- Tytuł:
- Molecular cloning and functional expression of human cytosolic acetyl-CoA hydrolase
- Autorzy:
-
Suematsu, Naoya
Isohashi, Fumihide - Powiązania:
- https://bibliotekanauki.pl/articles/1041214.pdf
- Data publikacji:
- 2006
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
cDNA sequence
Spodoptera frugiperda
PCR
functional expression
housekeeping-type promoter
acetyl-CoA hydrolase - Opis:
- A cDNA encoding human cytosolic acetyl-CoA hydrolase (CACH) was isolated from a human liver cDNA library, sequenced and functionally expressed in insect cells. The human CACH cDNA encodes a 555-amino-acid sequence that is 81.4%/78.7% identical to those of the mouse/rat homologue, suggesting a conserved role for this enzyme in the human and rodent livers. Bioinformatical study further reveals a high degree of similarity among the human and rodent CACHs as follows: First, the gene is composed of 15 exons ranging in size from 56 to 157 bp. Second, the protein consists of two thioesterase regions and a C-terminal steroidogenic acute regulatory protein-related lipid transfer (START) domain. Third, the promoter region is GC-rich and contains GC boxes, but lacks both TATA and CCAAT boxes, the typical criteria of housekeeping genes. A consensus peroxisome proliferator responsive element (PPRE) present in the rodent CACH promoter regions supports marked CACH induction in rat liver by peroxisome proliferator (PP).
- Źródło:
-
Acta Biochimica Polonica; 2006, 53, 3; 553-561
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł