- Tytuł:
- Large size dna in vitro and in vivo delivery using chitosan transfection
- Autorzy:
-
Sankov, Vladislav
Shagdarova, Balzhima
Varlamov, Valerii
Esipov, Roman
Svirshchevskaya, Elena - Powiązania:
- https://bibliotekanauki.pl/articles/1034546.pdf
- Data publikacji:
- 2017
- Wydawca:
- Sieć Badawcza Łukasiewicz - Polskie Towarzystwo Chitynowe
- Tematy:
-
DNA delivery
chitosan derivatives
hydrophobic groups
рRFP pmKate2 reporter plasmid - Opis:
- In modern medicine, many diseases can be treated using gene therapy, which requires a DNA delivery system to prevent DNA from degradation and to transport it to the cells. Liposomal reagents are expensive for such a therapy and new inexpensive biodegradable DNA carriers are required. Chitosan (Ch) is a cationic polymer with promising potency for gene delivery. Some Ch derivatives have been shown to efficiently transfect mammalian cells in vitro. However, there are many inconsistencies in the literature concerning the effectiveness of Ch systems for in vivo gene transfer. The aim of this work was to develop a Ch-based vector for in vivo delivery of a large-size DNA fragment coding for the far-red fluorescent protein (RFP). Among several Ch derivatives, hexanoyl-Ch (HCh) with a molecular weight of 20 kDa effectively transfected cells in vitro. Intratumoural injection of polyplexes in colon and lung tumours resulted in local expression of RFP in tumours.
- Źródło:
-
Progress on Chemistry and Application of Chitin and its Derivatives; 2017, 22; 190-200
1896-5644 - Pojawia się w:
- Progress on Chemistry and Application of Chitin and its Derivatives
- Dostawca treści:
- Biblioteka Nauki
Artykuł