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Tytuł:
Non-destructive viscometric studies of enzymic milk coagulation III. The effect of pH, temperature and Ca-ions concentration on the secondary phase of milk coagulation
Nie niszczące badania wiskozymetryczne enzymatycznego procesu krzepnięcia mleka III. Wpływ stężenia jonów wapniowych, pH i temperatury na wtórną fazę enzymatycznego krzepnięcia mleka
Autorzy:
Bachman, S.
Klimczak, B.
Gasyna, Z.
Powiązania:
https://bibliotekanauki.pl/articles/1398347.pdf
Data publikacji:
1980
Wydawca:
Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
Tematy:
milk coagulation
immobilized rennin
Ca-ions
Opis:
It was observed that within the pH range between 5.57 and 6.37 the time of development of the secondary phase of the rennet milk coagulation is independent from temperatures between 25° and 35°C. Increased concentration of Ca-ions between 0.66 and 1.1 mg CaCl₂ /ml milk accelerates the formation of the secondary phase.
Kontynuując wcześniej prowadzone prace na temat enzymatycznego procesu krzepnięcia mleka [l, 2] w tej części przedstawiono badania wpływu pH oraz stężenia jonów wapniowych we współdziałaniu z temperaturą na wtórną fazę enzymatycznego krzepnięcia mleka. Do badań zastosowano unieruchomioną renninę. Stwierdzono, że dla wartości pH od 5,57 do 6,37 czas wystąpienia fazy wtórnej jest niezależny od temperatury w zakresie 25-35°C. W miarę zwiększenia wartości pH następował spadek szybkości zmiany lepkości mleka w okresie wtórnej fazy. Wyznaczono zależność stałej szybkości zmian lepkości od stężenia jonów wodorowych: k = k'(H+)0,5. Określono zależność odwrotności czasu wystąpienia fazy wtórnej od stężenia jonów wapniowych w granicach 0,66-1,1 mg CaCl₂ /ml mleka dla temperatur w zakresie 25-35°C. Stwierdzono przyspieszenie wystąpienia fazy wtórnej w miarę wzrostu stężenia jonów wapniowych w mleku.
Źródło:
Acta Alimentaria Polonica; 1980, 06, 3; 135-143
0137-1495
Pojawia się w:
Acta Alimentaria Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Properties of beta-galactosidase from cells of Streptococcus lactis 192 and Lactobacillus lactis F-16 strains
Właściwości beta-galaktozydazy z komórek szczepów Streptococcus lactis 192 i Lactobacillus lactis F-16
Autorzy:
Rymaszewski, J.
Cichosz, G.
Kujawski, M.
Powiązania:
https://bibliotekanauki.pl/articles/1398371.pdf
Data publikacji:
1985
Wydawca:
Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
Tematy:
desintegration of cell thermal stability
lactose
influence of ions
Streptococcus lactts
Lactobacmus lactts
Opis:
The β-galactosidase synthetized by cells of Str. lactis 192 and Lbc. lactis F-16 in characterized on the basis of pH and temperature optimum for the enzyme's activity, thermal stability and the effect of selected ions. There were significant differences in β-galactosidase activity depending on the strain and on the kind of extract. The Str. lactis 192 enzyme demonstrated higher activity in lactose fermentation and lower thenmal stability than the β-galactosidase of Lbc. lactis F-16. The effect of ions on enzyme activity was diversified and depended on the time of activity, the kind of extract and the bacteria strain.
Podjęto próbę charakterystyki β-galaktozydazy syntetyzowanej przez komórki Str. lactis 192 i Lbc. lactis F-16 na podstawie wyznaczenia optimum pH i temperatury działania enzymv, stabilności termicznej i wpływu wybranych jonów. Stwierdzono istotne różnice w aktywności β-galaktozydazy pomiędzy badanymi szczepami, a także rodzajami ekstraktów. Optimum działania β-galaktozydazy zawartej w odpowiednich ekstraktach przypadało w warunkach: - ekstrakt wewnątrzkomórkowy Str. lactis 192: temp. 308 K (35°C) i 318-323 K (45-50°C), pH 7.0, - ekstrakt ściany komórkowej Str. lactis 192: temp. 308 K (35°C) i pH 5,0, - ekstrakt wewnątrzkomórkowy Lbc. lactis F-16: temp. 323 K (50°C) i pH 6,0, - ekstrakt ściany komórkowej Lbc. lactis F-16: temp. 313 K (40°C) i pH 7,0. Enzym Str. lactis 192 wykazał wyższą aktywność fermentacji laktozy oraz niższą stabilność termiczną niż β-galaktozydaza Lbc. lactis F-16. Wpływ jonów na aktywność enzymu był zróżnicowany i zależny od czasu działania, rodzaju ekstraktu i szczepu bakterii. Oceniono również wpływ przechowywania komórek bakterii Str. lactis 192 w stanie zamrożonym i liofilizowanym na aktywność β-galaktozydazy. Zaobserwowano większy spadek aktywności β-galaktozydazy w przypadku preparatów liofilizowanych. Fakt ten należy tłumaczyć tym, że liofilizacji poddano komórki uprzednio zdezintegrowane, a ponadto nie stosowano żadnych substancji ochronnych podczas zamrażania i suszenia sublimacyjnego.
Źródło:
Acta Alimentaria Polonica; 1985, 11, 4; 449-460
0137-1495
Pojawia się w:
Acta Alimentaria Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A singular radially symmetric problem in electrolytes theory
Autorzy:
Nadzieja, Tadeusz
Raczyński, Andrzej
Powiązania:
https://bibliotekanauki.pl/articles/1339085.pdf
Data publikacji:
1998
Wydawca:
Polska Akademia Nauk. Instytut Matematyczny PAN
Tematy:
radial solutions
electrodiffusion of ions
nonlinear parabolic equation
Opis:
Existence of radially symmetric solutions (both stationary and time dependent) for a parabolic-elliptic system describing the evolution of the spatial density of ions in an electrolyte is studied.
Źródło:
Applicationes Mathematicae; 1998-1999, 25, 1; 101-112
1233-7234
Pojawia się w:
Applicationes Mathematicae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
On a nonlocal elliptic problem
Autorzy:
Raczyński, Andrzej
Powiązania:
https://bibliotekanauki.pl/articles/1338872.pdf
Data publikacji:
1999
Wydawca:
Polska Akademia Nauk. Instytut Matematyczny PAN
Tematy:
electrodiffusion of ions
nonlinear elliptic problem
theory of semiconductors
Opis:
We study stationary solutions of the system $u_t = ∇ ((m-1)/m ∇u^m + u∇φ)$, m => 1, Δφ = ±u, defined in a bounded domain Ω of $ℝ^n$. The physical interpretation of the above system comes from the porous medium theory and semiconductor physics.
Źródło:
Applicationes Mathematicae; 1999, 26, 1; 107-119
1233-7234
Pojawia się w:
Applicationes Mathematicae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of divalent metal ions on annexin-mediated aggregation of asolectin liposomes.
Autorzy:
Mel'gunov, Vladimir
Akimova, Elena
Krasavchenko, Kirill
Powiązania:
https://bibliotekanauki.pl/articles/1044309.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
aggregation
liposome
annexin
divalent metal ions
Opis:
Annexins belong to a family of Ca2+- and phospholipid-binding proteins that can mediate the aggregation of granules and vesicles in the presence of Ca2+. We have studied the effects of different divalent metal ions on annexin-mediated aggregation of liposomes using annexins isolated from rabbit liver and large unilamellar vesicles prepared from soybean asolectin II-S. In the course of these studies, we have found that annexin-mediated aggregation of liposomes can be driven by various earth and transition metal ions other than Ca2+. The ability of metal ions to induce annexin-mediated aggregation decreases in the order: Cd2+>Ba2+, Sr2+>Ca2+>>Mn2+>Ni2+>>Co2+. Annexin-mediated aggregation of vesicles is more selective to metal ions than the binding of annexins to membranes. We speculate that not every type of divalent metal ion can induce conformational change sufficient to promote the interaction of annexins either with two opposing membranes or with opposing protein molecules. Relative concentration ratios of metal ions in the intimate environment may be crucial for the functioning of annexins within specialized tissues and after treatment with toxic metal ions.
Źródło:
Acta Biochimica Polonica; 2000, 47, 3; 675-683
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Generation of ·OH initiated by interaction of Fe2+ and Cu+ with dioxygen; comparison with the Fenton chemistry.
Autorzy:
Urbański, Norbert
Beręsewicz, Andrzej
Powiązania:
https://bibliotekanauki.pl/articles/1044216.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
superoxide dismutase
autoxidation
catalase
free radical
Fenton reaction
hydroxyl radical
iron ions
copper ions
desferrioxamine
Opis:
Iron and copper toxicity has been presumed to involve the formation of hydroxyl radical (·OH) from H2O2 in the Fenton reaction. The aim of this study was to verify that Fe2+-O2 and Cu+-O2 chemistry is capable of generating ·OH in the quasi physiological environment of Krebs-Henseleit buffer (KH), and to compare the ability of the Fe2+-O2 system and of the Fenton system (Fe2+ + H2O2) to produce ·OH. The addition of Fe2+ and Cu+ (0-20 μM) to KH resulted in a concentration-dependent increase in ·OH formation, as measured by the salicylate method. While Fe3+ and Cu2+ (0-20 μM) did not result in ·OH formation, these ions mediated significant ·OH production in the presence of a number of reducing agents. The ·OH yield from the reaction mediated by Fe2+ was increased by exogenous Fe3+ and Cu2+ and was prevented by the deoxygenation of the buffer and reduced by superoxide dismutase, catalase, and desferrioxamine. Addition of 1 μM, 5 μM or 10 μM Fe2+ to a range of H2O2 concentrations (the Fenton system) resulted in a H2O2-concentration-dependent rise in ·OH formation. For each Fe2+ concentration tested, the ·OH yield doubled when the ratio [H2O2]:[Fe2+] was raised from zero to one. In conclusion: (i) Fe2+-O2 and Cu+-O2 chemistry is capable of promoting ·OH generation in the environment of oxygenated KH, in the absence of pre-existing superoxide and/or H2O2, and possibly through a mechanism initiated by the metal autoxidation; (ii) The process is enhanced by contaminating Fe3+ and Cu2+; (iii) In the presence of reducing agents also Fe3+ and Cu2+ promote the ·OH formation; (iv) Depending on the actual [H2O2]:[Fe2+] ratio, the efficiency of the Fe2+-O2 chemistry to generate ·OH is greater than or, at best, equal to that of the Fe2+-driven Fenton reaction.
Źródło:
Acta Biochimica Polonica; 2000, 47, 4; 951-962
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of Mg2+ on kinetics of oxidation of pyrimidines in duplex DNA by potassium permanganate.
Autorzy:
Łoziński, Tomasz
Wierzchowski, Kazimierz
Powiązania:
https://bibliotekanauki.pl/articles/1044147.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
oxidation of pyrimidines
thymine glycol
magnesium ions
rate constant of oxidation
pDS3 plasmid DNA
quantitative permanganate footprinting
Opis:
Potassium permanganate oxidation of pyrimidine bases is often used to probe single-stranded regions in functional DNA-protein complexes. However, so far reactivity of these bases in double-stranded DNA has not been studied quantitatively. We have investigated the kinetics of oxidation of pyrimidines in supercoiled pDS3 plasmid dsDNA by quantitative KMnO4 footprinting, in connection with parallel studies on the effect of Mg2+ on kinetics of oxidation of individual thymines in the single-stranded region of the open transcription complex of Escherichia coli RNA polymerase at a cognate Pa promoter contained in this plasmid. Rate constants of oxidation for pyrimidines, kj, in selected regions of pDS3 DNA, including Pa promoter, were determined under single-hit reaction conditions in the absence and presence of 10 mM MgCl2. Their values appeared to be sequence-dependent and were: (i) the largest for Ts in 5'TA3' and 5'TC3' steps, while 2-4 times smaller for 5'-adjacent ones in TT(A,G,C) and TTT(A) runs, (ii) for Cs in 5'TC3' steps 2-4 fold smaller than for adjacent Ts, and (iii) in the presence of Mg2+ generally larger by a sequence-dependent factor: in 5'TC3' steps of about 2 and 4 for Ts and Cs, respectively, in 5'TA3' steps of TTA and TTTA sequences for 3'-terminal Ts of about 3, while for their 5'-neighbors of a distinctly smaller value of about 2. Comparison of kj data for corresponding Ts located between +1 and -10 regions of Pa promoter in dsDNA and in ssDNA form in the open transcription complex, reported elsewhere, demonstrates that reactivity of pyrimidines in dsDNA is by 2-3 orders of magnitude smaller. The effect of Mg2+ in dsDNA is interpreted in terms of electrostatic barrier to diffusion of MnO4- on DNA surface, which is lowered by diffusive binding of these ions to backbone phosphates, involving also sequence-specific contacts with bases in the minor and major grooves of B-DNA.
Źródło:
Acta Biochimica Polonica; 2001, 48, 2; 511-523
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of diaphragms on measurements of ions emitted from dense magnetized plasmas
Autorzy:
Skladnik-Sadowska, E.
Sadowski, M.
Powiązania:
https://bibliotekanauki.pl/articles/146562.pdf
Data publikacji:
2001
Wydawca:
Instytut Chemii i Techniki Jądrowej
Tematy:
diagnostic of ions
energy distribution
plasma discharges
separate ions
Opis:
The paper concerns the diagnostics of ions escaping from dense magnetized plasma (DMP) discharges. Particular attention is paid to the basic problem of the separation of such ions from the investigated plasma, under the condition that this process should not disturb the velocity (and energy) distribution functions of the measured ions.
Źródło:
Nukleonika; 2001, 46, suppl. 1; 57-60
0029-5922
1508-5791
Pojawia się w:
Nukleonika
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Mg2+ ions do not induce expansion of the melted DNA region in the open complex formed by Escherichia coli RNA polymerase at a cognate synthetic Pa promoter. A quantitative KMnO4 footprinting study
Autorzy:
Łoziński, Tomasz
Wierzchowski, Kazimierz
Powiązania:
https://bibliotekanauki.pl/articles/1044146.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
permanganate footprinting
open promoter complex
thymine oxidation
effect of magnesium ions
RNA polymerase
Opis:
Footprinting studies of prokaryotic open transcription complexes (RPO), based on oxidation of pyrimidine residues by KMnO4 and/or OsO4 at a single oxidant dose, have suggested that the extent of DNA melting in the transcription bubble region increases in the presence of Mg2+. In this work, quantitative KMnO4 footprinting in function of the oxidant dose of RPO, using Escherichia coli RNA polymerase (Eσ70 ) at a fully functional synthetic promoter Pa having -35 and -10 consensus hexamers, has been used to determine individual rate constants of oxidation of T residues in this region at 37°C in the absence of Mg2+ and in the presence of 10 mM MgCl2, and to evaluate therefrom the effect of Mg2+ on the extent of DNA melting. Population distributions of end-labeled DNA fragments corresponding to oxidized Ts were quantified and analyzed according to the single-hit kinetic model. Pseudo-first order reactivity rate constants, ki, thus obtained demonstrated that Mg2+ ions bound to RPO merely enhanced the reactivity of all 11 oxidizable thymines between the +3 and -11 promoter sites by a position-dependent factor: 3-4 for those located close to the transcription start point +1 in either DNA strand, and about 1.6 for those located more distantly therefrom. On the basis of these observations, we conclude that Mg2+ ions bound to RPO at Pa do not influence the length of the melted DNA region and propose that the higher reactivity of thymines results mainly from lower local repulsive electrostatic barriers to MnO4- diffusion around carboxylate binding sites in the catalytic center of RPO and promoter DNA phosphates.
Źródło:
Acta Biochimica Polonica; 2001, 48, 2; 495-510
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Application of macrocycle compounds for metal ions separation and removal - a review
Autorzy:
Walkowiak, W.
Ulewicz, M.
Kozłowski, C. A.
Powiązania:
https://bibliotekanauki.pl/articles/346971.pdf
Data publikacji:
2002
Wydawca:
Politechnika Bydgoska im. Jana i Jędrzeja Śniadeckich. Wydział Technologii i Inżynierii Chemicznej
Tematy:
macrocycles
crown ethers
lariat ethers
metal ions
separation
Opis:
A review is presented on macrocyclic compounds novel reagents for metal ions separation and removal in solvent extraction, transport through liquid membranes, and flotation processes. As macrocyclic compounds were used such chemicals as non-ionizable crown ethers, ionizable crown ethers (lariat ethers), calixarenes, and acyclic polyethers. Many literature data as well our own recent research data will be reported on metal ions removal and separation.
Źródło:
Ars Separatoria Acta; 2002, 1; 87-98
1731-6340
Pojawia się w:
Ars Separatoria Acta
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A Superconducting Cyclotron as a primary accelerator for exotic beam facilities
Autorzy:
Maggiore, M.
Rifuggiato, D.
Calabretta, L.
Powiązania:
https://bibliotekanauki.pl/articles/146203.pdf
Data publikacji:
2003
Wydawca:
Instytut Chemii i Techniki Jądrowej
Tematy:
cyclotron
ions
medical
superconducting
Opis:
A four sector compact superconducting cyclotron for light ion beams with a maximum energy of 250 MeV/amu has been studied. This cyclotron is mainly designed to accelerate H2 + ions to be extracted by stripping. Ions like C, O or Ne can also be accelerated and extracted by stripping. Extraction by stripping allows to overcome many problems, especially if a certain beam intensity is requested. The preliminary design model of the magnet circuit has been accomplished with the 3D electromagnetic code OPERA [4]. The design of the main coils and of the cryostat has been investigated, too. The features of beam dynamics of this cyclotron, including extraction trajectories, will be presented. Several fields of application have been envisaged for this cyclotron, namely nuclear physics, therapy treatment, interdisciplinary research and radioisotope production using low or medium intensity beams.
Źródło:
Nukleonika; 2003, 48,suppl.2; 165-167
0029-5922
1508-5791
Pojawia się w:
Nukleonika
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Electron capture negative ion mass spectra of some freon derivatives
Autorzy:
Asfandiarov, N.
Pshenichnyuk, S.
Fal’ko, V.
Wnorowska, J.
Wnorowski, K.
Szamrej-Foryś, I.
Powiązania:
https://bibliotekanauki.pl/articles/148517.pdf
Data publikacji:
2003
Wydawca:
Instytut Chemii i Techniki Jądrowej
Tematy:
negative ions
electron capture
freon derivatives
Opis:
The temperature dependence of dissociative attachment has been investigated in the temperature range 350-575 K for two freon derivatives 1,1,1-C2H3F2Cl and C2F5Cl using Electron Capture Negative Ion Mass Spectra (ECNI MS). The temperature dependence for 1,1,1-C2H3F2Cl is similar to that observed in the case of CF3Cl reported by Illenberger et al. In contrast, C2F5Cl exhibits quite a different spectrum and its temperature behavior. It has been suggested that sigma*C-Cl delocalization onto two empty orbital leads to Cl- ion formation in two different resonance states.
Źródło:
Nukleonika; 2003, 48, 2; 83-88
0029-5922
1508-5791
Pojawia się w:
Nukleonika
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Magnetic system of the heavy ion cyclotron for track membranes production
Autorzy:
Alenitsky, Y.
Zaplatin, N.
Onischenko, L.
Samsonov, E.
Chesnov, A.
Powiązania:
https://bibliotekanauki.pl/articles/148719.pdf
Data publikacji:
2003
Wydawca:
Instytut Chemii i Techniki Jądrowej
Tematy:
cyclotron
magnetic system
sector shim
heavy ions beam
Opis:
A heavy ion fixed energy cyclotron based on a conventional magnet with a pole diameter of 1.6 m is designed and constructed in JINR. Ions with A/Z = 5 are accelerated up to the energy E = 2.4 MeV/nucleon. The isochronous magnetic field is formed by four pairs of sector shims. The beam phase shift in formed field is less than š15°RF, and the first harmonic of magnetic field is less than 3 G for all radii of the beam acceleration. Power consumption of magnet is 25 kW. The ECR source with an intensity of 3.5 × 1012 ions/s is used as an ion source. The extracted beam (~1011 ions/s) is intended for the track membrane production.
Źródło:
Nukleonika; 2003, 48,suppl.2; 55-57
0029-5922
1508-5791
Pojawia się w:
Nukleonika
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Status of the Warsaw ECR ion source and injection line
Autorzy:
Sudlitz, K.
Kulczycka, E.
Filipiak, B.
Górecki, A.
Powiązania:
https://bibliotekanauki.pl/articles/148684.pdf
Data publikacji:
2003
Wydawca:
Instytut Chemii i Techniki Jądrowej
Tematy:
axial injection
Electron Cyclotron Resonance (ECR)
highly charged ions
implantation
ion source
ion transport
Opis:
A room temperature home built 10 GHz ECR ion source delivers beams of B, C, N, O, F, Ne, S, Ar to the Cyclotron U200-P. The same ion source has also been used for surface irradiation of the solids. To upgrade the ion source and increase the ion current in the cyclotron an oven for evaporation of solid materials has been constructed and a two gap buncher has been installed in the injection line. Some new observations on the influence of the extraction system on the ion beam current will be presented.
Źródło:
Nukleonika; 2003, 48,suppl.2; 105-108
0029-5922
1508-5791
Pojawia się w:
Nukleonika
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Signalling: basics and evolution.
Autorzy:
Williams, Robert
Powiązania:
https://bibliotekanauki.pl/articles/1043264.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
pumps
signalling
metal ions
receptors
donors
substrates
Opis:
Signalling concerns the transfer of information from one body, a source, to another, a receiver in order to stimulate activity. The problem arises with the word information. It is defined as what is transferred in a sequence of things, say between people, e.g. words or signs. The idea of signalling between people is then obvious but it is not clear in cell biology. Information transfer, signalling, is required for the organisation of all cellular activity but we must ask what is transferred and how is it transmitted and received? Sometimes it is assumed that all information, i.e. organisation in a cell, is represented in the DNA sequence. This is incorrect. We shall show that the environment is a second source of information concerning material and energy. The receiving party from both DNA and the environment is general metabolism. The metabolism then signals back and sends information to both DNA and uptake from the environment. Even then energy is needed with machinery to send out all signals. This paper examines the way signalling evolved from prokaryotes through to man. In this process the environmental information received increased to the extent that finally the brain is a phenotypic as much as a genotypic organ within a whole organism. By phenotypic we mean it is organised by and interactive with information from the environment.
Źródło:
Acta Biochimica Polonica; 2004, 51, 2; 281-298
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł

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