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    Wyświetlanie 1-9 z 9
    Tytuł:
    Analysis of integrins and vascular endothelial growth factor isoforms mRNA expression in the canine uterus during perimplantation period
    Autorzy:
    Bukowska, D.
    Kempisty, B.
    Jackowska, M.
    Wozna, M.
    Antosik, P.
    Piotrowska, H.
    Jaskowski, J.M.
    Powiązania:
    https://bibliotekanauki.pl/articles/31799.pdf
    Data publikacji:
    2011
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    integrin
    vascular endothelial growth factor
    isoform
    mRNA expression
    dog
    uterus
    pregnancy
    bitch
    Opis:
    Integrins are the major receptors within the extracellular matrix (ECM) that mediate several functions connected with cell life and metabolism, such as cell adhesion, migration, cytoskeletal organization, proliferation, survival, and differentiation. A vascular endothelial growth factor (VEGF) is one of the most important angiogenic factors. It has been suggested that the expression of this gene may play crucial physiological roles in reproductive organs. All investigated endometrial tissues were isolated on day 10-12 after mating. Control bitches, used in this study, were in metestrus, which was determined according to the vaginal cytology and progesterone level in blood. Early pregnancy was verified by flushing the uterine horns with PBS. Total RNA was isolated from the bitches endometrium by means of the Chomczyński and Sacchi method, treated by DNase I, and reverse-transcribed into cDNA. A quantitative analysis of integrins α2b, β2 and β3, VEGF 164, 182 and 188 cDNA was performed by RT-PCR. In results we have shown an increased expression of all investigated genes (integrins α2b, β2 and β3, VEGF 164, 182, and 188) in pregnant bitches uterus as compared to non-pregnant females (P<0.001). Our results indicated that the expression of genes encoding integrins and vascular endothelial growth factors is different in relation to the time of the embryo implantation and it is increased in the first period of this process. This may be associated with the induction of specific mechanisms responsible for receptivity of uterus following the embryo attachment. In addition, all of investigated genes are up-regulated in a pregnancy-specific manner and the increased expression of these genes may regulate the uterus function during the implantation of canine embryos.
    Źródło:
    Polish Journal of Veterinary Sciences; 2011, 14, 2
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Gonadotropin-releasing hormone and kisspeptin-10 regulate nuclear receptor subfamily 5 group a member 1/catenin beta 1/nuclear receptor subfamily 0 group B member 1 activity in female rat anterior pituitary gland
    Autorzy:
    Zielinska-Gorska, M.
    Gorski, K.
    Biernacka, K.
    Sawosz, E.
    Kaminski, T.
    Gajewska, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/69453.pdf
    Data publikacji:
    2018
    Wydawca:
    Polskie Towarzystwo Fizjologiczne
    Tematy:
    gonadotrophin releasing hormone
    neuronal network
    mRNA expression
    kisspeptin-10
    nuclear receptor
    anterior pituitary gland
    ovariectomy
    luteinizing hormone
    rat
    Źródło:
    Journal of Physiology and Pharmacology; 2018, 69, 3
    0867-5910
    Pojawia się w:
    Journal of Physiology and Pharmacology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Preliminary study on adverse effects of phenanthrene and its methyl and phenyl derivatives in larval zebrafish, Danio rerio
    Autorzy:
    Wolińska, L.
    Brzuzan, P.
    Woźny, M.
    Góra, M.
    Łuczyński, M. K.
    Podlasz, P.
    Kolwicz, S.
    Piasecka, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/363152.pdf
    Data publikacji:
    2011
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    toksyczność ostra
    CYP1A
    cyp1b1
    geny vtg
    ekspresja mRNA
    stężenie nie wywołujące efektu
    WWA
    Real-Time qPCR
    acute toxicity
    cyp1a genes
    cyp1b1 genes
    vtg genes
    mRNA expression
    No Effect Concentration
    PAHs
    Opis:
    Toxic effects of polycyclic aromatic hydrocarbons (PAHs) have been extensively studied in fish, although knowledge concerning biological activities of phenanthrene and its derivatives remains still incomplete. The aim of this work was to evaluate lethal and sublethal effects of benzo(a)pyrene, phenanthrene and phenanthrene derivatives (1-methylphenanthrene, 4-methylphenanthrene, 1-phenylphenanthrene and 4-phenylphenanthrene) on zebrafish (Danio rerio) larvae. We conducted acute toxicity test, using 96h static renewal exposure to a series of the PAH concentrations (0.05, 0.50, 5.00, 50.00µmol*l-1), to determine the No Effect Concentration (NEC) value for each compound examined. The mean NEC estimates obtained in the study were 5.16۪.45µmol*l-1 (B[a]P), 4.88۪.13µmol*l-1 (Ph), 40.24䔰.93µmol*l-1 (1P-Ph), 47.92ۭ.61µmol*l-1 (1M-Ph), 24.31۱.33µmol*l-1 (4P-Ph) and 3.11۫.01µmol*l-1 (4M-Ph) and suggested the following order of PAH toxicities on Danio rerio larvae: 4M-Ph>Ph˜B[a]P>4PPhP-Ph>1M-Ph. To gain insight into possible molecular mechanisms of apparent toxicity of phenanthrene derivatives on zebrafish larvae, we examined mRNA expression of cyp1a, cyp1b1, and vtg genes in the larvae exposed for 48h to a PAH concentration of 0.50µmol*l-1. Whereas the larvae exposed to each tested PAH displayed many developmental abnormalities (i.e. pericardial and yolk sac edema, dorsal curvature, or tail malformations), no significant upregulation of cyp1a and cyp1b1 mRNA was observed, except for zebrafish exposed to B[a]P. However, significant reduction of vtg mRNA was observed in the larvae exposed to phenanthrene and its 4P- derivative. The results may contribute to the development of a new knowledge about effects of structurally diverse phenanthrene derivatives on vertebrate organisms.
    Źródło:
    Environmental Biotechnology; 2011, 7, 1; 26-33
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Metabolic enzymes that bind RNA: yet another level of cellular regulatory network?
    Autorzy:
    Cieśla, Joanna
    Powiązania:
    https://bibliotekanauki.pl/articles/1041266.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    enzymes
    gene expression
    mRNA binding
    translation regulation
    Opis:
    Several enzymes that were originally characterized to have one defined function in intermediatory metabolism are now shown to participate in a number of other cellular processes. Multifunctional proteins may be crucial for building of the highly complex networks that maintain the function and structure in the eukaryotic cell possessing a relatively low number of protein-encoding genes. One facet of this phenomenon, on which I will focus in this review, is the interaction of metabolic enzymes with RNA. The list of such enzymes known to be associated with RNA is constantly expanding, but the most intriguing question remains unanswered: are the metabolic enzyme-RNA interactions relevant in the regulation of cell metabolism? It has been proposed that metabolic RNA-binding enzymes participate in general regulatory circuits linking a metabolic function to a regulatory mechanism, similar to the situation of the metabolic enzyme aconitase, which also functions as iron-responsive RNA-binding regulatory element. However, some authors have cautioned that some of such enzymes may merely represent "molecular fossils" of the transition from an RNA to a protein world and that the RNA-binding properties may not have a functional significance. Here I will describe enzymes that have been shown to interact with RNA (in several cases a newly discovered RNA-binding protein has been identified as a well-known metabolic enzyme) and particularly point out those whose ability to interact with RNA seems to have a proven physiological significance. I will also try to depict the molecular switch between an enzyme's metabolic and regulatory functions in cases where such a mechanism has been elucidated. For most of these enzymes relations between their enzymatic functions and RNA metabolism are unclear or seem not to exist. All these enzymes are ancient, as judged by their wide distribution, and participate in fundamental biochemical pathways.
    Źródło:
    Acta Biochimica Polonica; 2006, 53, 1; 11-32
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Characterization and expression analysis of the yellow lupin (Lupinus luteus L.) gene coding for nodule specific proline-rich protein.
    Autorzy:
    Karłowski, Wojciech
    Stróżycki, Paweł
    Legocki, Andrzej
    Powiązania:
    https://bibliotekanauki.pl/articles/1044363.pdf
    Data publikacji:
    2000
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    symbiotic nitrogen fixation
    plant gene expression
    ENOD2
    proline-rich proteins
    early nodulins
    mRNA degradation
    Opis:
    The LlPRP2 gene coding for a proline-rich protein shows a high level of similarity to, as well as significant differences from the family of ENOD2 nodule-specific genes. Several sequence motifs with putative regulatory function were identified in the 5' and 3' noncoding regions of the LlPRP2 gene. Northern blot analysis revealed that the expression of the LlPRP2 gene begins 9 days after inoculation of yellow lupin roots with Bradyrhizobium sp. (Lupinus); the expression is restricted to symbiotic nodules and is not detected in other tissues or organs. Detailed hybridization analysis showed that, when expression is activated, the LlPRP2 transcript is modified so as to produce at least three bands and a continuous distribution of decay intermediates. The modification of the LlPRP2 transcript probably involves degradation from the 5'- and/or 3'-ends of the RNA molecules. Southern blot analysis indicates that only one gene is present in the yellow lupin genome. The presence of genes homologous to the LlPRP2 gene was confirmed for three cultivars of yellow lupin and for Lupinus angustifolius. However, LlPRP2 homologues were not detected in Lupinus albus cv. Bac, indicating that this plant may lack the ENOD2 sequence.
    Źródło:
    Acta Biochimica Polonica; 2000, 47, 2; 371-383
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Tumor necrosis factor-alpha as a possible auto-/paracrine factor affecting estrous cycle in the cat uterus
    Autorzy:
    Siemieniuch, M.J.
    Ogrodowska, K.
    Ohgawara, H.
    Skarzynski, D.J.
    Okuda, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/31521.pdf
    Data publikacji:
    2010
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    oestrous cycle
    cat
    tumour necrosis factor-alpha
    uterus
    prostaglandin
    tumour cell
    cell differentiation
    mRNA
    gene expression
    Opis:
    Tumor Necrosis Factor-alpha (TNFα) is a pleiotrophic cytokine, affects either normal or tumor cells, and influences cellular differentiation. TNFα role in female reproduction has been proven to be mediated through an influence on prostaglandin (PGs) synthesis and output. To evaluate the possible role of TNFα in an auto-/paracrine regulation in the cat uterus, mRNA expression coding for TNFα and its receptors (TNFR1 and TNFR2), and TNFα protein content at different stages of the estrous cycle were investigated. Additionally, TNFα involvement in PG secretion at different stages of the estrous cycle was investigated by in vitro tissue culture. Gene expressions coding for TNFα and TNFR1 were the highest at diestrus (P < 0.05). TNFα protein expression was the lowest at interestrus (P < 0.05). Nevertheless, TNFR2 was not affected by the estrous stage. TNFα at a dose of 1 ng/ml significantly increased PGF₂α secretion at estrus (P < 0.01) and PGE₂ secretion at diestrus (P < 0.001) after 12h incubation. Overall findings indicate that TNFα locally produced in the cat’s uterus, stimulates PG secretion in an estrous cycle-related manner.
    Źródło:
    Polish Journal of Veterinary Sciences; 2010, 13, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Infection with Echinococcus granulosus [Batsch, 1786] and expression of superoxide dismutase gene at mRNA level in hepatocytes
    Autorzy:
    Kozlowska-Loj, J
    Rzymowska, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/839774.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    parasitic disease
    pig
    Echinococcus granulosus
    superoxide dismutase
    infection
    gene expression
    mRNA level
    animal disease
    organism
    parasite
    detoxication
    parasitology
    hepatocyte
    Opis:
    Superoxide dismutase (SOD) plays an important role in detoxication of the organism. Its function is to protect the organism against the cytotoxic action of free radicals. The highest expression of superoxide dismutase was observed in the hepatocytes adjacent to the hydatid cyst. The expression of this gene in the hepatocytes of infected livers 5 cm apart from the infection site was slightly lower.
    Źródło:
    Annals of Parasitology; 2006, 52, 4
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Infection with Echinococcus granulosus [Batsch, 1786] and expression of superoxide dismutase gene at mRNA level in hepatocytes
    Autorzy:
    Kozłowska-Łój, J.
    Rzymowska, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2146352.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    parasitic disease
    pig
    Echinococcus granulosus
    superoxide dismutase
    infection
    gene expression
    mRNA level
    animal disease
    organism
    parasite
    detoxication
    parasitology
    hepatocyte
    Opis:
    Superoxide dismutase (SOD) plays an important role in detoxication of the organism. Its function is to protect the organism against the cytotoxic action of free radicals. The highest expression of superoxide dismutase was observed in the hepatocytes adjacent to the hydatid cyst. The expression of this gene in the hepatocytes of infected livers 5 cm apart from the infection site was slightly lower.
    Źródło:
    Wiadomości Parazytologiczne; 2006, 52, 4; 287-289
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Detekcja ekspresji genów drzew leśnych za pomocą mikromacierzy DNA
    Gene-expression in forest-tree species assessed with microarrays as a tool
    Autorzy:
    Nowakowska, J.A.
    Powiązania:
    https://bibliotekanauki.pl/articles/972859.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Leśne
    Tematy:
    mRNA
    ekspresja genow
    genetyka roslin
    mikromacierze DNA
    sekwencja ESTs
    lesnictwo
    wykrywanie
    drzewa lesne
    microarray
    gene−expression
    forest−tree specie
    ESTs
    Opis:
    DNA microarray technology is a powerful tool in functional genomics study of many organisms, the forest−trees included. This method allow to examine simultaneously the changes in expression of thousands of genes and it is based on specific hybridization of cDNA probes from an organism with the DNA library immobilized in an array. The power of this method consists in miniaturization, automation and parallel study of large−scale genome from multiple samples. In forest science, the microarray technology has already been applied in some study of gene−expression in Populus, Pinus and Picea species and the number of new reports is still increasing every year. Since far, some gene−expression have been studied among woody plants in regard of development and growth processes (xylem, adventious−root and zygotic embryo formation, flowering, ripening, shooting of leaves), resistance mechanisms against biotic (fungi, viruses) and abiotic factors (drought, NaCl, elevated CO2 and O3 concentrations). Many practical applications of the microarray technique may concern the early selection in nursery of trees for morphologically valuable traits, the sustainable forest regeneration and the production of genetically transformed species for the chosen trait.
    Źródło:
    Sylwan; 2006, 150, 04; 33-43
    0039-7660
    Pojawia się w:
    Sylwan
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-9 z 9

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