Temat: human antigen - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Genetic aspect of venom allergy: association with HLA class I and class II antigens
Autorzy:: Karakis, G P
Sin, B.A.
Tutkak, H.
Kose, K.
Misirligil, Z.
Powiązania:: https://bibliotekanauki.pl/articles/49628.pdf
Data publikacji:: 2010
Wydawca:: Instytut Medycyny Wsi
Tematy:: leucocyte
human leucocyte antigen class I
human leucocyte antigen class II
genetics
venom
allergy
sting allergy
insect
bee
wasp
Źródło:: Annals of Agricultural and Environmental Medicine; 2010, 17, 1; 119-123
1232-1966
Pojawia się w:: Annals of Agricultural and Environmental Medicine
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Search for mouse gene related to GA733 human tumor antigen gene
Autorzy:: Zielewicz, J
Skrzypczak, M.
Wojcierowski, J.
Powiązania:: https://bibliotekanauki.pl/articles/2048195.pdf
Data publikacji:: 1995
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: human antigen
glycoprotein
human genetics
mice
gene sequence
adenocarcinoma
cancer therapy
electrophoresis
carcinoma cell
bacteriophage
antigen
gene
mouse
oncoantigen
hybridization
tumour etiology
tumour
DNA
Opis:: Human antigen GA733-1, defined as 40 kDa cell glycoprotein, is one of the antigens associated with gastrointestinal carcinomas. Its studies may contribute to the tumor etiology and therapy effects in animal model.
Źródło:: Journal of Applied Genetics; 1995, 36, 3; 273-277
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Role of crossmatch testing when Luminex-SAB is negative in renal transplantation
Autorzy:: Jayant, Kumar
Reccia, Isabella
Julie, Bridson M
Sharma, Ajay
Halawa, Ahmed
Powiązania:: https://bibliotekanauki.pl/articles/1392546.pdf
Data publikacji:: 2018
Wydawca:: Index Copernicus International
Tematy:: renal transplant
human leukocyte antigen
HLA-typing
anti-HLA antibody
Opis:: The human leukocyte antigen (HLA) system plays an important role in the acceptance of renal graft. Long and better graft survival has been reported in patients with HLA-identical siblings and a nonreactive cytotoxicity assay (CDC). New methods of HLA-typing and anti-HLA antibody detection techniques such as flow cytometry, solid-phase immunoassays, or antigen bead assays have further improved the outcomes of renal transplant recipients. In the present review, the explicit details of these methodologies are discussed in detail.
Źródło:: Polish Journal of Surgery; 2018, 90, 1; 41-46
0032-373X
2299-2847
Pojawia się w:: Polish Journal of Surgery
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: The estimation of different ELISA procedures for serodiagnosis of human trichinellosis
Autorzy:: Moskwa, B.
Bień, J.
Cabaj, W.
Korinkova, K.
Koudela, B.
Kacprzak, E.
Stefaniak, J.
Powiązania:: https://bibliotekanauki.pl/articles/2144348.pdf
Data publikacji:: 2006
Wydawca:: Polskie Towarzystwo Parazytologiczne
Tematy:: parasite
human disease
serodiagnosis
diagnosis
trichinellosis
ELISA test
zoonotic disease
ELISA procedure
excretory-secretory antigen
Trichinella spiralis
human outbreak
Opis:: Introduction. The most important confirmative diagnostic test for trichinellosis is the presence of the muscle larvae in a tissue biopsy but this direct method has a low sensitivity of light and moderate infections. The aim of presented study was to compare the usefulness of the results obtained by three ELISA procedures for Trichinella spp. diagnosis in human outbreaks. Materials and methods. All sera (cases and controls) were tested for anti-Trichinella antibodies (immunoglobulin G) using commercially available Novatec KIT and two other ELISA procedures based on excretory-secretory (ES) antigens on Trichinella spiralis muscle larvae. The main differences in ELISA procedures were: the protein concentration in antigen, dilution of human serum samples, conjugate and the time of conjugate incubation. Additional differences were noticed in ES antigen preparation procedures as well as in T. spiralis isolates used in these procedures. Serum samples were obtained from 22 symptomatical patients from Poznań region (West Poland), geographic area where human outbreak had occurred. Control serum samples were obtained from 20 patients from an open population from a non endemic trichinellosis area. Results. The results were analyzed in terms of both: statistical and epidemiological point of view. Linear regression analysis and correlations coefficient r between OD values of total 22 patients obtained in three ELISA procedures were positive and high statistically significant. Three ELISA procedures revealed different cut-off values and positivity rates for outbreak. However, the majority of positive samples were found as positive in three procedures, but some of them were positive in two or one procedure only. These individual variability in sera reactivity observed in three ELISA procedures could be very important from epidemiological point of view.
Źródło:: Wiadomości Parazytologiczne; 2006, 52, 3; 231-238
0043-5163
Pojawia się w:: Wiadomości Parazytologiczne
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: The estimation of different ELISA procedures for serodiagnosis of human trichinellosis
Autorzy:: Moskwa, B
Bien, J.
Cabaj, W.
Korinkova, K.
Koudela, B.
Kacprzak, E.
Stefaniak, J.
Powiązania:: https://bibliotekanauki.pl/articles/839966.pdf
Data publikacji:: 2006
Wydawca:: Polskie Towarzystwo Parazytologiczne
Tematy:: parasite
human disease
serodiagnosis
diagnosis
trichinellosis
ELISA test
zoonotic disease
ELISA procedure
excretory-secretory antigen
Trichinella spiralis
human outbreak
Opis:: Introduction. The most important confirmative diagnostic test for trichinellosis is the presence of the muscle larvae in a tissue biopsy but this direct method has a low sensitivity of light and moderate infections. The aim of presented study was to compare the usefulness of the results obtained by three ELISA procedures for Trichinella spp. diagnosis in human outbreaks. Materials and methods. All sera (cases and controls) were tested for anti-Trichinella antibodies (immunoglobulin G) using commercially available Novatec KIT and two other ELISA procedures based on excretory-secretory (ES) antigens on Trichinella spiralis muscle larvae. The main differences in ELISA procedures were: the protein concentration in antigen, dilution of human serum samples, conjugate and the time of conjugate incubation. Additional differences were noticed in ES antigen preparation procedures as well as in T. spiralis isolates used in these procedures. Serum samples were obtained from 22 symptomatical patients from Poznań region (West Poland), geographic area where human outbreak had occurred. Control serum samples were obtained from 20 patients from an open population from a non endemic trichinellosis area. Results. The results were analyzed in terms of both: statistical and epidemiological point of view. Linear regression analysis and correlations coefficient r between OD values of total 22 patients obtained in three ELISA procedures were positive and high statistically significant. Three ELISA procedures revealed different cut-off values and positivity rates for outbreak. However, the majority of positive samples were found as positive in three procedures, but some of them were positive in two or one procedure only. These individual variability in sera reactivity observed in three ELISA procedures could be very important from epidemiological point of view.
Źródło:: Annals of Parasitology; 2006, 52, 3
0043-5163
Pojawia się w:: Annals of Parasitology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 6.

Tytuł:: Bacterially expressed truncated F2 domain of Plasmodium falciparum EBA-140 antigen can bind to human erythrocytes
Autorzy:: Rydzak, Joanna
Kryńska, Katarzyna
Suchanowska, Anna
Kaczmarek, Radosław
Łukasiewicz, Jolanta
Czerwiński, Marcin
Jaśkiewicz, Ewa
Powiązania:: https://bibliotekanauki.pl/articles/1039687.pdf
Data publikacji:: 2012
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: truncated F2 domain purification
human erythrocyte binding
EBA-140 antigen
Plasmodium falciparum
recombinant F2 domain expression
Opis:: The recently identified erythrocyte binding antigen-140 (EBA-140) is a member of the Plasmodium falciparum DBL family of erythrocyte binding proteins, which are considered as prospective candidates for malaria vaccine development. The EBA-140 ligand is a paralogue of the well-characterized P. falciparum EBA-175 antigen. They share homology of domain structure, including Region II, which consists of two homologous F1 and F2 domains and is responsible for ligand-erythrocyte interaction during invasion. It was shown that the F2 domain of EBA-175 antigen seems to be more important for erythrocyte binding. In order to study activity and immunogenicity of EBA-140 antigen F2 domain, it is necessary to obtain recombinant protein of high purity and in a sufficient amount, which used to pose a challenge due to the high content of disulphide bridges. Here, we present a new method for expression and purification of Plasmodium falciparum EBA-140 antigen F2 domain in E. coli Rosetta-gami strain in fusion with the maltose binding protein (MBP). The truncated F2 domain formed by spontaneous proteolytic degradation of the fusion protein was purified by affinity chromatography on Ni-NTA resin followed by size exclusion chromatography. Molecular mass of this protein was confirmed by mass spectrometry. Its N-terminal amino acid sequencing revealed a proteolytic cleavage site within the F2 domain. The proper folding of the recombinant, truncated F2 domain of EBA-140 antigen was confirmed by circular dichroism analysis. The truncated F2 domain can specifically bind to human erythrocytes but its binding is not as efficient as that of full Region II. This confirms that both the F1 and F2 domains of EBA-140 antigen are required for effective erythrocyte binding.
Źródło:: Acta Biochimica Polonica; 2012, 59, 4; 685-691
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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