- Tytuł:
- SSCP polymorphism within 5 region of bovine lactoglobulin [LGB] gene
- Autorzy:
-
Kaminski, S
Zabolewicz, T. - Powiązania:
- https://bibliotekanauki.pl/articles/2044462.pdf
- Data publikacji:
- 1998
- Wydawca:
- Polska Akademia Nauk. Czytelnia Czasopism PAN
- Tematy:
-
lactoglobulin gene
polymorphism
SSCP method
cattle
gene transcription
electrophoresis
hormonal receptor
mutation
beta-lactoglobulin
milk - Opis:
- In the paper the detection of the SSCP polymorphism within the 5’ fragment of bovine beta-lactoglobulin (LGB) gene is described. The 5’ fragment of LGB gene (209 bp) was PCR-amplified and then subjected to electrophoresis allowing the detection of SSCP polymorphism. Among 124 animals (50 cows and 74 bulls) six SSCP patterns were identified and named Rl, R2, R3, R4, R5 and R6, which occured with the frequency of 0.32, 0.51, 0.09, 0.06, 0.01 and 0.01, respectively. The PCR-SSCP method is simple, fast, and relatively inexpensive. The SSCP polymorphism reported in the paper may be useful in looking for the associations between different SSCP patterns and LGB gene expression and milk properties.
- Źródło:
-
Journal of Applied Genetics; 1998, 39, 1; 97-102
1234-1983 - Pojawia się w:
- Journal of Applied Genetics
- Dostawca treści:
- Biblioteka Nauki
Artykuł