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    Wyświetlanie 1-9 z 9
    Tytuł:
    Influence of cysteine on mechlorethamine - induced chromosomal aberrations in cultured human lymphocytes
    Autorzy:
    Blaszczyk, A
    Powiązania:
    https://bibliotekanauki.pl/articles/2048200.pdf
    Data publikacji:
    1995
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    cysteine
    lymphocyte
    human lymphocyte
    chromosome aberration
    in vitro
    mechlorethamine
    mutagenicity test
    Opis:
    The aim of the present paper was to find out by in vitro chromosomal aberration test using human lymphocytes whether cysteine has anticlastogenic properties towards a well-known mutagen - mechlorethamine. The lymphocytes tested were obtained from three healthy donors. Two doses of cysteine (1.0 and 2.0 μg/ml) and three doses of mechlorethamine (0.1,0.2 and 0.3 μg m⁻¹) were tested. It was found that cysteine had anticlastogenic properties and that it reduced the number of metaphases with chromosomal aberrations induced by mechlorethamine.
    Źródło:
    Journal of Applied Genetics; 1995, 36, 4; 389-393
    1234-1983
    Pojawia się w:
    Journal of Applied Genetics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Computational predictive mutagenicity of similar chemicals for anthraquinone, β-sitosterol and quercetin found in Alternanthera tenella by using QSAR modeling software
    Autorzy:
    Haque, Mahmudul
    Bhakat, Ram Kumar
    Bhattacharjee, Aloke
    Talapatra, Soumendra Nath
    Powiązania:
    https://bibliotekanauki.pl/articles/1182926.pdf
    Data publikacji:
    2016
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Allelopathy; Allelochemicals; Alternanthera tenella; Invasive species; Terrestrial ecosystem; Predictive Ames mutagenicity; QSAR modeling; T.E.S.T. software
    Opis:
    The present study aims to evaluate the mutagenic potential of secondary metabolites viz. anthraquinone, β-sitosterol and quercetin present in Alternanthera tenella and their related analogus compounds similar in their molecular structure. Nine similar putative allelochemicals analogus to each of anthraquninone, β-sitosterol and quercetin respectively were selected, a total of twenty seven similar chemicals were studied for mutagenicity prediction. Ames mutagenicity prediction was carried out by using T.E.S.T. (Toxicity Estimation Software Tool) of USEPA. All experimental metadata were obtained from Toxicity Benchmark and T.E.S.T. The results clearly indicated that the allelochemicals, anthraquinone and its related eight compounds were mutagenic positive except benzanthrone (mutagenic negative) but all experimental data were found mutagenic positive. β-sitosterol showed mutagenic negative in both experimental and predicted value. It’s three related compounds were mutagenic positive but rest six related compounds mutagenic negative in predicted value while in experimental data, seven compounds were found mutagenic positive and rest two mutagenic negative. In case of quercetin, both data were obtained mutagenic positive while in related compounds, seven compounds were found to be mutagenic positive and two compounds mutagenic negative in predicted value. All were found to be mutagenic positive in experimental metadata. Such findings poses a curiosity that are there any possibilities of conversion or substitution in the position of aromatic ring of allelochemicals when present in soil? Because allelopathy depends upon several environmental stressors and mutagenicity may be induced by allelochemicals. It is suggesting for future research to detect metabolic pathway and mechanism of allelochemicals formation in A. tenella in presence of toxins in soil and to validate with other available 2D and 3D softwares.
    Źródło:
    World Scientific News; 2016, 49, 2; 162-191
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    EVALUATION OF MUTAGENIC PROPERTIES OF 2(C6H15N2O2)+2I3-H2O IODINE COORDINATION COMPLEX IN BACTERIAL REVERSE MUTATION TEST
    Autorzy:
    Jumagaziyeva, Ardak B.
    Iskakbayeva, Zhanar A.
    Myrzabayeva, Auyes N.
    Suldina, Natalya A.
    Paretskaya, Nataliya A.
    Datkhaev, Ubaidulla M.
    Flisyuk, Elena V.
    Ilin, Aleksandr I.
    Powiązania:
    https://bibliotekanauki.pl/articles/895360.pdf
    Data publikacji:
    2020-06-29
    Wydawca:
    Polskie Towarzystwo Farmaceutyczne
    Tematy:
    mutagenicity
    Ames test
    iodine coordination complex
    Opis:
    Objective: 2(C6H15N2O2)+ 2I3-H2O iodine coordination complex is a new pharmaceutical substance that have high antibacterial activity against both sensitive and multidrug resistant strains of Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa. Methods: Mutagenicity of 2(C6H15N2O2)+2I3-H2O iodine coordination complex was assessed using Salmonella/microsome (Ames) test. Tester strains used were Salmonella typhimurium TA98, TA100, TA1535, TA 1537 and Escherichia coli WP2uvrA. The mutagenic activity was determined both in the absence or presence of S9 mixture. Results: The study have shown that test item did not cause any increase in the number of his+ revertants in S.typhimurium and trp+revertants in E.coli WP2uvrA strains in the presence or absence of S9-mix, compared to the controls. Conclusion: Under the conditions of this test and according to the criteria set for the evaluation of the test results, the 2(C6H15N2O2)+ 2I3-H2O iodine coordination complex did not show mutagenic activity in the S. typhimurium and E.coli plate incorporation assay.
    Źródło:
    Acta Poloniae Pharmaceutica - Drug Research; 2020, 77, 3; 465-473
    0001-6837
    2353-5288
    Pojawia się w:
    Acta Poloniae Pharmaceutica - Drug Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Assessment of mutagenic activity of methyl- and phenylphenanthrenes based on Salmonella test and micronucleus test
    Autorzy:
    Rudnicka, K.
    Tejs, S.
    Budzikur, K. A.
    Mielżyńska-Švach, D.
    Jakimiuk, E.
    Chachaj, A.
    Góra, M.
    Żelazna, K.
    Łuczyński, M. K.
    Powiązania:
    https://bibliotekanauki.pl/articles/363178.pdf
    Data publikacji:
    2013
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    Ames test
    in vitro
    micronucleus test
    mutagenicity
    phenanthrene
    test Amesa
    mutagenność
    test mikrojądrowy
    fenanatren
    Opis:
    Polycyclic aromatic hydrocarbons (PAHs) are widely spread environmental pollutants mainly originating from anthropogenic sources such as fossil fuel combustion, industries, and others. Although a large body of literature exists on the toxicity and carcinogenicity of PAHs, primarily benzo[a]pyrene, toxicity data for phenanthrene deriveratives are very limited. The main aim of the experiment was to investigate if there exists correlation between molecular structure and mutagenic activity of four phenanthrene derivatives: 1 methylphenanthrene, 4 methylphenanthrene, 1 phenylphenanthrene, and 4 phenylphenanthrene. An Ames assay using two strains of histidine dependent Salmonella Typhimurium (TA98 and TA100) was conducted to assess the mutagenic activity of studied compounds both in the presence (+S9) and in the absence (-S9) of an exogenous source of metabolic activation. The compounds were also tested in an in vitro chromosome aberration assay in which V-79 cells were exposed to the phenanthrene derivatives investigated both in the presence and in the absence of metabolic activation. The phenylphenanthrenes showed no mutagenic effect. These compounds occasionally induced significant decrease in the number of revertants in the Ames test. The greatest mutagenic effects were observed for 1 methylphenanthrene after metabolic activation (+S9). In the micronucleus test the greatest mutagenic effect was observed for 4 methylphenanthrene also in the presence of metabolic activation system. The results obtained are comparable to those reported earlier for the methylphenanthrenes.
    Źródło:
    Environmental Biotechnology; 2013, 9, 2; 65-71
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    2,4-diphenylthiophene induces mainly base pair mutation in Salmonella Typhimurium
    Autorzy:
    Budzikur, K. A.
    Mielżyńska-Švach, D.
    Góra, M.
    Chachaj, A.
    Pawłowski, M.
    Łuczyński, M. K.
    Powiązania:
    https://bibliotekanauki.pl/articles/363086.pdf
    Data publikacji:
    2012
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    test Amesa
    związki heterocykliczne
    mutagenność
    siarkowe wielopierścieniowe węglowodory aromatyczne
    Ames test
    heterocyclic compounds
    mutagenicity
    sulfur-PAHs
    Opis:
    Heterocyclic aromatic compounds containing sulfur (S-HET), have been detected in air, soil, marine environment and freshwater sediment. Toxicity and mutagenicity data of this class of substances are scarce. The present study focuses on implications of two aryl thiophenes and their mutagenic properties in Salmonella/microsome test. In our experiment only 2,4-diphenylthiophene showed little mutagenic effect in both variants of activaction (+/-S9) in strain TA100. Thiophene ring joined to K-region of phenanthrene did not change the biological activity of 3,6-dimetoxyphenanthro [9,10-c]thiophene and this compound did not show mutagenic potency.
    Źródło:
    Environmental Biotechnology; 2012, 8, 1; 28-31
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The Ames test: a methodological short review
    Autorzy:
    Tejs, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/363234.pdf
    Data publikacji:
    2008
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    Salmonella
    test Amesa
    mutagenność
    krótkoterminowe testy in-vitro
    Ames test
    mutagenicity
    short term in-vitro tests
    Opis:
    The Ames Salmonella/microsome mutagenicity assay (Salmonella test; Ames test) is a short-term bacterial reverse mutation assay specifically designed to detect a wide range of chemical substances that can produce genetic damage that leads to gene mutations. The test is used to evaluate the mutagenic properties of test articles. The Ames test uses amino acid-dependent strains of Salmonella typhimurium and Escherichia coli, each carrying different mutations in various genes in the histidine operon. These mutations act as hot spots for mutagens that cause DNA damage via different mechanisms. In the absence of an external histidine source, cells cannot grow and form colonies. Only those bacteria that revert to histidine independence (his+) are able to form colonies. The number of spontaneously induced revertant colonies per plate is relatively constant. However, when a mutagen is added to the plate, the number of revertant colonies per plate is increased, usually in a dose-related manner. The Ames test is used worldwide as an initial screen to determine the mutagenic potential of new chemicals and drugs. The purpose of this publication is to help researchers who apply the Ames test in their studies.
    Źródło:
    Environmental Biotechnology; 2008, 4, 1; 7-14
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Genotoksyczność antybakteryjnych bioszkieł wytworzonych metodą zol-żel wobec Salmonella typhimurium
    Genotoxicity of antibacterial bioglasses obtained by sol-gel method for Salmonella typhimurium
    Autorzy:
    Jadczyk, P.
    Umińska-Wasiluk, B.
    Ciołek, L.
    Karaś, J.
    Olszyna, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/283769.pdf
    Data publikacji:
    2016
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    bioszkło wapniowokrzemianowe
    bioszkło glinokrzemianowe
    biomateriał
    test rewersji mutacji
    mutagenność
    calciumsilicate bioglass
    aluminosilicate bioglass
    biomaterial
    reverse mutation test
    mutagenicity
    Opis:
    Problemem w stomatologii są choroby przyzębia. W zaawansowanej fazie tej choroby konieczna jest chirurgiczna interwencja z zastosowaniem odpowiednich biomateriałów dla regeneracji tkanek. Dlatego celem tej pracy było określenie genotoksyczności potencjalnych biomateriałów w postaci bioszkła glinokrzemianowego (B-I) oraz bioszkieł wapniowokrzemianowych (Z-5 i Z-8) wobec Salmonella typhimurium TA 98 i TA 100 w mikropłytkowym teście rewersji mutacji. Bioszkła ekstrahowano w 10 cm3 dimetylosulfotlenku (DMSO), wytrząsając przy 250 rpm po 2 g bioszkieł B-I i Z-8 oraz 1 g bioszkła Z-5 przez 72 h w temperaturze 37°C. Ekstrakty bioszkieł wprowadzano do testu w postaci roztworów w DMSO. Rozcieńczano je połowicznie, tak aby w czasie ekspozycji uzyskać dawki bioszkieł B-I i Z-8: 0,25-8,0 mg/cm3 a bioszkła Z-5: 0,125-8,0 mg/cm3. Wykonano testy bez i z aktywacją metaboliczną 30% frakcją S9. Bioszkło B-I powodowało rewersję mutacji w szczepie TA 100 w obecności frakcji S9. Pozwala to wnioskować, że bioszkle B-I występowały mutageny pośrednie powodujące powstawanie mutacji podstawiania par zasad na wykrywanie których pozwala szczep TA100. Dane literaturowe wskazują, że mogło to być następstwem łącznego działania składników tego bioszkła oraz pozostałości substratów użytych do ich wytworzenia. Nie zawierało ono mutagenów bezpośrednich powodujących powstawanie mutacji podstawiania par zasad ani mutagenów bezpośrednich i pośrednich powodujących powstawanie mutacji zmiany fazy odczytu, na wykrywanie których pozwala szczep TA98. Bioszkła Z-5 i Z-8 nie powodowały rewersji mutacji wobec żadnego ze stosowanych szczepów testowych. Uzyskane wyniki pozwalają rekomendować bioszkła Z-5 i Z-8 do dalszych badań poprzedzających ich kliniczne zastosowanie. Bioszkło B-I nie powinno być stosowane w chirurgicznym leczeniu chorób przyzębia.
    Periodontal disease causes problems in dentistry. Surgical intervention with appropriate biomaterials for tissue regeneration is necessary in advanced stages of the disease. Therefore, the aim of this study was to determine the genotoxicity of potential biomaterials in the form of aluminosilicate bioglass (B-I) and calciumsilicate bioglasses (Z-5 and Z-8) for Salmonella typhimurium TA 98 and TA 100 in the microplate reverse mutation test. The bioglasses were extracted with 10 cm3 of dimethyl sulfoxide (DMSO), shaken at 250 rpm for 2 g of B-I and Z-8 bioglass and 1 g of Z-5 bioglass for 72 h at 37°C. Extracts of bioglasses were introduced to the test in the form of solutions in DMSO. They were partially diluted, so that during the exposure 0.25-8.0 mg/cm3 dose of B-I and Z-8 bioglasses and 0.125-8.0 mg/cm3 dose of Z-5 bioglass were obtained. Tests were carried out with and without metabolic activation at 30% of S9 fraction. B-I bioglass caused a reversion of mutations in the TA 100 strain in the presence of S9 fraction. This suggests that indirect mutagens occurred in B-I bioglass that cause base substitution mutations, which TA100 strain detect. Literature data suggests that this could be a consequence of the combined effect of the components of this bioglass and the remains of the substrates used to produce them. Z-5 and Z-8 bioglasses did not result in the reversion of the mutation against any of the test strains used. The results indicate that there should be further study of Z-5 and Z-8 bioglasses prior to their clinical application, and that B-I bioglass should not be used in the surgical treatment of periodontal disease.
    Źródło:
    Engineering of Biomaterials; 2016, 19, 135; 21-27
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    QSAR modeling for prediction of acute toxicity and mutagenicity in different test models by established common phytochemicals present in Phyllanthus niruri
    Autorzy:
    Dhar, Shrinjana
    Gupta, Kaushik
    Talapatra, Soumendra Nath
    Powiązania:
    https://bibliotekanauki.pl/articles/1192089.pdf
    Data publikacji:
    2016
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    QSAR modeling
    Common phytochemicals
    Phyllanthus niruri
    T.E.S.T. software
    Predictive toxicity and mutagenicity
    Opis:
    In globe, Pyllanthus niruri is a well-established medicinal herb studied by many researchers, grown widely in many parts of West Bengal. The present study was aimed to predict the acute toxicity as LC50 in Daphnia magna and Pimephales promelas and rat oral LD50 value as well as Ames mutagenicity by using QSAR modeling software, T.E.S.T. (Toxicity Estimation Software Tool) for commonly found phytochemicals in Pyllanthus niruri. In present works, the data were obtained for LC50, few phytochemicals were toxic to D. magna and P. promelas and also mutagenic but rat oral LD50 determined less toxic. The present QSAR modeling work is suggesting that more researches should be required through experimental as well as predictive study with other prescribed software to know the mechanisms of toxicity and mutagenicity for these combined form of phytochemicals after separating each natural chemical from extract prior to drugs development for therapeutic usage.
    Źródło:
    World Scientific News; 2016, 37; 202-219
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Wykrywanie obecnosci zwiazkow rakotworczych w srodowisku ze szczegolnym uwzglednieniem testow na muszce owocowej [Drosophila melanogaster]
    Autorzy:
    Krogulski, A
    Powiązania:
    https://bibliotekanauki.pl/articles/877652.pdf
    Data publikacji:
    1992
    Wydawca:
    Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny
    Tematy:
    Drosophila melanogaster
    nowotwory
    choroby czlowieka
    muszka owocowa
    mutagennosc
    testy immunologiczne
    genetyka zwierzat
    substancje rakotworcze
    cancer
    human disease
    fruit fly
    mutagenicity
    immunological test
    animal genetics
    carcinogenic substance
    Opis:
    Praca zawiera krótki przegląd metod wykrywania związków rakotwórczych w środowisku człowieka przy podkreśleniu zalet stosowania testu mutacji i rekombinacji u muszki owocowej - Drosophila melanogaster.
    The increasing exposure of the human population to mutagen and carcinogenic substances necessitates research and introduction of methods for their detection. The diersity of these dangerous substances in the environment requires, the application of the chemical and biological tests. The latter allow testing of chemical mixtures of unknown composition. The advantages and limitations of short-term tests are presented. Among these the advantages of somatic mutation and recombination tests are described in more detail on Drosophila melanogaster. The subcellular structure, enzymes produced and the course of metabolic processes in Drosophila are similar as in man. It is possible, therefore, to detect the numerous mutagens undetectable in tests on bacteria. These test are relaitvely simple, not expensive and do not require expensive apparatus or preliminary activation of promuta- gens necessary in tests on bacteria.
    Źródło:
    Roczniki Państwowego Zakładu Higieny; 1992, 43, 3-4; 271-275
    0035-7715
    Pojawia się w:
    Roczniki Państwowego Zakładu Higieny
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-9 z 9

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