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    Wyświetlanie 1-4 z 4
    Tytuł:
    Differences in metabolic profiles of planktonic and biofilm cells in Staphylococcus aureus - 1H Nuclear Magnetic Resonance search for candidate biomarkers
    Autorzy:
    Junka, Adam
    Deja, Stanisław
    Smutnicka, Danuta
    Szymczyk, Patrycja
    Ziółkowski, Grzegorz
    Bartoszewicz, Marzenna
    Młynarz, Piotr
    Powiązania:
    https://bibliotekanauki.pl/articles/1039470.pdf
    Data publikacji:
    2013
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Staphylococcus aureus
    biofilm
    NMR
    metabolomics
    glycine-betaine
    Opis:
    Staphylococcus aureus is responsible for many types of infections related to biofilm presence. As the early diagnostics remains the best option for prevention of biofilm infections, the aim of the work presented was to search for differences in metabolite patterns of S. aureus ATCC6538 biofilm vs. free-swimming S. aureus planktonic forms. For this purpose, Nuclear Magnetic Resonance (NMR) spectroscopy was applied. Data obtained were supported by means of Scanning Electron Microscopy, quantitative cultures and X-ray computed microtomography. Metabolic trends accompanying S. aureus biofilm formation were found using Principal Component Analysis (PCA). Levels of isoleucine, alanine and 2,3-butanediol were significantly higher in biofilm than in planktonic forms, whereas level of osmoprotectant glycine-betaine was significantly higher in planktonic forms of S. aureus. Results obtained may find future application in clinical diagnostics of S. aureus biofilm-related infections.
    Źródło:
    Acta Biochimica Polonica; 2013, 60, 4; 701-706
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effects of pH on the activity and structure of choline oxidase from Alcaligenes species
    Autorzy:
    Hekmat, Azadeh
    Saboury, Ali
    Moosavi-Movahedi, Ali
    Ghourchian, Hedayatollah
    Ahmad, Faizan
    Powiązania:
    https://bibliotekanauki.pl/articles/1040713.pdf
    Data publikacji:
    2008
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    optimum pH
    choline oxidase
    nile red
    glycine-betaine
    Opis:
    A reversible effect of pH on the ionization of amino-acid residues at the active center of choline oxidase was observed near the optimum pH (8). Inactivation of choline oxidase took place in the pH ranges 3-6 and 9-11, in which irreversible changes in the structure occur leading to the enzyme inactivation. The first order rate constants of the enzyme's inactivation at various pH values were estimated for the irreversible changes. The Arrhenius analysis revealed no significant changes in the activation enthalpy, while an increase in the activation entropy reflected an increase in the conformational freedom.
    Źródło:
    Acta Biochimica Polonica; 2008, 55, 3; 549-557
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effects of foliar application of cobalt oxide nanoparticles on growth, photosynthetic pigments, oxidative indicators, non-enzymatic antioxidants and compatible osmolytes in canola (Brassica napus L.)
    Autorzy:
    Jahani, M.
    Khavari-Nejad, R.A.
    Mahmoodzadeh, H.
    Saadatmand, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/2117869.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    ascorbate and glutathione
    carbohydrate
    environmental contaminants
    glycine betaine
    lipoxygenase activity
    total flavonoids and flavonols
    toxicity and beneficial assessment
    Opis:
    Nanotechnology has been widely applied in agriculture, and understanding of the mechanisms of plant interaction with nanoparticles (NPs) as environmental contaminants is important. The aim of this study was to determine the effects of foliar application of cobalt oxide (Co 3O4) NPs on some morpho-physiological and biochemical changes of canola (Brassica napus L.) leaves. Seeds were sown in plastic pots and grown under controlled conditions. Fourteen-day-old seedlings were sprayed with different concentrations of Co3O4 NPs (0, 50, 100, 250, 500, 1000, 2000, and 4000 mg L -1 ) at weekly intervals for 5 weeks. Growth parameters of the shoot (length, fresh and dry weights) were stimulated by low concentrations of Co 3O4 NPs (50 and 100 mg L -1 ) and repressed by higher con- centrations. Similar trends were observed in photosynthetic pigment contents. The results indicated that high concentrations of Co3O4 NPs increased lipoxygenase (LOX) activity and the malondialdehyde (MDA), hydrogen per- oxide (H 2O2), and dehydroascorbate (DHA) contents, but reduced the membrane stability index (MSI), ascorbate (ASC), and glutathione (GSH). Despite the increase of antioxidant capacity (DPPH) and the accumulation of non- enzymatic antioxidants (total flavonoids and flavonols) and osmolytes (proline, glycine betaine (GB) and soluble sugars) at high concentrations of Co 3O4 NPs, the growth and photosynthesis were reduced. The defence system activity did not seem to be sufficient to detoxify reactive oxygen species (ROS). Altogether, high concentrations of Co3O4 NPs showed a phytotoxic potential for canola as an oilseed crop.
    Źródło:
    Acta Biologica Cracoviensia. Series Botanica; 2019, 61, 1; 29-42
    0001-5296
    Pojawia się w:
    Acta Biologica Cracoviensia. Series Botanica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Chalcopyrite leaching by amino acid solutions in the presence of hydrogen peroxide
    Autorzy:
    Nurtazina, Nargiza
    Uvarov, Nikolai
    Azhigulova, Ryskul
    Tyapkin, Pavel
    Powiązania:
    https://bibliotekanauki.pl/articles/2175441.pdf
    Data publikacji:
    2022
    Wydawca:
    Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
    Tematy:
    chalcopyrite
    amino acid leaching
    glycine
    betaine
    lysine
    Opis:
    It is demonstrated that amino acids such as glycine, betaine, and lysine may be applied as selective copper leaching agents in the process of hydrochemical oxidation of chalcopyrite at atmospheric pressure and in the temperature range 25÷65 °C. The advantage of the proposed method is its relative environmental friendliness. Solid samples of the initial mineral and leached chalcopyrite residues were analyzed by X-ray diffraction analysis, and Mössbauer and Raman spectroscopy. The results of the atomic absorption analysis showed that copper's extraction efficiency increased when the experiment's duration was raised and when the process temperature was higher. The leaching ability of amino acids was evaluated by the specific leaching rate of chalcopyrite in these reagents. Amino acid leaching was thought to be mainly due to the complexation of copper (II) glycinates, copper (II) betainates and copper (II) lysinates. This reaction can be enhanced by adding hydrogen peroxide to the system as an oxidant. It was determined that glycine was more effective as a leaching agent than betaine and lysine. Based on experimental data, it was assumed that the most possible mechanism is selective copper leaching due to the formation of chalcopyrite-based solid solution Cu1-XFeS2.
    Źródło:
    Physicochemical Problems of Mineral Processing; 2022, 58, 6; art. no. 157067
    1643-1049
    2084-4735
    Pojawia się w:
    Physicochemical Problems of Mineral Processing
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-4 z 4

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