Temat: mRNA expression - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Analysis of integrins and vascular endothelial growth factor isoforms mRNA expression in the canine uterus during perimplantation period
Autorzy:: Bukowska, D.
Kempisty, B.
Jackowska, M.
Wozna, M.
Antosik, P.
Piotrowska, H.
Jaskowski, J.M.
Powiązania:: https://bibliotekanauki.pl/articles/31799.pdf
Data publikacji:: 2011
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: integrin
vascular endothelial growth factor
isoform
mRNA expression
dog
uterus
pregnancy
bitch
Opis:: Integrins are the major receptors within the extracellular matrix (ECM) that mediate several functions connected with cell life and metabolism, such as cell adhesion, migration, cytoskeletal organization, proliferation, survival, and differentiation. A vascular endothelial growth factor (VEGF) is one of the most important angiogenic factors. It has been suggested that the expression of this gene may play crucial physiological roles in reproductive organs. All investigated endometrial tissues were isolated on day 10-12 after mating. Control bitches, used in this study, were in metestrus, which was determined according to the vaginal cytology and progesterone level in blood. Early pregnancy was verified by flushing the uterine horns with PBS. Total RNA was isolated from the bitches endometrium by means of the Chomczyński and Sacchi method, treated by DNase I, and reverse-transcribed into cDNA. A quantitative analysis of integrins α2b, β2 and β3, VEGF 164, 182 and 188 cDNA was performed by RT-PCR. In results we have shown an increased expression of all investigated genes (integrins α2b, β2 and β3, VEGF 164, 182, and 188) in pregnant bitches uterus as compared to non-pregnant females (P<0.001). Our results indicated that the expression of genes encoding integrins and vascular endothelial growth factors is different in relation to the time of the embryo implantation and it is increased in the first period of this process. This may be associated with the induction of specific mechanisms responsible for receptivity of uterus following the embryo attachment. In addition, all of investigated genes are up-regulated in a pregnancy-specific manner and the increased expression of these genes may regulate the uterus function during the implantation of canine embryos.
Źródło:: Polish Journal of Veterinary Sciences; 2011, 14, 2
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Metabolic enzymes that bind RNA: yet another level of cellular regulatory network?
Autorzy:: Cieśla, Joanna
Powiązania:: https://bibliotekanauki.pl/articles/1041266.pdf
Data publikacji:: 2006
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: enzymes
gene expression
mRNA binding
translation regulation
Opis:: Several enzymes that were originally characterized to have one defined function in intermediatory metabolism are now shown to participate in a number of other cellular processes. Multifunctional proteins may be crucial for building of the highly complex networks that maintain the function and structure in the eukaryotic cell possessing a relatively low number of protein-encoding genes. One facet of this phenomenon, on which I will focus in this review, is the interaction of metabolic enzymes with RNA. The list of such enzymes known to be associated with RNA is constantly expanding, but the most intriguing question remains unanswered: are the metabolic enzyme-RNA interactions relevant in the regulation of cell metabolism? It has been proposed that metabolic RNA-binding enzymes participate in general regulatory circuits linking a metabolic function to a regulatory mechanism, similar to the situation of the metabolic enzyme aconitase, which also functions as iron-responsive RNA-binding regulatory element. However, some authors have cautioned that some of such enzymes may merely represent "molecular fossils" of the transition from an RNA to a protein world and that the RNA-binding properties may not have a functional significance. Here I will describe enzymes that have been shown to interact with RNA (in several cases a newly discovered RNA-binding protein has been identified as a well-known metabolic enzyme) and particularly point out those whose ability to interact with RNA seems to have a proven physiological significance. I will also try to depict the molecular switch between an enzyme's metabolic and regulatory functions in cases where such a mechanism has been elucidated. For most of these enzymes relations between their enzymatic functions and RNA metabolism are unclear or seem not to exist. All these enzymes are ancient, as judged by their wide distribution, and participate in fundamental biochemical pathways.
Źródło:: Acta Biochimica Polonica; 2006, 53, 1; 11-32
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Characterization and expression analysis of the yellow lupin (Lupinus luteus L.) gene coding for nodule specific proline-rich protein.
Autorzy:: Karłowski, Wojciech
Stróżycki, Paweł
Legocki, Andrzej
Powiązania:: https://bibliotekanauki.pl/articles/1044363.pdf
Data publikacji:: 2000
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: symbiotic nitrogen fixation
plant gene expression
ENOD2
proline-rich proteins
early nodulins
mRNA degradation
Opis:: The LlPRP2 gene coding for a proline-rich protein shows a high level of similarity to, as well as significant differences from the family of ENOD2 nodule-specific genes. Several sequence motifs with putative regulatory function were identified in the 5' and 3' noncoding regions of the LlPRP2 gene. Northern blot analysis revealed that the expression of the LlPRP2 gene begins 9 days after inoculation of yellow lupin roots with Bradyrhizobium sp. (Lupinus); the expression is restricted to symbiotic nodules and is not detected in other tissues or organs. Detailed hybridization analysis showed that, when expression is activated, the LlPRP2 transcript is modified so as to produce at least three bands and a continuous distribution of decay intermediates. The modification of the LlPRP2 transcript probably involves degradation from the 5'- and/or 3'-ends of the RNA molecules. Southern blot analysis indicates that only one gene is present in the yellow lupin genome. The presence of genes homologous to the LlPRP2 gene was confirmed for three cultivars of yellow lupin and for Lupinus angustifolius. However, LlPRP2 homologues were not detected in Lupinus albus cv. Bac, indicating that this plant may lack the ENOD2 sequence.
Źródło:: Acta Biochimica Polonica; 2000, 47, 2; 371-383
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Infection with Echinococcus granulosus [Batsch, 1786] and expression of superoxide dismutase gene at mRNA level in hepatocytes
Autorzy:: Kozlowska-Loj, J
Rzymowska, J.
Powiązania:: https://bibliotekanauki.pl/articles/839774.pdf
Data publikacji:: 2006
Wydawca:: Polskie Towarzystwo Parazytologiczne
Tematy:: parasitic disease
pig
Echinococcus granulosus
superoxide dismutase
infection
gene expression
mRNA level
animal disease
organism
parasite
detoxication
parasitology
hepatocyte
Opis:: Superoxide dismutase (SOD) plays an important role in detoxication of the organism. Its function is to protect the organism against the cytotoxic action of free radicals. The highest expression of superoxide dismutase was observed in the hepatocytes adjacent to the hydatid cyst. The expression of this gene in the hepatocytes of infected livers 5 cm apart from the infection site was slightly lower.
Źródło:: Annals of Parasitology; 2006, 52, 4
0043-5163
Pojawia się w:: Annals of Parasitology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: Infection with Echinococcus granulosus [Batsch, 1786] and expression of superoxide dismutase gene at mRNA level in hepatocytes
Autorzy:: Kozłowska-Łój, J.
Rzymowska, J.
Powiązania:: https://bibliotekanauki.pl/articles/2146352.pdf
Data publikacji:: 2006
Wydawca:: Polskie Towarzystwo Parazytologiczne
Tematy:: parasitic disease
pig
Echinococcus granulosus
superoxide dismutase
infection
gene expression
mRNA level
animal disease
organism
parasite
detoxication
parasitology
hepatocyte
Opis:: Superoxide dismutase (SOD) plays an important role in detoxication of the organism. Its function is to protect the organism against the cytotoxic action of free radicals. The highest expression of superoxide dismutase was observed in the hepatocytes adjacent to the hydatid cyst. The expression of this gene in the hepatocytes of infected livers 5 cm apart from the infection site was slightly lower.
Źródło:: Wiadomości Parazytologiczne; 2006, 52, 4; 287-289
0043-5163
Pojawia się w:: Wiadomości Parazytologiczne
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 6.

Tytuł:: Detekcja ekspresji genów drzew leśnych za pomocą mikromacierzy DNA
Gene-expression in forest-tree species assessed with microarrays as a tool
Autorzy:: Nowakowska, J.A.
Powiązania:: https://bibliotekanauki.pl/articles/972859.pdf
Data publikacji:: 2006
Wydawca:: Polskie Towarzystwo Leśne
Tematy:: mRNA
ekspresja genow
genetyka roslin
mikromacierze DNA
sekwencja ESTs
lesnictwo
wykrywanie
drzewa lesne
microarray
gene−expression
forest−tree specie
ESTs
Opis:: DNA microarray technology is a powerful tool in functional genomics study of many organisms, the forest−trees included. This method allow to examine simultaneously the changes in expression of thousands of genes and it is based on specific hybridization of cDNA probes from an organism with the DNA library immobilized in an array. The power of this method consists in miniaturization, automation and parallel study of large−scale genome from multiple samples. In forest science, the microarray technology has already been applied in some study of gene−expression in Populus, Pinus and Picea species and the number of new reports is still increasing every year. Since far, some gene−expression have been studied among woody plants in regard of development and growth processes (xylem, adventious−root and zygotic embryo formation, flowering, ripening, shooting of leaves), resistance mechanisms against biotic (fungi, viruses) and abiotic factors (drought, NaCl, elevated CO2 and O3 concentrations). Many practical applications of the microarray technique may concern the early selection in nursery of trees for morphologically valuable traits, the sustainable forest regeneration and the production of genetically transformed species for the chosen trait.
Źródło:: Sylwan; 2006, 150, 04; 33-43
0039-7660
Pojawia się w:: Sylwan
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 7.

Tytuł:: Tumor necrosis factor-alpha as a possible auto-/paracrine factor affecting estrous cycle in the cat uterus
Autorzy:: Siemieniuch, M.J.
Ogrodowska, K.
Ohgawara, H.
Skarzynski, D.J.
Okuda, K.
Powiązania:: https://bibliotekanauki.pl/articles/31521.pdf
Data publikacji:: 2010
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: oestrous cycle
cat
tumour necrosis factor-alpha
uterus
prostaglandin
tumour cell
cell differentiation
mRNA
gene expression
Opis:: Tumor Necrosis Factor-alpha (TNFα) is a pleiotrophic cytokine, affects either normal or tumor cells, and influences cellular differentiation. TNFα role in female reproduction has been proven to be mediated through an influence on prostaglandin (PGs) synthesis and output. To evaluate the possible role of TNFα in an auto-/paracrine regulation in the cat uterus, mRNA expression coding for TNFα and its receptors (TNFR1 and TNFR2), and TNFα protein content at different stages of the estrous cycle were investigated. Additionally, TNFα involvement in PG secretion at different stages of the estrous cycle was investigated by in vitro tissue culture. Gene expressions coding for TNFα and TNFR1 were the highest at diestrus (P < 0.05). TNFα protein expression was the lowest at interestrus (P < 0.05). Nevertheless, TNFR2 was not affected by the estrous stage. TNFα at a dose of 1 ng/ml significantly increased PGF₂α secretion at estrus (P < 0.01) and PGE₂ secretion at diestrus (P < 0.001) after 12h incubation. Overall findings indicate that TNFα locally produced in the cat’s uterus, stimulates PG secretion in an estrous cycle-related manner.
Źródło:: Polish Journal of Veterinary Sciences; 2010, 13, 4
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 8.

Tytuł:: Preliminary study on adverse effects of phenanthrene and its methyl and phenyl derivatives in larval zebrafish, Danio rerio
Autorzy:: Wolińska, L.
Brzuzan, P.
Woźny, M.
Góra, M.
Łuczyński, M. K.
Podlasz, P.
Kolwicz, S.
Piasecka, A.
Powiązania:: https://bibliotekanauki.pl/articles/363152.pdf
Data publikacji:: 2011
Wydawca:: Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:: toksyczność ostra
CYP1A
cyp1b1
geny vtg
ekspresja mRNA
stężenie nie wywołujące efektu
WWA
Real-Time qPCR
acute toxicity
cyp1a genes
cyp1b1 genes
vtg genes
mRNA expression
No Effect Concentration
PAHs
Opis:: Toxic effects of polycyclic aromatic hydrocarbons (PAHs) have been extensively studied in fish, although knowledge concerning biological activities of phenanthrene and its derivatives remains still incomplete. The aim of this work was to evaluate lethal and sublethal effects of benzo(a)pyrene, phenanthrene and phenanthrene derivatives (1-methylphenanthrene, 4-methylphenanthrene, 1-phenylphenanthrene and 4-phenylphenanthrene) on zebrafish (Danio rerio) larvae. We conducted acute toxicity test, using 96h static renewal exposure to a series of the PAH concentrations (0.05, 0.50, 5.00, 50.00µmol*l-1), to determine the No Effect Concentration (NEC) value for each compound examined. The mean NEC estimates obtained in the study were 5.16۪.45µmol*l-1 (B[a]P), 4.88۪.13µmol*l-1 (Ph), 40.24䔰.93µmol*l-1 (1P-Ph), 47.92ۭ.61µmol*l-1 (1M-Ph), 24.31۱.33µmol*l-1 (4P-Ph) and 3.11۫.01µmol*l-1 (4M-Ph) and suggested the following order of PAH toxicities on Danio rerio larvae: 4M-Ph>Ph˜B[a]P>4PPhP-Ph>1M-Ph. To gain insight into possible molecular mechanisms of apparent toxicity of phenanthrene derivatives on zebrafish larvae, we examined mRNA expression of cyp1a, cyp1b1, and vtg genes in the larvae exposed for 48h to a PAH concentration of 0.50µmol*l-1. Whereas the larvae exposed to each tested PAH displayed many developmental abnormalities (i.e. pericardial and yolk sac edema, dorsal curvature, or tail malformations), no significant upregulation of cyp1a and cyp1b1 mRNA was observed, except for zebrafish exposed to B[a]P. However, significant reduction of vtg mRNA was observed in the larvae exposed to phenanthrene and its 4P- derivative. The results may contribute to the development of a new knowledge about effects of structurally diverse phenanthrene derivatives on vertebrate organisms.
Źródło:: Environmental Biotechnology; 2011, 7, 1; 26-33
1734-4964
Pojawia się w:: Environmental Biotechnology
Dostawca treści:: Biblioteka Nauki