- Tytuł:
- Hydrolysis of lactose from cheese whey using a reactor with [beta]-galactosidase enzyme immobilised on a commercial uf membrane
- Autorzy:
-
Regenhardt, S.A.
Mammarella, E.J.
Rubiolo, A.C. - Powiązania:
- https://bibliotekanauki.pl/articles/184957.pdf
- Data publikacji:
- 2013
- Wydawca:
- Polska Akademia Nauk. Czytelnia Czasopism PAN
- Tematy:
-
membrane bioreactor
immobilised enzyme
beta-galactosidas
lactose hydrolysis
bioreaktor membranowy
enzym unieruchomiony
beta-galaktozydazy
hydroliza laktozy - Opis:
- In this study, [Beta]-galactosidase enzyme from Kluyveromyces fragilis was immobilised on a commercial polyethersulfone membrane surface, 10 kDa cut-off. An integrated process, concerning the simultaneous hydrolysis–ultrafiltration of whey lactose was studied and working conditions have been fixed at 55[degrees]C and pH 6.9, the same conditions that are used for the industrial process of protein concentration. For the immobilisation, best results were obtained using 5% (v/v) of glutaraldehyde solution and 0.03 M galactose; the total activity recovery coefficient (TARC) was 44.2%. The amount of immobilised enzyme was 12.49 mg with a total activity of 86.3 LAU at 37[degrees]C, using 5% (w/v) lactose solution in phosphate buffer (100 mM pH 6.9). The stability of the immobilised enzyme was approximately 585 fold higher in comparison with the stability of free enzyme. Multipoint covalent immobilisation improves the stability of the enzyme, thereby enhancing the decision to use the membrane as a filtering element and support for the enzyme immobilisation.
- Źródło:
-
Chemical and Process Engineering; 2013, 34, 3; 375-385
0208-6425
2300-1925 - Pojawia się w:
- Chemical and Process Engineering
- Dostawca treści:
- Biblioteka Nauki
Artykuł