- Tytuł:
- Propagation of non-adherent HL-60 cells in batch cultures maintained in staticand wave-type agitated systems
- Autorzy:
-
Wierzchowski, Kamil
Grabowska, Iwona
Pilarek, Maciej - Powiązania:
- https://bibliotekanauki.pl/articles/952663.pdf
- Data publikacji:
- 2019
- Wydawca:
- Polska Akademia Nauk. Czytelnia Czasopism PAN
- Tematy:
-
disposable (single-use) bioreactor
wave-typeagitation
non-adherentcellculture
human HL-60cells
bioreaktor jednorazowego użytku
falowanie
nieprzylegająca hodowla komórkowa
ludzkie komórki HL-60 - Opis:
- Typically applied static (i.e. non-agitated) cultures do not provide sufficient conditions for efficient propagation of suspended non-adherent cells, in general. Feasibility of small-scale wave-type agitated single-use bioreactors for gentle agitation underlies applicability of such systems for scaling-up of fragile biomass of animal cells. The basic aim of the study was to compare the results of non-adherent HL-60 cell propagation performed referentially as the batch culture in typical static (i.e. non-agitated) disposable culture flasks (50 cm3 of culture medium) and in ReadyToProcess WAVETM25 bioreactor system (GE Healthcare) equipped with disposable culture bag (300 cm3 of culture medium) subjected to continuous wave-type agitation. The density and viability of HL-60 cells were significantly higher for the bioprocess subjected to wave-type agitation, than in the reference static culture. The values of the specific rate of glucose consumption per cell (rglc=cell) exhibited by HL-60 cells maintained in the system with continuous wave-type agitation was significantly lower (i.e. up to more than 42%) than the values noted for the static culture, for exactly the same time-points of two compared cultures. The results of the studies undoubtedly and comprehensively confirmed the applicability of the studied disposable bioreactor with wave-induced agitation as the right platform for proceeding the propagation of non- adherent HL-60 cells and for providing the culture conditions required by HL-60 cells for sustainable metabolism.
- Źródło:
-
Chemical and Process Engineering; 2019, 40, 2; 167-177
0208-6425
2300-1925 - Pojawia się w:
- Chemical and Process Engineering
- Dostawca treści:
- Biblioteka Nauki
Artykuł