Temat: microfluidic system - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Precipitation of fluorescent micro- and nano- crystals in polysaccharide shell
Precypitacja mikro- i nanokryształów fluorescencyjnych w otoczce polisacharydowej
Autorzy:: Wasiak, I.
Fery-Forgues, S
Ciach, T.
Powiązania:: https://bibliotekanauki.pl/articles/2072987.pdf
Data publikacji:: 2014
Wydawca:: Stowarzyszenie Inżynierów i Techników Mechaników Polskich
Tematy:: mikrokryształy organiczne
reprecypitacja
mikroukład
organic microcrystals
reprecipitation
microfluidic system
Opis:: Development of organic micro- and nanocrystals is rapidly expanding field due to their potential use in new technology devices in the field of chemistry and life science. Two methods of organic crystals preparation for future modification with polysaccharides used for cancer detection are compared in the paper. Both reprecipitation methods allow one to obtain microcrystals with a similar size range. However, the use of microfluidic system and polysaccharides solution reduces aggregates formation and leads to obtain narrow size distribution crystals.
Rozwój metod otrzymywana organicznych mikro-i nanokryształów wynika z ich potencjalnego zastosowania w obszarze nowych technologii, chemii i nauk przyrodniczych. W pracy porównano dwie metody otrzymywania kryształów dla przyszłych modyfikacji polisacharydami w celu wykrywania nowotworów. Obie proponowane metody umożliwiają uzyskanie mikrokryształów o zbliżonym rozkładzie wielkości. Zastosowanie mikroukładu i roztworu polisacharydu zmniejsza liczbę powstających agregatów i prowadzi do uzyskania kryształów o wąskim rozkładzie średnic.
Źródło:: Inżynieria i Aparatura Chemiczna; 2014, 4; 316--317
0368-0827
Pojawia się w:: Inżynieria i Aparatura Chemiczna
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: New microfluidic device for lactate dehydrogenase (LDH) activity analysis
Autorzy:: Jędrych, E.
Mazur, M.
Grabowska-Jadach, I.
Brzózka, Z.
Powiązania:: https://bibliotekanauki.pl/articles/115778.pdf
Data publikacji:: 2012
Wydawca:: Fundacja na Rzecz Młodych Naukowców
Tematy:: lactate dehydrogenase (LDH) activity
microfluidic system
PDMS
adherent cell culture
cytotoxicity analysis
Opis:: In this paper, we present cytotoxicity analysis (determination of lactate dehydrogenase — LDH activity performed in a designed and fabricated microfl uidic system. This method allowed for analysis of a supernatant collected from A549 (human lung cancer) and HT-29 (human colon cancer epithelial) cells, which were incubated for 24 h with selected compounds. LDH is an intracellular enzyme present in tissues, which is released into the supernatant caused by membrane damage or cell lyses. In our tests, LDH-Cytotoxicity Assay Kit (BioVision) was used. The toxic eff ect of drugs was measured in the developed microsystem made of PDMS (poly(dimethylsiloxane)). Analytical reaction took place in the special designed microchannel geometry. Then, the LDH activity was measured at 490 nm using spectrophotometer. In subsequent experiments, appropriate conditions for measurements using a microfl uidic system were chosen. It was found that the selected reagent is sensitive to temperature changes and light exposure. Reaction time in the microsystem was determined by changes of fl ow rates of reagents. Afterwards, for the various reaction time, the toxic eff ect of 5-fl uorouracil, celecoxib and 1,4-dioxane was performed. The obtained results were compared with the results carried out in 96-well plates. Based on these results, it was noted that the enzymatic reaction time in the microsystem is shorter than in 96-well plate. Moreover, the advantage of using microsystem is also the small amount of reagents.
Źródło:: Challenges of Modern Technology; 2012, 3, 4; 3-8
2082-2863
2353-4419
Pojawia się w:: Challenges of Modern Technology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Microfluidic devices — application in anticancer studies
Autorzy:: Jędrych, E.
Chudy, M.
Dybko, A.
Brzózka, Z.
Powiązania:: https://bibliotekanauki.pl/articles/115929.pdf
Data publikacji:: 2012
Wydawca:: Fundacja na Rzecz Młodych Naukowców
Tematy:: microfluidic system
PDMS
adherent cell culture
concentration gradient generator (CGG)
cytotoxicity tests
photodynamic therapy (PDT) procedures
Opis:: A rapidly growing pharmaceutical industry requires faster and more efficient ways to find and test new drugs. One of the new method for cell culture and examining the toxic effects of drugs is application of microfluidic systems. They provide new types of microenvironments and new methods for investigation of anticancer therapy. The use of microsystems is a solution that gives the opportunity to reduce not only cost and time, but also a number of tests on animals. In this paper we present designed and fabricated hybrid microfluidic systems which are applicable for cell culture, cell based cytotoxicity assays and photodynamic therapy procedures. Polydimethylsiloxane (PDMS) and sodium glass were used for fabrication of microdevices. The designed geometry of the microdevices includes cell culture microchambers and a concentration gradient generator (CGG). The CGG enables to obtain diff erent concentrations of tested drugs in a single step, which is a significant simplification of cytotoxicity assay procedure. In the designed microsystems three various cell lines (normal and carcinoma) were cultured and analyzed.
Źródło:: Challenges of Modern Technology; 2012, 3, 2; 3-5
2082-2863
2353-4419
Pojawia się w:: Challenges of Modern Technology
Dostawca treści:: Biblioteka Nauki

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