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Tytuł:
Determination of lanthanum(III) and europium(III) – lactate interaction constants by capillary electrophoresis for environmental applications
Autorzy:
Bortolus, S.
Varenne, A.
Draye, M.
Gareil, P.
Czerwiński, K.
Cote, G.
Powiązania:
https://bibliotekanauki.pl/articles/347001.pdf
Data publikacji:
2003
Wydawca:
Politechnika Bydgoska im. Jana i Jędrzeja Śniadeckich. Wydział Technologii i Inżynierii Chemicznej
Tematy:
lanthanides(III)
lactate
separation
interaction constants
capillary electrophoresis
Opis:
In the present work, we investigated the ability of electrophoresis to determine the stability constant of the complex formed between lactate ions (a simple model of natural organic matter), and lanthanum (III) and europium (III), two model metal cations of actinides. The effect of lactate on lanthanide’s effective mobility is studied, and conditional metal ion-ligand interaction constants are determined on the basis of the variation in the effective mobilities (log βn = 2.23 for lanthanum (III) and 2.39 for europium (III), at 25°C and I=0.015 M). The originality of this contribution lies in the fact that we have chosen to detect the metal ion instead of the ligand by an indirect UV-visible detection and using a chromophoric compound (creatinine).
Źródło:
Ars Separatoria Acta; 2003, 2; 29-35
1731-6340
Pojawia się w:
Ars Separatoria Acta
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effects of warm-up duration on cycling time trial performance in trained cyclists
Autorzy:
Bunn, Jennifer A.
Eschbach, L. Chris
Magal, Meir
Wells, Elizabeth K.
Powiązania:
https://bibliotekanauki.pl/articles/1054805.pdf
Data publikacji:
2017
Wydawca:
Uniwersytet Szczeciński. Wydawnictwo Naukowe Uniwersytetu Szczecińskiego
Tematy:
Maximal oxygen consumption
aerobic
endurance training
lactate
oxygen kinetics
Opis:
The purpose of this study was to assess the effects of three different warm-up condi-tions on a 5K cycling time trial (TT). Sixteen trained cyclists completed the study. At the first testing session, participants completed a maximal graded exercise test to assess maximal oxygen consumption (VO2max) and a familiarization of the TT. At three subse-quent visits, the participants completed the TT after no warm up, short warm-up of three minutes at 60% VO2max, or long warm-up of ten minutes at 60% VO2max. The warm-up was assigned in randomized order. VO2, heart rate (HR), lactate, power, and speed were assessed after the warm-up, 1K, and completion of the 5K TT. There was no dif-ference between type of warm-up for time, power, cadence, speed, VO2, HR, or lactate levels at the end of the TT. There was no significant difference between type of warm-up for time, VO2 or HR at the end of the 1K split. Warm-up length was not impactful on 5K TT performance or during the first km of the TT in trained cyclists. These results con-flict with previous evidence indicating that a warm-up in endurance events primarily improved VO2 kinetics at the onset of the exercise.
Źródło:
Central European Journal of Sport Sciences and Medicine; 2017, 17, 1; 5-13
2300-9705
2353-2807
Pojawia się w:
Central European Journal of Sport Sciences and Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Group II intron-mediated deletion of lactate dehydrogenase gene in an isolated 1,3-propanediol producer Hafnia alvei AD27
Autorzy:
Celińska, Ewelina
Drożdżyńska, Agnieszka
Wita, Agnieszka
Juzwa, Wojciech
Białas, Wojciech
Czaczyk, Katarzyna
Grajek, Włodzimierz
Powiązania:
https://bibliotekanauki.pl/articles/1038697.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
1,3-propanediol
gene knock-out
metabolic engineering
Enterobacteriaceae
group II intron
lactate dehydrogenase
Opis:
Our previous studies showed that glycerol fermentation by Hafnia alvei AD27 strain was accompanied by formation of high quantities of lactate. The ultimate aim of this work was the elimination of excessive lactate production in the 1,3-propanediol producer cultures. Group II intron-mediated deletion of ldh (lactate dehydrogenase) gene in an environmental isolate of H. alvei AD27 strain was conducted. The effect of the Δldh genotype in H. alvei AD27 strain varied depending on the culture medium applied. Under lower initial glycerol concentration (20 gL-1), lactate and 1,3-propanediol production was fully abolished, and the main carbon flux was directed to ethanol synthesis. On the other hand, at higher initial glycerol concentrations (40 gL-1), 1,3-propanediol and lactate production was recovered in the recombinant strain. The final titers of 1,3-propanediol and ethanol were similar for the recombinant and the WT strains, while the Δldh genotype displayed significantly decreased lactate titer. The by-products profile was altered upon ldh gene deletion, while glycerol utilization and biomass accumulation remained unaltered. As indicated by flow-cytometry analyses, the internal pH was not different for the WT and the recombinant Δldh strains over the culture duration, however, the WT strain was characterized by higher redox potential.
Źródło:
Acta Biochimica Polonica; 2017, 64, 1; 123-133
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of Water Treatment and Immersion in Calcium Salt Solutions on the Quality of Fruits of Peumo Pink Tomato (Solanum lycopersicum L.) Stored under Cold Conditions
Autorzy:
Correa, Julio
Amoros, Asuncion
Silva, Herman P.
Covarrubias, José I.
Escalona, Victor H.
Powiązania:
https://bibliotekanauki.pl/articles/2051046.pdf
Data publikacji:
2022-06-09
Wydawca:
Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
Tematy:
calcium chloride
calcium lactate
calcium propionate
postharvest
heat treatment
heat shock protein
Opis:
Tomato is one of the most consumed vegetable crops worldwide. In the break stage, it is perishable, and it has a postharvest life that does not exceed three weeks at 12 to 15°C. The aim of this study was to evaluate the effect of immersion in water and in calcium salt solutions on the metabolism and quality of tomato of traditional Peumo Pink cultivar stored for 28 days at 10ºC plus a simulated trading period of 2 days at 20ºC. Fruits were treated in water at 5, 45 and 60ºC at two immersion times of 1 and 4 min or in 2% solutions of calcium chloride, lactate and propionate at 10 and 45ºC for 4 min. The respiration rate, ethylene production and heat shock protein gene expression as well as firmness, total phenolic content and antioxidant capacity of tomato were determined. Dipping in water at 60°C reduced the loss of firmness and respiratory rate of tomatoes up to 21 days at 10°C + 2 days at 20°C. Treatment in water at 45°C for 4 min and at 60°C for 1 or 4 min stimulated heat shock protein gene expression. However, fruit treated at 60°C for 1 or 4 min showed uneven ripening; hence, the immersion in water at 45°C for 4 min was the most recommended to extend the postharvest life of tomatoes. In turn, the immersion in calcium lactate and propionate solutions at 45°C increased total calcium content and maintained firmness after 28 days at 10°C + 2 days at 20°C. The use of high temperature and calcium salts for dipping would be beneficial to provide the vegetal tissues with calcium and reduce the softening of the tomato after prolonged cold storage.
Źródło:
Polish Journal of Food and Nutrition Sciences; 2022, 72, 2; 193-202
1230-0322
2083-6007
Pojawia się w:
Polish Journal of Food and Nutrition Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Rapid determination of lactic acid in anaerobic biological treatment process using a portable sensitive lactate biosensor
Autorzy:
Darwin, D.
Powiązania:
https://bibliotekanauki.pl/articles/80265.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
rapid determination
lactic acid
lactate concentration
lactate biosensor
anaerobic digestion
acidification
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2019, 100, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparative interpretation of lactate measurement by point of care spectrophotometric and ELISA methodsin transition cows
Autorzy:
Erdoğan, H.
Ünübol Aypak, S.
Erdoğan, S.
Ural, K.
Powiązania:
https://bibliotekanauki.pl/articles/2087619.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
accuracy
bovine
portable lactate meter
transition period
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 4; 741-746
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
New microfluidic device for lactate dehydrogenase (LDH) activity analysis
Autorzy:
Jędrych, E.
Mazur, M.
Grabowska-Jadach, I.
Brzózka, Z.
Powiązania:
https://bibliotekanauki.pl/articles/115778.pdf
Data publikacji:
2012
Wydawca:
Fundacja na Rzecz Młodych Naukowców
Tematy:
lactate dehydrogenase (LDH) activity
microfluidic system
PDMS
adherent cell culture
cytotoxicity analysis
Opis:
In this paper, we present cytotoxicity analysis (determination of lactate dehydrogenase — LDH activity performed in a designed and fabricated microfl uidic system. This method allowed for analysis of a supernatant collected from A549 (human lung cancer) and HT-29 (human colon cancer epithelial) cells, which were incubated for 24 h with selected compounds. LDH is an intracellular enzyme present in tissues, which is released into the supernatant caused by membrane damage or cell lyses. In our tests, LDH-Cytotoxicity Assay Kit (BioVision) was used. The toxic eff ect of drugs was measured in the developed microsystem made of PDMS (poly(dimethylsiloxane)). Analytical reaction took place in the special designed microchannel geometry. Then, the LDH activity was measured at 490 nm using spectrophotometer. In subsequent experiments, appropriate conditions for measurements using a microfl uidic system were chosen. It was found that the selected reagent is sensitive to temperature changes and light exposure. Reaction time in the microsystem was determined by changes of fl ow rates of reagents. Afterwards, for the various reaction time, the toxic eff ect of 5-fl uorouracil, celecoxib and 1,4-dioxane was performed. The obtained results were compared with the results carried out in 96-well plates. Based on these results, it was noted that the enzymatic reaction time in the microsystem is shorter than in 96-well plate. Moreover, the advantage of using microsystem is also the small amount of reagents.
Źródło:
Challenges of Modern Technology; 2012, 3, 4; 3-8
2082-2863
2353-4419
Pojawia się w:
Challenges of Modern Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of the Proposed Training Program on some Physiological and Body Composition Variables of under 20 Years Old Male Football Players
Autorzy:
Khanafdl, Omar Talha
Powiązania:
https://bibliotekanauki.pl/articles/1627265.pdf
Data publikacji:
2021-09-10
Wydawca:
Uniwersytet Szczeciński. Wydawnictwo Naukowe Uniwersytetu Szczecińskiego
Tematy:
aerobic training
anaerobic training
blood lactate
body composition
football player
Opis:
The purpose of the study was to find out the effect of the proposed training program on some physiological and body composition variables of 24 volunteered youth male football players who under 20 years old (U20, age: 16–19 years, mean age: 17.3 ±1.0 years, playing for last 4–6 years). Those 24 players were divided into 2 groups, 12 players (experimental group), 12 players (control group). The training program consisted of aerobic training, anaerobic training, recreational game and practice football game. Data was collected at zero level (pre-test), in the mid of the program (mid-test) and at the end of program (post-test). For analysing data, repeated measures were used. In the experimental group a significant increase (P < 0.05) in number of shuttles, skeletal muscle, and resting metabolism and a significant decrease (P < 0.05) in body fat have been noted in (mid-test) and (post-test) when compared to (pre-test). However, a decrease was noted in body mass index, blood lactate, maximal heart rate, and visceral fat, but not to a significantly different level. In the control group negative significant increase (P < 0.05) in body fat and negative significant decrease (P < 0.05) in skeletal muscle and resting metabolism have been noted, no significant difference was observed in number of shuttle, body mass index, blood lactate, maximum heart rate and visceral fat. This study would provide useful information for training and exercise physiology and it may have a beneficial impact on health.
Źródło:
Central European Journal of Sport Sciences and Medicine; 2021, 34, 2; 97-104
2300-9705
2353-2807
Pojawia się w:
Central European Journal of Sport Sciences and Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cold Water Immersion as a Method Supporting Post-Exercise Recovery
Autorzy:
Kowalski, Mateusz
Lubkowska, Anna
Powiązania:
https://bibliotekanauki.pl/articles/2138481.pdf
Data publikacji:
2022
Wydawca:
Uniwersytet Szczeciński. Wydawnictwo Naukowe Uniwersytetu Szczecińskiego
Tematy:
exercise recovery
cold water immersion
lactate concentration
Opis:
The aim of the study was to assess the effect of cold water immersion on changes in blood lactate concentration during post-exercise recovery in swimmers subjected to2-minute exercise test (front crawl swimming movements) using a VASA Swim Ergometer, with the maximum arm speed movements, as during the freestyle technique. The study covered 11 professional swimmers of the MKP Szczecin club, tested twice with a two-week interval. Each participant performed an exercise test twice, once with a passive recovery period, and the second time with cold water immersion after exercise, as a method potentially supporting the post-exercise recovery process. Each time before the test, immediately after and at 3, 6 and 9 minutes after exercise, the concentration of lactate in the capillary blood was measured. Statistical analysis of the obtained results showed that cold water immersion applied immediately after exercise resulted in a faster reduction of lactate concentration. The conducted research confirms that cold water immersion used in post-exercise recovery may be an effective method of restoring optimal physical fitness as part of the training process.
Źródło:
Central European Journal of Sport Sciences and Medicine; 2022, 38; 61-70
2300-9705
2353-2807
Pojawia się w:
Central European Journal of Sport Sciences and Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Newer Perspectives in Lactate Threshold Estimation for Endurance Sports – A Mini-Review
Autorzy:
Krishnan, Anup
Guru, Chandra Sekara
Sivaraman, Arumugam
Alwar, Thiagarajan
Sharma, Deep
Angrish, Piyush
Powiązania:
https://bibliotekanauki.pl/articles/1832381.pdf
Data publikacji:
2021
Wydawca:
Uniwersytet Szczeciński. Wydawnictwo Naukowe Uniwersytetu Szczecińskiego
Tematy:
anaerobic threshold
physical endurance
athletic performance
exercise testing
lactate threshold
Opis:
Lactate threshold (LT) estimation in endurance sports continues to be a widely controversial field amongst sports scientists and students despite beyond 50 years of research. With the advent of technology and superior sensors, LT research has ventured into newer fields involving wearables and artificial intelligence. Still, there is a felt need to understand the focused areas of LT research and to guide the students, sports scientists and coaches. The main aim of this mini-review is to identify research categories in a descriptive manner and to synthesize broad themes for future research from latest literature. A comprehensive electronic search in three databases was performed including only original free full text research articles conducted in athletes and heathy subjects, published in English between 2016 and 2020 following PRISMA guidelines. Out of screened 466 articles, 14 articles were finally shortlisted as per inclusion criteria and the findings were summarized. Five research categories were identified and reviewed. To conclude, there is a need for consensus in Graded Exercise test protocols used, LT concepts validity for specific sports and the application of valid, reliable noninvasive LT estimation methods in endurance sports. Synthesized broad themes would help guide sports scientists, students and researchers for future research.
Źródło:
Central European Journal of Sport Sciences and Medicine; 2021, 35, 3; 99-116
2300-9705
2353-2807
Pojawia się w:
Central European Journal of Sport Sciences and Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cardiovascular, metabolic and plasma catecholamine responses to passive and active exercises
Autorzy:
Krzeminski, K.
Kruk, B.
Nazar, K.
Ziemba, A.W.
Cybulski, G.
Niewiadomski, W.
Powiązania:
https://bibliotekanauki.pl/articles/70316.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Fizjologiczne
Tematy:
cardiovascular response
plasma adrenaline
heart rate
noradrenaline
stroke volume
plasma catecholamine
systolic time interval
passive exercise
metabolic response
blood pressure
active exercise
blood lactate
Źródło:
Journal of Physiology and Pharmacology; 2000, 51, 2
0867-5910
Pojawia się w:
Journal of Physiology and Pharmacology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Physical activity of elite football players using different regimes of energy metabolism
Aktywność fizyczna elitarnych piłkarzy nożnych w różnych reżimach metabolizmu energetycznego
Autorzy:
Lyzohub, V.
Kozhemiako, T.
Khomenko, S.
Pustovalov, V.
Shpaniuk, V.
Powiązania:
https://bibliotekanauki.pl/articles/2048062.pdf
Data publikacji:
2021
Wydawca:
Akademia Bialska Nauk Stosowanych im. Jana Pawła II w Białej Podlaskiej
Tematy:
telemetry
oxygen consumption
energy metabolism
lactate
Opis:
Background. The physical activity of 29 football players using either aerobic or anaerobic energy regimes was tested during official games and in the laboratory. Material and methods. Under conditions of gradually increasing power on a treadmill, maximal running speed (V, km/h), oxygen consumption (VO2, mL/min/kg), heart rate (HR, beats/min), and anaerobic metabolism threshold (AT mL/min/kg) were determined, and blood lactate (BLact, mmol/L) was measured. Results. The correlation between the total length of distance run during the game and treadmill running speed for the outcome measures were as follows: anaerobic metabolism threshold, r=0.54 (p=0.032), BLact, r=0.37 (p=0.034), VO2max, r=0.41 (p=0.027), and HR, r=0.36 (p=0.047). We found aerobic mechanisms were dominant in ensuring the game activity of football players. Specifically, aerobic mechanisms contribute roughly 32.4-43.8% to playing activity, while aerobic-anaerobic energy supply mechanisms provide for only 17%. Lactic and alactic mechanisms provide for 5.6% and 1.6%, respectively. Conclusions. The results provide valuable information on the relationship between football play activity and aerobic and anaerobic energy supply mechanisms. Enhancing the functionality of the anaerobic lactic-alactic energy system can lead to growth and improvements in physical performance in football players.
Źródło:
Health Problems of Civilization; 2021, 15, 3; 202-210
2353-6942
2354-0265
Pojawia się w:
Health Problems of Civilization
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Rumination time as an indicator of stress in the first thirty days after calving
Autorzy:
Malašauskienė, D.
Televičius, M.
Juozaitienė, V.
Antanaitis, R.
Powiązania:
https://bibliotekanauki.pl/articles/2087556.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
rumination time
cortisol
lactate
cow
Źródło:
Polish Journal of Veterinary Sciences; 2019, 2; 363-368
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Koncepcja procesu produkcji mleczanu etylu
Process design outline in the production of ethyl lactate
Autorzy:
Mitkowski, P. T.
Broniarz-Press, L.
Powiązania:
https://bibliotekanauki.pl/articles/2071174.pdf
Data publikacji:
2010
Wydawca:
Stowarzyszenie Inżynierów i Techników Mechaników Polskich
Tematy:
estryfikacja
mleczan etylu
dobór rozpuszczalnika
siła napędowa procesu
perwaporacja
ekstrakcja
esterification
ethyl lactate
solvent selection
driving force
pervaporation
extraction
Opis:
W pracy przedstawiono analizę produkcji mleczanu etylu z kwasu mlekowego i etanolu ukierunkowaną na podniesienie wydajności reakcji estryfikacji poprzez odwodnienie mieszaniny składników reagujących. Przeprowadzona analiza ujawniła możliwość wykorzystania w tym celu perwaporacji z membraną GFT-1005 i ekstrakcji przy użyciu n-dekanu, które posiadają zbliżoną wartość siły napędowej. Zidentyfikowano dwie możliwe konfiguracje procesu: (1) reakcji z perwaporacją i (2) reakcji z ekstrakcją.
Analysis of ethyl lactate production from lactic acid and ethanol is presented. Reaction mixture should be dehydrated in order to increase the efficiency of esterification reaction. It was pointed out that pervaporation with GFT-1005 membrane and extraction with n-decane as solvent could be used. Both of them had similar values of driving force. Two process configurations were identified: (1) reaction with pervaporation and (2) reaction with extraction.
Źródło:
Inżynieria i Aparatura Chemiczna; 2010, 4; 52-53
0368-0827
Pojawia się w:
Inżynieria i Aparatura Chemiczna
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Lactate / albumin ratio as prognostic tool for risk stratification in septic patients admitted to ICU
Autorzy:
Mourya, Vasant
Gupta, Rakesh
Yadav, Amlendu
Yadav, Rupesh
Powiązania:
https://bibliotekanauki.pl/articles/29432047.pdf
Data publikacji:
2023-12
Wydawca:
Towarzystwo Pomocy Doraźnej
Tematy:
Albumin
lactate
mortality
sepsis
Opis:
INTRODUCTION: Sepsis stands as the primary cause behind intensive care unit (ICU) admissions. The most critical parameters in sepsis management have been shown to be early recognition. Management delays have been associated with increased mortality and morbidity The aim of this study is to study the lactate/albumin (L/A) ratio as prognostic tool for risk stratification in septic patients admitted to ICU. MATERIALS AND METHODS: This prospective observational study was conducted with100 patients. Admitted in ICU with sepsis and septic shock were studied. Serum lactate/albumin ratio was calculated at the time of admission. Apache 2 and SOFA score was calculated at admission. All patients received initial treatment according standard protocol. All patients were followed up till discharge. An adverse outcome in terms of in hospital mortality, length of ICU stays and inotropic support was used in this study. RESULTS: Lactate/albumin ratio >1.5(AUC 0.89) correctly predicted in-hospital mortality among 27% patients with sensitivity and specificity of 90% and 78.6% respectively (p value =0.001). Lactate/albumin ratio <1.50 (AUC 0.73) correctly predicted length of ICU stays <72 hours among 17% patients with sensitivity and specificity of 85% and 58.8% respectively (p value =0.001). Lactate/albumin ratio >1.50 (AUC 0.91) correctly predicted requiring inotropic support among 36% patients with sensitivity and specificity of 83.7% and 89.5% respectively (p value =0.001). CONCLUSIONS: We concluded that lactate/albumin ratio was a stronger parameter than lactate, albumin, APACHE score and SOFA alone in predicting mortality, length of ICU stay and requiring noradrenaline inotropic support among sepsis patients in the ICU.
Źródło:
Critical Care Innovations; 2023, 6, 4; 11-22
2545-2533
Pojawia się w:
Critical Care Innovations
Dostawca treści:
Biblioteka Nauki
Artykuł
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