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Tytuł:
Mitochondrial DNA in pediatric leukemia patients
Autorzy:
Kodroń, Agata
Ghanim, Magda
Krawczyk, Katarzyna
Stelmaszczyk-Emmel, Anna
Tońska, Katarzyna
Demkow, Urszula
Bartnik, Ewa
Powiązania:
https://bibliotekanauki.pl/articles/1038705.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
pediatric ALL
mtDNA
chemotherapy
Opis:
Numerous studies of mitochondrial DNA (mtDNA) in cancer have shown differences between mtDNA sequences in tumor and normal tissue and at various stages of cancer treatment in the same patient. However, there is little data on acute lymphoblastic leukemia (ALL), the most common type of leukemia in children. In this study we compared mitochondrial sequence variation in the D-loop region and in 5 genes of mtDNA in bone marrow samples of 6 pediatric patients with ALL at various stages of therapy. We found several common polymorphisms and one variant at position 3688 whose level varied during leukemia treatment. Our results suggest that mitochondrial DNA mutations, whose levels change during patient treatment, could be potential biomarkers for monitoring treatment efficacy and disease progression.
Źródło:
Acta Biochimica Polonica; 2017, 64, 1; 183-187
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Role of mitochondria in carcinogenesis
Autorzy:
Tokarz, Paulina
Blasiak, Janusz
Powiązania:
https://bibliotekanauki.pl/articles/1039194.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
cancer
mitochondria
mtDNA
ROS
anti-cancer therapy
Opis:
Mitochondria play the central role in supplying cells with ATP and are also the major source of reactive oxygen species (ROS) - molecules of both regulatory and destructive nature. Dysfunction of mitochondrial metabolism and/or morphology have been frequently reported in human cancers. This dysfunction can be associated with mitochondrial DNA (mtDNA) damage, which may be changed into mutations in mtDNA coding sequences, or the displacement-loop region, changes in the mtDNA copy number or mtDNA microsatellite instability. All these features are frequently associated with human cancers. Mutations in mtDNA can disturb the functioning of the ROS-producing organelle and further affect the entire cell which may contribute to genomic instability typical for cancer cells. Although the association between some mtDNA mutations and cancer is well established, the causative relationship between these two features is largely unknown. A hint suggesting the driving role of mtDNA mutations in carcinogenesis comes from the observation of tumor promotion after mtDNA depletion. Mitochondria with damaged DNA may alter signaling of the mitochondrial apoptosis pathway promoting cancer cell survival and conferring resistance to anticancer drugs. This resistance may be underlined by mtDNA copy number depletion. Therefore, mitochondria are considered a promising target in anticancer therapy and several mitochondria-targeting drugs are in preclinical and clinical trials. Some other aspects of mitochondrial structure and functions, including morphology and redox potential, can also be associated with cancer transformation and constitute new anticancer targets. Recently, several studies have disclosed new mechanisms underlying the association between mitochondria and cancer, including the protection of mtDNA by telomerase, suggesting new approaches in mitochondria-oriented anti-cancer therapy.
Źródło:
Acta Biochimica Polonica; 2014, 61, 4; 671-678
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Characterization of conditions and determination of practical tips for mtDNA level estimation in various human cells
Autorzy:
Jędrak, Paulina
Sowa, Natalia
Barańska, Sylwia
Węgrzyn, Grzegorz
Powiązania:
https://bibliotekanauki.pl/articles/1038562.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
mtDNA level
leukocytes
fibroblasts
qPCR methodology
Huntington disease
Opis:
Determination of mtDNA copy number in the cell is crucial to understand many cellular processes. Recently, the number of studies with the use of mitochondrial DNA (mtDNA) content as the determinant of mitochondrial abnormalities increased greatly and is still growing, therefore, optimization of technical conditions for this analysis is crucial. Despite using similar laboratory protocols, some results cannot be compared between research centers, thus causing discrepancies in the assessment of mtDNA content. The aim of this work was to test which conditions of biological sample collection and storage affect estimation of mtDNA level relative to the nuclear DNA (nDNA) in the blood samples and dermal fibroblasts. We found that the time and temperature of sample storage, as well as the type of the blood sample (whole blood or leukocytes) influence the estimate of mtDNA/nDNA ratio in the blood. In the case of dermal fibroblasts collected from healthy control and Huntington disease patients, our data indicate that the passage number of cells is essential to obtain reliable results.
Źródło:
Acta Biochimica Polonica; 2017, 64, 4; 699-704
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparison between the Polish population and European populations on the basis of mitochondrial morphs and haplogroups.
Autorzy:
Piechota, Janusz
Tońska, Katarzyna
Nowak, Magda
Kabzińska, Dagmara
Lorenc, Anna
Bartnik, Ewa
Powiązania:
https://bibliotekanauki.pl/articles/1041498.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
homoplasy
Polish population
haplogroup
morph
haplotype
mtDNA evolution
Opis:
Polymorphisms in mitochondrial DNA (mtDNA) were analyzed in 152 samples from the Polish population using restriction enzymes AvaI, BamHI, HaeII, HpaI and PstI. Additionally, each sample was classified into the appropriate haplogroup. When required, appropriate fragments were sequenced to establish the exact polymorphic sites. We found one new morph for PstI and six new morphs for AvaII. Some detected morphs have previously been described as population specific morphs in different regions of the world. All polymorphisms were classified into 31 different haplotypes. 21 of them were detected in single individuals. The Polish population was compared with other populations from different regions. Moreover, we have obtained evidence for mutation hot spots in the mtDNA coding region. Our results indicate that AvaII morph and haplogroup composition of the Polish population is similar to other European populations and has a distribution typical for this part of the world. However, statistically significant differences in haplogroup composition were found between the Polish population and Italian and Finnish populations.
Źródło:
Acta Biochimica Polonica; 2004, 51, 4; 883-895
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Nuclear and mitochondrial genome responses in HeLa cells treated with inhibitors of mitochondrial DNA expression
Autorzy:
Piechota, Janusz
Szczęsny, Roman
Wolanin, Kamila
Chlebowski, Aleksander
Bartnik, Ewa
Powiązania:
https://bibliotekanauki.pl/articles/1041204.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
thiamphenicol
mtDNA depletion
HeLa
dideoxycytidine
mitochondrial biogenesis
ethidium bromide
Opis:
The influence of mutations in the mitochondrial DNA (mtDNA) on the bioenergetic metabolism of the cell is still poorly understood. Many of the mutations in the mtDNA affect the expression of the mitochondrial genome. Investigations on cells from patients are not easy, especially as the mitochondrial DNA is heteroplasmic and this state is changed in culture. Moreover, the nuclear background and the mitochondrial haplotype may affect the behaviour of cells. Transfer of patient mitochondria to rho zero cell lines is also not optimal as these cells in general have many nuclear changes which may also affect cell behaviour. Thus, we decided to use inhibitors of mitochondrial genome expression, such as thiamphenicol, ethidium bromide and dideoxycytidine to investigate the bioenergetic metabolism of HeLa cells. We found that oxidative phosphorylation and glycolysis participate equally in ATP production in HeLa cells and that decreased activity of the respiratory chain leads to increased glycolysis and the reduction of cell growth. Insufficient ATP production in the oxidative phosphorylation process was not compensated by increased proliferation of the mitochondria. However, we were able to show that there are some mechanisms compensating limited expression of the mitochondrial genome within the mitochondria. Experiments with dideoxycytidine revealed that 10-fold decrease of the mtDNA copy number resulted in almost normal activity of cytochrome c oxidase. We found that mtDNA depletion is compensated mostly on the level of RNA metabolism in the mitochondria. Thus, our results are in agreement with the hypothesis that transcription initiation rather than mtDNA copy number is a rate limiting factor for expression of the mitochondrial genome.
Źródło:
Acta Biochimica Polonica; 2006, 53, 3; 485-495
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genetic diversity of native Japanese dog breeds in the primitive type
Różnorodność genetyczna rodzimych ras psów japońskich w typie pierwotnym
Autorzy:
Kloch, M.
Życzyński, A.
Nowak-Życzyńska, Z.
Powiązania:
https://bibliotekanauki.pl/articles/2082389.pdf
Data publikacji:
2021
Wydawca:
Szkoła Główna Gospodarstwa Wiejskiego w Warszawie. Wydawnictwo Szkoły Głównej Gospodarstwa Wiejskiego w Warszawie
Tematy:
Canis lupus familiaris
mtDNA
microsatellites
control region
primitive breeds
Opis:
Genetic diversity of native Japanese dog breeds in the primitive type. In the following paper the genetic distance among selected Japanese dog breeds (Akita, Kishu, Kai, Shiba, Shikoku, Hokkaido) was estimated. In order to determine genetic differentiation we analyzed a fragment of the mitochondrial control region and 10 microsatellite loci. We found that variation of the nuclear DNA was larger than that at the level of mitochondrial DNA. Within a fragment of 614 bp of control region, 13 haplotypes were identifi ed. The highest diversity of mitochondrial DNA was found in Akita. Based on mitochondrial DNA analysis, genetic distance between breeds ranged from 0.003 to 0.018. The lowest genetic distance was observed between Shikoku and Kai (0.003), and the highest between Shiba and Akita (0.018). After the analysis of nuclear DNA, 65 alleles were identifi ed. The mean percentage of polymorphic alleles in the applied microsatellite markers was 94.4% ±2.5%. The analysis showed that, the highest distance estimated on the frequency of microsatellite markers was found between Kai and Hokkaido (1.066), and the lowest between Shiba and Kai (0.212). Hokkaido and Shikoku were the only clearly distinguishing breeds.
Źródło:
Annals of Warsaw University of Life Sciences - SGGW. Animal Science; 2019, 58[1]; 37-45
1898-8830
Pojawia się w:
Annals of Warsaw University of Life Sciences - SGGW. Animal Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Analysis of ancient mitochondrial DNA of the Baltic Sea sturgeon (Acipenser sp.)
Autorzy:
Fopp-Bayat, D.
Ciesielski, S.
Luczyński, M.
Powiązania:
https://bibliotekanauki.pl/articles/363174.pdf
Data publikacji:
2005
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
jesiotr
historyczny mtDNA
filogeneza molekularna
gen cytochromu b
Morze Bałtyckie
Acipenser baeri
Acipenser oxyrinchus
Acipenser sturio
ancient mtDNA
cytochrome b gene
molecular phylogeny
Opis:
Genetic relatedness between Baltic Sea sturgeon (Acipenser sturio L.) specimens caught in different geographic areas is not clear. According to previous studies, fish captured in different locations within the historic area of A. sturio habitation are genetically different to each other. We have examined a fragment (191 base pairs) of mitochondrial cytochrome b gene of four specimens of A. sturio found in Poland: three fish were preserved in museums of natural history and the bone of one fish was from an archaeological site. DNA sequences of the three museum samples were identical, whereas the DNA sequence of the archaeological sample differed in the 918 position of the cytochrome b gene. All the analyzed DNA fragments were similar to those of Acipenser baeri and genetically distant to Acipenser sturio and A. oxyrinchus.
Źródło:
Environmental Biotechnology; 2005, 1, 1; 29-33
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Mitochondrial DNA variability in Gyimesi Racka and Turcana sheep breeds
Autorzy:
Kusza, Szilvia
Zakar, Erika
Budai, Csilla
Cziszter, Ludovic-Toma
Padeanu, Ioan
Gavojdian, Dinu
Powiązania:
https://bibliotekanauki.pl/articles/1039104.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
farm animal conservation
genetic diversity
mtDNA
trans-boundary breeds
Zackel group
Opis:
The current knowledge and documentation on the origins and relationship between Gyimesi Racka reared in Hungary and the Romanian Turcana is rather controversial. Lack of information and scientific reliable proofs for the divergent theories found in the two countries motivated us to implement a trial using molecular methods to assess the genetic distance and diversity in the two breeds. Hair follicles were collected from Gyimesi Racka (2 phenotypes) and from Turcana (6 ecotypes). The 599 bp segment of the D-loop region of the mitochondrial DNA was sequenced. Altogether, 42 haplotypes were identified, while 23 were found in both populations. Populations were highly diverse according to the haplotype and nucleotide diversity indices. AMOVA analysis showed that most of the variation was observed within populations (98%), indicating a weak genetic structure between the two breeds. Animals were grouped into seven groups based on their phenotype; however genetic distances among them were also low. Tajima's D, Fu's Fs, goodness-of-fit statistics, mismatch distribution and network analysis suggested recent demographic expansion. Current comprehensive mtDNA study indicates that there is very low level of genetic differentiation between the Gyimesi Racka and Turcana populations therefore they are de facto one trans-boundary breed.
Źródło:
Acta Biochimica Polonica; 2015, 62, 2; 273-280
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Changes in accumulation of heteroplasmic mitochondrial DNA and frequency of recombination via short repeats during plant lifetime in Phaseolus vulgaris
Autorzy:
Woloszynska, Magdalena
Gola, Edyta
Piechota, Janusz
Powiązania:
https://bibliotekanauki.pl/articles/1039695.pdf
Data publikacji:
2012
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Phaseolus vulgaris
sublimons
heteroplasmy
quantitative real-time PCR
plant mitochondrial genome
mtDNA recombination
Opis:
Recombination via short repeats in plant mitochondrial genomes results in sublimons - DNA molecules with a copy number much lower compared to the main mitochondrial genome. Coexistence of stoichiometrically different mitotypes, called heteroplasmy, plays an important evolutionary role, since sublimons occasionally replace the main genome resulting in a new plant phenotype. It is not clear, how frequency of recombination and sublimon production is regulated and how it is related to changes in the quantity of the main genome and sublimons. We analyzed the accumulation of two recombining main genome sequences and two resulting sublimons in apical meristems, undifferentiated tissues and leaves of different age of Phaseolus vulgaris. Copy numbers of the main genome sequences varied greatly depending on tissue type and organ age while accumulation of sublimons remained much more stable. Although the overall accumulation of plant mtDNA decreased with the leaf age, the quantity of sublimons increased relative to the main genome indicating a higher frequency of recombination via the short 314 bp repeat. Recombination was symmetrical in young developing leaves while in senescent tissues it shifted towards asymmetric events resulting in overrepresentation of one product. We propose that during plant lifetime replication and recombination frequencies change oppositely sustaining heteroplasmic compositions of the genome, which are favorable for inheritance and maintenance of complex plant mtDNA.
Źródło:
Acta Biochimica Polonica; 2012, 59, 4; 703-709
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Deroceras panormitanum and congeners from Malta and Sicily, with a redescription of the widespread pest slug as Deroceras invadens n. sp.
Autorzy:
Reise, H.
Hutchinson, J.M.C.
Schunack, S.
Schlitt, B.
Powiązania:
https://bibliotekanauki.pl/articles/83241.pdf
Data publikacji:
2011
Wydawca:
Uniwersytet Mikołaja Kopernika. Wydział Biologii i Ochrony Środowiska. Stowarzyszenie Malakologów Polskich
Tematy:
Deroceras panormitanum
Malta
Sicily
pest
slug
Deroceras invadens
new species
terrestrial slug
taxonomy
anatomy
simultaneous hermaphrodite
unilateral copulation
genital morphology
mating behaviour
mtDNA
cryptic species
reproductive isolation
Agriolimacidae
Mediterranean region
COI mtDNA sequencing
Opis:
The name Deroceras panormitanum is generally applied to a terrestrial slug that has spread worldwide and can be a pest; earlier this tramp species had been called Deroceras caruanae. Neither name is appropriate. The taxonomic descriptions apply to a species from Sicily and Malta. This true D. panormitanum and the tramp species are distinct in morphology and mating behaviour. For instance, the penial caecum of D. panormitanum is more pointed, everting faster at copulation. The size of the penial lobe varies considerably In preserved specimens but is always prominent at copulation. D. panormitanum is distinct from the Maltese endemic Deroceras golcheri, but a phylogeny based on mtDNA COI sequences implies that they are more closely related than is the tramp species. D. golcheri has a still closer counterpart on Sicily, but we leave the taxonomy of this “species X” unresolved. In interspecific crosses, D. panormitanum may transfer sperm to the partner’s sarcobelum whereas the partner fails to evert its penis (D. golcheri) or to transfer sperm (the tramp species). Names previously applied to the tramp species originally referred to D. panormitanum or are otherwise invalid, so it is here formally redescribed as D. invadens. Deroceras giustianum Wiktor, 1998 is synonymised with D. panormitanum.
Źródło:
Folia Malacologica; 2011, 19, 4
1506-7629
Pojawia się w:
Folia Malacologica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular characterization of nuclear and cytoplasmic genomes of Solanum × michoacanum (+) S. tuberosum somatic hybrids
Autorzy:
Smyda-Dajmund, P.
Sliwka, J.
Jakuczun, H.
Wasilewicz-Flis, I.
Zimnoch-Guzowska, E.
Powiązania:
https://bibliotekanauki.pl/articles/951216.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
molecular characteristics
nuclear genome
Solanum tuberosum
somatic hybrid
DArT marker
Solanum × michoacanum
mtDNA
cpDNA
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A novel polymorphic mtDNA marker for population studies of the pink shrimp, Farfantepenaeus duorarum (Crustacea, Penaeidae)
Autorzy:
Grabowski, M.
Grater, W.D.
Stuck, K.C.
Powiązania:
https://bibliotekanauki.pl/articles/47488.pdf
Data publikacji:
2004
Wydawca:
Polska Akademia Nauk. Instytut Oceanologii PAN
Tematy:
pink shrimp
Atlantic coast
Penaeidae
mtDNA marker
population structure
Farfantepenaeus duorarum
Mexico Gulf
Crustacea
genetic marker
Opis:
The pink shrimp, Farfantepenaeus duorarum, is one of the most important shrimp species commercially harvested along the Gulf and Atlantic coasts of the US. In this study we developed a mitochondrial marker suitable for population studies of the species. A 611–617 bp hypervariable portion of the AT-rich region of the mt genome was amplified and sequenced. The 617 bp long consensus sequence contained 15 polymorphic insertion/deletion sites and 165 polymorphic substitution sites. Kimura 2-parameter distances ranged from 0.00 to 0.06 with a mean of 0.02. Among the 104 sequences, 100 haplotypes were counted if all mutations were included. If transitions were omitted, 34 haplotypes were observed. The results indicate that the hypervariable portion of the AT-rich region may be an effective marker for revealing the genetic structure of the pink shrimp off the southeast US.
Źródło:
Oceanologia; 2004, 46, 1
0078-3234
Pojawia się w:
Oceanologia
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Evaluation of the suitability of mitochondrial DNA for species identification of microtraces and forensic traces
Autorzy:
Natonek-Wiśniewska, Małgorzata
Krzyścin, Piotr
Powiązania:
https://bibliotekanauki.pl/articles/1038564.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
biological traces
forensic DNA analysis
species identification of forensic DNA
species identification of biological traces
mtDNA
Opis:
The objective of the study was to demonstrate how mitochondrial DNA (mtDNA) can be used to determine the species origin of animal microtraces. The study included pieces of cat and dog hair without the root, a fragment of cooked chicken bone (0.1g), three goose down samples (0.028 g), a pork swab, a pork scratching (5×5×5 mm), and pork lard (0.22 g). DNA was isolated from all of these samples using the method appropriate for the particular source material. The extracts had DNA concentration exceeding 5.4 ng/µl with A260/280 purity range of 1.14-1.88. Next, the samples were subjected to PCR and real-time PCR with species-specific primers and primers complementary to mitochondrial DNA (mtDNA). Control reactions based on the amplification of eukaryotic-specific fragment (18S rRNA) were additionally performed. PCR and real-time PCR products for detection of species-specific mtDNA were obtained for all templates, whereas during the detection of eukaryote DNA no product was obtained for dog and cat hair only. The poor quality of the obtained DNA did not prevent the analysis. The results showed that mitochondrial DNA is suitable for identification of small or highly processed samples, in which genomic DNA often cannot be analyzed.
Źródło:
Acta Biochimica Polonica; 2017, 64, 4; 705-708
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The genus Daphniola Radoman, 1973 (Caenogastropoda: Hydrobiidae) in the Peloponnese, Greece
Autorzy:
Falniowski, A.
Szarowska, M.
Powiązania:
https://bibliotekanauki.pl/articles/83994.pdf
Data publikacji:
2011
Wydawca:
Uniwersytet Mikołaja Kopernika. Wydział Biologii i Ochrony Środowiska. Stowarzyszenie Malakologów Polskich
Tematy:
Daphniola
Caenogastropoda
Hydrobiidae
Peloponnese
Greece
valvatiform
gastropod
mtDNA
anatomy
hydrobiid
protoconch
phylogenetic analysis
Daphniola hadei
reproductive organ
Opis:
A valvatiform hydrobiid gastropod, found in a spring at Dhiaselo, W of Sparta, N. Taigetos Mts., Peloponnese, Greece, was identified as Horatia hadei Gittenberger, 1982. Its protoconch sculpture, female reproductive organs and penis morphology are characteristic of Daphniola Radoman, 1973. Maximum likelihood phylogenetic analysis based on COI (cytochrome oxidase subunit I) fragments of mtDNA proved that the species is congeneric with D. exigua (A. Schmidt, 1856) and D. louisi Falniowski et Szarowska, 2000, and thus belongs to the genus Daphniola, and that D. hadei, D. exigua and D. louisi are species-level distinct taxa.
Źródło:
Folia Malacologica; 2011, 19, 3
1506-7629
Pojawia się w:
Folia Malacologica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zastosowanie metod molekularnych w identyfikacji gatunku, wieku i płci owadów użytecznych w entomologii sądowej
Use of molecular methods in the identification of the species, age and sex of insects useful in forensic entomology
Autorzy:
Stojak, Joanna
Powiązania:
https://bibliotekanauki.pl/articles/1373968.pdf
Data publikacji:
2014
Wydawca:
Centralne Laboratorium Kryminalistyczne Policji
Tematy:
entomologia sądowa
barkoding DNA
mtDNA
metody molekularne
identyfikacja gatunkowa
forensic entomology
DNA barcoding
molecular methods
identification of species
Opis:
Entomologia sądowa wykorzystuje owady do ustalania czasu i przyczyny śmierci, a nawet miejsca, w którym nastąpiła. W tym celu stosowane są dwie metody. Metoda rozwojowa opiera się na wzorcach rozwoju larw w określonych warunkach temperaturowo-środowiskowych. Metoda sukcesyjna analizuje występujące w różnych środowiskach wzorce pojawiania się poszczególnych taksonów na zwłokach. W obu tych metodach najistotniejszą kwestią jest poprawna identyfikacja gatunków. W poniższym artykule zaprezentowane zostały molekularne metody identyfikacji, takie jak barkoding DNA czy analiza krzywych denaturacji DNA o wysokiej rozdzielczości (DNA-HRM-PCR).
Forensic entomology uses insects to determine the time, cause and place of death. To this end, two entomological methods are used. The development-based method uses the patterns of insect larvae development under the specific thermal and environmental conditions. The succession-based method analyzes the sequence of insect succession on the body in various environmental conditions. The proper insect species identification is essential in both methods. In this article, the molecular methods of species, age and sex identification are presented such as DNA barcoding or DNA-HRM-PCR.
Źródło:
Problemy Kryminalistyki; 2014, 286; 22-26
0552-2153
Pojawia się w:
Problemy Kryminalistyki
Dostawca treści:
Biblioteka Nauki
Artykuł
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