Temat: metoda Real-Time PCR - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Metody molekularne wykorzystywane w identyfikacji pasozytow
Autorzy:: Kamola, D.
Prostek, A.
Powiązania:: https://bibliotekanauki.pl/articles/836397.pdf
Data publikacji:: 2010
Wydawca:: Polskie Towarzystwo Parazytologiczne
Tematy:: pasozyty
identyfikacja
metody molekularne
lancuchowa reakcja polimerazy
metoda Real Time PCR
Źródło:: Annals of Parasitology; 2010, 56, 3; 221
0043-5163
Pojawia się w:: Annals of Parasitology
Dostawca treści:: Biblioteka Nauki

Artykuł

Zmień widok

na półce

Skocz do pozycji: 2.

Tytuł:: Monitorowanie chorób wywołanych przez patogeniczne lęgniowce za pomocą analiz DNA
Monitoring of diseases caused by pathogenic oomycetes using DNA analysis
Autorzy:: Nevoigt, F.
Oszako, T.
Nowakowska, J.A.
Powiązania:: https://bibliotekanauki.pl/articles/1008933.pdf
Data publikacji:: 2010
Wydawca:: Polskie Towarzystwo Leśne
Tematy:: fitopatologia
czynniki chorobotworcze
grzyby
legniowce
Oomycetes
choroby grzybowe
monitoring
Phytophthora
Pythium
diagnostyka molekularna
analiza DNA
metoda real time PCR
phytophthora spp.
real time pcr
its−dna molecular diagnostics
baiting
rhododendrons
Opis:: Phytophthora species are best known as pathogens of agricultural crops (e.g. P. infestans), but there are also invasive pathogens destroying forest atands (e.g. P. ramorum in the USA) or even whole forest ecosystems (e.g. P. cinnamomi in Australia). Still, little is known about indiginous species, especially in wild ecosystems. Rhododendrons are well known as a reservuar for oomycetes’ development. The main objectives of the present study were to develop the new tool for the identification of pathogenic Phytophthora and Phytium based on DNA sequence analysis of the ITS1, 5.8S gene and ITS2 region. Rhododendrons leaves served as specific plant baits. In order to reach the goal the real time PCR, the nested PCR and the DNA sequencing of the rDNA ITS region were carried out. The genomic DNA was extracted form the symptomatic rhododendron leaves. Three distinct Oomycetes species: Phytophthora cactorum, Pythium mercuriale and em>Pythium recalcitrans were detected in rhododendron leaves and registered in GenBank.
Źródło:: Sylwan; 2010, 154, 07; 450-455
0039-7660
Pojawia się w:: Sylwan
Dostawca treści:: Biblioteka Nauki

Artykuł

Zmień widok

na półce

Skocz do pozycji: 3.

Tytuł:: Molekularna diagnostyka wybranych patogenów z rodzaju Phytophthora w ramach integrowanej ochrony roślin
Molecular diagnostic of Phytophthora pathogens as a tool for Integrated Pest Management
Autorzy:: Nowakowska, J.A.
Malewski, T.
Tereba, A.
Borys, M.
Oszako, T.
Powiązania:: https://bibliotekanauki.pl/articles/989551.pdf
Data publikacji:: 2016
Wydawca:: Polskie Towarzystwo Leśne
Tematy:: fitopatologia
Phytophthora
wykrywanie
identyfikacja
Phytophthora alni subsp.multiformis
Phytophthora lacustris
Phytophthora taxon hungarica
metody badan
metoda real time PCR
sondy TaqMan
real time pcr
taqman
butt and fine root pathogens
phytophthora
Opis:: Traditional detection methods such as baiting or direct isolation take a long time and are incapable to handling large volume of material to be tested. The real−time PCR−based techniques are faster, more sensitive, more easily automated, and do not require post−amplification procedures. Species−specific primers for Phytophthora were designed based on the internal transcribed spacer regions (ITS) of rDNA collected from the NCBI DNA database. Primers and probes were designed using the Allele ID 7 at default search criteria. Specific probes were labeled with the reporter dyes JOE (6−carboxy−4,5−dichloro−2,7−dimethoxyfluorescein) at the 5' end and HBQ1 quencher at the 3' end (Sigma−Aldrich). The specificity of primers and fluorogenic probes was tested against genomic DNA of P. alni subsp. multiformis, P. lacustris and P. taxon hungarica. The real−time PCR reactions with the specific probes and primers yielded positive results with five concentrations of standards obtained by standard PCR reaction for corresponding Phytophthora species. The negative control (lack of DNA pathogens) yielded no amplification products. Standard curves showed a linear correlation between input DNA and cycle threshold (Ct) values with R² from 0.994 (P. alni) to 0.998 (P. taxon hungarica). The amplification efficiency of target DNA varied from 94.6% (P. alni) to 100% (P. taxon hungarica). The validation of the primers and probes designed for analysed Phytophthora species was performed on pure cultures, on soil samples from the forest nursery and declining oak stands. The designed probes displayed the high specificity of the detection of investigated species in pure cultures. The presented new molecular TaqMan probes can fully assist the integrated pest management as a powerful tool for a quick detection of above pathogenic organisms in forest nurseries. The molecular detection of harmful phytophthoras and in consequences diminishing of fungicides use for their control in forestry fully support European Union directives as well as the ‘Good plant protection practice measures' elaborated by European and Mediterranean Organisation of Plant Protection.
Źródło:: Sylwan; 2016, 160, 05; 365-370
0039-7660
Pojawia się w:: Sylwan
Dostawca treści:: Biblioteka Nauki

Artykuł

Zmień widok

na półce

Skocz do pozycji: 4.

Tytuł:: Metody identyfikacji gatunkowej grzybów z rodzaju Candida. Część II. Techniki molekularne
Methods for species identification of genus Candida fungi. Part II. Molecular techniques
Autorzy:: Gnat, Sebastian
Powiązania:: https://bibliotekanauki.pl/articles/22326536.pdf
Data publikacji:: 2022
Wydawca:: Krajowa Izba Lekarsko-Weterynaryjna
Tematy:: choroby grzybicze
grzybice
Candida
identyfikacja gatunkowa
metody
metody molekularne
test PCR
metoda multiplex PCR
metoda nested-PCR
metoda real time PCR
fluorescencyjna hybrydyzacja in situ
spektrometria mas MALDI-TOF MS
czynniki chorobotwórcze
grzyby chorobotwórcze
łańcuchowa reakcja polimerazy
piroliza ze spektometrią mas
identification
molecular techniques
MALDI-TOF MS
Opis:: A rapid and accurate identification of the Candida is crucial for clinical treatment of local and systemic candidiasis. Premature diagnosis of invasive fungal infections is problematic because most clinical signs are non-specific and cultures are often negative or become positive too late for the initiation of effective antifungal therapy. Therefore, studies have been performed to improve molecular techniques for the diagnosis of candidiasis. Today, molecular strategies, such as PCR and non-PCR based methods are used to complement conventional laboratory approach. They provide accurate results in much short time of 1.5–3 h. Given the high accuracy and time saving with molecular typing techniques it is likely that most of these methods could improve routine clinical laboratory identification of Candida yeast species. However, further studies are needed for standardization of such procedures. This article presents an overview and discussion on molecular identification methods in the context of their application for the diagnosis of Candida fungi. Particular attention has been focused on the advantages and limitations of the indicated methods and the possibilities of their implementation for routine use in clinical laboratories.
Źródło:: Życie Weterynaryjne; 2022, 97, 03; 167-174
0137-6810
Pojawia się w:: Życie Weterynaryjne
Dostawca treści:: Biblioteka Nauki