Temat: naked DNA - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Direct in vivo transfer of plasmid DNA into murine tumors: Effects of endotoxin presence and transgene localization.
Autorzy:: Budryk, Magdalena
Cichoń, Tomasz
Szala, Stanisław
Powiązania:: https://bibliotekanauki.pl/articles/1044111.pdf
Data publikacji:: 2001
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: DNA transfer in vivo
endotoxin
myofibroblasts
naked DNA
Opis:: The purpose of this study was to investigate the effect of endotoxin presence in plasmid DNA preparations on the efficiency of transfection achieved in vivo with B16(F10) and Renca tumors and to determine transgene localization. Our data show that endotoxin markedly decreases the efficiency of transfection. Furthermore, the transgene transferred in vivo can be found in both neoplastic and normal (most likely myofibroblast) cells lying in proximity of the administration site.
Źródło:: Acta Biochimica Polonica; 2001, 48, 3; 795-800
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Direct transfer of IL-12 gene into growing Renca tumors.
Autorzy:: Budryk, Magdalena
Wilczyńska, Urszula
Szary, Jarosław
Szala, Stanisław
Powiązania:: https://bibliotekanauki.pl/articles/1044365.pdf
Data publikacji:: 2000
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: IL-12 gene
tumor gene therapy
naked DNA
Opis:: We investigated the feasibility of transferring naked plasmid DNA containing a therapeutic gene (IL-12) into mice harboring growing Renca tumors. We found that naked DNA transferred into growing Renca and B16(F10) tumors gives higher expression level of reporter gene than complexes of DNA with DDAB/ DOPE or DC-Chol/DOPE. Transfer of naked DNA carrying the IL-12 gene into growing Renca tumors causes a distinct therapeutic effect that depends on the time span between inoculation of mice with cancer cells and the beginning of the therapy. Therapy started on day 3 resulted in total cure (100%) of mice.
Źródło:: Acta Biochimica Polonica; 2000, 47, 2; 385-391
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: pVAX1 plasmid vector-mediated gene transfer of soluble TRAIL suppresses human hepatocellular carcinoma growth in nude mice
Autorzy:: Zhang, Yan
Ma, Cun
Liu, Hua
Zhang, Xiu
Sun, Wen
Powiązania:: https://bibliotekanauki.pl/articles/1041078.pdf
Data publikacji:: 2007
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: pVAX1
soluble TRAIL
naked DNA
gene therapy
hepatocellular carcinoma
Opis:: The extracellular domain of the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) may function as a soluble cytokine to selectively kill various cancer cells without toxicity to most normal cells. We used a high-biosafety plasmid pVAX1 as a vector and constructed a recombinant plasmid expressing the extracellular domain (95-281 aa) of human TRAIL fused with signal peptides of human IgGγ, designated as pVAX-sT. Transduction of human BEL7402 liver cancer cells with pVAX-sT led to high levels of sTRAIL protein in the cell culture media and induced apoptosis. The therapeutic potential of pVAX-sT was then evaluated in the BEL7402 transplanted naked mouse model. Subsequent intratumoral administration of naked pVAX-sT resulted in the expression of soluble TRAIL in the sera and the tumor site, as well as effective suppression of tumor growth, with no toxicity to liver. In conclusion, the successful inhibition of liver cancer growth and the absence of detectable toxicity suggest that pVAX-sT could be useful in the gene therapy of liver cancer.
Źródło:: Acta Biochimica Polonica; 2007, 54, 2; 307-313
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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