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    Wyświetlanie 1-6 z 6
    Tytuł:
    DNAzyme as an efficient tool to modulate invasiveness of human carcinoma cells
    Autorzy:
    Wiktorska, Magdalena
    Papiewska-Pająk, Izabela
    Okruszek, Andrzej
    Sacewicz-Hofman, Izabela
    Niewiarowska, Jolanta
    Powiązania:
    https://bibliotekanauki.pl/articles/1040367.pdf
    Data publikacji:
    2010
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    cancer cells
    DNAzyme
    integrin
    Opis:
    In this study we evaluated efficiency of DNAzymes to modulate motility of cancer cells, an important factor in the progression and metastasis of cancers. For this purpose we targeted β1 integrins that are predominant adhesive receptors in various carcinoma cell lines (CX1.1, HT29, LOVO, LS180, PC-3). To evaluate invasiveness of cancer cells, we used a transwell migration assay that allowed analyzing chemotactic migration of colon carcinoma cell lines across an ECM-coated membrane. Their adhesive properties were also characterized by the analysis of adhesion to fibronectin, laminin and collagen. In addition, the expression of major integrin subunits, selected intact β1 integrins, and other adhesive receptors (ICAM, E-selectin, uPAR) was analyzed by flow cytometry. Inhibition of β1 integrin expression by DNAzyme to β1 mRNA almost abolished the invasiveness of the CX1.1, HT29, LS180, LOVO and PC-3 cells in vitro. These data show that DNAzymes to β1 integrin subunit can be used to inhibit invasiveness of carcinoma cells.
    Źródło:
    Acta Biochimica Polonica; 2010, 57, 3; 269-275
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of α3β1 and αvβ3 integrin glycosylation on interaction of melanoma cells with vitronectin
    Autorzy:
    Janik, Marcelina
    Przybyło, Małgorzata
    Pocheć, Ewa
    Pokrywka, Małgorzata
    Lityńska, Anna
    Powiązania:
    https://bibliotekanauki.pl/articles/1040422.pdf
    Data publikacji:
    2010
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    adhesion
    integrin
    glycosylation
    migration
    vitronectin
    Opis:
    The metastatic transformation of melanocytes is associated with altered expression of adhesion molecules, including αvβ3 and α3β1 integrins. Integrin αvβ3 is a primary vitronectin (VN) receptor, while both integrin types take part in adhesion to VN when they are in complex with uPAR. Although their role in melanoma cell interaction with VN is of great interest, the influence of N-oligosaccharides attached to these glycoproteins is still unappreciated. The present study assesses the role of αvβ3 and α3β1 integrins and the influence of their glycosylation status on WM9 and WM239 metastatic melanoma cell interactions with VN. Cell adhesion to and migration on VN were selected as the studied cell behaviour parameters. Functionblocking antibodies and swainsonine (SW) treatment were used in these tests. Both cell lines interacted with VN in an integrin-mediated but cell-line-specific manner. In WM9 cells, migration was not completely inhibited by antibodies against α3β1 or αvβ3 integrins, suggesting the participation of other VN receptors. In both cell lines in coprecipitation test the formation of an integrins/uPAR complex was shown. In the presence of SW formation of the complex did not occur, suggesting the participation of glycosylation in this proccess. Additionally, the adhesion properties of WM9 cells were changed after SW treatment. Our results suggest that in these two metastatic cell lines integrin-linked N-oligosaccharides influence the VN adhesion receptor activity and function.
    Źródło:
    Acta Biochimica Polonica; 2010, 57, 1; 55-61
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Regulation of β1 integrin expression in endothelial cells by chimeric tRNAVal ribozyme
    Autorzy:
    Papiewska-Pająk, Izabela
    Antoszczyk, Sławomir
    Powiązania:
    https://bibliotekanauki.pl/articles/1041244.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    integrins
    cell migration
    β1 integrin subunit
    hammerhead ribozyme
    Opis:
    To downregulate expression of the β1 integrin subunit in endothelial cells, plasmid encoding the ribozyme cassette containing hammerhead ribozyme flanked at the 5' terminus by tRNAVal and at the 3' terminus by constitutive transport element sequences was constructed. When used to transfect immortalized human endothelial cell line EA.hy 926, it selectively blocked the synthesis of the β1 integrin subunit and thus inhibited migration and proliferation of the cells. Thus, this construct may be a valuable tool to control the proangiogenic phenotype of stimulated endothelial cells.
    Źródło:
    Acta Biochimica Polonica; 2006, 53, 2; 311-316
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The structure of the oligosaccharides of α3β1 integrin from human ureter epithelium (HCV29) cell line.
    Autorzy:
    Lityńska, Anna
    Pocheć, Ewa
    Hoja-Łukowicz, Dorota
    Kremser, Elżbieta
    Laidler, Piotr
    Amoresano, Angela
    Monti, Chiara
    Powiązania:
    https://bibliotekanauki.pl/articles/1043787.pdf
    Data publikacji:
    2002
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    glycosylation
    α3β1 integrin
    cell line
    MALDI MS
    Opis:
    There is a growing line of evidence that glycosylation of α and β subunits is important for the function of integrins. Integrin α3β1, from human ureter epithelium cell - line HCV29, was isolated by affinity chromatography on laminin GD6 peptide. Characterization of its carbohydrate moieties was carried out using sodium dodecyl sulfate/polyacrylamide gel electrophoresis followed by Western blotting on Immobilon P and on-blot deglycosylation with peptide N-glycosidase-F. Profiles of N-glycans for each subunit were obtained by matrix-assisted laser desorption/ionization mass spectrometry. Our findings demonstrated, in both subunits of integrin α3β1, the presence of complex type oligosaccharides with a wide heterogeneity. Bi- tri- and tetraantennary structures were the most common, while high-mannose type structures were minor. Also the presence of short poly-N-acetyllactosamine entities was shown. These results show that while the predominant oligosaccharides of both subunits are identical, some slight differences between them do exist.
    Źródło:
    Acta Biochimica Polonica; 2002, 49, 2; 491-500
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Integrin αv signaling influences phenotype and maturation of primary human osteoblasts on alumina surface
    Autorzy:
    Wróbel, E.
    Witkowska-Zimny, M.
    Mrówka, P.
    Głodkowska-Mrówka, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/284664.pdf
    Data publikacji:
    2014
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    integrin
    human bone-derived cells
    osteogenic differentiation
    cell adhesion
    biomaterial
    Opis:
    Due to the growing interest in stem cells application in tissue engineering the better understanding of primary human osteoblasts behavior in vitro, on biomaterial surface, is required. Among other molecules integrins may be taken into account as being involved in these phenomena. Integrins are a family of cell adhesion receptors, which may regulate many cellular functions e.g., adhesion, motility, phenotype and cell maturation. The aim of this study was to determine the effect of the biomaterial surfaces and αv integrin signaling pathway on the behavior, phenotype and maturation of human osteoblasts in vitro. Human bone derived cells (HBDCs) obtained from adult femoral bone fragments were cultured on both alumina disks and tissue culture polystyrene (TCPS) dishes. After 7, 14, and 21 days of culture, localization and mRNA expression level of αv integrin subunits and BGLAP (osteocalcin) on polystyrene were analyzed in addition, we treated the cell cultures with monoclonal antibodies against human αv integrin to block its ligand-binding activity, on both alumina and TCPS substrates. We found that the αv integrin was present in focal contacts and cell cytoplasm at subsequent stages of cell maturation and the level of αv integrin mRNA was the highest in mature osteoblasts. Blocking αv integrin transduction pathway caused changes in cell activity and morphology, decreased cells proliferation on TCPS and reduced expression of alkaline phosphatase (ALP) on both materials. The results suggest that αv integrin is involved as an important receptor facilitating osteogenic differentiation.
    Źródło:
    Engineering of Biomaterials; 2014, 17, 127; 33-39
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Analysis of integrins and vascular endothelial growth factor isoforms mRNA expression in the canine uterus during perimplantation period
    Autorzy:
    Bukowska, D.
    Kempisty, B.
    Jackowska, M.
    Wozna, M.
    Antosik, P.
    Piotrowska, H.
    Jaskowski, J.M.
    Powiązania:
    https://bibliotekanauki.pl/articles/31799.pdf
    Data publikacji:
    2011
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    integrin
    vascular endothelial growth factor
    isoform
    mRNA expression
    dog
    uterus
    pregnancy
    bitch
    Opis:
    Integrins are the major receptors within the extracellular matrix (ECM) that mediate several functions connected with cell life and metabolism, such as cell adhesion, migration, cytoskeletal organization, proliferation, survival, and differentiation. A vascular endothelial growth factor (VEGF) is one of the most important angiogenic factors. It has been suggested that the expression of this gene may play crucial physiological roles in reproductive organs. All investigated endometrial tissues were isolated on day 10-12 after mating. Control bitches, used in this study, were in metestrus, which was determined according to the vaginal cytology and progesterone level in blood. Early pregnancy was verified by flushing the uterine horns with PBS. Total RNA was isolated from the bitches endometrium by means of the Chomczyński and Sacchi method, treated by DNase I, and reverse-transcribed into cDNA. A quantitative analysis of integrins α2b, β2 and β3, VEGF 164, 182 and 188 cDNA was performed by RT-PCR. In results we have shown an increased expression of all investigated genes (integrins α2b, β2 and β3, VEGF 164, 182, and 188) in pregnant bitches uterus as compared to non-pregnant females (P<0.001). Our results indicated that the expression of genes encoding integrins and vascular endothelial growth factors is different in relation to the time of the embryo implantation and it is increased in the first period of this process. This may be associated with the induction of specific mechanisms responsible for receptivity of uterus following the embryo attachment. In addition, all of investigated genes are up-regulated in a pregnancy-specific manner and the increased expression of these genes may regulate the uterus function during the implantation of canine embryos.
    Źródło:
    Polish Journal of Veterinary Sciences; 2011, 14, 2
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-6 z 6

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