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Tytuł:
Kidney ultrasound – what is important for a urologist?
USG nerek – co jest istotne dla urologa?
Autorzy:
Szopiński, Tomasz
Keller, Elżbieta
Záťura, František
Powiązania:
https://bibliotekanauki.pl/articles/1051268.pdf
Data publikacji:
2016
Wydawca:
Medical Communications
Tematy:
parenchymal lesions
Opis:
Kidney ultrasound is one of the basic procedures in the practice of a urologist. Apart from the location and the size, description of renal morphology should contain the thickness of the anterior lip parenchyma in a transverse section and the location of possible narrowings. Uneven outline of the kidney is a sign of past inflammatory conditions. In the case of the pelvicalyceal system dilation, it is advised to specify the dimensions of the pelvis and calyces. Convex shape of the calyces proves elevated pressure within the pelvicalyceal system. Hydronephrosis is present when urinary retention has led to thinning the renal parenchyma. In each case, one should identify the reason for urinary retention in the upper urinary tract. Urinary retention on both sides requires one to exclude urinary bladder tumor, it may also be caused by a benign prostatic hyperplasia. Ultrasound examination is a sensitive method of renal stones detection, regardless of their chemical composition. Cyst description in an ultrasound image should cover its morphological features, differentiating between the so-called simple or complex cysts. In the case of a solid lesion, ultrasound makes it possible to detect parenchymal lesions usually starting with the size of 2–2.5 cm. It enables one to particularly diagnose angiomyolipomas. As regards the remaining parenchymal lesions, differentiation of the lesion nature is impossible. In some cases of angiomyolipoma, when it contains bleeding areas present and when it is deficient in adipose tissue, it resembles adenocarcinoma. It is necessary that the description includes the exact location, especially the dimensions and relation of the tumor to the renal sinus. In the case of larger lesions, respiratory motion of the kidney, the condition of the adrenal gland and the presence of enlarged lymph nodes should be controlled. Additionally, one should evaluate the renal vein and inferior vena cava in terms of a neoplastic plug presence.
Badanie ultrasonograficzne nerek jest jednym z podstawowych badań w praktyce urologa. Oprócz położenia i wielkości narządu opis morfologii nerek powinien zawierać grubość miąższu wargi przedniej na przekroju poprzecznym oraz miejsca jego ewentualnych przewężeń. Nierówne zarysy zewnętrzne nerki świadczą o przebytych stanach zapalnych. W przypadku poszerzenia układu kielichowo-miedniczkowego wskazane jest podanie wymiarów miedniczki i kielichów. O podwyższonym ciśnieniu w obrębie układu kielichowo-miedniczkowego świadczy wypukły kształt kielichów. Wodonercze pojawia się wówczas, gdy zastój moczu doprowadził do ścieńczenia miąższu nerki. W każdym przypadku należy zidentyfikować przyczynę zastoju moczu w górnych drogach moczowych. Obustronny zastój moczu wymaga wykluczenia guza pęcherza moczowego, może też wynikać z łagodnego rozrostu stercza. Badanie ultrasonograficzne jest czułą metodą wykrywania złogów, niezależnie od ich składu chemicznego. Opis torbieli w badaniu ultrasonograficznym powinien obejmować jej cechy morfologiczne, różnicujące tzw. torbiele proste oraz złożone. W przypadku zmian litych ultrasonografia pozwala na wykrycie zmian miąższowych zazwyczaj od wielkości 2–2,5 cm. W szczególny sposób pozwala zdiagnozować guzy typu naczyniakomięśniakotłuszczaka. W odniesieniu do pozostałych zmian miąższowych nie jest możliwe zróżnicowanie charakteru zmiany. W niektórych przypadkach naczyniakomięśniakotłuszczak, gdy zawiera obszary krwawień oraz gdy jest ubogi w tkankę tłuszczową, upodabnia się do gruczolakoraka. W opisie konieczne jest uwzględnienie dokładnej lokalizacji, zwłaszcza wymiarów i stosunku guza do zatoki nerkowej. W przypadku większych zmian należy skontrolować ruchomość oddechową nerki, stan nadnercza i obecność powiększonych węzłów chłonnych. Dodatkowo powinno się ocenić żyłę nerkową i główną dolną pod kątem obecności czopa nowotworowego.
Źródło:
Journal of Ultrasonography; 2016, 16, 67; 371-377
2451-070X
Pojawia się w:
Journal of Ultrasonography
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of antlerogenic stem cells on the healing of lesions in the corneal epithelium and corneal stroma in rabbits
Autorzy:
Kiełbowicz, M.
Kuropka, P.
Cegielski, M.
Kiełbowicz, Z.
Trębacz, P.
Hebel, M.
Aleksiewicz, R.
Powiązania:
https://bibliotekanauki.pl/articles/2087321.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
eye
stem cells
cornea
rabbit
healing
lesions
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 2; 281-290
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Navigation with Use of Intra-Operative Ultrasound in Search for Neoplastic Lesions of Endocrine Glands
Autorzy:
Sopiński, Jan
Kuzdak, Krzysztof
Powiązania:
https://bibliotekanauki.pl/articles/1396374.pdf
Data publikacji:
2013-05-01
Wydawca:
Index Copernicus International
Tematy:
intraoperative ultrasound
neoplastic lesions
endocrine glands
Opis:
was to evaluate the effectiveness of intraoperative ultrasonography (IOUS) during operations of endocrine glands tumors. Material and methods. The study was conducted in patients who underwent endocrine operation in Department of Endocrine, General and Vascular Surgery, Medical University in Łódź in 2008-2011. Results. Patients with thyroid cancer recurrences:in study group we managed shorter lesion access time (10 ± 4.47 min vs 16.78 ± 8.9 min; p=0.04). Time of surgery was also shorter in study group (75 ± 30.17 minvs 85,71 ± 38.92 min), but it was not significant (p=0.46). The use of IOUS did not affect the hospitalization time (2.91 ± 1.64 days vs 3 ± 1.66 days; p=0.820), intraoperative blood loss (45.45 ± 105.96 ml vs 40 ± 82.89 ml; p=0.972) and the rate of intraoperative complications (1/11 - 9.09% vs 2/14 - 14.29%; p=1). Patients with primary hyperparathyroidism: the time of surgery (58± 22.74 min vs 65 ± 19.6 min; p=0.336) and the lesion access time (13.33 ± 7,94 min vs 17.25 ± 8.19 min; p=0.169) were shorter in study group. Hospitalization time was longer in study group (6.13 ± 5.3 days vs 4.45 ± 4.58 days; p=0.079). The rate of intraoperative complications was higher in study group (3/15 - 20% vs 2/20 - 10%; p=0.631). None of this results were statistically significant (p≤0.05). Patients who underwent open adrenalectomy: in study group we managed significantly shorter time of surgery (70 ± 44.35 min vs 80.12 ± 29.60 min; p=0.033) and shorter lesion access time (12 ± 8.88 min vs 17.37 ± 7.42 min; p=0.045). The use of IOUS did not affect the hospitalization time (5.6 ± 1.65 days vs 6.35 ± 2.38 days; p=0.429), intraoperative blood loss (110 ± 164.65 ml vs 172.5 ± 226.35 ml; p=0.442) and rate of intraoperative complications (0/10 vs 1/40; p=1). Patients who underwent videoscopicadrenalectomy: in study group we managed to get shortertime of surgery (89.44 ± 27.11 min vs 109.12 ± 33.88 min; p=0.034) and shorter lesion access time (28.61 ± 14.93 min vs 45.98 ± 20.44 min; p=0.002). Intraoperative blood loss was also significantly lower in study group (86.11 ± 157 ml vs 169.27 ± 201.04 ml; p=0.037). The use of IOUS did not affect the hospitalization time (4.39 ± 3.27 days vs 3.83 ± 3.67 days; p=0.227), the rate of intraoperative complications (0/18 vs. 2/41; p=1) and the conversion rate (2/20-10% vs. 5/46- 10.87%; p=1). Conclusions. 1.During adrenalectomies this technique facilitates finding the pathological lesion shortening the time of access to the tumor and procedure duration. 2. IOUS is useful for determining the tumor relationship with the surrounding anatomical structures. 3. IOUS isa useful technique in the assessment of adrenal tumor infiltration of vena cava. 4. The use of IOUS allows the surgeon to assess anatomical relationships in the real time, after incision and retraction of tissues. 5. During operations of thyroid cancer recurrences using this technique makes easier to find a lesion in the operated area and it is possible to asses radical of surgery. 6. The use of IOUS allows to find pathological parathyroid glands inside thyroid gland. 7. IOUS is useful in the detection of thyroid pathology during parathyroidectomy.
Źródło:
Polish Journal of Surgery; 2013, 85, 5; 262-270
0032-373X
2299-2847
Pojawia się w:
Polish Journal of Surgery
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The usefulness of the NBI – narrow band imaging for the larynx assessment
Autorzy:
Kimza, Hanna
Jackowska, Joanna
Wierzbicka, Małgorzata
Powiązania:
https://bibliotekanauki.pl/articles/1398132.pdf
Data publikacji:
2018
Wydawca:
Index Copernicus International
Tematy:
NBI
phonosurgery
vocal fold benign lesions
laryngeal papillomatosis
Opis:
Narrow band imaging (NBI) by enhancing the contrast between the mucosal epithelium and submucosal vessels facilitates diagnosis of precancerous and cancerous lesions, as well as hypertrophic lesions such as laryngeal papillomatosis. Narrow band imaging (NBI) is an optical technique based on the modification of white light by the use of special optical filters. Every change in the microvascular architecture of the mucosa is classified according to Ni’s classification (2011). The use of NBI improves sensitivity and specificity of assessment of laryngeal lesions and allows more precise assessment of the status of surgical margins of early-stage and locally-advanced laryngeal cancers managed in endoscopic laser cordectomy.
Źródło:
Polish Journal of Otolaryngology; 2018, 72, 3; 1-3
0030-6657
2300-8423
Pojawia się w:
Polish Journal of Otolaryngology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Skin and mucosal lesions in otolaryngological practice
Autorzy:
Sobolewski, Piotr
Ziuzia, Laura
Dobrzyński, Paweł
Walecka, Irena
Powiązania:
https://bibliotekanauki.pl/articles/1400416.pdf
Data publikacji:
2017
Wydawca:
Index Copernicus International
Tematy:
otolaryngology
dermatology
skin lesions
mucosal lesions
pemphigus
pemphigoid
Behçet's disease
lichen planus
Stevens - Johnson syndrome
Lyell's syndrome
Opis:
Cutaneous and mucosal lesions are seen in many diseases, frequently being the main element of the clinical presentation. It is not uncommon that the patient with mucosal lesions of the upper respiratory tract consults the otolaryngologist first, therefore it is important that those specialists know the mucosal symptoms of dermatological diseases and consider them in the differential diagnosis. In this article diseases with the mucosal lesions of the upper respiratory tract are described, including pemphigus, pemphigoid, lichen planus, and Behçet’s disease. Knowledge of the morphology of these mucosal manifestations is essential in the clinical practice of both dermatologists and otolaryngologists, and the diagnostic and therapeutic success may be achieved only with the cooperation of these specialists.
Źródło:
Polski Przegląd Otorynolaryngologiczny; 2017, 6, 3; 34-40
2084-5308
2300-7338
Pojawia się w:
Polski Przegląd Otorynolaryngologiczny
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prevalence and major causes of ruminal lesions in intensive cattle fattening units
Autorzy:
Vieira, J.
Matos, R.
Cannas da Silva, J.
Baumgartner, W.
Van Harten, S.
Dâmaso, A.
Powiązania:
https://bibliotekanauki.pl/articles/16647393.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
ruminal lesions
ruminal acidosis
cattle
feedlot
management
Opis:
Ruminal acidosis is frequent in cattle fattening systems associated with grain-rich fibre-poor diets. This study aimed to assess the prevalence of ruminal lesions compatible with ruminal acidosis intensive fattening farms and its risk factors on farm. Rumens of 218 out of 1960 bullocks from six farms were classified in slaughterhouses, and the management practices and the type of feed were compared. The prevalence of lesions compatible with ruminal acidosis was high (65%), where 47% and 18% of the animals had ruminal lesions compatible with subacute and acute disease, respectively. Prevalence of lesions differed significantly between the farms, with differences in the management and feeding practices. Ruminal lesions compatible with ruminal acidosis are prevalent in cattle fattening units in Portugal and husbandry and feeding practices are likely to have an impact.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 4; 635-637
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genotype-specific human papillomavirus detection in cervical smears
Autorzy:
Szostek, Slawa
Klimek, Malgorzata
Zawilinska, Barbara
Kosz-Vnenchak, Magdalena
Powiązania:
https://bibliotekanauki.pl/articles/1040671.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
human papillomavirus
cervical carcinoma
HPV genotyping
squamous intraepithelial lesions
Opis:
Human papillomavirus (HPV) is widely accepted as a causative agent of cervical cancer. The distribution and prevalence of HPV types depend on geographic region and demographic factors. The aim of this study was to investigate the relationship between the presence of various HPV types and the outcome of cytological examination. Cervical smears were obtained from 125 women from southern Poland: low grade squamous intraepithelial lesions (LSIL) - 44, high grade squamous intraepithelial lesions (HSIL) - 12, cervical carcinoma - 27 and 42 women without abnormality in cytology as a control group. DNA was extracted from the smears and broad-spectrum HPV DNA amplification and genotyping was performed with the SPF 10 primer set and reverse hybridisation line probe assay (INNO-LiPA HPV Genotyping, Innogenetics). HPV DNA was detected in approximately 72% cases, more frequently in women with squamous intraepithelial lesions and cervical carcinoma than in the control group (P < 0.0005). The most frequent type found was HPV 16 (37%), followed by HPV 51 (28%) and HPV 52 (17%). A single HPV type was detected in 51% positive cases, more frequently in cervical cancer specimens. Multiple HPV infection was dominant in women with LSIL and normal cytology. Prevalence of HPV 16 increased with the severity of cervical smear abnormality. For women HPV 16 positive, the relative risk (odds ratio) of the occurrence of HSIL and cervical cancer versus LSIL was 14.4 (95% CI, 3.0-69.2; P=0.001) and 49.4 (95% CI, 6.5-372.8; P < 0.001), respectively. Genotyping of HPV will allow better classification of women with cervical abnormalities into different risk groups and could be useful in therapy.
Źródło:
Acta Biochimica Polonica; 2008, 55, 4; 687-692
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Herpesviruses as possible cofactors in HPV-16-related oncogenesis*
Autorzy:
Szostek, Slawa
Zawilinska, Barbara
Kopec, Jolanta
Kosz-Vnenchak, Magdalena
Powiązania:
https://bibliotekanauki.pl/articles/1040594.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
human papillomavirus
cervical carcinoma
squamous intraepithelial lesions
herpesviruses
Opis:
Cervical carcinogenesis is a complex problem with papillomavirus widely accepted as a causative agent. Integration of a human papillomavirus (HPV) of the high-risk type into the host cell genome is one of the major contributing factors to cervical malignant transformation. In this study, the correlation of CMV, EBV, HSV-1, HSV-2, HHV-6 and HHV-7 infections with the physical status of the HPV genome in cervical cancer and precancerous cervical lesions was investigated in sixty HPV-16-positive women. Cervical secretion samples were submitted to DNA extraction and analyzed by PCR. HPV-16 DNA was confirmed in genotyping with the reverse hybridization line probe assay. Multiplex PCR with specific primers for the E2/E6 genes was used to assess the viral integration status of HPV-16. Our results show that CMV DNA was more frequently present in samples with mixed forms of HPV-16 than in the episomal form (P < 0.025). Such a correlation was also observed in the case of EBV (P < 0.005). The presence of CMV resulted in a six-fold (OR 6.069; 95% CI 1.91-19.22; P = 0.002), while EBV caused a seven-fold (OR 7.11; 95% CI 1.70-29.67; P = 0.007) increase in the risk of the integrated or mixed HPV-16 genome occurrence. Our data suggest that coinfection with herpesviruses, especially CMV and EBV, may be involved in the integration of the HPV-16 genome and may contribute to the development of cervical cancer.
Źródło:
Acta Biochimica Polonica; 2009, 56, 2; 337-342
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Mammotome biopsy under ultrasound conrol in the diagnostics and treatment of nodular breast lesions – own experience
Autorzy:
Kibil, Wojciech
Hodorowicz-Zalewska, Diana
Kulig, Jan
Powiązania:
https://bibliotekanauki.pl/articles/1394597.pdf
Data publikacji:
2012
Wydawca:
Index Copernicus International
Tematy:
mammotome biospy
ultrasound-guided biopsy
nodular breast lesions
Opis:
Mammotome biopsy is an effective, minimally invasive, novel technique used in the verification of breast lesions. The aim of the study was to assess the value of ultrasound-guided vacuum-assisted core needle biopsy (mammotome biopsy) in the diagnostics and treatment of nodular breast lesions, considering own data. Material and methods. Analysis comprised 1183 mammotome biopsies under ultrasound control performed in 1177 female patients during the period between 2000 and 2010, at the Regional Clinic for Early Diagnostics and Treatment of Breast Lesions, I Chair and Department of General Surgery, Jagiellonian University, Collegium Medicum. Results. The average patient age amounted to 41.7 years. The size of the investigated lesions ranged between 4 and 65 mm (mean – 12 mm). The histopathological examination result was as follows: fibrocystic lesions (n=285), adenofibroma (n=477), adenosis sclerosans (n=188), hyperplasia without atypy (n=58), phyllode tumor (n=2), papilloma (n=14), hamartoma (n=1), atypical hyperplasia (n=25), in situ ductal carcinoma (n=4), in situ lobular carcinoma (n=5), infiltrating ductal carcinoma (n=114), infiltrating lobular carcinoma (n=4), non-diagnostic result (n=6). The histopathological diagnosis was obtained in 99.5% of cases. Patients diagnosed with atypical hyperplasia or cancer were qualified for surgery, according to accepted standards. The presence of a hematoma was the most common complication after the biopsy, observed in 16.5% of patients. Conclusions. The obtained results confirmed the high value of ultrasound-guided biopsies in the diagnostics of nodular breast lesions. The method is safe, minimally invasive, with few complications, providing a good cosmetic effect. In case of benign lesions with a diameter of less than 15 mm the mammotome biopsy enables to completely excise the lesions, being an alternative to open surgical biopsies. The mammotome biopsy should become the method of choice considering the diagnostics of nodular breast lesions.
Źródło:
Polish Journal of Surgery; 2012, 84, 5; 242-246
0032-373X
2299-2847
Pojawia się w:
Polish Journal of Surgery
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The predictive value of videostroboscopy in the assessment of premalignant lesions and early glottis cancers
Autorzy:
Rzepakowska, Anna
Sielska-Badurek, Ewelina
Osuch-Wójcikiewicz, Ewa
Sobol, Maria
Niemczyk, Kazimierz
Powiązania:
https://bibliotekanauki.pl/articles/1398516.pdf
Data publikacji:
2017
Wydawca:
Index Copernicus International
Tematy:
laryngovideostroboscopy
precancerous lesions
larynx
glottis
early glottic cancer
Opis:
Objective: To assess the sensitivity and specificity of larngovideostroboscopy (LVS) in the diagnosis of precancerous and malignant lesions of the vocal folds. Material and methods: In 175 patients (128 men and 47 women), aged 19-88 years, mean age 61.5, who were admitted to the clinic with diagnosed premalignant conditions of vocal fold mucosa (leukoplakia, chronic hypertrophic inflammatory lesions) and thickening or tumor on the vocal fold, there was performed LVS before the laryngeal microsurgery. The LVS study included: localization of the leasion, movement of the vocal folds, mucosal wave, shape of glottis clousure, amplitude and symmetry of vocal fold vibration. In the evaluation, a point scale was applied for the individual functional parameters. The scale ranged from 0 to 14. Patients with impaired vocal fold motion or absent mucosal wave were positive on LVS for malignant lesions. Those with limitted mucosal wave were positive on LVS for dysplastic lesions. The results were compared with the final histopathological examination and the sensitivity, specificity, accuracy, positive (PPV) and negative (NPV) predictive value were calculated. Results: On the basis of histopathological examination, benign lesions (normal or inflammatory mucosa) accounted for 20% of diagnoses, hypertrophy and parakeratosis for 28%, low and middle grade dysplasia accounted for 10% and malignant lesions (high-grade dysplasia, pre-invasive cancer, Invasive cancer) was diagnosed in 42% of patients. The overall mean score for LVS was 4.5 and 8.0, respectively for benign and malignant lesions. Sensitivity, specificity, accuracy, PPV and NPV of LVS in detecting malignant lesions were respectively - 95.6%, 23.8%, 61.1%, 57.6% and 83.3% and in detecting both premalignant and malignant lesions were respectively – 90.7%, 31.4%, 78.9%, 84.1% and 45.8%. Conclusions: Because of the high sensitivity of LVS in detecting precancerous and malignant lesions, this method is a very good tool for screening of pathology within the larynx.
Źródło:
Polish Journal of Otolaryngology; 2017, 71, 4; 14-18
0030-6657
2300-8423
Pojawia się w:
Polish Journal of Otolaryngology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Haemoglobin scavenger receptor: function in relation to disease
Autorzy:
Zuwała-Jagiełło, Jolanta
Powiązania:
https://bibliotekanauki.pl/articles/1041233.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
inflammation
atherosclerotic lesions
haem oxygenase
haemoglobin scavenger receptor
CD163
Opis:
Highly efficient systems remove the toxic and proinflammatory haemoglobin from the circulation and local sites of tissue damage. Macrophages are major haemoglobin-clearing cells; CD163 was recently recognized as the specific haemoglobin scavenger receptor (HbSR). It is tightly involved in both physiological as well as pathophysiological processes, such as cytoprotection and inflammation. Haemoglobin functions as a double-edged sword. In moderate quantities and bound to haptoglobin, it forms a ligand for haemoglobin scavenger receptor CD163/HbSR, but when unleashed in large amounts, it can become toxic by mediating oxidative stress and inflammation. CD163/HbSR plays a crucial role in the control of inflammatory processes, probably in part through its effects on both ferritin induction and subsequent induction of antiinflammatory pathways through interleukin-10 and haem oxygenase. Besides the observation that the haemoglobin scavenger receptor provides a promising target for new treatment possibilities, it offers a novel view on the aetiology of diverse physiological as well as pathophysiological processes. In addition, monocyte CD163/HbSR and soluble CD163/HbSR are potential diagnostic tools in a variety of disease states, such as inflammation, atherosclerosis, transplant rejection, and carcinoma.
Źródło:
Acta Biochimica Polonica; 2006, 53, 2; 257-268
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Physical state of human papillomavirus type 16 in cervical intraepithelial lesions and cancers determined by two different quantitative real-time PCR methods
Autorzy:
Szostek, Slawa
Biesaga, Beata
Zawilinska, Barbara
Klimek, Malgorzata
Kosz-Vnenchak, Magdalena
Powiązania:
https://bibliotekanauki.pl/articles/1038944.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
real-time PCR
human papillomavirus
squamous intraepithelial lesions
cervical carcinoma
Opis:
The aim of this study was to analyse the correlation between a new multiplex qPCR assay and a reference qPCR assay for assessment of the human papillomavirus (HPV16) load and the viral genome status. The study was performed on 100 HPV16 positive samples containing premalignant lesions and carcinomas. HPV16 E2 and E6 gene loads were assessed by two PCR methods. The load of E2 and E6 was normalized to the cell number by qPCR targeting the RNase P open reading frame. The physical state of the viral genome was determined as a ratio of E2/E6 copies number per cell. Among 100 samples analysed, there were no statistically significant differences in the E2 and E6 viral load evaluated by multiplex qPCR and qPCR, the correlation coefficients were 0.98 and 0.97, respectively. There were 19% of samples with the integrated, 73% with mixed and 8% with episomal state of viral genome detected by multiplex qPCR and 17%, 79%, 4%, respectively, found by qPCR. Prevalence of integrated and episomal forms estimated by multiplex qPCR was higher than the one obtained by qPCR (Chi2, p < 0.0001), but in samples with premalignant and malignant diagnoses no significant differences were demonstrated regardless of the methods used. Sensitivity and specificity of multiplex qPCR were 93.7% and 100% as compared with qPCR, the positive predictive value was 100%. In summary, the multiplex qPCR assay in respect of HPV16 load and the frequency of viral genome status was shown to be a sensitive and specific reference method. Simultaneous estimation of E2 and E6 genes in one reaction tube reduces the cost of testing.
Źródło:
Acta Biochimica Polonica; 2015, 62, 4; 923-928
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Efficiency of selected physiotherapeutic treatments for low back pain
Autorzy:
Dakowicz, A.
Milewska, A. J.
Gradkowska, A.
Matys, A.
Tarkowska, K.
Białowieżec, M.
Powiązania:
https://bibliotekanauki.pl/articles/1918383.pdf
Data publikacji:
2016
Wydawca:
Uniwersytet Medyczny w Białymstoku
Tematy:
Low back pain
degenerative lesions
physiotherapy
kinesiotherapy
physical therapy
Opis:
Purpose: Low back pain (LBP) occurs among approximately 60-80% of the population, gradually contributing to long-term or recurring disability. We aimed to evaluate the efficiency of two physiotherapeutic treatments (namely, Träbert current and kinesiotherapy) among patients with LBP. Material and methods: Comprised of function tests and scales of pain and functionality, clinical tests were performed according to a prospective method in a regional hospital in Lomza. Forty patients randomly distributed into two groups: one group a subject to physical therapy with Träbert currents, the other group to kinesiotherapy following a proprietary programme. Physiotherapy was performed from Monday to Friday for two weeks in a series of ten treatments. Results: The treatment used in both groups significantly decreased pain and increased functionality of patients, evaluated by Laitinen’s survey, the Oswestry Disability Index (ODI), and the Roland Morris Disability Questionnaire. Results of the Schober’s and “fingertip-to-floor” tests also showed increased improvement. Conclusions: The physiotherapy facilitated a significant decrease in pain, an increase in the mobility in lumbosacral segment of the spine and a decrease in disability among patients with LBP.
Źródło:
Progress in Health Sciences; 2016, 6(2); 70-76
2083-1617
Pojawia się w:
Progress in Health Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Myositis Ossificans of the Quadriceps Femoris Mimicking Sarcoma as a Diagnostic and Therapeutic Problem – Case Report and Literature Review
Autorzy:
Nowaczyk, Piotr
Murawa, Dawid
Żmudzińska, Magdalena
Wasiewicz, Janusz
Powiązania:
https://bibliotekanauki.pl/articles/1396375.pdf
Data publikacji:
2013-09-01
Wydawca:
Index Copernicus International
Tematy:
myositis ossificans
heterotopic ossification
sarcomas
pseudosarcomas
post-traumatic lesions
Opis:
Myositis ossificans (MO) may be included in the group of lesions described as pseudosarcomas. Its clinical and histological picture frequently mimics a malignant neoplasm and therefore, ultimate diagnosis and implementation of adequate treatment requires the cooperation of interdisciplinary team of physicians. The paper presents the case of 20-year old female patient suffering from severe pain in the right thigh. The patient was initially diagnosed with the lower limb overload. Rest and administration of non-steroidal anti-inflammatory drugs (NSAID) were recommended. Due to the lack of the efficacy of the recommended conservative treatment and detection of tumorous mass on ultrasound examination, the patient was referred to the cancer centre. The diagnostic procedures were extended and an open biopsy of the lesion was performed which revealed the presence of MO. The patient underwent a surgical procedure during which the pathological mass was entirely removed. Follow up examinations conducted upon the conclusion of the rehabilitation indicate no pathologies in the operated area.
Źródło:
Polish Journal of Surgery; 2013, 85, 9; 520-526
0032-373X
2299-2847
Pojawia się w:
Polish Journal of Surgery
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Insect bites as a trigger factor of eosinophilic cellulitis
Autorzy:
Kucharczyk, M.
Slowik-Rylska, M.
Krecisz, B.
Powiązania:
https://bibliotekanauki.pl/articles/2083114.pdf
Data publikacji:
2019
Wydawca:
Instytut Medycyny Wsi
Tematy:
eosinophilic cellulitis
Wells Syndrome
flame figures
morphea-like lesions
Opis:
Introduction. The study presents the case report of a Wells Syndrome in a 18-year -ld female. Wells Syndrome is a rare inflammatory skin disorder which seems to present an abnormal eosinophilic response to a number of triggers. Objective. The aim of the study is to discuss several problems related to the diagnosis and treatment of Wells Syndrome. Materials and method. Medical examination, blood tests, abdominal ultrasound and skin biopsy were performed. Results. Medical examination revealed plaques with tense blisters on feet and erythematous lesions on trunk. Blood tests showed raised anti-streptolysin O level. Skin biopsy revealed features of eosinophilic cellulitis. Oral administration of 0.5 mg per day prednisolone and antihistaminics with local therapy resulted in good clinical response. Conclusions. Sometimes several biopsy are required to establish diagnosis. In some cases lower doses of corticosteroids with antihistaminics and local anti-inflammatory treatment can by sufficient in treatment.
Źródło:
Annals of Agricultural and Environmental Medicine; 2019, 26, 2; 256-259
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł

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