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    Tytuł:
    Seeding cell number required for optimal lipid accumulation during adipocyte differentiation using 3T3-L1 cell line
    Autorzy:
    Ariyanto, Eko Fuji
    Odaiyappan, Naren A/L S.
    Lidyana, Lynna
    Wikayani, Tenny Putri
    Qomarilla, Nurul
    Wira, Dwi Wahyudha
    Triatin, Rima Destya
    Powiązania:
    https://bibliotekanauki.pl/articles/1076694.pdf
    Data publikacji:
    2019
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    3T3-L1 preadipocytes
    differentiation
    lipid accumulation
    seeding cell number
    Opis:
    Obesity is one of the major causes of metabolic diseases such as diabetes and heart attack, and, hence, can lead to low quality of life. Elaborating adipocyte differentiation is very crucial for formulating the treatment and prevention of obesity. The objective of this study is to investigate the seeding cell number required to obtain optimum lipid accumulation during adipocyte differentiation using the 3T3-L1 cell line. Two sets of 5.48×104 (for Day 0 and Day 8 of differentiation), of 10.96×104 (for Day 8) and of 21.92×104 (for Day 8) of 3T3-L1 cells were seeded in each wells of a 12-well plate. Isobuthylmethylxanthine (IBMX), Dexamethasone, and Insulin-containing differentiation cocktails was added into the medium at Day 0 for 48 hours. The medium was changed every two days. Day 0 and Day 8 samples were then stained using Oil Red O and were examined under the microscope to observe the lipid droplets (red-coloured). The lipid droplets were quantified by measuring the absorbance at wavelength of 550 nm. In the study, seeding the number of 10.96×104 cells produced very significantly higher lipid accumulation, as compared with seeding the number of 5.48×104 cells. However, doubling the seeding number into 21.92×104 cells did not increase the lipid droplets significantly. This study found that the optimum seeding number to obtain the maximum lipid droplets during 3T3-L1 adipocyte differentiation was 10.96×104 cells.
    Źródło:
    World News of Natural Sciences; 2019, 25; 220-226
    2543-5426
    Pojawia się w:
    World News of Natural Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Towards the production of microalgae biofuels: the effect of the culture medium on lipid deposition
    Autorzy:
    Cuellar Garcia, D.J.
    Rangel-Basto, Y.A.
    Barajas-Solano, A.F.
    Munoz-Penalosa, Y.A.
    Urbina-Suarez, N.A.
    Powiązania:
    https://bibliotekanauki.pl/articles/80961.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    biomass production
    biofuel
    microalga
    alga
    Chlorella vulgaris
    Scenedesmus obliquus
    renewable source
    culture medium
    lipid accumulation
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2019, 100, 3
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Physiological response of Brassica napus L. plants to Cu(II) treatment
    Reakcja fizjologiczna Brassica napus L. na działanie miedzi(II)
    Autorzy:
    Peško, M.
    Kráľová, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/126820.pdf
    Data publikacji:
    2013
    Wydawca:
    Towarzystwo Chemii i Inżynierii Ekologicznej
    Tematy:
    akumulacja miedzi
    współczynnik bioakumulacji
    peroksydacja lipidów
    rzepak
    copper accumulation
    bioaccumulation factor
    lipid peroxidation
    rapeseed
    Opis:
    Rośliny rzepaku poddawano działaniu CuSO4 5H2O w siedmiu różnych stężeniach (0,5, 1, 3, 6, 12, 24, 60 μmol · dm–3) przez 7 dni. W zakresie stężeń 0,5-3 μmol·dm–3 zaobserwowano znaczny wzrost biomasy (obie części roślin). Zmniejszenie biomasy zauważono po zastosowaniu wyższych stężeń niż 6 μmol ·dm–3. Znaczny spadek zawartości chlorofili oraz karotenoidów stwierdzono po zastosowaniu 6 μmoli · dm–3 Cu(II). Spadek zawartości białka w liściach roślin zaobserwowano w zakresie stężeń 3-60 μmol · dm–3. Peroksydacja lipidów wyrażona zawartością dialdehydu malonowego w liściach była silna w zakresie stężeń 6-60 μmol · dm–3 Cu(II). Wartości współczynnika bioakumulacji w korzeniach była większe niż w pędach w całym zakresie stężeń (0,5-60 μmol · dm–3 Cu). Stosunek Cu zakumulowanej w pędach do całkowitej ilości miedzi zakumulowanej przez rośliny mieścił się w zakresie od 27,6% (0,5 μmol · dm–3) do 8,4% (60 μmol · dm–3).
    Rapeseed plants were exposed to seven different concentrations (0.5, 1, 3, 6, 12, 24, 60 μmol · dm–3) of CuSO4•5H2O for 7 days. Within concentration range 0.5-3 μmol · dm–3 a significant increase of biomass (both plant organs) was observed. Decrease of biomass was notable after application of concentrations higher than 6 mol · dm–3. Considerable drop in content of chlorophylls as well as carotenoids was observed after application of 6 mol · dm–3 Cu(II). Decline of protein content in leaves of plants was observed in concentration range 6-60 mol · dm–3. Lipid peroxidation expressed as a content of malondialdehyde in leaves was strong within concentration range 6-60 μmol · dm–3 Cu(II). Bioaccumulation factor values of roots were higher then those of shoots in the whole concentration range (0.5-60 μmol · dm–3 Cu). The portion of Cu allocated in shoots related to the total Cu amount accumulated by plant ranged from 27.6% (0.5 μmol · dm–3) to 8.4% (60 μmol · dm–3).
    Źródło:
    Proceedings of ECOpole; 2013, 7, 1; 155-161
    1898-617X
    2084-4557
    Pojawia się w:
    Proceedings of ECOpole
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A comparison of the effects of drought on proline accumulation and peroxidases activity in leaves of Festuca rubra L. and Lolium perenne L.
    Autorzy:
    Bandurska, H
    Jozwiak, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/58679.pdf
    Data publikacji:
    2010
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    red fescue
    grass
    drought
    proline accumulation
    peroxidase
    enzyme activity
    leaf
    Festuca rubra
    Lolium perenne
    lipid peroxidation
    rye-grass
    Opis:
    The effect of soil drought on leaf water content, proline content, and the activity of guaiacol (GuPX) and ascorbate (APX) peroxidases as well as the level of lipid peroxidation were investigated in leaves of drought resistant red fescue (Festuca rubra) and drought sensitive perennial ryegrass (Lolium perenne). Plants were grown under glasshouse conditions in soil pot culture. 26 day-old grasses were exposed to drought by withholding irrigation for 18 days. Water content in leaves of perennial ryegrass decreased more than in red fescue throughout the experimental period. On the other hand, proline content (PC) was higher in red fescue. The activity of APX and GuPX increased in leaves of red fescue, while it did not change in perennial ryegrass. Our data demonstrate that both red fescue and perennial ryegrass were able to survive applied drought, as shown by a lack of stress-induced lipid peroxidation and hence no evidence of oxidative damage. We speculate, that the observed drought stress tolerance at cellular level was associated with the ability to accumulate proline, and to maintain high activity of APX and GuPX, resulting in protection against oxidative damage and lipid peroxidation. It seems that this mechanism works better in red fescue.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2010, 79, 2; 111-116
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Betacyanin accumulation and guaiacol peroxidase activity in Beta vulgaris L. leaves following copper stress
    Autorzy:
    Morales, J.M.L.
    Rodriguez-Monroy, M.
    Sepulveda-Jimenez, G.
    Powiązania:
    https://bibliotekanauki.pl/articles/57381.pdf
    Data publikacji:
    2012
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    betacyanin accumulation
    guaiacol peroxidase activity
    Beta vulgaris
    red beet
    leaf
    copper stress
    oxidative stress
    betacyanin
    abiotic stress
    antioxidant compound
    lipid peroxidation
    copper
    Opis:
    The effect of copper stress on betacyanin accumulation and guaiacol peroxidase (GPOD) activity in leaves of different age was evaluated in red beet (Beta vulgaris L. var. Crosby Egyptian) plants. In hydroponic culture, plants were treated with 0.3 μM (control), 50 μM, 100 μM, and 250 μM of CuSO4 for 6 days. Copper was taken up and accumulated in old roots but was not translocated to leaves. However in young leaves, the increase of lipid peroxidation and reduction of growth were evident from day 3 of copper exposure; whereas in old leaves, the lipid peroxidation and growth were the same from either copper-treated or control plants. In response to copper exposure, the betacyanin accumulation was evident in young leaves by day 3, and continued to increase until day 6. Betacyanin only were accumulated in old leaves until day 6, but the contents were from 4 to 5 times lower than those observed in young leaves at the same copper concentrations. GPOD activity increased 3.3- and 1.4-fold in young and old leaves from day 3 of copper treatment respectively, but only in the young leaves was sustained at the same level until day 6. Old roots shown betacyanin in the control plants, but the betacyanin level and growth were reduced with the copper exposure. In contrast, young roots emerged by copper effect also accumulated copper and showed the highest betacyanin content of all plant parts assayed. These results indicate that betacyanin accumulation and GPOD activity are defense responses to copper stress in actively growing organs.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2012, 81, 3
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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