to the aim of the study: nested-PCR-DGGE for community complexity analysis, while PCR-DGGE for identification of the dominant bacteria.
Nitiritacja – pierwszy etap nitryfikacji, jest uznawany za krok limitujący przebieg całości procesu utleniania amoniaku. Bakterie utleniające amoniak (ang. ammonia oxidizing bacteria, AOB), które prowadzą ten proces są stałymi mieszkańcami osadu czynnego – mieszaniny bakterii, Protozoa i Metazoa, wykorzystywanych do biologicznego oczyszczania ścieków. Z tego powodu są one interesujące zarówno z punktu widzenia technologii, jak i ekologii mikroorganizmów. Analizy zmienności i bioróżnorodności bakterii utleniających amoniak, zarówno w oczyszczalni ścieków, jak i w reaktorach w skali laboratoryjnej, są prowadzone w oparciu o sekwencje genu kodującego 16S rRNA z użyciem metody biologii molekularnej, jaką jest PCR-DGGE (Polymerase Chain Reaction – Denaturing Gradient Gel Electrophoresis). Analizy te są zazwyczaj prowadzone techniką tzw. nested-PCR. Ze względu na fakt, że metoda ta wymaga większego nakładu pracy i czasu, niż tradycyjny jednoetapowy PCR (ang. non-nested PCR) podjęto próbę sprawdzenia możliwości zastosowania techniki jednoetapowego PCR do uzyskania wzorów prążkowych DGGE bakterii utleniających amoniak. W tej pracy zaprezentowano wyniki analizy PCR-DGGE z użyciem technik nested i non-nested PCR oraz podjęto próbę wykazania ich wad i zalet.
Źródło:: Archives of Environmental Protection; 2014, 40, 4; 31-38
2083-4772
2083-4810
Pojawia się w:: Archives of Environmental Protection
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 9.

Tytuł:: NESTED PCR w diagnostyce przypadkow pneumocystozowego zapalenia pluc [PCP] u pacjentow z Aids
Autorzy:: Golab, E
Sobolewska, A.
Bitkowska, E.
Bednarska, A.
Berak, H.
Dzbenski, T.H.
Horban, A.
Powiązania:: https://bibliotekanauki.pl/articles/840596.pdf
Data publikacji:: 2001
Wydawca:: Polskie Towarzystwo Parazytologiczne
Tematy:: zapalenie pluc
metody diagnostyczne
czynniki chorobotworcze
choroby czlowieka
parazytologia lekarska
lancuchowa reakcja polimerazy
pneumocystoza
diagnostyka
AIDS
ludzie chorzy
Pneumocystis carinii
metoda nested-PCR
Opis:: The studies were undertaken to evaluate the usefulness of a nested PCR assay in the diagnosis of Pneumocystis carinii pneumonia (PCP) in AIDS patients. To achieve the end, 51 excretions samples from the respiratory traci were collected from HIV-infected patients with respiratory symptoms, and examined for the presence of specific DNA. A portion of the mitochondrial large-subunit rRNA gene of Pneumocystis carinii was amplified with outer primers pair pAZ 102E, pAZ 102H and internal primers pAZ 102X, pAZ 102Y. Positive nested PCR results were obtained with 36 out of 51 examined samples. Some 52.8% of the positive results were obtained with samples collected from patients without clinical diagnosis of PCP. It was concluded, that the nested PCR method, being too sensitive, is not suitable for the routine diagnosis of PCP in AIDS patients.
Źródło:: Annals of Parasitology; 2001, 47, 3; 521-525
0043-5163
Pojawia się w:: Annals of Parasitology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 10.

Tytuł:: Metody molekularne i immunologiczne stosowane w diagnostyce grzybic
Molecular and immunological methods applied in diagnosis of mycoses
Autorzy:: Kuba, K
Powiązania:: https://bibliotekanauki.pl/articles/841048.pdf
Data publikacji:: 2008
Wydawca:: Polskie Towarzystwo Parazytologiczne
Tematy:: diagnostyka lekarska
diagnostyka molekularna
metody diagnostyczne
diagnostyka immunologiczna
metody molekularne
metoda nested-PCR
testy serologiczne
metoda EIA
metoda RT-PCR
metoda RAPD
markery molekularne
metoda RFLP
lancuchowa reakcja polimerazy
metoda AFLP
metody immunologiczne
grzybice
Źródło:: Annals of Parasitology; 2008, 54, 3; 187-197
0043-5163
Pojawia się w:: Annals of Parasitology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 11.

Tytuł:: Metody molekularne i immunologiczne stosowane w diagnostyce grzybic
Molecular and immunological methods applied in diagnosis of mycoses
Autorzy:: Kuba, K.
Powiązania:: https://bibliotekanauki.pl/articles/2143775.pdf
Data publikacji:: 2008
Wydawca:: Polskie Towarzystwo Parazytologiczne
Tematy:: diagnostyka lekarska
diagnostyka molekularna
metody diagnostyczne
diagnostyka immunologiczna
metody molekularne
metoda nested-PCR
testy serologiczne
metoda EIA
metoda RT-PCR
metoda RAPD
markery molekularne
metoda RFLP
lancuchowa reakcja polimerazy
metoda AFLP
metody immunologiczne
grzybice
Opis:: The diagnosis of fungal infections remains a problem for the management of fungal diseases, particularly in the immunocompromised patients. Systemic Candida infections and invasive aspergillosis can be a serious problem for individuals who need intensive care. Traditional methods used for the identification and typing of medically important fungi, such as morphological and biochemical analysis, are time−consuming. For the diagnosis of mycoses caused by pathogenic fungi faster and more specific methods, especially after the dramatic increase in nosocomial invasive mycoses are needed. New diagnostic tools to detect circulating fungal antigens in biological fluids and PCR−based methods to detect species or genus−specific DNA or RNA have been developed. Antigen detection is limited to searching only one genus. Molecular genetic methods, especially PCR analysis, are becoming increasingly important as a part of diagnostics in the clinical mycology laboratory. Various modifications of the PCR method are used to detect DNA in clinical material, particularly multiple, nested and real−time PCR. Molecular methods may be used to detection of nucleic acids of fungi in clinical samples, to identify fungal cultures at the species level or to evaluate strain heterogeneity differences within the species. This article reviews some of the recent advances in the possibility of molecular diagnosis of fungal infections.
Źródło:: Wiadomości Parazytologiczne; 2008, 54, 3; 187-197
0043-5163
Pojawia się w:: Wiadomości Parazytologiczne
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 12.

Tytuł:: Metody identyfikacji gatunkowej grzybów z rodzaju Candida. Część II. Techniki molekularne
Methods for species identification of genus Candida fungi. Part II. Molecular techniques
Autorzy:: Gnat, Sebastian
Powiązania:: https://bibliotekanauki.pl/articles/22326536.pdf
Data publikacji:: 2022
Wydawca:: Krajowa Izba Lekarsko-Weterynaryjna
Tematy:: choroby grzybicze
grzybice
Candida
identyfikacja gatunkowa
metody
metody molekularne
test PCR
metoda multiplex PCR
metoda nested-PCR
metoda real time PCR
fluorescencyjna hybrydyzacja in situ
spektrometria mas MALDI-TOF MS
czynniki chorobotwórcze
grzyby chorobotwórcze
łańcuchowa reakcja polimerazy
piroliza ze spektometrią mas
identification
molecular techniques
MALDI-TOF MS
Opis:: A rapid and accurate identification of the Candida is crucial for clinical treatment of local and systemic candidiasis. Premature diagnosis of invasive fungal infections is problematic because most clinical signs are non-specific and cultures are often negative or become positive too late for the initiation of effective antifungal therapy. Therefore, studies have been performed to improve molecular techniques for the diagnosis of candidiasis. Today, molecular strategies, such as PCR and non-PCR based methods are used to complement conventional laboratory approach. They provide accurate results in much short time of 1.5–3 h. Given the high accuracy and time saving with molecular typing techniques it is likely that most of these methods could improve routine clinical laboratory identification of Candida yeast species. However, further studies are needed for standardization of such procedures. This article presents an overview and discussion on molecular identification methods in the context of their application for the diagnosis of Candida fungi. Particular attention has been focused on the advantages and limitations of the indicated methods and the possibilities of their implementation for routine use in clinical laboratories.
Źródło:: Życie Weterynaryjne; 2022, 97, 03; 167-174
0137-6810
Pojawia się w:: Życie Weterynaryjne
Dostawca treści:: Biblioteka Nauki