Temat: glutathione - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Glutathione level and glutathione reductase activity in serum of coronary heart disease patients
Autorzy:: Zuzak, Ewa
Horecka, Anna
Kiełczykowska, Małgorzata
Dudek, Aneta
Musik, Irena
Kurzepa, Joanna
Kurzepa, Jacek
Powiązania:: https://bibliotekanauki.pl/articles/972495.pdf
Data publikacji:: 2017
Wydawca:: Instytut Medycyny Wsi
Tematy:: glutathione reductase
glutathione
coronary heart disease
oxidative stress
Opis:: Introduction. Oxidative stress induced by the generation of excess reactive oxygen species is one of the cause of atherosclerosis finally leading to coronary heart disease (CHD). Glutathione reductase (GR), a flavoprotein antioxidant enzyme, regenerates glutathione (GSH) from its oxidized form, which is essential for scavenging of hydrogen peroxide by glutathione peroxidase. The aim of this study was to analyze the activity of GR and GSH level in serum of patients with various stages of CHD. Materials and method. Sixty consecutive patients with diagnosis of stable angina (SA, n=20), unstable angina (UA, n=20) and non-fatal myocardial infarction (MI, n=20) together with 20 healthy control subjects were enrolled. Venous blood samples were collected just before the coronary catheterization procedure (in study patients). GR activities and GSH concentration was determined using commercially available colorimetric kits. Results. The highest GR activity and GSH serum level was noticed in UA patients. However, statistical evaluation showed that only glutathione reductase activity was significantly higher in serum obtained from myocardial infarction patients, compared to stable angina pectoris patients (19.52 ± 13.88 vs 11.63 ± 5.45 nmol/min/ml for MI and SA, respectively; p<0.048). There were no significant differences in GSH between the CHD patients and controls. Conclusion. The elevated activity of glutathione reductase in serum of patients with unstable angina pectoris and myocardial infarction suggests the role of antioxidant system acute coronary syndromes.
Źródło:: Journal of Pre-Clinical and Clinical Research; 2017, 11, 2; 103-105
1898-2395
Pojawia się w:: Journal of Pre-Clinical and Clinical Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Amentoflavone prevents sepsis-associated acute lung injury through Nrf2-GCLc-mediated upregulation of glutathione
Autorzy:: Zong, Yuan
Zhang, Huali
Powiązania:: https://bibliotekanauki.pl/articles/1038691.pdf
Data publikacji:: 2017
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: Sepsis
Acute lung injury
Amentoflavone
Nrf2
Glutathione
Opis:: Sepsis is a serious medical problem and is one of the main causes of high mortality in intensive care units. Fifty percent of patients with severe sepsis will develop acute lung injury (ALI). Amentoflavone (AMF) is a polyphenolic compound possessing potent anti-inflammatory activities. The study aimed to explore the protective effects of AMF against ALI in cecal ligation and puncture (CLP)-induced septic rats. The results showed that AMF administration protected against septic ALI, as reflected by marked amelioration of histological injury of lung tissues and decrease of pulmonary edema in CLP-treated rats. AMF ameliorated CLP-induced increase of systemic and lung TNFα and IL-1β and binding activity of p65 NF-κB, indicating the inhibition of inflammation. Moreover, AMF prevented CLP-induced oxidative stress, as evidenced by increase of oxygen consumption rate, decrease of TBARS content, increase of SOD activity and GSH level in lung tissue of CLP-treated rats. CLP resulted in significant decrease of mRNA expression of Nrf2 and GCLc, which was inhibited by AMF. AMF-induced protective effects on ALI, inflammation, and oxidative stress were inhibited by lentivirus shRNA-mediated silence of Nrf2 and buthionine sulphoximine (BSO), an inhibitor of GSH synthesis. AMF increased Nrf2-binding activity in GCLc promoters in lung tissue of CLP-treated rats. The results suggested that AMF protected against ALI in septic rats through upregulation of Nrf2-GCLc signaling, enhancement of GSH antioxidant defense, reduction of oxidative stress and final amelioration of inflammation and histological injury of lung. The data provide new therapeutic options for the treatment of sepsis-associated ALI.
Źródło:: Acta Biochimica Polonica; 2017, 64, 1; 93-98
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Subcellular antioxidative defense of plants during abiotic stress
Autorzy:: Zechmann, B.
Powiązania:: https://bibliotekanauki.pl/articles/81187.pdf
Data publikacji:: 2013
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: conference
antioxidant
ascorbate
glutathione
Arabidopsis thaliana
reactive oxygen species
redox signalling
gene expression
oxidative stress
abiotic stress
physiological condition
plant
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Changes in GSH-antioxidant system induced by daunorubicin in human normal and diabetic fibroblasts.
Autorzy:: Zatorska, Agnieszka
Maszewski, Janusz
Jóźwiak, Zofia
Powiązania:: https://bibliotekanauki.pl/articles/1043460.pdf
Data publikacji:: 2003
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: glutathione S-transferase
glutathione reductase
glutathione peroxidase
daunorubicin
apoptosis
glutathione
oxidative stress
Opis:: We investigated the effect of daunorubicin on glutathione content and activity of GSH-related enzymes in cultured normal and diabetic human fibroblasts. Cells were incubated with 4 μM daunorubicin (DNR) for 2 h followed by culture in drug-free medium for up to 72 h. Treatment of diabetic cells with the drug caused a time-dependent depletion of intracellular GSH and a decrease of the GSH to total glutathione ratio. GSH depletion was accompanied by apoptotic changes in morphology of the nucleus. Analysis of GSH-related enzymes showed a significant increase of the activities of Se-dependent and Se-independent peroxidases and glutathione S-transferase. In contrast, glutathione reductase activity was reduced by 50%. Significant differences between normal and diabetic cells exposed to DNR were observed in the level of GST and Se-dependent glutathione peroxidase activities. These findings indicated that daunorubicin efficiently affects the GSH antioxidant defense system both in normal and diabetic fibroblasts leading to disturbances in glutathione content as well as in the activity of GSH-related enzymes.
Źródło:: Acta Biochimica Polonica; 2003, 50, 3; 825-835
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: Red blood cell and plasma glutathione peroxidase activities and selenium concentration in patients with chronic kidney disease: A review
Autorzy:: Zachara, Bronisław
Gromadzińska, Jolanta
Wąsowicz, Wojciech
Zbróg, Zbigniew
Powiązania:: https://bibliotekanauki.pl/articles/1041155.pdf
Data publikacji:: 2006
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: hemodialysis
selenium
kidney transplantation
glutathione peroxidase
chronic kidney disease
antioxidants
plasma
Opis:: The metabolism of oxygen in aerobic organisms leads to generation of reactive oxygen species (ROS). These entities are able to oxidize almost all classes of macromolecules, including proteins, lipids and nucleic acids. The physiological level of ROS is usually regulated by antioxidant defense mechanisms. There are at least three groups of antioxidant enzymes: superoxide dismutases, catalases and glutathione peroxidases (GSH-Pxs) which neutralize ROS. The trace elements (copper, zinc and selenium) bound to the active sites of the above listed enzymes play an important role in the antioxidant defense system. In mammals, a major function of selenium (Se) and Se-dependent GSH-Pxs is to protect cells from oxidative stress. Selenium concentrations and GSH-Px activities are altered in blood components of chronic kidney disease (CKD) patients. The Se level is frequently lower than in healthy subjects and the concentration very often decreases gradually with advancing stage of the disease. Studies on red cell GSH-Px activity in CKD patients reported its values significantly lower, significantly higher and lower or higher, but not significantly as compared with healthy subjects. On the other hand, all authors who studied plasma GSH-Px activity have shown significantly lower values than in healthy subjects. The degree of the reduction decreases gradually with the progression of the disease. High inverse correlations were seen between plasma GSH-Px activity and creatinine level. A gradual decrease in plasma GSH-Px activity in CKD patients is due to the fact that this enzyme is synthesized predominantly in the kidney and thus the impairment of this organ is the cause of the enzyme's lower activity. Se supplementation to CKD patients has a slightly positive effect in the incipient stage of the disease, but usually no effect was observed in end-stage CKD. Presently, kidney transplantation is the only treatment that may restore plasma Se level and GSH-Px activity in patients suffering from end-stage CKD. A few studies have shown that in kidney recipients, plasma Se concentration and GSH-Px activity are restored to normal values within a period of 2 weeks to 3 months following surgery and thus it can be acknowledged that Se supplementation to those patients has a positive effect on plasma GSH-Px activity.
Źródło:: Acta Biochimica Polonica; 2006, 53, 4; 663-677
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 6.

Tytuł:: Selenium supplementation to chronic kidney disease patients on hemodialysis does not induce the synthesis of plasma glutathione peroxidase
Autorzy:: Zachara, Bronislaw
Gromadzinska, Jolanta
Zbrog, Zbigniew
Swiech, Rafal
Wasowicz, Wojciech
Twardowska, Ewa
Jablonska, Ewa
Sobala, Wojciech
Powiązania:: https://bibliotekanauki.pl/articles/1040656.pdf
Data publikacji:: 2009
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: hemodialysis
selenium supplementation
glutathione peroxidase
chronic kidney disease
plasma
Opis:: Background: Numerous authors have shown that selenium (Se) concentration and glutathione peroxidase (GSH-Px) activity in plasma of chronic kidney disease (CKD) patients are lower than in healthy subjects, but there are only few publications on the level of GSH-Px protein in those patients and no reports on the effect of Se supplementation to HD patients on the level of this enzyme. Subjects and Methods: Se concentration and GSH-Px protein level in plasma were measured in a group of 30 CKD patients on hemodialysis (HD) supplemented with 200 µg Se/day for 3 months, and 28 patients on HD administered with placebo. Se concentration was measured by graphite furnace atomic absorption spectrometry and plasma GSH-Px protein level by the sandwich ELISA method using polyclonal antibody specific for human plasma GSH-Px. Results: Se concentration in patients on placebo did not change throughout the 3-month study period, but increased significantly in Se supplemented group. Se supplementation to CKD patients on HD had no effect on the level of GSH-Px protein. Conclusions: The lack of GSH-Px protein in CKD patients on HD is not linked to Se deficiency since the level of this element increased after Se supplementation while enzyme protein level did not change. The damaged kidney of HD patients is unable to synthesize GSH-Px, even after induction with selenium.
Źródło:: Acta Biochimica Polonica; 2009, 56, 1; 183-187
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 7.

Tytuł:: Effect of priming of seeds of Medicago sativa "Bami" with gibberellic acid on germination, seedlings growth and antioxidant enzymes activity under salinity stress
Autorzy:: Younesi, O.
Moradi, A.
Powiązania:: https://bibliotekanauki.pl/articles/1828.pdf
Data publikacji:: 2014
Wydawca:: Instytut Ogrodnictwa
Tematy:: salinity
seed priming
Medicago sativa
gibberellic acid
germination
seedling growth
growth
antioxidative enzyme
alfalfa
guaiacol peroxidase
catalase
ascorbate peroxidase
glutathione reductase
Źródło:: Journal of Horticultural Research; 2014, 22, 2
2300-5009
Pojawia się w:: Journal of Horticultural Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 8.

Tytuł:: Lipid peroxidation and cell cycle signaling : 4-hydroxynonenal, a key molecule in stress mediated signaling.
Autorzy:: Yang, Yusong
Sharma, Rajendra
Sharma, Abha
Awasthi, Sanjay
Awasthi, Yogesh
Powiązania:: https://bibliotekanauki.pl/articles/1043608.pdf
Data publikacji:: 2003
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: glutathione S-transferase
RLIP76
4-hydroxynonenal
RalBP1
cell cycle signaling
apoptosis
Opis:: Role of lipid peroxidation products, particularly 4-hydroxynonenal (4-HNE) in cell cycle signaling is becoming increasingly clear. In this article, recent studies suggesting an important role of 4-HNE in stress mediated signaling for apoptosis are critically evaluated. Evidence demonstrating the modulation of UV, oxidative stress, and chemical stress mediated apoptosis by blocking lipid peroxidation by the α-class glutathione S-transferases (GSTs) is presented which suggest an important role of these enzymes in protection against oxidative stress and a role of lipid peroxidation products in stress mediated signaling. Overexpression of 4-HNE metabolizing GSTs (mGSTA4-4, hGSTA4-4, or hGST5.8) protects cells against 4-HNE, oxidative stress (H2O2 or xanthine/xanthine oxidase), and UV-A mediated apoptosis by blocking JNK and caspase activation suggesting a role of 4-HNE in the mechanisms of apoptosis caused by these stress factors. The intracellular concentration of 4-HNE appears to be crucial for the nature of cell cycle signaling and may be a determinant for the signaling for differentiation, proliferation, transformation, or apoptosis. The intracellular concentrations of 4-HNE are regulated through a coordinated action of GSTs (GSTA4-4 and hGST5.8) which conjugate 4-HNE to GSH to form the conjugate (GS-HNE) and the transporter 76 kDa Ral-binding GTPase activating protein (RLIP76), which catalyze ATP-dependent transport of GS-HNE. A mild stress caused by heat, UV-A, or H2O2 with no apparent effect on the cells in culture causes a rapid, transient induction of hGST5.8 and RLIP76. These stress preconditioned cells acquire ability to metabolize and exclude 4-HNE at an accelerated pace and acquire relative resistance to apoptosis by UV and oxidative stress as compared to unconditioned control cells. This resistance of stress preconditioned cells can be abrogated by coating the cells with anti-RLIP76 antibodies which block the transport of GS-HNE. These studies and previous reports discussed in this article strongly suggest a key role of 4-HNE in stress mediated signaling.
Źródło:: Acta Biochimica Polonica; 2003, 50, 2; 319-336
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 9.

Tytuł:: Selected biochemical parameters of macrophytes applied in the Model Biofiltering System (MBS)
Autorzy:: Wyrwicka, A.
Kiedrzynska, E.
Kiedrzynski, M.
Urbaniak, M.
Materac, M.
Sklodowska, M.
Powiązania:: https://bibliotekanauki.pl/articles/80510.pdf
Data publikacji:: 2013
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: conference
biochemical parameter
macrophyte
water purification
heavy metal
model biofiltering system
waste water
Pilica catchment
glutathione transferase
guaiacol peroxidase
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 10.

Tytuł:: Analysis of the transcriptome of Potentilla sericea under cadmium stress conditions
Autorzy:: Wu, J.
Fan, W.
Gao, P.
Yang, Q.
Zhang, J.
Wang, L.
Powiązania:: https://bibliotekanauki.pl/articles/2130579.pdf
Data publikacji:: 2021
Wydawca:: Polskie Towarzystwo Botaniczne
Tematy:: glutathione metabolism
sulfur metabolism
Opis:: Cadmium (Cd) stress significantly affects plant growth and development. Potentilla sericea is typically grown in gardens or as ground cover. In this study, the Cd response of P. sericea was analyzed based on physiological examinations and transcriptome analyses that uncovered the gene expression changes in P. sericea roots induced by a 7-day treatment with 90 μmol/L Cd2+. A total of 53,225 unigenes were identified, including 11,684 differentially expressed genes (DEGs; 8,083 upregulated and 3,601 downregulated). Additionally, 44 gene ontology terms and 127 Kyoto Encyclopedia of Genes and Genomes pathways were significantly enriched among these DEGs. Genes related to glutathione metabolism, plant hormone signal transduction, peroxisome processes, sulfur metabolism, and flavonoid biosynthesis pathways were confirmed as relevant to the Cd response of P. sericea. The molecular biology-related data described here may be useful for the future breeding of transgenic P. sericea plants with increased resistance to heavy metal stresses.
Źródło:: Acta Societatis Botanicorum Poloniae; 2020, 89, 4
0001-6977
2083-9480
Pojawia się w:: Acta Societatis Botanicorum Poloniae
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 11.

Tytuł:: The effect of seedling chilling on glutathione content, catalase and peroxidase activity in Brassica oleracea L. var. italica
Autorzy:: Wojciechowska, R.
Hanus-Fajerska, E.J.
Kolton, A.
Kaminska, I.
Grabowska, A.
Kunicki, E.
Powiązania:: https://bibliotekanauki.pl/articles/57934.pdf
Data publikacji:: 2013
Wydawca:: Polskie Towarzystwo Botaniczne
Tematy:: seedling
chilling stress
glutathione content
catalase activity
peroxidase activity
Brassica oleracea var.italica
broccoli
development stage
Opis:: The study was designed to determine the possible relationship between Brassica oleracea var. italica seedlings stored at 2°C in the dark for seven and fourteen days, respectively, and the level of certain antioxidant parameters in particular organs. A parallel objective of the experiment was to determine if the reaction of seedlings to low temperature might be persistent in fully developed plants until harvest time. After 14 days of chilling a significant increase in the glutathione content was observed in the seedling leaves in comparison to the non-chilled plants. During vegetation in field conditions this effect was maintained in leaves up to the stage of formation of flower buds. At harvest the highest content of glutathione was demonstrated in broccoli heads, obtained from plants, which were previously chilled in the seedling phase for two weeks. Peroxidase activity in broccoli seedlings increased each year of the three-year study due to the duration of the cooling time, whereas in the case of catalase the changes were not so distinct. At harvest time the activity of both enzymes in the leaves and flower buds fluctuated according to the particular year of study.
Źródło:: Acta Societatis Botanicorum Poloniae; 2013, 82, 3
0001-6977
2083-9480
Pojawia się w:: Acta Societatis Botanicorum Poloniae
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 12.

Tytuł:: The γ-glutamyltransferase activity and non-protein sulfhydryl compounds levels in rat kidney of different age groups.
Autorzy:: Włodek, Przemysław
Sokołowska, Maria
Smoleński, Olgierd
Włodek, Lidia
Powiązania:: https://bibliotekanauki.pl/articles/1043788.pdf
Data publikacji:: 2002
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: L-cysteine
kidney
aging
γ-glutamyl transferase
reactive oxygen species
protein-bound cysteine
glutathione
sulfane sulfur compounds
Opis:: The present work was aimed to obtain information about age-dependent changes of γ-glutamyltransferase (GGT) activity and the levels of non-protein sulfhydryl compounds (NPSH) in rat kidneys. In addition, protein-bound cysteine (PB-Cys), sulfane sulfur compounds and reactive oxygen species (ROS) were estimated The results indicate that the activity of GGT and NPSH levels in the kidneys are reduced with age. At the same time, a significant increase in the level of protein-bound cysteine was observed. Simultaneously, the content of sulfane sulfur compounds was increased in the group of the oldest animals. These findings indicate that the capacity for extracellular glutathione degradation and, in consequence, the availability of cysteine for intracellular glutathione biosynthesis may be impaired. The increased PB-Cys level indicates potentiation of the thiolation reaction, i.e. development of protein-mixed disulfides. These results reveal age dependent disturbances in the thiol-disulfide equilibrium in the kidneys which leads to an imbalance between pro- and antioxidatory processes.
Źródło:: Acta Biochimica Polonica; 2002, 49, 2; 501-507
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 13.

Tytuł:: Wpływ zmian ciśnienia otoczenia na aktywność peroksydazy glutationowej (GPX) i atalazy (CAT) we krwi nurków : badania wstępne
The effect of ambient pressure changes on the activity of glutathione peroxidase (GPX) and catalase (CAT) in the blood divers : preliminary results
Autorzy:: Włodarski, A.
Woźniak, A.
Mila-Kierzenkowska, C.
Sutkowy, P.
Powiązania:: https://bibliotekanauki.pl/articles/366531.pdf
Data publikacji:: 2013
Wydawca:: Polskie Towarzystwo Medycyny i Techniki Hiperbarycznej
Tematy:: ciśnienie
nurkowanie
peroksydaza glutationowa
katalaza
pressure
diving
glutathione peroxidase
catalase
Opis:: Zmiana warunków otoczenia jest stresem dla organizmu, który może prowadzić do wzmożonej generacji reaktywnych form tlenu i licznych uszkodzeń w obrębie komórek. Istotną rolę w przeciwdziałaniu tym uszkodzeniom pełnią enzymy antyoksydacyjne. Celem niniejszej pracy było określenie wpływu zmian ciśnienia otoczenia w warunkach nurkowania na aktywność peroksydazy glutationowej oraz katalazy. W badaniu wzięło udział 11 płetwonurków w wieku od 18 do 41 lat. Badani spędzili 40 min w jeziorze, w wodzie o temperaturze 13° C, schodząc maksymalnie na głębokość około 9 m. Podczas zanurzenia wszyscy oddychali powietrzem. Krew do badań pobrano z żyły odłokciowej dwukrotnie: przed zanurzeniem w wodzie (kontrola) oraz bezpośrednio po wynurzeniu. W pracy wykazano tendencję do wzrostu aktywności katalazy oraz tendencję do obniżania się aktywności peroksydazy glutationowej, ale zmiany te nie były istotne statystycznie. Ciśnienie otoczenia wywierane przez wodę podczas nurkowania nie ma istotnego wpływu na aktywność peroksydazy glutationowej oraz katalazy w erytrocytach. Może to sugerować, że nie doszło do zwiększonej generacji nadtlenku wodoru będącego substratem reakcji katalizowanych przez oba enzymy. Brak istotnych statystycznie zmian aktywności badanych enzymów antyoksydacyjnych może być efektem zmian przystosowawczych w organizmie osób nurkujących.
The changes in ambient conditions are usually stress factor for the organism, which may lead to increased generation of reactive oxygen species and numerous damages within the cells. The important role in counteracting of free radical damages to cells is played by antioxidant enzymes. The aim of this study was to determine the impact of changes in ambient pressure during diving on activity of selected antioxidant enzymes: glutathione peroxidase and catalase . The study involved eleven divers, aged from 18 to 41 years. Subjects spent 40 minutes in the lake, in water at 13° C, going down to a depth of about 9 m. All divers breathe with the air. Blood samples were taken from basilica vein twice: before immersion in water (control) and immediately after surfacing. The study showed an increasing trend in catalase activity and trend to decrease in glutathione peroxidase activity, but the changes were not statistically significant. Ambient pressure exerted by the water during diving has no significant effect on the activity of glutathione peroxidase and catalase in erythrocytes. This may suggest, that there was no increased generation of hydrogen peroxide which is a substrate of the reaction catalyzed by two enzymes. No statistically significant differences in the activity of antioxidant enzymes could be due to adaptive changes in the body of divers.
Źródło:: Polish Hyperbaric Research; 2013, 1(42); 7-25
1734-7009
2084-0535
Pojawia się w:: Polish Hyperbaric Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 14.

Tytuł:: Determination of the tolerance of sunflower to lead-induced stress
Autorzy:: Winska-Krysiak, M.
Koropacka, K.
Gawronski, S.
Powiązania:: https://bibliotekanauki.pl/articles/15865.pdf
Data publikacji:: 2015
Wydawca:: Uniwersytet Warmińsko-Mazurski w Olsztynie / Polskie Towarzystwo Magnezologiczne im. Prof. Juliana Aleksandrowicza
Tematy:: lead
phytoremediation
determination
plant tolerance
sunflower
Helianthus annuus
glutathione
metallothionein gene
lead stress
Opis:: Six-week old sunflower seedlings, cv. Ogrodowy, were treated with 0, 15, 45 and 60 mg Pb dm-3, and then the content of lead and selected physiological and biochemical parameters were measured. Photosynthesis efficiency, water relations (intensity of transpiration, relative water content (RWC)) and gene-encoding metallothionein were measured three times after 24, 48 and 72 hours of exposure to Pb. The content of glutathione and lead was analysed after 72 hours’ exposure to Pb. Most of the lead uptake was accumulated in the roots, then in the stems and leaves, but when re-calculated per plant dry weight, the uptake of the metal did not depend on the lead dose applied. The highest 60 mg Pb dm-3 treatment was accompanied by a significant decrease in dry weight content. Moreover, most of the lead taken up in these plants was transported to the stems and leaves (23.6% of total lead uptake). The lead doses used in this study did not affect the intensity of photosynthesis, but a decrease in transpiration and relative water content was observed. The glutathione level in the plants varied depending on the organ examined and the Pb concentration in the treatment. The expression of the metallothionein gene HaMT1 was observed in the stems only. These results indicate that the sunflower cultivar Ogrodowy is a promising plant for phytoremediation of lead-polluted soils.
Źródło:: Journal of Elementology; 2015, 20, 2
1644-2296
Pojawia się w:: Journal of Elementology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 15.

Tytuł:: Lead tolerance mechanisms in Robinia pseudoaccacia L. - an attempt to a practical approach
Mechanizmy tolerancji na ołów u Robinia pseudoaccacia L. i ich aspekt praktyczny
Autorzy:: Wińska-Krysiak, M.
Bernat, J.
Powiązania:: https://bibliotekanauki.pl/articles/11541679.pdf
Data publikacji:: 2008
Wydawca:: Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:: Lead tolerance
tolerance mechanism
Robinia pseudoaccacia
practical approach
lead
glutathione
phytochelatin
root
RpPCS gene
RpGSH1 gene
soil contamination
Opis:: Robinia pseudoaccacia plants grown hydroponically were treated Pb(NO3)2 with 15, 45 mg Pb²⁺ ∙ dm⁻³. After 6, 12, 24, 72 hours of the metal treatment the plants were collected and dissected organs. The plants accumulated and transported to ground part 0.88% and 1.35% of total accumulated lead for the lower and higher dose of Pb²⁺ respectively. The level of GSH was differed and depended on organs, dose and time treatment of Pb²⁺. We investigate (different pattern of expression) expression of RpGSH1 and RpPCS genes in roots. The study showed that glutathione and genes encoded enzymes connected with synthesis of him, plays important role in the process of detoxification in plant.
Rośliny robinii akacjowej uprawianej w hydroponice traktowane były Pb(NO3)2 w ilościach: 0, 15, 45 mg Pb²⁺ ∙ dm⁻³. Materiał roślinny zbierano po 6, 12, 24 i 72 godzinach od traktowania ołowiem i rozdzielono na organy. Procent pobranego ołowiu w przeliczeniu na suchą masę roślin przetransportowany do części nadziemnej wynosił 0,88 dla najwyższej dawki traktowania oraz 1,35 dla niższej. Poziom glutationu był zróżnicowany i zależał od badanego organu, dawki i czasu od potraktowania ołowiem. Odnotowano zróżnicowaną ekspresję RpGSH1 i RpPCS w korzeniach roślin. Badania wykazały, że glutation i geny szlaku jego biosyntezy odgrywają ważną rolę w procesie detoksykacji ołowiu w roślinie.
Źródło:: Acta Scientiarum Polonorum. Hortorum Cultus; 2008, 07, 3; 77-86
1644-0692
Pojawia się w:: Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:: Biblioteka Nauki

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