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Wyszukujesz frazę "metoda nested-PCR" wg kryterium: Temat


Wyświetlanie 1-7 z 7
Tytuł:
NESTED PCR w diagnostyce przypadkow pneumocystozowego zapalenia pluc [PCP] u pacjentow z Aids
Autorzy:
Gołąb, E.
Sobolewska, A.
Bitkowska, E.
Bednarska, A.
Berak, H.
Dzbeński, T.H.
Horban, A.
Powiązania:
https://bibliotekanauki.pl/articles/2148066.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
zapalenie pluc
metody diagnostyczne
czynniki chorobotworcze
choroby czlowieka
parazytologia lekarska
lancuchowa reakcja polimerazy
pneumocystoza
diagnostyka
AIDS
ludzie chorzy
Pneumocystis carinii
metoda nested-PCR
Opis:
The studies were undertaken to evaluate the usefulness of a nested PCR assay in the diagnosis of Pneumocystis carinii pneumonia (PCP) in AIDS patients. To achieve the end, 51 excretions samples from the respiratory traci were collected from HIV-infected patients with respiratory symptoms, and examined for the presence of specific DNA. A portion of the mitochondrial large-subunit rRNA gene of Pneumocystis carinii was amplified with outer primers pair pAZ 102E, pAZ 102H and internal primers pAZ 102X, pAZ 102Y. Positive nested PCR results were obtained with 36 out of 51 examined samples. Some 52.8% of the positive results were obtained with samples collected from patients without clinical diagnosis of PCP. It was concluded, that the nested PCR method, being too sensitive, is not suitable for the routine diagnosis of PCP in AIDS patients.
Źródło:
Wiadomości Parazytologiczne; 2001, 47, 3; 521-525
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
NESTED PCR w diagnostyce przypadkow pneumocystozowego zapalenia pluc [PCP] u pacjentow z Aids
Autorzy:
Golab, E
Sobolewska, A.
Bitkowska, E.
Bednarska, A.
Berak, H.
Dzbenski, T.H.
Horban, A.
Powiązania:
https://bibliotekanauki.pl/articles/840596.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
zapalenie pluc
metody diagnostyczne
czynniki chorobotworcze
choroby czlowieka
parazytologia lekarska
lancuchowa reakcja polimerazy
pneumocystoza
diagnostyka
AIDS
ludzie chorzy
Pneumocystis carinii
metoda nested-PCR
Opis:
The studies were undertaken to evaluate the usefulness of a nested PCR assay in the diagnosis of Pneumocystis carinii pneumonia (PCP) in AIDS patients. To achieve the end, 51 excretions samples from the respiratory traci were collected from HIV-infected patients with respiratory symptoms, and examined for the presence of specific DNA. A portion of the mitochondrial large-subunit rRNA gene of Pneumocystis carinii was amplified with outer primers pair pAZ 102E, pAZ 102H and internal primers pAZ 102X, pAZ 102Y. Positive nested PCR results were obtained with 36 out of 51 examined samples. Some 52.8% of the positive results were obtained with samples collected from patients without clinical diagnosis of PCP. It was concluded, that the nested PCR method, being too sensitive, is not suitable for the routine diagnosis of PCP in AIDS patients.
Źródło:
Annals of Parasitology; 2001, 47, 3; 521-525
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Metody molekularne i immunologiczne stosowane w diagnostyce grzybic
Molecular and immunological methods applied in diagnosis of mycoses
Autorzy:
Kuba, K
Powiązania:
https://bibliotekanauki.pl/articles/841048.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
diagnostyka lekarska
diagnostyka molekularna
metody diagnostyczne
diagnostyka immunologiczna
metody molekularne
metoda nested-PCR
testy serologiczne
metoda EIA
metoda RT-PCR
metoda RAPD
markery molekularne
metoda RFLP
lancuchowa reakcja polimerazy
metoda AFLP
metody immunologiczne
grzybice
Źródło:
Annals of Parasitology; 2008, 54, 3; 187-197
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Metody molekularne i immunologiczne stosowane w diagnostyce grzybic
Molecular and immunological methods applied in diagnosis of mycoses
Autorzy:
Kuba, K.
Powiązania:
https://bibliotekanauki.pl/articles/2143775.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
diagnostyka lekarska
diagnostyka molekularna
metody diagnostyczne
diagnostyka immunologiczna
metody molekularne
metoda nested-PCR
testy serologiczne
metoda EIA
metoda RT-PCR
metoda RAPD
markery molekularne
metoda RFLP
lancuchowa reakcja polimerazy
metoda AFLP
metody immunologiczne
grzybice
Opis:
The diagnosis of fungal infections remains a problem for the management of fungal diseases, particularly in the immunocompromised patients. Systemic Candida infections and invasive aspergillosis can be a serious problem for individuals who need intensive care. Traditional methods used for the identification and typing of medically important fungi, such as morphological and biochemical analysis, are time−consuming. For the diagnosis of mycoses caused by pathogenic fungi faster and more specific methods, especially after the dramatic increase in nosocomial invasive mycoses are needed. New diagnostic tools to detect circulating fungal antigens in biological fluids and PCR−based methods to detect species or genus−specific DNA or RNA have been developed. Antigen detection is limited to searching only one genus. Molecular genetic methods, especially PCR analysis, are becoming increasingly important as a part of diagnostics in the clinical mycology laboratory. Various modifications of the PCR method are used to detect DNA in clinical material, particularly multiple, nested and real−time PCR. Molecular methods may be used to detection of nucleic acids of fungi in clinical samples, to identify fungal cultures at the species level or to evaluate strain heterogeneity differences within the species. This article reviews some of the recent advances in the possibility of molecular diagnosis of fungal infections.
Źródło:
Wiadomości Parazytologiczne; 2008, 54, 3; 187-197
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Metody identyfikacji gatunkowej grzybów z rodzaju Candida. Część II. Techniki molekularne
Methods for species identification of genus Candida fungi. Part II. Molecular techniques
Autorzy:
Gnat, Sebastian
Powiązania:
https://bibliotekanauki.pl/articles/22326536.pdf
Data publikacji:
2022
Wydawca:
Krajowa Izba Lekarsko-Weterynaryjna
Tematy:
choroby grzybicze
grzybice
Candida
identyfikacja gatunkowa
metody
metody molekularne
test PCR
metoda multiplex PCR
metoda nested-PCR
metoda real time PCR
fluorescencyjna hybrydyzacja in situ
spektrometria mas MALDI-TOF MS
czynniki chorobotwórcze
grzyby chorobotwórcze
łańcuchowa reakcja polimerazy
piroliza ze spektometrią mas
identification
molecular techniques
MALDI-TOF MS
Opis:
A rapid and accurate identification of the Candida is crucial for clinical treatment of local and systemic candidiasis. Premature diagnosis of invasive fungal infections is problematic because most clinical signs are non-specific and cultures are often negative or become positive too late for the initiation of effective antifungal therapy. Therefore, studies have been performed to improve molecular techniques for the diagnosis of candidiasis. Today, molecular strategies, such as PCR and non-PCR based methods are used to complement conventional laboratory approach. They provide accurate results in much short time of 1.5–3 h. Given the high accuracy and time saving with molecular typing techniques it is likely that most of these methods could improve routine clinical laboratory identification of Candida yeast species. However, further studies are needed for standardization of such procedures. This article presents an overview and discussion on molecular identification methods in the context of their application for the diagnosis of Candida fungi. Particular attention has been focused on the advantages and limitations of the indicated methods and the possibilities of their implementation for routine use in clinical laboratories.
Źródło:
Życie Weterynaryjne; 2022, 97, 03; 167-174
0137-6810
Pojawia się w:
Życie Weterynaryjne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Detekcja fitopatogenów z rodzaju Phytophthora w glebach leśnych za pomocą analiz DNA
Detection of Phytophthora in forest soils using DNA analysis
Autorzy:
Kubiak, K.A.
Oszako, T.
Jabłoński, T.
Powiązania:
https://bibliotekanauki.pl/articles/996874.pdf
Data publikacji:
2012
Wydawca:
Polskie Towarzystwo Leśne
Tematy:
lesnictwo
gleby lesne
czynniki chorobotworcze
mikroorganizmy glebowe
Phytophthora
identyfikacja
analiza DNA
metoda nested-PCR
phytophthora
identyfication
dna isolation
forest soil
pcr
Opis:
Pathogenic oomycetes from the genus Phytophthora attack the base trunks and root systems of trees causing their illness and dying. Under favorable conditions, they may cause damage to even 90% of fine roots. For this reason, they are a particular threat to seedlings in nurseries of forest trees and ornamental plants. Early detection and identification of Phytophthora species is a key issue in forest protection. The aim of this study was to compare the two methods of identification of pathogenic Phytophthora in soil samples collected around 50 selected oaks in Krotoszyn and Karczma Borowa forest districts. Each of the soil samples were analyzed in parallel: 1) direct isolation of genomic DNA from soil and 2) isolation of genomic DNA from soil, preceded by its three day pre−incubation in a selective medium for Phytophthora. Species identification was performed by PCR amplification with primers specific for the species of Phytophthora. The results indicate that the use of pre−incubation phase of soil in a medium−PARP PeaBroth before isolation of DNA, increases the sensitivity of detection of these phytopathogens using PCR. With pre−incubation, the method revealed 54% of positive findings, while simultaneously conducted the same analysis of soil samples by using only direct DNA isolation from soil and PCR amplification provided only 30% of positive findings.
Źródło:
Sylwan; 2012, 156, 06; 437-443
0039-7660
Pojawia się w:
Sylwan
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-7 z 7

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