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Tytuł:
A well-oxygenated cells environment may help to fight against protein glycation
Autorzy:
Mercier, Béatrice
Prost, Josiane
Prost, Michel
Powiązania:
https://bibliotekanauki.pl/articles/2185469.pdf
Data publikacji:
2011-03-01
Wydawca:
Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
Tematy:
Bol d’Air Jacquier®
peroxidizing terpenes
glycated hemoglobin
oxygen
Opis:
Objectives: Normoglycemic Wistar rats’ Glycated Hemoglobin Levels (GHL) showed a time-dependent difference between control groups and those exposed to regular inhalation of peroxidizing extracts of turpentine. These extracts were able to optimize the oxygen permeation at the cellular level during and subsequently to a breathing session. The more the rats breathed turpentine peroxidized vapor, the lower their GHL was. This study was designed to confirm, in ex-vivo blood samples, the impact of peroxidizing extract on the GHL. Materials and Methods: Red blood cells were separated from plasmas by centrifugation. Plasmas were treated by peroxidizing and non-peroxidizing turpentine vapor or untreated (control), then combined with washed red blood cells three hours before evaluation. Glycation of hemoglobin proteins was quantified according to the Habeed’s method. Results: The ex-vivo experiments showed that the peroxidizing terpenes reduced the GHL after a three-hour contact. So did oxidized terpenes. Controls and the volatile component of the expended essential oil showed the opposite results. Conclusion: Optimal oxygenation, especially when facilitated by the peroxidized volatile component of the essential oil of turpentine, can protect organisms (mammals in this study) from protein glycation. Optimizing oxygenation can also reduce the GHL of treated blood samples after three hours of incubation.
Źródło:
International Journal of Occupational Medicine and Environmental Health; 2011, 24, 1; 102-107
1232-1087
1896-494X
Pojawia się w:
International Journal of Occupational Medicine and Environmental Health
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Analysis of oxygen binding by hemoglobin on the basis of mean intrinsic thermodynamic quantities
Autorzy:
Bordbar, Abdol-Khalegh
Mousavi, Sayed
Dazhampanah, Hamid
Powiązania:
https://bibliotekanauki.pl/articles/1041216.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
cooperativity
mean intrinsic Gibbs free energy
linkage phenomena
hemoglobin
Opis:
The binding data for oxygenation of human hemoglobin, Hb, at various temperatures and in the absence and presence of 2,3-diphosphoglycerate, DPG, and inositol hexakis phosphate, IHP, were analyzed for extraction of mean intrinsic Gibbs free energy, ΔGo, enthalpy, ΔHo, and entropy, ΔSo, of binding at various partial oxygen pressures. This method of analysis considers all the protein species present such as dimer and tetramer forms which were not considered by Imai et al. (Imai K et al., 1970, Biochim Biophys Acta 200: 189 - 196) , in their analysis which was based on Adair equation. In this regard, the values of Hill equation parameters were estimated with high precision at all points of the binding curve and used for calculation of ΔGo, ΔHo and ΔSo were also calculated by analysis of ΔGo values at various temperatures using van't Hoff equation. The results represent the enthalpic nature of the cooperativity in Hb oxygenation and the compensation effect of intrinsic entropy. The interpretation of results also to be, into account the decrease of the binding affinity of sites for oxygen in the presence of DPG and IHP without any considerable changes in the site - site interaction (extent of cooperativity). In other words, the interactions between bound ligands, organic phosphates and oxygen, are more due to a decreasing binding affinity and not to the reduction of the cooperative interaction between sites. The results also document the more heterotropic effect of IHP compared to DPG.
Źródło:
Acta Biochimica Polonica; 2006, 53, 3; 563-568
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Are we helping or harming our insulin-treated diabetic patients during ambulatory treatment?
Autorzy:
Szczesniak, G.
Dziemidok, P.
Paprzycki, P.
Powiązania:
https://bibliotekanauki.pl/articles/50293.pdf
Data publikacji:
2013
Wydawca:
Instytut Medycyny Wsi
Tematy:
helping
harming
insulin-treated patient
diabetic patient
ambulatory treatment
diabetes mellitus
insulin therapy
body weight
hemoglobin
knowledge
behaviour
Opis:
Introduction. Correctness of insulin treatment depends on both the experience and skills of the doctor and knowledge and behaviour of the patient. Objective. Evaluation the adequacy of insulin doses administered to diabetes patients in ambulatory conditions. Material and methods. The treatment of 59 patients hospitalized in the Diabetology Ward was evaluated at admission, discharge and 3 months after hospitalization. Results. The mean daily doses of insulin significantly differed at times of evaluation and were: 53.90, 39.31 and 43.34 units, respectively (p≈0.000001). A significant reduction of body weight, 90.86 vs. 88.25 kg (p≈0.000001), was obtained only during hospitalization, and was maintained 3 months after discharge (87.86 kg). Significant differences were also noted in the body mass index (33.44 vs. 32.48 vs. 32.37 kg/m2, p≈0.000001). The change in waist circumference was not statistically significant (107.87 vs. 104.89 cm; p≈0.06). A decrease in the number of hypoglycaemia episodes was observed, but were statistically insignificant (25 vs. 23; p≈0.7). Three months after hospitalization an insignificant decrease of HbA1c level was noted (8.41% vs. 8.03%; p≈0.07). Conclusions. During treatment in the Diabetology Ward the procedure of choice was more frequently a reduction than an increase in insulin doses. This management led to the reduction of the patients’ body weight, improvement of glycaemia, without any significant effect on the diabetes control determined by the HbA1c level.
Źródło:
Annals of Agricultural and Environmental Medicine; 2013, 20, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Association of glycosylated hemoglobin with mortality of patients in intensive care unit: a prospective observation study
Autorzy:
Kumar, Damera Seshi
Wasnik, Seema Balkrishna
Yadav, Amlendu
Yadav, Rupesh
Powiązania:
https://bibliotekanauki.pl/articles/29432036.pdf
Data publikacji:
2024-03-31
Wydawca:
Towarzystwo Pomocy Doraźnej
Tematy:
Glycated hemoglobin
intensive care unit
mortality
Opis:
INTRODUCTION: Glycated hemoglobin (HbA1c) is the most commonly used clinical test to estimate mean blood glucose during the past 2 to 3 months. In addition to diagnostic purposes, the HbA1c level also predicts diabetes complications. The aim of this study was to determine the association of glycosylated hemoglobin with mortality in intensive care unit (ICU). MATERIALS AND METHODS: A prospective observational study was conducted in the ICU with a total of 281 patients. These patients were classified into two groups based on their HbA1c levels: one group with HbA1c level < 6.5 % and another group with HbA1c level ≥ 6.5%. The following data were collected during the study period. Clinical details and scores such as the APACHE II score (Acute Physiology and Chronic Health Assessment) and daily SOFA (Sequential Organ Failure Assessment) scores for the period of stay in the ICU. ICU morbidities as the need for mechanical ventilation, the use of inotropes / vasopressors, the length of stay in the ICU, and the requirement of renal replacement therapy (RRT). The outcome measures were ICU mortality and 28-day mortality. RESULTS: Of 281 patients admitted to the ICU for more than 48 hours, 157 patients (55.9%) had HbA1c levels < 6.5%, with the remaining 124 (44.1%) had levels ≥ 6.5%. ICU mortality was present in 107 (38.07%) cases. ICU mortality was higher in patients in the HbA1c ≥ 6.5% group compared to the HbA1c < 6.5% group. This was statistically significant (p-value <0.001). Mortality at 28 days was observed in 125 (44.48%) cases. Patients with an HbA1c value ≥ 6.5%, there was a higher mortality at 28 days compared to patients with an HbA1c value < 6.5%. This was found to be statistically significant (p-value <0.001). CONCLUSIONS: The study showed that glycated hemoglobin levels (HbA1c) levels ≥ 6.5% had a significantly higher mortality rate compared to the patient in the HbA1c level < 6.5%.
Źródło:
Critical Care Innovations; 2024, 7, 1; 24-33
2545-2533
Pojawia się w:
Critical Care Innovations
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biochemiczna rewolucja, czyli rzecz o Leonie Marchlewskim i Marcelim Nenckim
Biochemical revolution – some remarks about Leon Marchlewski’s and Marceli Nencki’s achievements
Autorzy:
Gryglewski, R. W.
Powiązania:
https://bibliotekanauki.pl/articles/172131.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Chemiczne
Tematy:
biochemia
chemia organiczna
chlorofil
hemoglobina
Marchlewski Leon
Nencki Marceli
biochemistry
organic chemistry
chlorophyll
hemoglobin
Opis:
Understanding of the fundamental law and mechanisms governing the phenomenon of life is an inherent feature of human civilization. With the birth of philosophy comes first speculation about the physical conditions of life processes, which consequently will lead to the formation of the first scientific theories. Among them an important role plays the Hippocratic humoral theory. This scientific rout will be taken by the next generations of researchers seeking for the most appropriate methods and precise language of science. A significant breakthrough came in the 16th century, when Paracelsus coined his philosophical and alchemical doctrine which gives such attention to perpetual changes in living organisms, then described on the basis of transmutation. Nearly three centuries later, in the early nineteenth century the polish physician and chemist Jędrzej Śniadecki introduced the concept of metabolism, based on the principles that are present in compounds and chemical reactions. Just a dozen years later, in 1828, Wöhler`s synthesis of urea gives birth to organic chemistry. Language of chemistry has become a tool for the description of biological phenomena, slowly building up physiological chemistry which shortly was turned to biochemistry. For a young science one of the first challenges was the level of the rudimental for the living organisms dyes, which rightly appeared as essential for the understanding of the chemical nature of the phenomena of life. From that point the studies on chlorophyll (Pelletier, Caventou, Shunck, Hoppe- -Seyler) and hemoglobin (Hünefeld, Funke, Hoppe-Seyler) become crucial topic. In this pioneering studies significant, and sometimes decisive role was to be played by Polish scientists (Teichmann, Marchlewski, Nencki, Zaleski). Especially a few years’ time and very intense cooperation of Leon Marchlewski and Marcel Nencki would bring momentous decision. Marchlewski’s bold hypothesis about the chemical unity on the level of the basic dyes in plant and animal worlds was fully confirmed in the experimental procedure and the results achieved by Nencki brought the solution to the problem of the chemical structure of hemin. Joint research of Polish scientists became the foundation of modern biochemistry and had changed the biological and medical sciences so deeply, that we can talk about “biochemical revolution”. The following paper is an evaluation of the speech held by me during X National Organic Chemistry Symposium – OSCO X, Lodz, April 16–18, 2015.
Źródło:
Wiadomości Chemiczne; 2015, 69, 5-6; 465-477
0043-5104
2300-0295
Pojawia się w:
Wiadomości Chemiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparing prevalence and characteristic of anemia in children with simple versus complex febrile seizures
Autorzy:
Momen, Ali Akbar
Asnafi, Ali Amin
Nikkhah, Ali
Reza Khalilian, Mohammad
Mahmudi, Mohammad
Da voodzadeh, Hannaneh
Powiązania:
https://bibliotekanauki.pl/articles/552868.pdf
Data publikacji:
2018
Wydawca:
Stowarzyszenie Przyjaciół Medycyny Rodzinnej i Lekarzy Rodzinnych
Tematy:
seizures
febrile
anemia
hemoglobin
platelet.
Źródło:
Family Medicine & Primary Care Review; 2018, 1; 25-28
1734-3402
Pojawia się w:
Family Medicine & Primary Care Review
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparison of risk factors for low birth weight among mothers with normal and low birth weight babies
Autorzy:
Mugada, V.
Kolakota, R.K.
Sakalabathula, A.
Kola, B.P.
Powiązania:
https://bibliotekanauki.pl/articles/2087912.pdf
Data publikacji:
2020
Wydawca:
Uniwersytet Opolski. Instytut Nauk o Zdrowiu
Tematy:
infant
low birth weight
hemoglobin
risk factors
anemia
India
Opis:
Background: Low birth weight is an alarming problem in developing countries and has severe future complications. Aim of the study: Our study aimed to compare the risk factors among mothers with low and normal birth weight babies. Material and methods: A cross-sectional study was carried out on 1000 mothers with normal and low birth weight babies (500 per group) over two years. Maternal parameters including age, hemoglobin levels, gravida, maternal weight gain, height, pregnancy-induced hypertension, etc., were collected along with anthropometric data of the child. We compared risk factors among the low and normal birth weight babies using the chi-square test, determining statistical significance at p<0.05, and high statistical significance at p<0.01. Results: Highly statistically significant associations were observed between low birth weight and seven variables: maternal age (p=0.0074), maternal height (p<0.0001), weight (p<0.0001), weight gain (p<0.0001), hemoglobin (p<0.0001), severe anemia (p<0.0001), and pregnancy-induced hypertension (p<0.0001). Conclusions: Our study observed significant modifiable risk factors like weight gain, weight, hemoglobin, and anemia among mothers with low birth weight babies. If we focus on raising awareness surrounding these risk factors, there may be an improvement in the birth weight of babies in this population.
Źródło:
Medical Science Pulse; 2020, 14, 2; 15-18
2544-1558
2544-1620
Pojawia się w:
Medical Science Pulse
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Determination of serum iron levels and hemoglobin concentration in pre-weaning calves
Autorzy:
Geboliszova, K.
Kadek, R.
Illek, J.
Powiązania:
https://bibliotekanauki.pl/articles/1192775.pdf
Data publikacji:
2021
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie / Polskie Towarzystwo Magnezologiczne im. Prof. Juliana Aleksandrowicza
Tematy:
calves
milk diet
iron
hemoglobin
Opis:
The aim of this study was to evaluate the serum iron (Fe) levels and hemoglobin (Hb) values in Holstein-Friesian calves with different types of milk diet. In total, 20 dairy calves (10 from each farm) were used for the experiment on two dairy farms. One farm provided the calves with a milk replacer twice a day from day 3 until weaning (farm 1), and the other one fed the calves native milk twice a day for three weeks before the milk replacer was administered (farm 2). Blood samples were collected three times, first at the age of 2-7 days, second time at the age of 30 days and last time at the age of 56 - 60 days, for the determination of iron and hemoglobin levels. Our experiment included monitoring of the occurrence of diarrheal and respiratory diseases in all calves. Values of serum iron and hemoglobin during the first sampling on farm 1: Fe 33.18 µmol l-1 (±29.39) and Hb 83.7 g l-1 (±11. 35); farm 2: Fe 8.38 µmol l-1 (±7.90) and Hb 90. 4 g l-1(±8.36). Second sampling on farm 1: Fe 37.35 µmol l-1 (±23.40) and Hb 106 g l-1 (±9.30); farm 2: Fe 5.62 µmol l-1 (±3.42) and Hb 80.3 g l-1 (±16.59). Last time on farm 1 the results were: Fe 42.69 µmol l-1 (±13.50) and Hb 101.0 g l-1 (±6.88); farm 2: Fe 32.79 µmol l-1 (±16. 07) and Hb 89.9 g l-1 (±6. 71). No respiratory diseases were observed on either farm during this study, diarrheal diseases occurred five times on farm 1 and nine times on farm 2. The results show that iron deficiency and lower hemoglobin concentration are more prevalent in animals fed whole milk.
Źródło:
Journal of Elementology; 2021, 26, 1; 261-270
1644-2296
Pojawia się w:
Journal of Elementology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of fenugreek seed supplementation on Hemoglobin and PCV among 20-30 years old females: a pilot study
Autorzy:
MP, Brundha
Powiązania:
https://bibliotekanauki.pl/articles/2203253.pdf
Data publikacji:
2022-05-04
Wydawca:
Gdański Uniwersytet Medyczny
Tematy:
Anemia
Hemoglobin
Fenugreek supplementation
PCV
Hematopoietic stem cells
Opis:
BackgroundFemales are more likely than males to suffer from anemia. There have been many studies investigating the nutritional qualities of traditional plants. The of this study was to assess the effect of fenugreek seeds on hemoglobin and PCV values in 20-30 years old females.Material and methodsBaseline hematological tests were obtained from 10 females aged 20-30 years. Supplementation of the standard amount of fenugreek was given to the population. After 48 days the same hematological parameters were analyzed as a post-test. The results of the pre- and post-test analysis were compared by using a paired-sample t-test. Relevant details were also collected and analyzed.ResultsThe mean value of hemoglobin before supplementation (Hb1) and hemoglobin after supplementation (Hb2) was 12.3200 and 11.3300 respectively and the mean value of packed cell volume before (PCV1) and after supplementation (PCV2) was 36.7970 and 35.4700, respectively. This shows that there is a mild decrease in hemoglobin and PCV values after fenugreek supplementation, but not at a statistically significant level.ConclusionsWithin the limitations of our pilot study, we conclude that fenugreek has no significant effect on Hemoglobin and PCV values and can’t be used as an exact supplement to cure anemia.
Źródło:
European Journal of Translational and Clinical Medicine; 2022, 5, 1; 27-32
2657-3148
2657-3156
Pojawia się w:
European Journal of Translational and Clinical Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of Medicago sativa Mhb1gene expression on defense response of Arabidopsis thaliana plants
Autorzy:
Maassen, Anna
Hennig, Jacek
Powiązania:
https://bibliotekanauki.pl/articles/1039900.pdf
Data publikacji:
2011
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Arabidopsis thaliana
pathogen infection
nitrotyrosine
nitric oxide
peroxidase activity
non-symbiotic hemoglobin
Opis:
Besides the previously described nitric oxide-detoxification activity we identified new features of class-1 non-symbiotic hemoglobin from Medicago sativa (Mhb1). Under in vitro conditions, using peroxidase in-gel activity assay, the Mhb1 protein was shown to possess also peroxidase-like activity. Due to this activity, in the presence of nitrite and hydrogen peroxide, the protein can mediate autonitration and nitration of other proteins at tyrosine residues, as revealed by tandem mass spectrometry and immune assay approaches. Mhb1 through its multifunctional activities can affect different components of signal transduction cascades operating during plant response to infections. This influence is manifested by Mhb1-mediated selective up-regulation of expression of certain pathogen inducible genes in Pseudomonas syringae infected Arabidopsis thaliana plants which overproduce Mhb1, as revealed by reverse transcription-quantitative real-time PCR analysis. Changes in expression level of these genes can influence such processes as synthesis of secondary metabolites, protein degradation and biosynthesis of ethylene. They can also result in alteration of pathogen-induced defense response of Mhb1 transgenic plants.
Źródło:
Acta Biochimica Polonica; 2011, 58, 3; 427-432
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of low doses of gamma rays on the stability of normal and diabetic erythrocytes
Autorzy:
Kaczmarska, Magdalena
Kopyściańska, Zofia
Fornal, Maria
Grodzicki, Tomasz
Matlak, Krzysztof
Korecki, Józef
Burda, Květoslava
Powiązania:
https://bibliotekanauki.pl/articles/1039830.pdf
Data publikacji:
2011
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
γ rays
diabetes
absorption spectroscopy
Mössbauer spectroscopy
hemoglobin
erythrocytes
hemolysis
Opis:
We studied the influence of low doses of γ radiation (from 0.04 to 1.8 mGy) on the stability of human red blood cells (RBC) from healthy donors and diabetic patients using absorption spectroscopy. Because of the alteration of many enzymatic pathways in diabetic RBCs resulting in strong modification of the lipid and protein membrane components one could expect that the ionizing γ-radiation should influence the stability of the healthy and diabetic cells in a different way. Indeed, distinct discontinuities and monotonic changes of hemolysis detected in the healthy and diabetic RBCs suggest that various enzymatic and chemical processes are activated in these membranes by γ radiation. Mössbauer measurements showed that only the highest applied dose of γ radiation caused modification of hemoglobin in both types of RBCs.
Źródło:
Acta Biochimica Polonica; 2011, 58, 4; 489-496
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Flavonoids as reductants of ferryl hemoglobin
Autorzy:
Gebicka, Lidia
Banasiak, Ewa
Powiązania:
https://bibliotekanauki.pl/articles/1040549.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
methemoglobin
ferryl hemoglobin
oxyhemoglobin
flavonoids
Opis:
The ferryl derivatives of hemoglobin are products of the reactions of oxy- and methemoglobin with hydrogen peroxide. Ferryl hemoglobins, either with or without a radical site on the protein moiety, are oxidizing species. Plant polyphenols, flavonoids, have been shown to act as antioxidants in vivo and in vitro. Reactions of met- and oxyhemoglobin with hydrogen peroxide in the presence of catechin, quercetin and rutin were studied. These flavonoids accelerated reduction of ferryl hemoglobin to methemoglobin. The rate constants of the reactions of ferryl hemoglobin with catechin, quercetin and rutin were in the order of 102 M-1 s-1, i.e. similar to the rate constants of ferryl hemoglobin with intracellular reducing compounds like urate or ascorbate. The beneficial effect of flavonoids against oxidative damage of hemoglobin caused by hydroperoxides, reported in the literature, is probably, at least in part, connected with the ability of flavonoids to scavenge ferryl hemoglobin.
Źródło:
Acta Biochimica Polonica; 2009, 56, 3; 509-513
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Haematological, blood gas and acid-base effects of central histamine-induced reversal of critical haemorrhagic hypotension in rats
Autorzy:
Jochem, J.
Powiązania:
https://bibliotekanauki.pl/articles/71043.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Fizjologiczne
Tematy:
histamine
hemoglobin
hematocrit
blood gas
erythrocyte
cardiovascular effect
hemorrhagic hypotension
hypotension
hematological parameter
rat
Źródło:
Journal of Physiology and Pharmacology; 2001, 52, 3
0867-5910
Pojawia się w:
Journal of Physiology and Pharmacology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
New generation of peptide antibiotics
Autorzy:
Dubin, Adam
Mak, Paweł
Dubin, Grzegorz
Rzychon, Małgorzata
Stec-Niemczyk, Justyna
Wladyka, Benedykt
Maziarka, Katarzyna
Chmiel, Dorota
Powiązania:
https://bibliotekanauki.pl/articles/1041366.pdf
Data publikacji:
2005
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
staphostatin
Staphylococcus
virulence factor
hemoglobin
protease inhibitor
hemocidins
antimicrobial agents
antibiotics
Opis:
The increasing antibiotic resistance of pathogenic bacteria calls for the development of alternative antimicrobial strategies. Possible approaches include the development of novel, broad-spectrum antibiotics as well as specific targeting of individual bacterial virulence factors. It is impossible to decide currently which strategy will prove more successful in the future since they both promise different advantages, but also introduce diverse problems. Considering both approaches, our laboratory's research focuses on the evaluation of hemocidins, broad-spectrum antibacterial peptides derived from hemoglobin and myoglobin, and staphostatins, specific inhibitors of staphopains - Staphylococcus aureus secreted proteases that are virulence factors regarded as possible targets for therapy. The article summarizes recent advances in both fields of study and presents perspectives for further development and possible applications.
Źródło:
Acta Biochimica Polonica; 2005, 52, 3; 633-638
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Opracowanie metody oznaczania aktywności pepsyny w soku żołądkowym
Development of method to assay pepsin activity in gastric juice
Autorzy:
Krywult, Anna
Długaszek, Michał
Szumska, Magdalena
Tyrpień-Golder, Krystyna
Wielkoszyński, Tomasz
Powiązania:
https://bibliotekanauki.pl/articles/1037654.pdf
Data publikacji:
2015
Wydawca:
Śląski Uniwersytet Medyczny w Katowicach
Tematy:
pepsyna
enzymy trawienne
hemoglobina ludzka
pepsin
digestive enzymes
human hemoglobin
Opis:
INTRODUCTION: The aim of this study was to develop a method to assay the activity of pepsin in gastric juice based on the method described in the references, which was not entirely effective in laboratory tests. The study aims to improve the aforementioned method based on the digestion of bovine hemoglobin by pepsin and to introduce effective changes so that the results may be useful for diagnostic purposes. MATERIALS AND METHODS: To achieve this, tests were carried out using the hemoglobin of different species of animals and human hemoglobin, changing the incubation periods, concentrations and volumes of reagents. RESULTS: Tests based on the use of purified (crystalline) pepsin revealed that the most effective in the assay of enzyme activity is human hemoglobin. The originally proposed incubation periods were extended, and in order to inhibit the reaction, trichloroacetic acid was used in a higher concentration than suggested, while reducing by half the volume of reactants. In the tests carried out using a purified enzyme, a correlation between the known concentration of the enzyme and the absorbance of supernatant containing the reaction products of enzymatic digestion of hemoglobin was proved. CONCLUSIONS: Introducting changes leads to more effective diagnostic use.
WSTĘP: Celem niniejszych badań było opracowanie metody oznaczania aktywności pepsyny w soku żołądkowym na podstawie metody opisanej w piśmiennictwie, która w badaniach laboratoryjnych okazała się nie w pełni efektywna. Podjęta praca jest próbą udoskonalenia metody polegającej na trawieniu hemoglobiny wołowej przez pepsynę i wprowadzenia skutecznych zmian, aby uzyskane wyniki mogły być użyteczne w diagnostyce klinicznej. MATERIAŁ I METODY: Przeprowadzono badania z wykorzystaniem hemoglobin różnych gatunków zwierząt i człowieka, zmiany czasów inkubacji, stężeń oraz objętości reagentów. WYNIKI: Próby oparte na wykorzystaniu roztworu pepsyny wykazały, że najskuteczniejsza w oznaczaniu aktywności enzymu jest hemoglobina ludzka. Pierwotnie proponowane czasy inkubacji zostały wydłużone, a do hamowania reakcji użyto kwasu trichlorooctowego o wyższym stężeniu, niż sugerowano, równocześnie zmniejszając o połowę objęto-ści reagentów. W próbach przeprowadzonych z użyciem oczyszczonego enzymu wykazano korelację między stężeniem enzymu a absorbancją supernatantu zawierającego produkty reakcji enzymatycznego trawienia hemoglobiny. WNIOSKI: Wprowadzone modyfikacje pozwalają na udoskonalenie procesu oznaczania aktywności pepsyny w soku żołądkowym i efektywnego wykorzystania w praktyce laboratoryjnej.
Źródło:
Annales Academiae Medicae Silesiensis; 2015, 69; 85-90
1734-025X
Pojawia się w:
Annales Academiae Medicae Silesiensis
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 26

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