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    Wyświetlanie 1-3 z 3
    Tytuł:
    Morphology and developmental potential of bovine parthenotes after spontaneous activation in vitro
    Autorzy:
    Lechniak, D
    Cieslak, D.
    Sosnowski, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2048294.pdf
    Data publikacji:
    1998
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    blastomere
    bovine oocyte
    cytogenetic analysis
    parthenogenetic activation
    morphology
    in vitro
    fertilization system
    blastocyst
    oocyte
    insemination
    morphological evaluation
    maturation
    embryo production
    Opis:
    Spontaneous parthenogenetic activation of bovine oocytes in an in vitro maturation and fertilization system (IVM/IVF) is described. Altogether, 1403 follicular oocytes, collected by the aspiration method, were matured in vitro and then cultured without insemination in the same conditions as a group of inseminated oocytes. After 48-72 h of additional culture, 141 oocytes (10%) were found to be spontaneously activated. Morphological evaluation revealed that the number of blastomeres within parthenotes ranged from 2 to 16 cells, with a minority (15.7%) comprising of 9-16 blastomeres. According to a cytogenetic analysis, only 1.2% of the analysed parthenotes consisted of more than 9 cells. Parthenotes may not be distinguished from embryos produced in vitro and spontaneous parthenogenetic activation in an IVM/IVF system indicates suboptimal culture conditions. A group of non-inseminated oocytes should be included in each experiment to serve as a control. Spontaneously activated bovine parthenotes only occasionaly developed beyond the 8-blastomere stage in a common IVM/IVF system. The incidence of parthenotes interferes with the efficiency of in vitro embryo production but it is doubtful whether it lowers the pregnancy rate after transfer of IVF embryos.
    Źródło:
    Journal of Applied Genetics; 1998, 39, 2; 193-198
    1234-1983
    Pojawia się w:
    Journal of Applied Genetics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A practitioner’s view : a survey and comparison of lemmatization and morphological tagging in German and Latin
    Autorzy:
    Gleim, Rüdiger
    Eger, Steffen
    Mehler, Alexander
    Uslu, Tolga
    Hemati, Wahed
    Lücking, Andy
    Henlein, Alexander
    Kahlsdorf, Sven
    Hoenen, Armin
    Powiązania:
    https://bibliotekanauki.pl/articles/103805.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Instytut Podstaw Informatyki PAN
    Tematy:
    morphological tagging
    lemmatization
    morphologically rich languages
    NLP evaluation modeling
    Opis:
    The challenge of POS tagging and lemmatization in morphologically rich languages is examined by comparing German and Latin. We start by defining an NLP evaluation roadmap to model the combination of tools and resources guiding our experiments. We focus on what a practitioner can expect when using state-of-the-art solutions. These solutions are then compared with old(er) methods and implementations for coarse-grained POS tagging, as well as fine-grained (morphological) POS tagging (e.g. case, number, mood). We examine to what degree recent advances in tagger development have improved accuracy – and at what cost, in terms of training and processing time. We also conduct in-domain vs. out-of-domain evaluation. Out-of-domain evaluation is particularly pertinent because the distribution of data to be tagged will typically differ from the distribution of data used to train the tagger. Pipeline tagging is then compared with a tagging approach that acknowledges dependencies between inflectional categories. Finally, we evaluate three lemmatization techniques.
    Źródło:
    Journal of Language Modelling; 2019, 7, 1; 1-52
    2299-856X
    2299-8470
    Pojawia się w:
    Journal of Language Modelling
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Cytological and genetic evaluation of anther culture-derived doubled haploids in barley
    Autorzy:
    Szarejko, I
    Falk, D E
    Janusz, A
    Nabialkowska, D
    Powiązania:
    https://bibliotekanauki.pl/articles/2046612.pdf
    Data publikacji:
    1997
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    cytological analysis
    doubled haploid
    morphological variation
    genotype
    barley
    genetic evaluation
    gametoclonal variation
    chlorophyll mutation test
    Hordeum vulgare
    anther culture
    Opis:
    Gametoclonal variation among anther culture-derived plants of three barley genotypes was estimated on the basis of cytological analysis (DH1, DH2 generation), observation of morphological variants (DH2, DH3) and chlorophyll mutation test (DH2, DH3). Individual head rows were grown in the field to detect possible chimeric structure of regenerants and to assess the number of variants and mutations in each line. Spontaneously doubled plants were the most frequent class (70%) among regenerants and almost 90% of them were completely fertile. There was a difference in proportion of haploids produced by different genotypes, but the highest frequency observed did not exceed 21%. The remaining regenerants were tetraploid, and contained chromosomal mutations or chimeras. In total, there were about 15% of polyploids and plants carrying chromosomal aberrations (translocations, inversions) among DH1 individuals. The changes in chromosome number and structure were the main source of observed variation. The level of gene mutation induced in vitro was relatively low. No more than 1% of microspore-derived plants expressed visible morphological changes in DH2 progeny. Only two morphological variants derived from the Bruce cultivar proved to be homozygous mutants (dwarf type) stable up the to third generation. The frequency of DH plants carrying chlorophyll mutation was 5.8%, but most of them (82%) were chimeric and had only a small mutation sector. The level of gametoclonal variation depended on the donor plant genotype. The highest proportion of variants and mutations was observed among DH plants derived from the Bruce cultivar, while the lowest was recorded among plants regenerated from anther culture of the doubled haploid line H930-36. Mechanisms leading to the observed variation and implications resulting from the presented experiments concerning implementation of anther culture in barley breeding were discussed. It was concluded that this method resulted in a high frequency of spontaneous doubling, a low frequency of genetic changes, and being less time and effort-consuming than the ’Bulbosum’ technique, can be applied to most barley breeding programs.
    Źródło:
    Journal of Applied Genetics; 1997, 38, 4; 437-452
    1234-1983
    Pojawia się w:
    Journal of Applied Genetics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-3 z 3

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