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    Wyświetlanie 1-14 z 14
    Tytuł:
    The xCELLigence system for real-time and label-free analysis of neuronal and dermal cell response to Equine Herpesvirus type 1 infection
    Autorzy:
    Golke, A.
    Cymerys, J.
    Slonska, A.
    Dzieciatkowski, T.
    Chmielewska, A.
    Tucholska, A.
    Banbura, M.W.
    Powiązania:
    https://bibliotekanauki.pl/articles/30665.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    xCELLigence system
    real-time analysis
    label-free analysis
    neuronal cell response
    dermal cell response
    cell response
    equine herpesvirus 1
    animal infection
    herpesvirus
    neuron
    neuronal cell
    dermal cell
    Opis:
    Real-time cell electronic sensing (RT-CES) based on impedance measurements is an emerging technology for analyzing the status of cells in vitro. It allows label-free, real time monitoring of the biological status of cells. The present study was designed to assess dynamic data on the cell processes during equine herpesvirus type 1 (EHV-1) infection of ED (equine dermal) cells and primary murine neuronal cell culture. We have demonstrated that the xCELLigence system with dynamic monitoring can be used as a rapid diagnostic tool both to analyze cellular behavior and to investigate the effect of viral infection.
    Źródło:
    Polish Journal of Veterinary Sciences; 2012, 15, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Survival rate of Suid Herpesvirus (SuHV-1, Aujeszky`s disease virus, ADV) in composted sewage sludge
    Autorzy:
    Paluszak, Z.
    Lipowski, A.
    Ligocka, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/30422.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    survival rate
    Suid herpesvirus 1
    herpesvirus
    composted sewage sludge
    Aujeszky's disease
    Aujeszky's disease virus
    Opis:
    Sewage sludge constitutes a source of valuable biogenic raw materials, but it is a carrier of many pathogenic microorganisms and viruses. Subjected to an effective sanitization by means of the process of composting, it is suitable to use in agriculture as fertilizers. The aim of this study was to observe the survival rate of Suid Herpesvirus under the influence of the temperature alone (water bath) as well as in sewage sludge subjected to the process of composting (pile). The samples were taken at different time intervals, and the virus titres were determined. The viruses survived considerably longer under laboratory conditions: at 30°C as long as 21 days, at 40°C - 93 hours, and at 50°C - less than an hour. In the compost pile, in spite of the lack of the thermophylic phase, the total survival time of the viruses ranged from 34 to 44.5 hours, which indicates the vast importance of other physicochemical factors, apart from the temperature, contributing to virus inactivation.
    Źródło:
    Polish Journal of Veterinary Sciences; 2012, 15, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Seroprevalence of Toxoplasma gondii, varicella zoster virus and human parvovirus B19 among women in the Biała Podlaska District of Eastern Poland
    Autorzy:
    Tokarska-Rodak, M.
    Paszkiewicz, J.
    Laskowski, K.
    Plewik, D.
    Chwedczuk, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/2088204.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Opolski. Instytut Nauk o Zdrowiu
    Tematy:
    human parvovirus B19
    toxoplasma
    herpesvirus 3
    human
    Polska
    women
    Opis:
    Background: Infections in pregnant women or women planning pregnancy caused by the protozoan Toxoplasma gondii and the viruses varicella zoster virus (VZV) and human parvovirus B19 can be a danger to the fetus. Aim of the study: The aim of the study was to determine the serological status of women of childbearing age in relation to T. gondii, VZV and human parvovirus B19 in a region of Eastern Poland (Biała Podlaska District). Material and methods: The study group consisted of 174 women aged 19 to 35 (average 23, SD 3.68) from the Biała Podlaska District. Anti-T. gondii IgM/IgG antibodies, anti-VZV IgG and anti-human parvovirus B19 IgG were detected by ELISA. Results: Serological screening revealed that the most common antibodies were anti-VZV and anti-parvovirus B19 (in 96% and 60.9% of women, respectively). Anti-T. gondii antibodies were found in 28.6%. No correlation was found between the presence of anti-T. gondii, human parvovirus B19, and VZV antibodies and the age of the examined women, their place of residence, and their education. Conclusions: About 4%, 39% and 71.2% of women participating in this study were still susceptible to infection with VZV, human parvovirus B19, and T. gondii, respectively. It is therefore important to address health education primarily in women of childbearing age in order to help them undertake relevant measures for prevention of T. gondii, human parvovirus B19 and VZV infection.
    Źródło:
    Medical Science Pulse; 2019, 13, 4; 17-22
    2544-1558
    2544-1620
    Pojawia się w:
    Medical Science Pulse
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Seroprevalence of BHV-1 (bovine herpesvirus type 1) among non-vaccinated dairy cattle herds with respiratory disorders
    Autorzy:
    Rypula, K.
    Ploneczka-Janeczko, K.
    Kita, J.
    Kumala, A.
    Zmudzinski, J.F.
    Powiązania:
    https://bibliotekanauki.pl/articles/30913.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    seroprevalence
    bovine herpesvirus type 1
    non-vaccinated animal
    dairy cattle
    herd
    respiratory disorder
    ELISA test
    seroconversion
    Opis:
    The objective of this study was to estimate a herd-level seroprevalence of bovine herpesvirus type 1 (BHV-1) in herds with clinical symptoms of the respiratory tract. Eighty-three herds with suspected BHV-1 infection were selected and divided into two categories with respect to their size: small (n=27) and large herds (n=56). Samples were collected from calves, heifers and cows older than 24 months. Seroprevalence was determined using the gB ELISA test. The herd level seroprevalence was estimated as 53% (44/83) in the tested herds, 11.1% (3/27) in the small herds and 73.2% (41/56) in the large herds. Our study suggests that the current biosecurity measures still warrant improvement.
    Źródło:
    Polish Journal of Veterinary Sciences; 2012, 15, 3
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Serological evidence of lack of contact with caprine herpesvirus type 1 and bluetongue virus in goat population in Poland
    Autorzy:
    Czopowicz, M.
    Kaba, J.
    Szalus-Jordanow, O.
    Nowicki, M.
    Witkowski, L.
    Frymus, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/30762.pdf
    Data publikacji:
    2010
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    serological evidence
    caprine herpesvirus type 1
    bluetongue virus
    goat
    population
    Polska
    seroprevalence
    small ruminant
    animal infection
    Opis:
    Investigation into herd-level seroprevalence of caprine herpesvirus type 1 (CpHV-1) and bluetongue virus (BTV) was conducted in 2007 in Poland. It involved the entire population of goats covered by a milk recording program in 2007, which included 49 goat herds. The number of goats examined in each herd was determined statistically in order to detect the presence of at least one seropositive animal in a herd with a 95% probability and simple random method of sampling was applied. No antibodies to CpHV-1 or BTV were detected. Further calculations were carried out to determine the herd-level true seroprevalence, taking into account sensitivity and specificity of the test as well as several other factors. It can be concluded that till the middle of 2007 population of Polish goats covered by the milk recording program remained negative with respect to CpHV-1 and BTV.
    Źródło:
    Polish Journal of Veterinary Sciences; 2010, 13, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Reactive oxygen metabolitesin alpha-herpesvirus-seropositive Mediterranean buffaloes (Bubalus bubalis): a preliminary study
    Autorzy:
    Tafuri, S.
    Marullo, A.
    Ciani, F.
    Della Morte, R.
    Montagnaro, S.
    Fiorito, F.
    De Martino, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087691.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    Bubalus bubalis
    Bubaline herpesvirus 1 (BuHV-1)
    Bovine herpesvirus 1 (BoHV-1)
    reactive oxygen metabolites (ROM)
    oxidative stress index (Osi)
    Źródło:
    Polish Journal of Veterinary Sciences; 2018, 21, 3; 639-642
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    HybProbes-based real-time PCR assay for rapid detection of equine herpesvirus type 2 DNA
    Autorzy:
    Osinska, E.
    Golke, A.
    Slonska, A.
    Cymerys, J.
    Banbura, M.W.
    Dzieciatkowski, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/30252.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    rapid detection
    equine herpesvirus
    real-time polymerase chain reaction
    horse
    herpesvirus
    Gammaherpesvirinae
    Rhadinovirus
    veterinary virology
    Opis:
    Equid herpesvirus type 2 (EHV-2) together with equid herpesvirus type 5 are members of Gammaherpesvirinae subfamily, genus Rhadinovirus. EHV-2 is one of major agents causing diseases of horses common worldwide. A possible role of EHV-2 in reactivating latent equid herpesvirus type-1 has been suggested, because reactivation of latent EHV-1 was always accompanied by EHV-2 replication. Variety techniques, including cell culture, PCR and its modifications, have been used to diagnose EHV-2 infections. The aim of this study was to develop, optimize and determine specificity of real-time PCR (qPCR) for EHV-2 DNA detection using HybProbesR chemistry and to evaluate clinical samples with this method. The analytical sensitivity of assay was tested using serial dilutions of viral DNA in range between 70 and 7x105 copies/ml. The limit of detection (LOD) was calculated using probit analysis and was determined as 56 copies/ml. In further studies 20 different clinical samples were tested for the presence of EHV-2. Described in-house qPCR method detected viral DNA in 5 of 20 specimens used. The results of this work show that developed HybProbes-based real-time PCR assay is very reliable and valuable for detection and quantification of equid herpesvirus type 2 DNA in different clinical samples. The high level of sensitivity, accuracy and rapidity provided by the LightCycler 2.0 instrument are favorable for the use of this system in the detection of EHV-2 DNA in veterinary virology.
    Źródło:
    Polish Journal of Veterinary Sciences; 2012, 15, 3
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Feline herpesvirus-1: Ocular manifestations, diagnosis and treatment options
    Choroby oczu kotów wywołane zakażeniami herpesvirusem-1: Objawy kliniczne, diagnostyka i sposoby leczenia
    Autorzy:
    Gould, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/9976.pdf
    Data publikacji:
    2012
    Wydawca:
    Okulistyka Weterynaryjna
    Tematy:
    cat
    animal disease
    feline herpesvirus 1
    diagnosis
    treatment
    ocular manifestation
    keratitis
    conjunctivitis
    animal exposure
    ocular disease
    Opis:
    W swojej pracy autor przedstawia kluczowe aspekty związane z chorobami oczu występującymi u kotów, które  powoduje  herpesvirus  koci typu 1. Praca  w obszerny sposób omawia wiele chorób implikowanych tym typem wirusa. szczegółowo opisuje objawy kliniczne poszczególnych schorzeń, diagnostykę oraz doradza w sposób praktyczny dobieranie odpowiedniego leczenia. Mamy podany szereg leków wraz z dawkami i sposobami ich podania dla poszczególnych schematów leczenia. Praca jest bogato ilustrowana fotografiami obrazującymi konkretne przypadki kliniczne. Autor włożył wiele wysiłku, dzięki czemu powstała naprawdę wspaniała praca naukowa, która praktykom daje gotowy schemat rozpoznawania i leczenia tych trudnych z punktu klinicznego chorób.
    Practical relevance Feline herpesvirus-1 (FHV-1) is a major cause of feline morbidity. Following exposure to the virus, virtually all cats become persistently infected and many of these will develop recrudescent disease on one or more occasions during their life. Ocular manifestations include conjunctivitis, corneal ulceration and keratitis. In severe cases the condition can be blinding. Global importance FHV-1 has a worldwide distribution, with reported exposure rates in some cat populations of up to 97%. As such it is a significant cause of clinical disease in the global cat population. Patient Group Young and adolescent cats are most at risk of acute primary disease, and the vast majority of these will then become persistently infected. Around half of all persistently infected cats will shed virus at some stage in their life and these may develop recrudescent ocular disease. Clinical challenges Acute ocular herpetic disease can be severe and painful. Repeated bouts of recrudescent ocular disease can lead to progressive corneal pathology that can be ultimately blinding in affected cats. Treatment of FHV-1 ocular disease is challenging. Antiviral medications may be expensive and require good owner and patient compliance. Clinical responses in patients can be variable. Selecting the appropriate therapeutic approach requires good clinical judgement and depends on a number of factors such as severity and stage of clinical disease, patient and owner compliance and financial considerations. Evidence base Although a wide range of antiviral treatments are available, very few of these have been tested in controlled clinical trials. Instead, therapeutic decisions are often made based on results of in vitro studies, case-based reports and anecdote. Large, masked, controlled clinical trials are required in order to determine the efficacy of the antiviral drugs currently available to treat FHV-1.
    Źródło:
    Okulistyka Weterynaryjna. e-kwartalnik dla lekarzy i studentów weterynarii; 2012, 2
    2082-9256
    Pojawia się w:
    Okulistyka Weterynaryjna. e-kwartalnik dla lekarzy i studentów weterynarii
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Equine herpesvirus type 1 (EHV-1) replication in primary murine neurons culture
    Autorzy:
    Cymerys, J.
    Dzieciatkowski, T.
    Slonska, A.
    Bierla, J.
    Tucholska, A.
    Chmielewska, A.
    Golke, A.
    Banbura, M.W.
    Powiązania:
    https://bibliotekanauki.pl/articles/31974.pdf
    Data publikacji:
    2010
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    replication
    animal disease
    horse
    equine herpesvirus 1
    respiratory tract infection
    neurological disorder
    abortion
    neuron
    Opis:
    Equine herpesvirus-1 (EHV-1) infections cause significant economic losses for equine industries worldwide as a result of abortion, respiratory illness, and neurologic disease in all breeds of horses. The occurrence of abortions caused by EHV-1 has repeatedly been confirmed in Poland, but neurological manifestations of the infection have not been described yet. Also it is unknown how the infection of neurons with non-neuropathogenic strains is regulated. To further understand the virus- neuron interaction we studied two strains of EHV-1 in murine primary neuron cell cultures. Both strains were isolated from aborted fetuses: Rac-H, a reference strain isolated by Woyciechowska in 1959 (Woyciechowska 1960) and Jan-E isolated by Bańbura et al. (Bańbura et al. 2000). Upon infection of primary murine neuronal cell cultures with Jan-E or Rac-H strains, a cytopathic effect was observed, manifested by a changed morphology and disintegration of the cell monolayer. Positive results of immunofluorescence, nPCR and real-time PCR tests indicated high virus concentration in neurons, meaning that both EHV-1 strains were likely to replicate in mouse neurons in vitro without the need for adaptation. Moreover, we demonstrated that some neurons may survive (limited) virus replication during primary infection, and these neurons (eight weeks p.i.) harbour EHV-1 and were still able to transmit infection to other cells.
    Źródło:
    Polish Journal of Veterinary Sciences; 2010, 13, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Equine herpesvirus type 1 affects mitochondrial network morphology and reactive oxygen species generation in equine dermal cell line
    Autorzy:
    Bartak, M.
    Chodkowski, M.
    Słońska, A.
    Bańbura, M.W.
    Cymerys, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087318.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    equine herpesvirus type 1
    equine dermal cell line
    mitochondria
    reactive oxygen species
    Źródło:
    Polish Journal of Veterinary Sciences; 2020, 23, 2; 309-312
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of administering activated lymphocytes originated from the dam on the immune cell reaction in Holstein calves
    Autorzy:
    Ohtsuka, H.
    Yamaguchi, T.
    Maeda, Y.
    Tomioka, M.
    Tajima, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087343.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    bovine herpesvirus
    calves
    dam
    immune cells
    lymphokine activated killer
    Źródło:
    Polish Journal of Veterinary Sciences; 2020, 23, 1; 109-117
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Development of an indirect ELISA based on glycoprotein B gene for detecting of Feline herpesvirus type 1
    Autorzy:
    Tan, Y.
    Dong, G.
    Niu, J.
    Guo, Y.
    Yi, S.
    Sun, M.
    Wang, K.
    Hu, G.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087468.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    feline herpesvirus type 1
    gB protein
    indirect ELISA
    prokaryotic expression
    Źródło:
    Polish Journal of Veterinary Sciences; 2019, 3; 631-633
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    An in vitro assessment of antiviral activity for ethanol extract of Desmodium canadense against bovine herpesvirus type 1
    Autorzy:
    Lelešius, R.
    Girdauskaitė, P.
    Karpovaitė, A.
    Mickienė, R.
    Drevinskas, T.
    Tiso, N.
    Ragažinskienė, O.
    Kubilienė, L.
    Maruška, A.
    Šalomskas, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087312.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    bovine herpesvirus 1
    plant extracts
    Desmodium canadense
    Źródło:
    Polish Journal of Veterinary Sciences; 2020, 23, 2; 177-184
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A method for the infectivity discrimination of enveloped DNA viruses using palladium compounds pre-treatment followed by real-time PCR
    Autorzy:
    Krzyzankova, M.
    Krasna, M.
    Prodelalova, J.
    Vasickova, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/16647567.pdf
    Data publikacji:
    2023
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    African swine fever virus
    bovine herpesvirus-1
    palladium compounds
    platinum compounds
    molecular methods
    viability PCR
    Opis:
    Cultivation-based assays represent the gold standard for the assessment of virus infectivity; however, they are time-consuming and not suitable for every virus type. Pre-treatment with platinum (Pt) compounds followed by real-time PCR has been shown to discriminate between infectious and non-infectious RNA viruses. This study examined the effect of Pt and palladium (Pd) compounds on enveloped DNA viruses, paying attention to two significant pathogens of livestock – bovine herpesvirus-1 (BoHV-1) and African swine fever virus (ASFV). Native or heat-treated BoHV-1 suspension was incubated with the spectrum of Pt/Pd compounds. Bis(benzonitrile)palladium(II) dichloride (BB-PdCl 2) and dichloro(1,5-cyclooctadiene) palladium(II) (PdCl 2-COD) produced the highest differences found between native and heat- -treated viruses. Optimized pre-treatment conditions (1 mM of Pd compound, 15 min, 4°C) were applied on both virus genera and the heat inactivation profiles were assessed. A significant decrease in the detected quantity of BoHV-1 DNA and ASFV DNA after heat-treatment (60°C and 95°C) and consequent incubation with Pd compounds was observed. BB-PdCl 2 and PdCl 2-COD could help to distinguish between infectious and non-infectious enveloped DNA viruses such as BoHV-1 or ASFV.
    Źródło:
    Polish Journal of Veterinary Sciences; 2023, 26, 2; 211-221
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-14 z 14

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